高效液相色谱法测定驱虫斑鸠菊中异绿原酸A和异绿原酸C含量
|
摘要
目的建立驱虫斑鸠菊中异绿原酸A、异绿原酸C的含量测定方法。方法 Shim-pack VP-ODS C_(18)柱(4.6 mm×250 mm,5μm),甲醇-乙腈0.4%磷酸溶液(13.5∶13.5∶73)为流动相,流速:1 mL·min~(-1),柱温35℃,检测波长323 nm,进样量:10μL。结果异绿原酸A浓度在5.825～69.9μg·mL~(-1)与峰面积呈良好的线性关系,异绿原酸C浓度在5.15～61.80μg·mL~(-1)与峰面积呈良好的线性关系,二者加样回收率分别为98.70%～101.92%(RSD=1.04%,n=9)、95.99%～102.52%(RSD=1.90%,n=9)。结论该方法简便、快速、准确,可用于驱虫斑鸠菊中异绿原酸A、C含量测定及药材质量控制。
Objective To establish the chromatographic conditions of isochlorogenic acid A and isochlorogenic acid C in vernonia anthelmintica. Methods By changing the mobile phase,flow rate,column temperature and other chromatographic conditions,the best chromatographic conditions was we pursued to established. Results The linear relationship between the concentration of isochlorogenic acid A and the peak area was between 5. 825-69. 9 μg · mL~(-1),and the concentration of isochlorogenic acid C,was between 5. 15-61. 80 μg·mL~(-1) and the peak area was good. The sample recovery rates of the two groups were 98.70%-101.92%( RSD = 1.04%,n = 9) 、95.99%-102.52%( RSD = 1.90%,n = 9). Conclusion The method is simple,rapid,accurate and reliable for the determination of isochlorogenic acid A and isochlorogenic acid C in Vernonia anthelmintica and also for the quality control of the raw material.
Objective To establish the chromatographic conditions of isochlorogenic acid A and isochlorogenic acid C in vernonia anthelmintica. Methods By changing the mobile phase,flow rate,column temperature and other chromatographic conditions,the best chromatographic conditions was we pursued to established. Results The linear relationship between the concentration of isochlorogenic acid A and the peak area was between 5. 825-69. 9 μg · mL~(-1),and the concentration of isochlorogenic acid C,was between 5. 15-61. 80 μg·mL~(-1) and the peak area was good. The sample recovery rates of the two groups were 98.70%-101.92%( RSD = 1.04%,n = 9) 、95.99%-102.52%( RSD = 1.90%,n = 9). Conclusion The method is simple,rapid,accurate and reliable for the determination of isochlorogenic acid A and isochlorogenic acid C in Vernonia anthelmintica and also for the quality control of the raw material.
引文
[1]蔡良谟,霍仕霞,闫明,等.驱虫斑鸠菊研究进展[J].中国中医药信息杂志,2011,18(12):110-112.
[2]曾文浩,屈保平,张星平,等.驱虫斑鸠菊治疗白癜风研究进展[J].中医药学报,2016,44(2):96-101.
[3]蔡良谟,霍仕霞,林娟,等.维药驱虫斑鸠菊化学成分研究[J].中成药,2012,34(11):2159-2161.
[4]付瑾,王贝贝,赵爽,等.民族药驱虫斑鸠菊中绿原酸的含量测定[J].中国处方药,2015,13(10):26-27.
[5]林塬,陈静.常用民族药植物中酚酸类化合物的提取、纯化及生物活性研究进展[J].中国民族医药杂志,2014,20(9):39-41.
[6]李柏群,刘骏,陈绍成,等.正交设计优选风芍六君子汤的提取工艺[J].中国药业,2016,25(6):26-29.
[7]周钰,黄国志,梁东辉,等.正交设计进行消淤接骨散的醇提工艺[J].中国组织工程研究与临床康复,2011,15(8):1433-1436.
[8]楼招欢,吕圭源,颜美秋,等.HPLC法测定复方夏枯草片中绿原酸的含量[J].中华中医药学刊,2011,29(6):1250-1252.
[9]计思敏,杨敏,王玄源,等.苦丁茶中异绿原酸的水提工艺研究[J].光明中医,2017,32(1):44-46.
[10]印酬,向红霞,王洪凤,等.贵州不同地区山银花中4种异绿原酸含量测定方法的建立[J].贵州医科大学学报,2016,41(10):1176-1180.
[11]衷林清,何春柳,缪晓冬,等.HPLC同时测定胜红清咽含片中异嗪皮啶、迷迭香酸、绿原酸的含量[J].海峡药学,2016,28(10):48-51.
[12]刘法千,金芳,俞晓兰,等.高效液相色谱法测定雷公藤多苷片中雷公藤红素的含量[J].医药导报,2016,35(增):117-118.
[2]曾文浩,屈保平,张星平,等.驱虫斑鸠菊治疗白癜风研究进展[J].中医药学报,2016,44(2):96-101.
[3]蔡良谟,霍仕霞,林娟,等.维药驱虫斑鸠菊化学成分研究[J].中成药,2012,34(11):2159-2161.
[4]付瑾,王贝贝,赵爽,等.民族药驱虫斑鸠菊中绿原酸的含量测定[J].中国处方药,2015,13(10):26-27.
[5]林塬,陈静.常用民族药植物中酚酸类化合物的提取、纯化及生物活性研究进展[J].中国民族医药杂志,2014,20(9):39-41.
[6]李柏群,刘骏,陈绍成,等.正交设计优选风芍六君子汤的提取工艺[J].中国药业,2016,25(6):26-29.
[7]周钰,黄国志,梁东辉,等.正交设计进行消淤接骨散的醇提工艺[J].中国组织工程研究与临床康复,2011,15(8):1433-1436.
[8]楼招欢,吕圭源,颜美秋,等.HPLC法测定复方夏枯草片中绿原酸的含量[J].中华中医药学刊,2011,29(6):1250-1252.
[9]计思敏,杨敏,王玄源,等.苦丁茶中异绿原酸的水提工艺研究[J].光明中医,2017,32(1):44-46.
[10]印酬,向红霞,王洪凤,等.贵州不同地区山银花中4种异绿原酸含量测定方法的建立[J].贵州医科大学学报,2016,41(10):1176-1180.
[11]衷林清,何春柳,缪晓冬,等.HPLC同时测定胜红清咽含片中异嗪皮啶、迷迭香酸、绿原酸的含量[J].海峡药学,2016,28(10):48-51.
[12]刘法千,金芳,俞晓兰,等.高效液相色谱法测定雷公藤多苷片中雷公藤红素的含量[J].医药导报,2016,35(增):117-118.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |