油茶皂素和多酚的综合提取工艺研究
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摘要
以油茶枯为研究对象,研究油茶枯中油茶皂素和油茶多酚的综合提取工艺。采用醇-水提取,以得率为考察指标,考察提取时间、提取温度、料液比及乙醇浓度对油茶皂素和多酚得率的影响,并通过正交试验确定最优综合提取工艺。实验结果表明:油茶皂素及多酚的最优综合提取工艺为提取时间2h、提取温度80℃、料液比1∶12、乙醇体积分数60%,在此工艺下,油茶皂素的得率为15.26%,油茶多酚的得率为0.52%(以山奈酚计)。
Study on comprehensive extractions of saponin and polyphenols from the seed cake of Camellia oleifera Abel.was conducted.With the yields as inspection targets,the technology of extracting with ethanol and water was investigated for determining the influences of extracting time,extracting temperature,solid-liquid ratio and ethanol concentration.The optimum comprehensive extraction process was determined by orthogonal test.The experimental results show that extracting time 2 h,extracting temperature 80 ℃,solid-liquid ratio 1∶12 and ethanol concentration 60%.Under the conditions,the extraction yields of saponin and polyphenols were 15.26% and 0.52%(based on kaempferol)respectively.
Study on comprehensive extractions of saponin and polyphenols from the seed cake of Camellia oleifera Abel.was conducted.With the yields as inspection targets,the technology of extracting with ethanol and water was investigated for determining the influences of extracting time,extracting temperature,solid-liquid ratio and ethanol concentration.The optimum comprehensive extraction process was determined by orthogonal test.The experimental results show that extracting time 2 h,extracting temperature 80 ℃,solid-liquid ratio 1∶12 and ethanol concentration 60%.Under the conditions,the extraction yields of saponin and polyphenols were 15.26% and 0.52%(based on kaempferol)respectively.
引文
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[21]马陶陶,张群林,李俊,等.三氯化铝比色法测定中药总黄酮方法的探讨[J].时珍国医国药,2008,19(1):54-56.
[22]黎国庆,许承婷,王立欣,等.油茶枯中两个多酚化合物的提取分离及其抗炎活性研究[J].广西师范大学学报(自然科学版),2018,37(3):68-75.
[2]干丽,李嘉滢,蔡帧艳,等.茶枯饼主要化学成分的研究及综合利用[J].中南药学,2013,11(11):823-826.
[3]吕晓玲,邱松山,孙晓侠,等.油茶总皂苷的抗氧化及清除自由基能力初步研究[J].食品科学,2005,26(11):86-90.
[4]林国荣.油茶饼中茶皂素的分离及生物活性的研究[J].中国粮油学报,2016,31(1):76-79.
[5]陈永忠,马力,刘宗传,等.油茶皂素杀螺效果研究[J].中南林业科技大学学报,2011,31(3):147-150.
[6]LIU X,JIA L,GAO Y,et al.Anti-inflammatory activity of total flavonoids from seeds of Camellia oleifera Abel.[J].Acta Biochimica et Biophysica Sinica,2014,46(10):920-922.
[7]文莉,芦苇,蒋倩,等.茶皂素毒性刺激性试验及抑菌作用研究[J].中国油脂,2011,36(6):58-60.
[8]SAGESAKA-MITANE Y,SUGIURA T,MIWA Y,et al.Effect of tea-leaf saponin on blood pressure of spontaneously hypertensive rats[J].Journal of the Pharmaceutical Society of Japan,1996,116(5):388-395.
[9]董敬娜,敬佩,李光德,等.茶皂素对油菜幼苗生长发育及抗氧化酶系统的影响[J].中国油料作物学报,2011,33(2):185-188.
[10]CHENG Y T,WU S L,HO C Y,et al.Beneficial effects of camellia oil(Camellia oleifera Abel.)on ketoprofeninduced gastrointestinal mucosal damage through upregulation of HO-1and VEGF[J].Journal of Agricultural and Food Chemistry,2014,62(3):642-650.
[11]MURAKAMI T,NAKAMURA J,KAGEURA T,et al.Bioactive saponins and glycosides.XVII.Inhibitory effect on gastric emptying and accelerating effect on gastrointestinal transit of tea saponins:structures of assamsaponins F,G,H,I,and J from the seeds and leaves of the tea plant[J].Chemical and Pharmaceutical Bulletin,2000,48(11):1720-1725.
[12]ZHU X Y,LIN H M,CHEN X,et al.Mechanochemical-assisted extraction and antioxidant activities of kaempferol glycosides from Camellia oleifera Abel.meal[J].Journal of Agricultural and Food Chemistry,2011,59(8):3986-3993.
[13]LI T,ZHANG H,WU C E.Screening of antioxidant and antitumor activities of major ingredients from defatted Camellia oleifera,seeds[J].Food Science&Biotechnology,2014,23(3):873-880.
[14]姜天甲,应铁进,陈秋平,等.油茶籽壳总黄酮的提取及抗氧化研究[J].中国食品学报,2010,10(1):93-99.
[15]周冬虎,申欣,冯晴.绿茶多酚抗紫外线作用机制研究进展[J].现代预防医学,2013,40(16):2991-2995.
[16]YING C J,SUN X F.Ros-related enzyme expressions in endothelial cells regulated by tea polyphenols[J].Biomedical and Environmental Sciences,2004,17(1):33-39.
[17]王婧,高玉堂.茶多酚抗癌的流行病学研究进展[J].肿瘤,2011,31(6):553-557.
[18]ALMAJANO M P,CARBO R,JIMNEZ J A L,et al.Antioxidant and antimicarobial of tea infusinos[J].Food Chemistry,2008,108(1):55-63.
[19]WEBER J M,RUZINDANA-UMUNYANA A,IMBEAULT L,et al.Inhibition of adenovirus infection and adenain by green tea catechins[J].Antiviral Research,2003,58:167-173.
[20]傅春玲,洪奇华,阮辉,等.茶皂素定量测定方法的研究[J].杭州大学学报(自然科学版),1997,24(3):239-242.
[21]马陶陶,张群林,李俊,等.三氯化铝比色法测定中药总黄酮方法的探讨[J].时珍国医国药,2008,19(1):54-56.
[22]黎国庆,许承婷,王立欣,等.油茶枯中两个多酚化合物的提取分离及其抗炎活性研究[J].广西师范大学学报(自然科学版),2018,37(3):68-75.