深绿木霉对山新杨生长及抗叶枯病能力的影响
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摘要
利用深绿木霉(Trichoderma atroviride)ACCC30153菌株不同孢子悬浮液根施山新杨(Populus davidiana×P. alba var. pyramidlis)移栽苗,测定了山新杨根系土壤肥力、生物量、生理酶活性以及抗叶枯病能力,探讨了深绿木霉促进木本植物生长和提高抗叶枯病的能力。结果表明:深绿木霉处理后的山新杨根系土壤p H呈弱酸性,有机质、水解氮和有效磷质量分数明显增加,土壤肥力增强;105、107个·mL~(-1)深绿木霉孢子悬浮液处理60 d的山新杨株高与对照相比分别增长了30.33%和32.91%,地茎、根长、叶片数、鲜质量和干质量都较高;105个·mL~(-1)的深绿木霉孢子悬浮液处理的山新杨抗氧化还原酶(SOD、POD、CAT)和防御酶(PAL和PPO)活性也明显升高;接种叶枯病病原菌后,105个·mL~(-1)的深绿木霉孢子悬浮液处理的山新杨叶片感病面积最小,为山新杨叶片的1.65%。
The rhizosphere of Populus davidiana×P. alba var. pyramidlis( PdPap) transplant seedlings was watered with different spore suspension of Trichoderma atroviride ACCC30153,and the root soil fertility,biomass,physiological enzymes activities and resistance to Alternaria alternata of PdPap was detected for discussing the abilities of promoting the woody plant growth and resistance to A. alternata of T. atroviride. The pH of root soil of PdPap treated with T. atroviride was faintly acid,and the organic matter,hydrolyzable nitrogen and available phosphorus of root soil of PdPap were obviously increased. The stem length of PdPap treated with 1×105 and 1×107 spore·mL~(-1) T. atroviride spore suspension for 60 d were increased by 30.33% and 32.91% compared with the control,respectively,and their stem diameters,root lengths,leaf numbers,fresh masses and dry masses were higher. The antioxidant enzymes( SOD,POD and CAT) and defense enzymes(PAL and PPO) activities of PdPap treated with 1×105 spore·mL~(-1) T. atroviride spore suspension were also obviously increased. The mean lesion area of Pdpap leaves treated with 1×105 spore·mL~(-1) T. atroviride spore suspension was the least after inoculating A. alternata with 1.65% of the leaves.
The rhizosphere of Populus davidiana×P. alba var. pyramidlis( PdPap) transplant seedlings was watered with different spore suspension of Trichoderma atroviride ACCC30153,and the root soil fertility,biomass,physiological enzymes activities and resistance to Alternaria alternata of PdPap was detected for discussing the abilities of promoting the woody plant growth and resistance to A. alternata of T. atroviride. The pH of root soil of PdPap treated with T. atroviride was faintly acid,and the organic matter,hydrolyzable nitrogen and available phosphorus of root soil of PdPap were obviously increased. The stem length of PdPap treated with 1×105 and 1×107 spore·mL~(-1) T. atroviride spore suspension for 60 d were increased by 30.33% and 32.91% compared with the control,respectively,and their stem diameters,root lengths,leaf numbers,fresh masses and dry masses were higher. The antioxidant enzymes( SOD,POD and CAT) and defense enzymes(PAL and PPO) activities of PdPap treated with 1×105 spore·mL~(-1) T. atroviride spore suspension were also obviously increased. The mean lesion area of Pdpap leaves treated with 1×105 spore·mL~(-1) T. atroviride spore suspension was the least after inoculating A. alternata with 1.65% of the leaves.
引文
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[20]龚凤娟,恩特马克·布拉提白,张宇凤,等.具有ACC脱氨酶活性的杜仲内生细菌的分离鉴定及其抗菌活性[J].微生物学通报,2011,38(10):1526-1532.
[21] WANG M,WU C,CHENG Z,et al. Growth and physiological changes in continuously cropped eggplant(Solanum melongena L.)upon relay intercropping with garlic(Allium sativum L.)[J].Front Plant Sci,2015,6:262.doi:10.3389/fpls.2015.00262.
[22] KATO M,HAYAKAWA Y,HYODO H,et al. Wound-induced ethylene synthesis and expression and formation of 1-aminocyclopropane-1-carboxylate(ACC)synthase,ACC oxidase,phenylalanine ammonia-lyase,and peroxidase in wounded mesocarp tissue of Cucurbita maxima[J]. Plant Cell Physiol,2000,41(4):440-447.
[23]张树武,徐秉良,程玲娟,等.深绿木霉对白三叶草促生作用及生理生化特性的影响[J].草业科学,2015,24(2):161-167.
[2] QUALHATO T F,LOPES F A,STEINDORFF A S,et al. Mycoparasitism studies of Trichoderma species against three phytopathogenic fungi:evaluation of antagonism and hydrolytic enzyme production[J]. Biotechnol Lett,2013,35(9):1461-1468.
[3] BENITEZ T,RINCON A M,LIMON M C,et al. Biocontrol mechanisms of Trichoderma Strains[J]. Int Microbiol,2004,7:249-260.
[4] LIU Y,YANG Q. Cloning and heterologous expression of SS10,a subtilisin-like protease displaying antifungal activity from Trichoderma harzianum[J]. FEMS Microbiol Lett,2009,290(1):54-61.
[5] MORN-DIEZ E,HERMOSA R,AMBROSINO P,et al. The ThPG1 endopolygalacturonase is required for the Trichoderma harzianum-plant beneficial interaction[J]. Molecular Plant-Microbe Interactions,2009,22(8):1021-1031.
[6] HOWELL C R,HANSON L E,STIPANOVIC R D,et al. Induction of terpenoid synthesis in cotton roots and control of Rhizoctonia solani by seed treatment with Trichoderma virens[J]. Phytopathology,2000,90(3):248-252.
[7]黄艳青,庄敬华,高增贵,等.木霉菌诱导甜瓜抗枯萎病相关防御反应酶系的研究[J].沈阳农业大学学报,2005,36(5):546-549.
[8] SALAS-MARINA M A,SILVA-FLORES M A,URESTI-RIVERA E E,et al. Colonization of Arabidopsis roots by Trichoderma atroviride promotes growth and enhances systemic disease resistance through jasmonic acid/ethylene and salicylic acid pathways[J].Eur J Plant Pathol,2011,131(1):15-26.
[9] FAN H,LIU Z,ZHANG R,et al. Functional analysis of a subtilisin-like serine protease gene from biocontrol fungus Trichoderma harzianum[J]. Journal of Microbiology,2004,52(2):129-138.
[10]王娜,窦凯,王志英,等.山新杨组培苗生根移栽方法[J].植物研究,2014,34(3):380-384.
[11]王慧.木霉诱导下山新杨组培移栽苗生长特性研究及土壤养分分析[D].哈尔滨:东北林业大学,2014.
[12]李世贵.两种木霉菌对黄瓜枯萎病菌生防作用及根际土壤微生物影响研究[D].北京:中国农业科学院,2010.
[13]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[14]尹大川,杨立宾,邓勋,等.绿木霉对樟子松苗木生长指标及生理生化指标的影响[J].北京林业大学学报,2015,37(1):79-83.
[15]王海滨,王平,陈永华.ACC脱氨酶的作用机理和转基因的应用[J].生物技术通报,2009(4):40-43.
[16]刁桂萍,李松阳,王志英.深绿木霉1-氨基环丙烷-1-羧酸脱氨酶基因的克隆及表达[J].东北林业大学学报,2016,44(8):104-107.
[17]黄盖,高焓,王琛,等. ACC脱氨酶活性菌株ACC 30的分离、鉴定及其促生作用[J].微生物学通报,2013,40(5):812-821.
[18] GLICK B R,TODOROVIC B,CZARNY J,et al. Promotion of plant growth by bacterial ACC deaminase[J]. Critical Reviews in Plant Science,2007,26(5):227-242.
[19]魏素娜,蒋帅,黄锡云,等.旱地小麦根际细菌中产生1-氨基环丙烷-1-羧酸(ACC)脱氨酶菌株的分离和鉴定[J].微生物学通报,2011,38(5):722-728.
[20]龚凤娟,恩特马克·布拉提白,张宇凤,等.具有ACC脱氨酶活性的杜仲内生细菌的分离鉴定及其抗菌活性[J].微生物学通报,2011,38(10):1526-1532.
[21] WANG M,WU C,CHENG Z,et al. Growth and physiological changes in continuously cropped eggplant(Solanum melongena L.)upon relay intercropping with garlic(Allium sativum L.)[J].Front Plant Sci,2015,6:262.doi:10.3389/fpls.2015.00262.
[22] KATO M,HAYAKAWA Y,HYODO H,et al. Wound-induced ethylene synthesis and expression and formation of 1-aminocyclopropane-1-carboxylate(ACC)synthase,ACC oxidase,phenylalanine ammonia-lyase,and peroxidase in wounded mesocarp tissue of Cucurbita maxima[J]. Plant Cell Physiol,2000,41(4):440-447.
[23]张树武,徐秉良,程玲娟,等.深绿木霉对白三叶草促生作用及生理生化特性的影响[J].草业科学,2015,24(2):161-167.