混合盐胁迫对茶花凤仙种子萌发的影响
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摘要
以茶花凤仙种子为试材,将NaCl、Na_2SO_4、NaHCO_3和Na_2CO_3按1∶1∶1∶1和0∶0∶1∶1两种摩尔配比人工模拟pH值分别为9.56和10.68的盐碱混合胁迫条件,探讨混合盐胁迫对其种子萌发指标的影响,明确种子萌发期对混合盐胁迫的耐受性。结果表明:2种混合盐胁迫处理下,茶花凤仙种子初始萌发时间滞后;与对照相比,种子发芽率呈现"低促高抑"现象,而发芽势、发芽指数和活力指数均随混合盐浓度的升高而逐渐降低。解除混合盐胁迫后,pH值为9.56处理下的茶花凤仙种子仍具有一定的萌发力,而pH值为10.68处理下的种子已完全失活。茶花凤仙种子萌发阶段对2种混合盐胁迫的耐盐适宜浓度分别为68.05 mmol·L~(-1),60.53 mmol·L~(-1),耐盐半致死浓度分别为119.59 mmol·L~(-1),101.18 mmol·L~(-1),耐盐极限浓度分别为202.07 mmol·L~(-1),166.22 mmol·L~(-1)。
Four single salts of NaCl,Na_2SO_4,NaHCO_3 and Na_2CO_3 were combined with ratios of 1∶1∶1∶1 and 0∶0∶1∶1 to artificially simulate the saline-alkaline mixed stresses with pH of 9.56 and 10.68,respectively.It was studied the effects of mixed salt stress on germination index of Impatiens balsamena seeds,and it was determined the tolerance of seeds to mixed salt stress at germination stage.The result showed that the initial germination time was delayed under mixed salt stress.Compared with the control,the germination rate of seeds was promoted by mixed salt stress under lower salinity,and inhibited under higher level.While the germination index,vigor index and germination potential gradually decreased with the increasing salinity.After removing mixed salt stress,Impatiens balsamena seed still had a certain germination capacity under treatment of pH 9.56,while the seeds were completely inactivated under treatment of pH 10.68.The salt-tolerance appropriate concentrations of Impatiens balsamena seed to mixed salt stress of pH 9.56 and 10.68 were 68.05 and 60.53 mmol·L~(-1) respectively,and the salt-tolerance half-lethal concentrations were 119.59 mmol·L~(-1) and 101.18 mmol·L~(-1), and the salt-tolerance limit concentrations were 202.07 mmol·L~(-1) and 166.22 mmol·L~(-1),respectively during seed germination.
Four single salts of NaCl,Na_2SO_4,NaHCO_3 and Na_2CO_3 were combined with ratios of 1∶1∶1∶1 and 0∶0∶1∶1 to artificially simulate the saline-alkaline mixed stresses with pH of 9.56 and 10.68,respectively.It was studied the effects of mixed salt stress on germination index of Impatiens balsamena seeds,and it was determined the tolerance of seeds to mixed salt stress at germination stage.The result showed that the initial germination time was delayed under mixed salt stress.Compared with the control,the germination rate of seeds was promoted by mixed salt stress under lower salinity,and inhibited under higher level.While the germination index,vigor index and germination potential gradually decreased with the increasing salinity.After removing mixed salt stress,Impatiens balsamena seed still had a certain germination capacity under treatment of pH 9.56,while the seeds were completely inactivated under treatment of pH 10.68.The salt-tolerance appropriate concentrations of Impatiens balsamena seed to mixed salt stress of pH 9.56 and 10.68 were 68.05 and 60.53 mmol·L~(-1) respectively,and the salt-tolerance half-lethal concentrations were 119.59 mmol·L~(-1) and 101.18 mmol·L~(-1), and the salt-tolerance limit concentrations were 202.07 mmol·L~(-1) and 166.22 mmol·L~(-1),respectively during seed germination.
引文
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[27]田吉,樊兴路,王林,等.文冠果种子吸水和萌发对盐碱胁迫条件的响应[J].山西农业大学学报(自然科学版),2016,36(9):644-648.
[28]牛通.盐胁迫对凤仙花种子萌发的影响[J].北方园艺,2012(15):86-88.
[29]鱼小军,师尚礼,龙瑞军,等.生态条件下对种子萌发影响研究进展[J].草业科学,2006,23(10):44-49.
[30]李双男,郭慧娟,王晶,等.不同盐碱胁迫对棉花种子萌发的影响[J].种子,2018,37(1):38-45.
[31]王妮妮.混合盐碱胁迫对皂荚种子萌发的影响[J].东北林业大学学报,2017,45(4):14-18,27.
[32]宋庆云,黄圣,吕艳伟.盐碱胁迫对白榆种子萌发和幼苗生长的影响[J].种子,2018,37(7):15-18.
[33]庄华蓉.单盐和混合盐胁迫对沙冬青种子萌发的影响[J].节水灌溉,2015(2):15-20.
[2]Durai M V,Anandalakshmi R,Tamilarasi R.Effect of Water on Seed Germination in Casuarina junghuhniana MIQ and Acacia auriculiformis [J].The Indian Forester,2017,143(3):228-231.
[3]贾永正,张子晗,喻方圆,等.盐胁迫对紫薇种子萌发特性的影响[J].种子,2016,35(10):87-91,94.
[4]Laghmouchi Y,Belmehdi O,Bouyahya A,et al.Effect of temperature,salt stress and pH on seed germination of medicinal plant Origanum compactum[J].Biocatalysis and Agricultural Biotechnology,2017,10:156-160.
[5]黄冬,葛娈,马焕成,等.牛角瓜种子萌发对PEG模拟干旱胁迫的响应[J].种子,2015,34(9):15-19.
[6]吴桐,李翠兰,邵泽强,等.几种花卉植物对土壤中铅富集特征的研究[J].吉林农业大学学报,2012,34(3):305-310,315.
[7]张福平,林小婷.植物生长调节剂对茶花凤仙种子发芽的影响[J].山东农业大学学报(自然科学版),2013,44(3):379-384.
[8]黄志明,刘登凡.茶花凤仙瓶苗开花诱导机理研究[J].江西农业大学学报,2012,34(5):904-908.
[9]孙宁,张磊,赵新海,等.离子注入对凤仙试管苗组织培养和成花的影响[J].华北农学报,2009,24(3):159-161.
[10]杜晓华,孙涌栋,袁少寒,等.两种凤仙花多倍体的诱导[J].西北农业学报,2011,20(7):56-59.
[11]余婷.凤仙花、茶花凤仙和非洲凤仙的耐荫性硏究[D].四川:四川农业大学,2016.
[12]宗宪春,张永乐,司徒琳莉,等.镉胁迫对三种花卉种子萌发的影响[J].湖北农业科学,2012,51(4):744-746.
[13]韦良焕,赵先贵.植物修复镉污染土壤的差异性研究[J].水土保持通报,2009,29(6):99-102.
[14]李翠兰,邵泽强,王玉军,等.水培条件下几种观赏植物对铅的富集特征[J].东北林业大学学报,2011,39(1):49-51.
[15]李娅迪,江辉,沈文华,等.As3+对2种花卉种子萌发和幼苗生长及抗氧化酶的影响[J].山地农业生物学报,2014,33(2):10-13.
[16]马娟霞.锌污染土壤的超积累植物筛选[D].西安:陕西师范大学,2010.
[17]姜云天,张丽娜,薛飞,等.盐胁迫对茶花凤仙种子萌发的影响[J].东北林业大学学报,2014,42(3):37-41.
[18]祖悦.中性盐和碱性盐胁迫对香豌豆和茶花凤仙的影响[D].牡丹江:牡丹江师范学院,2016.
[19]张晓军,祖悦,綦嘉莹,等.混合盐碱胁迫对茶花凤仙种子萌发的影响[J].江苏农业科学,2017,45(22):140-143.
[20]张树文,杨久春,李颖,等.1950 s中期以来东北地区盐碱地时空变化及成因分析[J].自然资源学报,2010,25(3):435-442.
[21]石德成,盛艳敏,赵可夫.复杂盐碱生态条件的人工模拟及其对羊草生长的影响[J].草业学报,1998,7(1):36-41.
[22]李玉梅,姜云天,董雪松.盐胁迫对东北薄荷种子萌发的影响[J].东北林业大学学报,2018,46(2):22-28,34.
[23]姜云天,沈红梅,闫中雪,等.凤仙花种子萌发期对碱性盐胁迫的耐受性评价[J].现代园艺,2014(12):3-4.
[24]王志强,杨哲,杨凡,等.盐碱胁迫对酸枣种子萌发的影响[J].塔里木大学学报,2016,28(3):52-57.
[25]Fang Y J,Li J,Jiang J J,et al.Physiological and epigenetic analyses of Brassica napus seed germination in response to salt stress[J].Acta Physiologiae Plantarum,2017,39(6):128.
[26]李辛,赵文智.雾冰藜(Bassia dasyphylla)种子萌发和幼苗生长对盐碱胁迫的响应[J].中国沙漠,2018,38(2):300-306.
[27]田吉,樊兴路,王林,等.文冠果种子吸水和萌发对盐碱胁迫条件的响应[J].山西农业大学学报(自然科学版),2016,36(9):644-648.
[28]牛通.盐胁迫对凤仙花种子萌发的影响[J].北方园艺,2012(15):86-88.
[29]鱼小军,师尚礼,龙瑞军,等.生态条件下对种子萌发影响研究进展[J].草业科学,2006,23(10):44-49.
[30]李双男,郭慧娟,王晶,等.不同盐碱胁迫对棉花种子萌发的影响[J].种子,2018,37(1):38-45.
[31]王妮妮.混合盐碱胁迫对皂荚种子萌发的影响[J].东北林业大学学报,2017,45(4):14-18,27.
[32]宋庆云,黄圣,吕艳伟.盐碱胁迫对白榆种子萌发和幼苗生长的影响[J].种子,2018,37(7):15-18.
[33]庄华蓉.单盐和混合盐胁迫对沙冬青种子萌发的影响[J].节水灌溉,2015(2):15-20.