基于线粒体COI基因序列的蒙古鲌群体遗传结构分析
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摘要
采用线粒体COI基因序列对长江流域湖北段和江苏段蒙古鲌(Chanodichthys mongolicus)不同自然群体的遗传组成和结构进行了分析。结果显示,蒙古鲌种群的遗传多样性水平中等,群体的平均基因多态性为0.664,核苷酸多态性为0.002,不同种群间遗传多样性存在一定的差异。COI基因序列共检测了10种单倍型,其中4种单倍型在蒙古鲌不同种群间共享,其余单倍型为单个种群所特有。分子变异分析表明79.7%的变异发生在种群内。NJ聚类树和单倍型网络图分析均表明蒙古鲌不同种群未形成明显的谱系地理结构。
The genetic variation and structure of the different natural populations of Chanodichthys mongolicus in the middle and lower reaches of the Yangtze River was analyzed by using cytochrome oxidase subunit I(COI) sequencing. It was suggested that those nautral populations of C. mongolicus had the medium level of genetic diversity. Different levels of genetic diversity were found among those wild populations. The haplotype and nucleotide diversity were 0.664 and 0.002, respectively. Ten unique haplotypes were identified among C. mongolicus individuals based on COI sequences. Four haplotypes shared with any two populations of C. mongolicus and each of the other haplotypes belonged to a single population. The molecular variation analysis discolsed the variation within populations was 79.7%. NJ cluster tree and haplotype network analysis all showed that individuals of C. mongolicus were mixed together and did not form obvious phylogeographic structure.
The genetic variation and structure of the different natural populations of Chanodichthys mongolicus in the middle and lower reaches of the Yangtze River was analyzed by using cytochrome oxidase subunit I(COI) sequencing. It was suggested that those nautral populations of C. mongolicus had the medium level of genetic diversity. Different levels of genetic diversity were found among those wild populations. The haplotype and nucleotide diversity were 0.664 and 0.002, respectively. Ten unique haplotypes were identified among C. mongolicus individuals based on COI sequences. Four haplotypes shared with any two populations of C. mongolicus and each of the other haplotypes belonged to a single population. The molecular variation analysis discolsed the variation within populations was 79.7%. NJ cluster tree and haplotype network analysis all showed that individuals of C. mongolicus were mixed together and did not form obvious phylogeographic structure.
引文
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[2]于辉,李华,刘为民,等.梁子湖三种鲌肉质分析[J].水生生物学报,2005,29(5):502-506.
[3]叶少文.长江中游浅水湖泊鱼类群落和系统营养网络模型的研究[D].武汉:中国科学院水生生物研究所,2007.
[4]徐伟,耿龙武,贾钟贺,等.镜泊湖蒙古鲌人工繁育技术初步研究[J].淡水渔业,2009,39(4):63-66.
[5]林明利.长江中下游湖泊蒙古鲌渔业技术及食鱼性鱼类放养对生态系统影响研究[D].武汉:中国科学院水生生物研究所,2012.
[6]彭居俐,王绪祯,王丁,等.基于线粒体COI基因序列的DNA条形码在鲤科鲌属鱼类物种鉴定中的应用[J].水生生物学报,2009,33(2):271-276.
[7]王丹,程庆武,杨镇宇,等.三峡库区鲌属鱼类线粒体COI基因遗传多样性的初步分析[J].水生生物学报,2015,39(5):1054-1058.
[8]王亚龙,李昊成,何勇凤,等.长湖5种鲌摄食器官形态学的比较[J].淡水渔业,2016,46(6):26-32.
[9]冯晓宇,谢楠,冯建彬,等.基于细胞色素b基因序列的鲌亚科鱼类系统发育研究[J].淡水渔业,2009,39(5):23-27.
[10]萨姆布鲁克 J,拉塞尔 D W.分子克隆实验室指南.3版(黄培堂等,译)[M].北京:科学出版社,2002.
[11] Ward R D,Zemlak T S,Innes B H,et al.DNA barcoding Australia’s fish species [J].Phil Trans R Soc B,2005,360(1462):1847-1857.
[12] Thompson J D,Gibson T J,Plewniak F,et al.The Clustal X windows interface:flexible strategies for multiple sequences alignment aided by quality analysis tools [J].Nucl Acids Res,1997,25(24):4876-4882.
[13] Gouy M,Guindon S,Gascuel O.SeaView version 4:a multiplatform graphical user interface for sequence alignment and phylogenetic tree building [J].Mol Biol Evol,2010,27(2):221-224.
[14] Librado P,Rozas J.DnaSP v5:A software for comprehensive analysis of DNA polymorphism data [J].Bioinformatics,2009,25(11):1451-1452.
[15] Excoffier L,Lischer H E L.Arlequin suite ver 3.5:A new series of programs to perform population genetics analyses under Linux and Windows [J].Mol Ecol Resour,2010,10(3):564-567.
[16] Bandelt H J,Forster P,R?hl A.Median-joining networks for inferring intraspecific phylogenies [J].Mol Biol Evol,1999,16(1):37-48.
[17] Tamura K,Stecher G,Peterson D,et al.MEGA6:Molecular evolutionary genetics analysis version 6.0 [J].Mol Biol Evol,2013,30(12):2725-2729.
[18]施立明,宿兵.遗传多样性研究的原理与方法[M].杭州:浙江科学技术出版社,1999.
[19] He D K,Kang Z J,Tao J,et al.Hydrologic connectivity driven natural stream fish assemblages in mountain streams in the Yangtze River basin:implications for stream fish conservation in monsoonal East Asia [J].Hydrobiologia,2016,785(1):185-206.
[20] Grant W S,Bowen B W.Shallow population histories in deep evolutionary lineages of marine fishes:insights from sardines and anchovies and lessons for conservation [J].J Heredity,1998,89(5):415-426.
[21]张清慧,董旭辉,羊向东.湖北梁子湖近百年来环境演变历史及驱动因素分析[J].湖泊科学,2016,28(3):545-553.
[22]王晓华,刘慕凡,张家玉,等.治污工程对武汉东湖生态恢复的研究与探讨[J].环境科学与技术,2002,(2):40-42.
[23]张小立,杨品红,徐黎明,等.翘嘴鲌、蒙古鲌形态特征及年龄与生长的研究[J].内陆水产,2008,33(9):7-10.
[24]李修峰,黄道明,谢文星,等.汉江中游产漂流性卵鱼类产卵场的现状[J].大连水产学院学报,2006,21(2):105-111.
[25]谢文星,黄道明,谢山,等.丹江口水利枢纽兴建后汉江中下游四大家鱼等早期资源及其演变[J].水生态学杂志,2009,2(2):44-49.
[26]林弘都.台湾与中国大陆地区鲤科鱼类之亲缘地理研究[D].台南:国立成功大学,1997.
[27] He S,Liu H,Chen Y,et al.Molecular phylogenetic relationships of Eastern Asian Cyprinidae(Pisces:Cypriniformes) inferred from cytochrome b sequences [J].Sci China Ser:C Life Sci,2004,47(2):130-138.