乙肝病毒标记物阳性表型血清标本HBV-DNA的检验分析
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摘要
目的分析乙型病毒性肝炎中乙肝病毒标记物阳性表型血清标本HBV-DNA的临床诊断效果。方法选取2016～2017年本院900多例表面抗原阳性患者中的387例,全部患者均实施乙肝DNA检测,通过酶联免疫吸附试验(ELISA法)、FQ-PCR法定量分析乙肝病毒标记物阳性表型血清标本及其HBVDNA。结果 HBV-DNA阳性例数在疑似乙肝患者血清标本387例为187例,阴性200例,定量最高7.70E+009IU/mL、定量最低2.71E+003IU/mL。相比大三阳组,差异有统计学意义(P <0.05)。结论乙型病毒性肝炎HBVDNA含量经PCR技术检测后,诊断率较为可靠,为患者临床诊治提供了可靠有效途径。
Objective To analyze the clinical diagnostic effect of HBV-DNA in serum samples of positive phenotype of hepatitis B Virus marker in hepatitis B. Methods 387 of more than 900 patients with surface antigen positive in our hospital from 2016 to 2017 were selected.All patients were given DNA testing for hepatitis B.Serum samples of positive phenotype of hepatitis B Virus marker and its HBV-DNA were quantitatively analyzed by enzymelinked immunosorbent assay(ELISA) and FQ-PCR method. Results In 387 serum samples of suspected hepatitis B,the number of HBV-DNA positive cases was 187 and the number of negative cases was 200,with the highest quantitative value of 7.70E+009IU/mL and the lowest quantitative value of 2.71E+003IU/mL.Compared with the great three positive group,the difference was statistically significant(P < 0.05). Conclusion The HBV-DNA content of hepatitis B virus is detected by PCR technology and the diagnostic rate is reliable,which provides a reliable and effective way for clinical diagnosis and treatment.
Objective To analyze the clinical diagnostic effect of HBV-DNA in serum samples of positive phenotype of hepatitis B Virus marker in hepatitis B. Methods 387 of more than 900 patients with surface antigen positive in our hospital from 2016 to 2017 were selected.All patients were given DNA testing for hepatitis B.Serum samples of positive phenotype of hepatitis B Virus marker and its HBV-DNA were quantitatively analyzed by enzymelinked immunosorbent assay(ELISA) and FQ-PCR method. Results In 387 serum samples of suspected hepatitis B,the number of HBV-DNA positive cases was 187 and the number of negative cases was 200,with the highest quantitative value of 7.70E+009IU/mL and the lowest quantitative value of 2.71E+003IU/mL.Compared with the great three positive group,the difference was statistically significant(P < 0.05). Conclusion The HBV-DNA content of hepatitis B virus is detected by PCR technology and the diagnostic rate is reliable,which provides a reliable and effective way for clinical diagnosis and treatment.
引文
[1]曾锦婷,向华国,曾思强,等.乙肝病毒标记物阳性表型血清标本HBV-DNA检验分析的应用价值观察[J].现代诊断与治疗,2016,27(4):613-615.
[2]毛远丽.HBV血清标志物实验室检测的临床意义[J].中华检验医学杂志,2010,33(4):382-384.
[3]温小云,方先松,邱芳.乙肝病毒标记物阳性表型血清标本乙型肝炎病毒(HBV)-DNA临床检验分析的应用价值探讨[J].当代医学,2016,22(3):63-64.
[4]吴琳,唐敏英,张美娜,等.乙肝免疫标志物与乙肝病毒核酸含量与肝功能的关系研究[J].现代生物医学进展,2015,15(7):1241-1244.
[5]李小月,张祖平,李宗光,等.HBV前S1抗原与HBVDNA联合检测的临床意义[J].临床输血与检验,2011,13(1):25-27.
[6]鲍淑华,楼正青,高丽华.乙肝病毒标记物阳性表型血清标本HBV-DNA水平分析[J].中华全科医学,2014,12(6):977-978.
[7]胡淑芬,梁嘉琪,高慧,等.荧光定量聚合酶链反应定量检测乙型肝炎病毒-DNA与酶联免疫吸附试验定性检测乙型肝炎病毒血清标志物的对比分析[J].实用医技杂志,2010,17(11):1012-1014.
[8]黄华泥,张业久,徐五星.HBV-DNA定量检测结果与乙肝两对半模式相关性分析[J].现代中西医结合杂志,2010,19(31):3459-3460.
[9]刘宇静,吴学军,刘晔.荧光定量PCR检测HBV-DNA与化学发光酶免疫法检测乙肝五项的相关性调查[J].标记免疫分析与临床,2014,21(2):197-200.
[10]黄静.荧光定量PCR检测HBV-DNA与ELISA法乙肝免疫学检测的探讨[J].中国医药科学,2013,3(6):110-112.
[11]李小月,张祖平,李宗光,等.HBV前S1抗原与HBVDNA联合检测的临床意义[J].临床输血与检验,2011,13(1):25-27.
[12]温小云,方先松,邱芳.乙肝病毒标记物阳性表型血清标本乙型肝炎病毒(HBV)-DNA临床检验分析的应用价值探讨[J].当代医学,2016,22(3):63-64.
[13]Thompson AJ,Nguyen T,Iser D,et al.Serum hepatitis B surface antigen and hepatitis B e antigen titers:disease phase influences correlation with viral load and intrahepatic hepatitis B virus markers[J].Hepatology,2010,51(6):1933.
[14]蔡兰兰,李振雪,樊冰.乙肝病毒前S1抗原与乙肝血清标志物及HBV-DNA的相关性分析[J].中国医药导报,2010,7(19):91-92.
[2]毛远丽.HBV血清标志物实验室检测的临床意义[J].中华检验医学杂志,2010,33(4):382-384.
[3]温小云,方先松,邱芳.乙肝病毒标记物阳性表型血清标本乙型肝炎病毒(HBV)-DNA临床检验分析的应用价值探讨[J].当代医学,2016,22(3):63-64.
[4]吴琳,唐敏英,张美娜,等.乙肝免疫标志物与乙肝病毒核酸含量与肝功能的关系研究[J].现代生物医学进展,2015,15(7):1241-1244.
[5]李小月,张祖平,李宗光,等.HBV前S1抗原与HBVDNA联合检测的临床意义[J].临床输血与检验,2011,13(1):25-27.
[6]鲍淑华,楼正青,高丽华.乙肝病毒标记物阳性表型血清标本HBV-DNA水平分析[J].中华全科医学,2014,12(6):977-978.
[7]胡淑芬,梁嘉琪,高慧,等.荧光定量聚合酶链反应定量检测乙型肝炎病毒-DNA与酶联免疫吸附试验定性检测乙型肝炎病毒血清标志物的对比分析[J].实用医技杂志,2010,17(11):1012-1014.
[8]黄华泥,张业久,徐五星.HBV-DNA定量检测结果与乙肝两对半模式相关性分析[J].现代中西医结合杂志,2010,19(31):3459-3460.
[9]刘宇静,吴学军,刘晔.荧光定量PCR检测HBV-DNA与化学发光酶免疫法检测乙肝五项的相关性调查[J].标记免疫分析与临床,2014,21(2):197-200.
[10]黄静.荧光定量PCR检测HBV-DNA与ELISA法乙肝免疫学检测的探讨[J].中国医药科学,2013,3(6):110-112.
[11]李小月,张祖平,李宗光,等.HBV前S1抗原与HBVDNA联合检测的临床意义[J].临床输血与检验,2011,13(1):25-27.
[12]温小云,方先松,邱芳.乙肝病毒标记物阳性表型血清标本乙型肝炎病毒(HBV)-DNA临床检验分析的应用价值探讨[J].当代医学,2016,22(3):63-64.
[13]Thompson AJ,Nguyen T,Iser D,et al.Serum hepatitis B surface antigen and hepatitis B e antigen titers:disease phase influences correlation with viral load and intrahepatic hepatitis B virus markers[J].Hepatology,2010,51(6):1933.
[14]蔡兰兰,李振雪,樊冰.乙肝病毒前S1抗原与乙肝血清标志物及HBV-DNA的相关性分析[J].中国医药导报,2010,7(19):91-92.