胶束电动色谱技术在蛋白质分离分析中的应用研究进展
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摘要
胶束电动色谱(MEKC)作为一种兼具色谱和电泳分离机理的方法,既具有准固定相与分析物间的相互作用,又可以利用带电分子在电场中迁移行为的差异进行分离。MEKC的这些特点在蛋白质和多肽这类具有高维度数的生物分子的分离中得到了很好的体现。无论是单维分离,还是多维分离,MEKC都显现出在生物大分子分离方面良好的应用前景。近年来,随着CE-MS的不断发展,出现了很多新的MEKC-MS联用技术,进一步显示了MEKC在蛋白质与多肽分离分析中的应用前景。本文首先对胶束电动色谱技术进行了简介,综述了近年来胶束电动色谱技术在蛋白质分离分析中的研究应用进展,并对其发展前景进行了展望。
Micellar electrokinetic chromatography(MEKC) is a hybrid of chromatography and electrophoresis. The separation mechanism is based on the interaction of analytes and pseudo-stationary phase and also the differential mobility in the electric field. Such property of MEKC can be made good use for separations of proteins and peptides, which have high dimensionality. In the past years, either in the form of single dimensional or multi-dimensional separations, MEKC demonstrated its excellent usability in biomolecule separations. Recently, as the development in CE-MS was revisited, there were also new developments in MEKC-MS hyphenation techniques, which further pointed to the usefulness of MEKC in analysis of proteins and peptides. In this article, the recent advances of MEKC applied in separation and analysis of proteins were reviewed and the prospect of this technique was anticipated.
Micellar electrokinetic chromatography(MEKC) is a hybrid of chromatography and electrophoresis. The separation mechanism is based on the interaction of analytes and pseudo-stationary phase and also the differential mobility in the electric field. Such property of MEKC can be made good use for separations of proteins and peptides, which have high dimensionality. In the past years, either in the form of single dimensional or multi-dimensional separations, MEKC demonstrated its excellent usability in biomolecule separations. Recently, as the development in CE-MS was revisited, there were also new developments in MEKC-MS hyphenation techniques, which further pointed to the usefulness of MEKC in analysis of proteins and peptides. In this article, the recent advances of MEKC applied in separation and analysis of proteins were reviewed and the prospect of this technique was anticipated.
引文
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65 Takada Y,Yoshida M,Sakairi M,Koizumi H.Rapid Commun.Mass Spectrom.,1995,9(10):895-896
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2 Terabe S,Otsuka K,Ichikawa K,Tsuchiya A,Ando T.Anal.Chem.,1984,56:111-113
3 Pyell U.Electrokinetic Chromatography:Theory,Instrumentation and Applications.John Wiley & Sons,Ltd,2006:3-32
4 Terabe S.Ann.Rev.Anal.Chem.,2009,2(1):99-120
5 Weber K,Osborn M.J.Biol.Chem.,1969,244(16):4406-4412
6 Lamb D H,Summa L,Lei Q P,Duval G,Adam O.J.Chromatogr.A,2000,894(1):311-318
7 Huang Y,Duan J P,Chen Q R,Chen G N.Electrophoresis,2004,25(7-8):1051-1057
8 Wu Y,Xie J,Wang F,Chen Z L.J.Sep.Sci.,2009,32(3):437-440
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13 Djordjevic N M,Fitzpatrick F,Houdiere F.Electrophoresis,2001,22(12):2398-2402
14 Lin H J,Hsieh K P,Chiou S S,KouH S,Wu S M.J.Pharm.Biomed.Anal.,2016,131:497-502
15 Wang C C,Chen J L,Chen Y L,Cheng H L,Wu S M.Anal.Chim.Acta,2012,744(18):99-104
16 Belal F,El-Din M S,Tolba M,El-Awady M,Elmansi H.Microchem.J.,2016,127:11-21
17 Ren H X,Liu X,Jiang S X.J.Pharm.Biomed.Anal.,2013,78-79:100-104
18 Matczuk M,Foteeva L S,Jarosz M,Galanski M,Keppler B K,Hirokawa T,Timerbaev A R.J.Chromatogr.A,2014,1345:212-218
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22 Akamatsu S,Mitsuhashi T.J.Sep.Sci.,2014,37(3):304-307
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41 Sheng L,Pawliszyn J.Analyst,2002,127(9):1159-1163
42 Yang C,Liu H C,Yang Q,Zhang L Y,Zhang W B,Zhang Y K.Anal.Chem.,2003,75(2):215-218
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44 Quirino J P.Electrophoresis,2011,32(6-7):665-668
45 Yang L Y,Harrata A K,Lee C S.Anal.Chem.,1997,69(10):1820-1826
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47 Ishihama Y,Katayama H,Asakawa N.Anal.Biochem.,2000,287(1):45-54
48 Wang X C,Hou J G,Jann M,Hon Y Y,Shamsi S A.J.Chromatogr.A,2013,1271(1):207-216
49 Nelson W M,Tang Q,Harrata A K,Lee C S.J.Chromatogr.A,1996,749(1-2):219-226
50 Koezuka K,Ozaki H,Matsubara N,Terabe S.J.Chromatogr.B,1997,689(1):3-11
51 Yang L Y,Harrata A K,Lee C S.Anal.Chem.,1997,69(10):1820-1826
52 Somsen G W,Mol R,de Jong G J.J.Chromatogr.A,2010,1217(25):3978-3991
53 Lamoree M H,Tjaden U R,Vandergreef J.J.Chromatogr.A,1995,712(1):219-225
54 Kinde T F,Lopez T D,Dutta D.Anal.Chem.,2015,87(5):2702-2709
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57 Vieira D B,Crowell A M J,Doucette A A.Rapid Commun.Mass Spectrom.,2012,26(5):523-531
58 Takats Z,Wiseman J M,Gologan B,Cooks R G.Science,2004,306(5695):471-473
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60 Myung S,Wiseman J M,Valentine S J,Takats Z,Cooks R G,Clemmer D E.J.Phys.Chem.B,2006,110(10):5045-5051
61 Shin Y S,Drolet B,Mayer R,Dolence K,Basile F.Anal.Chem.,2007,79(9):3514-3518
62 Barbula G K,Safi S,Chingin K,Perry R H,Zare R N.Anal.Chem.,2011,83(6):1955-1959
63 Cody R B,Laramee J A,Durst H D.Anal.Chem.,2005,77(8):2297-2302
64 Chang C L,Xu G G,Bai Y,Zhang C S,Li X J,Li M,Liu Y,Liu H W.Anal.Chem.,2013,85(1):170-176
65 Takada Y,Yoshida M,Sakairi M,Koizumi H.Rapid Commun.Mass Spectrom.,1995,9(10):895-896
66 Mol R,de Jong G J,Somsen G W.Anal.Chem.,2005,77(16):5277-5282
67 He J,Shamsi S A.Electrophoresis,2011,32(10):1164-1175