重金属复合污染土壤中耐铅镉微生物的筛选及鉴定
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摘要
为获得复合重金属高耐性微生物资源菌株,采集广西桂西北尾矿区重金属污染的土壤样品,采用改良的马丁氏培养基分离和筛选既耐铅又耐镉的高耐性微生物.结果获得了一株耐铅浓度为1 200 mg/L、镉浓度为120 mg/L的铅镉复合耐性真菌——菌株K-3.该菌株(K-3)最适生长环境为温度30℃、p H值7.0、盐浓度3%.通过形态结合分子生物学鉴定得知,K-3菌株与已知分类地位的标准菌株Uncultured Westerdykella的18S rDNA序列一致性为99%.本研究获得的铅镉复合耐性真菌——菌株K-3是重金属铅和镉的最强双耐真菌之一,可为微生物或微生物联合植物修复重金属污染土壤提供重要的微生物种质资源.(图8表3参39)
To obtain the microbial resource strains with high heavy metal compound tolerance to remedy heavy mental contaminated soil, soil samples were collected from the tail mining area in Northwest Guangxi. Improved Martin's medium was used to isolate and screen the high-tolerance microorganisms for heavy metal lead and cadmium. The fungus with compound tolerance to lead and cadmium termed K-3 was obtained, whose lead resistance was 1 200 mg/L and cadmium resistance was120 mg/L. The optimum temperature for K-3 was 30 ℃, pH was 7.0, and salt concentration was 3%. The identification of its morphology and molecular biology showed that the sequence consistency of 18 S rDNA between K-3 and the standard strain Uncultured Westerdykella was 99%. By consulting relevant literature and comprehensive inference, it was shown that K-3 was one of the strongest double-tolerance fungi for heavy metal lead and cadmium. It provided precious microbial germplasm resources for remediation of heavy metal contaminated soil using microorganisms or microorganism-associated plants.
To obtain the microbial resource strains with high heavy metal compound tolerance to remedy heavy mental contaminated soil, soil samples were collected from the tail mining area in Northwest Guangxi. Improved Martin's medium was used to isolate and screen the high-tolerance microorganisms for heavy metal lead and cadmium. The fungus with compound tolerance to lead and cadmium termed K-3 was obtained, whose lead resistance was 1 200 mg/L and cadmium resistance was120 mg/L. The optimum temperature for K-3 was 30 ℃, pH was 7.0, and salt concentration was 3%. The identification of its morphology and molecular biology showed that the sequence consistency of 18 S rDNA between K-3 and the standard strain Uncultured Westerdykella was 99%. By consulting relevant literature and comprehensive inference, it was shown that K-3 was one of the strongest double-tolerance fungi for heavy metal lead and cadmium. It provided precious microbial germplasm resources for remediation of heavy metal contaminated soil using microorganisms or microorganism-associated plants.
引文
1陈满怀.土壤-植物系统中的重金属污染[M].北京:科学出版社,1996[Chen MH.Heavy Metal Pollution in Soil-plant Systems[M].Beijing:Science Press,1996]
2 Jin T,Norbert G,Sehlin J.The susceptibility to nephrotoxicity of strepotozotocin-induced diabetic rats sub chronically exposed to cadmium chloride in drinking water[J].Toxicology,1999,142(1):69-75
3 Hart BA,Potts RD.Cadmium adaptation in the lung double-edged sword[J].Toxicology,2001,160(1-3):65-70
4 Berglund M,Akesson A,Bjellerup P,Vahter M.Metal-bone interactions[J].Toxicol Lett,2000,112-1 13:219-225
5 Antonio MT,Cotpas I,Leret ML.Neurochemical changes in newborn rat's brain after gestational cadmium and lead exposure[J].Toxicol Lett,1999,104(1-2):1-9
6陈静生,周家义.中国水环境重金属研究[M].北京:中国环境科学出版社,1992[Chen JS,Zhou JY.Study on Heavy Metals in Chinese Water Environment[M].China Environmental Science Press,1992]
7 Robinsob JB,Tuovinen O.Mechanism of microbial resistance and organomerary compouds physiological biochemical and genetic analyses[J].J Microbiol Rev,1984,48(2):95-124
8钱春香,王明明,许燕波.土壤重金属污染现状及微生物修复技术研究进展[J].东南大学学报(自然科学版),2013,43(3):669-674[Qian CX,Wang MM,Xu YB.Research progress on soil heavy metal pollution and microbial remediation technology[J].J SE Univ(Nat Sci Ed),2013,43(3):669-674]
9 Konopka A,Zakharova T,Bischoff M,Oliver L,Nakatsu C,Turco RF.Microbial biomass and activity in lead-contaminated soil[J].Appl Environ Microb,1999,65(5):2256-2259
10李辉.耐铅、镉微生物的分离鉴定及高效吸附铅菌株的筛选[D].雅安:四川农业大学,2006[Li H.Isolation and identification of leadcadmium resistant microorganisms and screening of highly efficient lead adsorbing strains[D].Ya'an:Sichuan Agricultural University,2006]
11湛方栋,何永美,李元,祖艳群.云南会泽废弃铅锌矿区和非矿区小花南芥根际真菌的耐镉性[J].应用与环境生物学报,2010,16(4):572-576[Zhan FD,He YM,Li Y,Zu YQ.Cadmium tolerance of rhizosphere fungi of arabis alpina in abandoned lead-zinc mining and non-mining area in Huize,Yunnan,China[J].Chin J Appl Environ Biol,2010,16(4):572-576]
12 Zucconi L,Ripa C,Alianiello F,Benedetti A,Onofri S.Lead resistance,sorption and accumulation in a Paecilomyces lilacinus strain[J].Biol Fert Soils,2003,37(1):17-22
13 Mahendra KG,Kiran K,Amita S.Bioremediation of heavy metal polluted environment using resistant bacteria[J].J Environ Res Dev,2014,8(4):883-889
14 Velmurugan N,Hwang G,Sathishkumar M,Choi TK,Lee KJ,Oh BT,Lee YS.Isolation,identification,Pb(Ⅱ)biosorption isotherims and kinetics of a lead adsorbing Penicillium sp.Mrf-1 from South Korean Mine Soil[J].J Environ Sci-China,2010,22(7):1049-1056
15邓坤,胡振光.广西南丹矿产资源及可持续发展探讨[J].矿产与地质,2010,24(6):552-556[Deng K,Hu ZG.Discussion on mineral resources and sustainable development of Nandan,Guangxi[J].Minel Res Geol,2010,24(6):552-556]
16谢华,刘晓海,陈同斌,廖晓勇,阎秀兰,王莉霞.大型古老锡矿影响区土壤和蔬菜重金属含量及其健康风险[J].环境科学,2008,29(12):3503-3507[Xie H,Liu XH,Chen TB,Liao XY,Yan XL,Wang LX.Concentration and health risk of heavy metals in velgetables and soils in region affected by an ancient tin ore[J].Chin J Environ Sci,2008,29(12):3503-3507]
17邹晓锦,仇荣亮,周小勇,郑文晖.大宝山矿区重金属污染对人体健康风险的研究[J].环境科学学报,2008,28(7):1406-1412[Zou XJ,Chou RL,Zhou XY,Zheng WH.Heavy metal contamination and health risk assessment in Dabao Mountain,China[J].Acta Sci Circumst,2008.28(7):1406-1412]
18丁自立.抗耐镉、铬重金属菌株筛选及水稻根际土壤微生态效应研究[D].武汉:华中农业大学,2016[Ding ZL.Screening of resistance to cadmium,chromium strain effects of the rice rhizosphere microecology[D].Wuhan:Huazhong Agricultural University,2016]
19周德庆.微生物学实验教程[M].北京:高等教育出版社,2006[Zhou DQ.Microbiology Experiment Course[M].Beijing:Higher Education Press,2006]
20李丹.耐铅、镉真菌的筛选鉴定及促进芦苇修复污染土壤的效果分析[D].齐齐哈尔:齐齐哈尔大学,2016[Li D.Isolation of leadand cadmium-resistant funges and analysis the effects in promoting the remediation of contaminated soil with Phragmites Australis[D].Qiqihar:Qiqihar University,2016]
21顾晓洁,解卓学,吕嘉东,姜兴颖,赵剑飞,廉琦.白首乌内生细菌分离鉴定及系统发育树分析[J].辽宁中医药大学学报,2018,20(6):52-56[Gu XJ,Xie ZX,Lu JD,Jiang XY,Zhao JF,Lian Q.Isolation and identifition of endophytic bacteria in Cynanchum auriculatum and phylogenetic analysis[J].J Liaoning Univ Trad Chin Med,2018,20(6):52-56]
22乔宏萍,裴梓汝,贺超,李闯,许嘉,张玉荣,崔剑越,王青.抗重金属细菌BCD1的分子鉴定及系统发育分析[J].太原师范学院学报(自然科学版),2018,17(2):90-92+96[Qiao HP,Pei ZR,He C,Li C,Xu J,Zhang YR,Cui JY,Wang Q.Identification and phylogenetic analysis of BCD1 against heavy metal bacteria[J].J Taiyuan Norm Univ(Nat Sci Ed),2018,17(2):90-92+96]
23杨志俊,易忠权,胡学锋,王静,陈琳,崔玉宝.4种常见储藏物螨类ITS2基因片段序列以及系统发育分析[J].中国病原生物学杂志,2018,13(7):729-732+739[Yang ZJ,Yi ZQ,Hu XF,Wang J,Chen L,Cui YB.Sequencing of ITS2 regions in 4 species of storage mites and an analysis of their phylogenetic relationships[J].J Pathogen Biol,2018.13(7):729-732+739]
24陈灿灿,谢英荷,洪坚平,何小凤,李红梅.土壤中耐铅微生物的筛选[J].中国生态农业学报,2007(2):85-87[Chen CC,Xie YH,Hong JP,He XF,Li HM.Screening of lead-resistant microorganisms in soil[J].Chin J Eco-Agric,2007(2):85-87]
25王俊丽,王忠,任建国.耐铅微生物的筛选及其吸附能力的初步研究[J].污染防治技术,2010,23(1):15-17+63[Wang JL,Wang Z,Ren JG.Screening of lead-resistance microorganisms and its preliminary study on adsorption capability[J].Pollut Prev Control Techn,2010,23(1):15-17+63]
26曹德菊.抗铅微生物的筛选及去铅效果的研究[C]//中国农业生态环境保护协会,农业部环境保护科研监测所.第三届全国农业环境科学学术研讨会论文集,北京,2009:4[Cao DJ. Screening of lead-resistance microorganisms and its study on the effect of lead removal[C]//China Agricultural Ecological Environmental Protection Association,Ministry of Agriculture Environmental Protection Research and Monitoring Institute.Proceedings of the Third NationalSymposium on Agricultural Environmental Science,Beijing,2009:4]
27刘或.污泥中耐镉微生物的筛选与鉴定[D].重庆:西南大学.2007[Liu X.Screening and identification of cadmium-resistance microorganisms in sludge[D].Chongqing:Southwest University,2007]
28靳治国.耐铅镉菌株的筛选及其在污染土壤修复中的应用[D].重庆:西南大学,2010[Jin ZG.Screening of lead-cadmium resistance microorganisms and its application in soil remediation[D].Chongqing:Southwest University,2010]
29李同灵,黄寒娟,彭漪,王芳,张硕,祁伟亮,唐赞,刘松青.耐铅微生物的筛选及其吸附性[J].安徽农业大学学报,2018,45(4):696-702[Li TL,Huang HJ,Peng Y,Wang F,Zhang S,Qi WL,Tang Y,Liu SQ.Lead resistance of microbia screening and preliminary determination of its adsorption[J].J Anhui Agric Univ,2018,45(4):696-702]
30曹霞.耐铅镉微生物的筛选及其对污染土壤铅镉化学形态的影响[D].武汉:华中农业大学,2009[Cao X.Screening of lead-cadmium resistance microorganisms and its effect on the chemical form of leadcadmium in contaminated soil[D].Wuhan:Huazhong Agricultural University,2009]
31黄成涛,黄位权,史鼎鼎,徐少慧,黄智刚,蒋代华.一株耐铅镉真菌Q7对香根草吸收累积重金属的效应[J].应用与环境生物学报.2018,24(4):901-907[Huang CT,Huang WQ,Shi DD,Xu SH,Huang ZG,Jiang DH.Effects of a lead and cadmium-resistant fungal strain on the absorption of heavy metals by Vetiveria zizanioides[J].Chin J Appl Environ Biol,2018,24(4):901-907]
32杨雪艳,蒋代华,史进纳,黄位权,黄成涛,李伟,“双耐”细菌-香根草对铅镉复合污染土壤的修复机理[J].应用与环境生物学报,2016.22(5):884-890[Yang XY,Jiang DH,Shi JN,Huang WQ,Huang CT.Li W.Remediation mechanism of"double-resistant"bacteria-Vetiveria zizanioides on Cd and Pb contaminated soil[J].Chin J Appl Environ Biol,2016,22(5):884-890]
33阎晓明,何金柱.重金属污染土壤的微生物修复机理及研究进展[J].安徽农业科学,2002(6):877-879+883[Yan XM,He JZ.Microbiological repair mechanism and research progress of heavy metal contaminated soil[J].J Anhui Agric Sci,2002(6):877-879+883]
34王荣.耐Pb、Cd细菌耐性机理的研究及其对Pb、Cd生物有效性影响的初探[D].武汉:华中农业大学,2010[Wang R.The Mechanism of Pb2+and Cd2+resistance of lead and cadmium-tolerant bacteria and on the influences of them to Pb and Cd bioavailability[D].Wuhan:Huazhong Agricultural University,2010]
35 Robb DA,Pierpoint WS.Metals and micronutrients:uptake and utilization by plants[J].J Appl Ecol 1984:51-69
36 Rajkumar M,Sandhya S,Prasad MNV.Perspectives of plant-associated microbes in heavy metal phytoremediation[J].Biotechnol Adv,2012,30(6):1562-1574
37 Park JH,Bolan N,Megharaj M.Concomitant rock phopsphate dissolution and lead immobilization by phosphate solubilizing bacteria(Enterobacter sp.)[J].J Environ Manage,2011,92(4):1115-1120
38 Urrutia MM.Remobillization of heavy mentals retained as oxyhydroxides or silicates by bacillus subtilis cells[J].Appl Environ Microbiol,1993,59(4):4323-4329
39 Gadd GM.Microbial influence on metal mobility and application for bioremediation[J].Geoder ma,2004,1 22(2/3/4):109-119
2 Jin T,Norbert G,Sehlin J.The susceptibility to nephrotoxicity of strepotozotocin-induced diabetic rats sub chronically exposed to cadmium chloride in drinking water[J].Toxicology,1999,142(1):69-75
3 Hart BA,Potts RD.Cadmium adaptation in the lung double-edged sword[J].Toxicology,2001,160(1-3):65-70
4 Berglund M,Akesson A,Bjellerup P,Vahter M.Metal-bone interactions[J].Toxicol Lett,2000,112-1 13:219-225
5 Antonio MT,Cotpas I,Leret ML.Neurochemical changes in newborn rat's brain after gestational cadmium and lead exposure[J].Toxicol Lett,1999,104(1-2):1-9
6陈静生,周家义.中国水环境重金属研究[M].北京:中国环境科学出版社,1992[Chen JS,Zhou JY.Study on Heavy Metals in Chinese Water Environment[M].China Environmental Science Press,1992]
7 Robinsob JB,Tuovinen O.Mechanism of microbial resistance and organomerary compouds physiological biochemical and genetic analyses[J].J Microbiol Rev,1984,48(2):95-124
8钱春香,王明明,许燕波.土壤重金属污染现状及微生物修复技术研究进展[J].东南大学学报(自然科学版),2013,43(3):669-674[Qian CX,Wang MM,Xu YB.Research progress on soil heavy metal pollution and microbial remediation technology[J].J SE Univ(Nat Sci Ed),2013,43(3):669-674]
9 Konopka A,Zakharova T,Bischoff M,Oliver L,Nakatsu C,Turco RF.Microbial biomass and activity in lead-contaminated soil[J].Appl Environ Microb,1999,65(5):2256-2259
10李辉.耐铅、镉微生物的分离鉴定及高效吸附铅菌株的筛选[D].雅安:四川农业大学,2006[Li H.Isolation and identification of leadcadmium resistant microorganisms and screening of highly efficient lead adsorbing strains[D].Ya'an:Sichuan Agricultural University,2006]
11湛方栋,何永美,李元,祖艳群.云南会泽废弃铅锌矿区和非矿区小花南芥根际真菌的耐镉性[J].应用与环境生物学报,2010,16(4):572-576[Zhan FD,He YM,Li Y,Zu YQ.Cadmium tolerance of rhizosphere fungi of arabis alpina in abandoned lead-zinc mining and non-mining area in Huize,Yunnan,China[J].Chin J Appl Environ Biol,2010,16(4):572-576]
12 Zucconi L,Ripa C,Alianiello F,Benedetti A,Onofri S.Lead resistance,sorption and accumulation in a Paecilomyces lilacinus strain[J].Biol Fert Soils,2003,37(1):17-22
13 Mahendra KG,Kiran K,Amita S.Bioremediation of heavy metal polluted environment using resistant bacteria[J].J Environ Res Dev,2014,8(4):883-889
14 Velmurugan N,Hwang G,Sathishkumar M,Choi TK,Lee KJ,Oh BT,Lee YS.Isolation,identification,Pb(Ⅱ)biosorption isotherims and kinetics of a lead adsorbing Penicillium sp.Mrf-1 from South Korean Mine Soil[J].J Environ Sci-China,2010,22(7):1049-1056
15邓坤,胡振光.广西南丹矿产资源及可持续发展探讨[J].矿产与地质,2010,24(6):552-556[Deng K,Hu ZG.Discussion on mineral resources and sustainable development of Nandan,Guangxi[J].Minel Res Geol,2010,24(6):552-556]
16谢华,刘晓海,陈同斌,廖晓勇,阎秀兰,王莉霞.大型古老锡矿影响区土壤和蔬菜重金属含量及其健康风险[J].环境科学,2008,29(12):3503-3507[Xie H,Liu XH,Chen TB,Liao XY,Yan XL,Wang LX.Concentration and health risk of heavy metals in velgetables and soils in region affected by an ancient tin ore[J].Chin J Environ Sci,2008,29(12):3503-3507]
17邹晓锦,仇荣亮,周小勇,郑文晖.大宝山矿区重金属污染对人体健康风险的研究[J].环境科学学报,2008,28(7):1406-1412[Zou XJ,Chou RL,Zhou XY,Zheng WH.Heavy metal contamination and health risk assessment in Dabao Mountain,China[J].Acta Sci Circumst,2008.28(7):1406-1412]
18丁自立.抗耐镉、铬重金属菌株筛选及水稻根际土壤微生态效应研究[D].武汉:华中农业大学,2016[Ding ZL.Screening of resistance to cadmium,chromium strain effects of the rice rhizosphere microecology[D].Wuhan:Huazhong Agricultural University,2016]
19周德庆.微生物学实验教程[M].北京:高等教育出版社,2006[Zhou DQ.Microbiology Experiment Course[M].Beijing:Higher Education Press,2006]
20李丹.耐铅、镉真菌的筛选鉴定及促进芦苇修复污染土壤的效果分析[D].齐齐哈尔:齐齐哈尔大学,2016[Li D.Isolation of leadand cadmium-resistant funges and analysis the effects in promoting the remediation of contaminated soil with Phragmites Australis[D].Qiqihar:Qiqihar University,2016]
21顾晓洁,解卓学,吕嘉东,姜兴颖,赵剑飞,廉琦.白首乌内生细菌分离鉴定及系统发育树分析[J].辽宁中医药大学学报,2018,20(6):52-56[Gu XJ,Xie ZX,Lu JD,Jiang XY,Zhao JF,Lian Q.Isolation and identifition of endophytic bacteria in Cynanchum auriculatum and phylogenetic analysis[J].J Liaoning Univ Trad Chin Med,2018,20(6):52-56]
22乔宏萍,裴梓汝,贺超,李闯,许嘉,张玉荣,崔剑越,王青.抗重金属细菌BCD1的分子鉴定及系统发育分析[J].太原师范学院学报(自然科学版),2018,17(2):90-92+96[Qiao HP,Pei ZR,He C,Li C,Xu J,Zhang YR,Cui JY,Wang Q.Identification and phylogenetic analysis of BCD1 against heavy metal bacteria[J].J Taiyuan Norm Univ(Nat Sci Ed),2018,17(2):90-92+96]
23杨志俊,易忠权,胡学锋,王静,陈琳,崔玉宝.4种常见储藏物螨类ITS2基因片段序列以及系统发育分析[J].中国病原生物学杂志,2018,13(7):729-732+739[Yang ZJ,Yi ZQ,Hu XF,Wang J,Chen L,Cui YB.Sequencing of ITS2 regions in 4 species of storage mites and an analysis of their phylogenetic relationships[J].J Pathogen Biol,2018.13(7):729-732+739]
24陈灿灿,谢英荷,洪坚平,何小凤,李红梅.土壤中耐铅微生物的筛选[J].中国生态农业学报,2007(2):85-87[Chen CC,Xie YH,Hong JP,He XF,Li HM.Screening of lead-resistant microorganisms in soil[J].Chin J Eco-Agric,2007(2):85-87]
25王俊丽,王忠,任建国.耐铅微生物的筛选及其吸附能力的初步研究[J].污染防治技术,2010,23(1):15-17+63[Wang JL,Wang Z,Ren JG.Screening of lead-resistance microorganisms and its preliminary study on adsorption capability[J].Pollut Prev Control Techn,2010,23(1):15-17+63]
26曹德菊.抗铅微生物的筛选及去铅效果的研究[C]//中国农业生态环境保护协会,农业部环境保护科研监测所.第三届全国农业环境科学学术研讨会论文集,北京,2009:4[Cao DJ. Screening of lead-resistance microorganisms and its study on the effect of lead removal[C]//China Agricultural Ecological Environmental Protection Association,Ministry of Agriculture Environmental Protection Research and Monitoring Institute.Proceedings of the Third NationalSymposium on Agricultural Environmental Science,Beijing,2009:4]
27刘或.污泥中耐镉微生物的筛选与鉴定[D].重庆:西南大学.2007[Liu X.Screening and identification of cadmium-resistance microorganisms in sludge[D].Chongqing:Southwest University,2007]
28靳治国.耐铅镉菌株的筛选及其在污染土壤修复中的应用[D].重庆:西南大学,2010[Jin ZG.Screening of lead-cadmium resistance microorganisms and its application in soil remediation[D].Chongqing:Southwest University,2010]
29李同灵,黄寒娟,彭漪,王芳,张硕,祁伟亮,唐赞,刘松青.耐铅微生物的筛选及其吸附性[J].安徽农业大学学报,2018,45(4):696-702[Li TL,Huang HJ,Peng Y,Wang F,Zhang S,Qi WL,Tang Y,Liu SQ.Lead resistance of microbia screening and preliminary determination of its adsorption[J].J Anhui Agric Univ,2018,45(4):696-702]
30曹霞.耐铅镉微生物的筛选及其对污染土壤铅镉化学形态的影响[D].武汉:华中农业大学,2009[Cao X.Screening of lead-cadmium resistance microorganisms and its effect on the chemical form of leadcadmium in contaminated soil[D].Wuhan:Huazhong Agricultural University,2009]
31黄成涛,黄位权,史鼎鼎,徐少慧,黄智刚,蒋代华.一株耐铅镉真菌Q7对香根草吸收累积重金属的效应[J].应用与环境生物学报.2018,24(4):901-907[Huang CT,Huang WQ,Shi DD,Xu SH,Huang ZG,Jiang DH.Effects of a lead and cadmium-resistant fungal strain on the absorption of heavy metals by Vetiveria zizanioides[J].Chin J Appl Environ Biol,2018,24(4):901-907]
32杨雪艳,蒋代华,史进纳,黄位权,黄成涛,李伟,“双耐”细菌-香根草对铅镉复合污染土壤的修复机理[J].应用与环境生物学报,2016.22(5):884-890[Yang XY,Jiang DH,Shi JN,Huang WQ,Huang CT.Li W.Remediation mechanism of"double-resistant"bacteria-Vetiveria zizanioides on Cd and Pb contaminated soil[J].Chin J Appl Environ Biol,2016,22(5):884-890]
33阎晓明,何金柱.重金属污染土壤的微生物修复机理及研究进展[J].安徽农业科学,2002(6):877-879+883[Yan XM,He JZ.Microbiological repair mechanism and research progress of heavy metal contaminated soil[J].J Anhui Agric Sci,2002(6):877-879+883]
34王荣.耐Pb、Cd细菌耐性机理的研究及其对Pb、Cd生物有效性影响的初探[D].武汉:华中农业大学,2010[Wang R.The Mechanism of Pb2+and Cd2+resistance of lead and cadmium-tolerant bacteria and on the influences of them to Pb and Cd bioavailability[D].Wuhan:Huazhong Agricultural University,2010]
35 Robb DA,Pierpoint WS.Metals and micronutrients:uptake and utilization by plants[J].J Appl Ecol 1984:51-69
36 Rajkumar M,Sandhya S,Prasad MNV.Perspectives of plant-associated microbes in heavy metal phytoremediation[J].Biotechnol Adv,2012,30(6):1562-1574
37 Park JH,Bolan N,Megharaj M.Concomitant rock phopsphate dissolution and lead immobilization by phosphate solubilizing bacteria(Enterobacter sp.)[J].J Environ Manage,2011,92(4):1115-1120
38 Urrutia MM.Remobillization of heavy mentals retained as oxyhydroxides or silicates by bacillus subtilis cells[J].Appl Environ Microbiol,1993,59(4):4323-4329
39 Gadd GM.Microbial influence on metal mobility and application for bioremediation[J].Geoder ma,2004,1 22(2/3/4):109-119