大肠埃希氏菌在模拟胃肠道环境下耐受性的研究
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摘要
以大肠埃希氏菌为试验菌株,采用活菌计数法,分别探究大肠埃希氏菌在不同酸性、不同胆盐质量浓度环境下的存活情况,并通过模拟胃酸、肠胆盐环境对大肠埃希氏菌的耐受性进行研究.结果表明,酸性、胆盐环境对大肠埃希氏菌的生长具有抑制作用,但胆盐质量浓度的变化对大肠埃希氏菌生长的抑制效果无明显差异.此外,大肠埃希氏菌对模拟胃酸、肠胆盐环境具有良好的耐受性,能在人工肠液以及p H≥4. 5的人工胃液中保持较高的存活率.
Taking escherichia coli as the test strain,its survival in acidic and different concentrations of bile salts was investigated by viable count method,and its tolerance was studied by simulated gastric acid and intestinal bile salt environment. The results showed that the growth of escherichia coli was inhibited by the acidic and bile salt environment,but the growth of escherichia coli was not significantly inhibited by the change of bile salt concentration. Besides,escherichia colihad has good tolerance to simulated gastric acid and intestinal cholate environment,and maintains a high survival rate in artificial intestinal fluid and artificial gastric fluid with a p H value greater than or equal to 4. 5.
Taking escherichia coli as the test strain,its survival in acidic and different concentrations of bile salts was investigated by viable count method,and its tolerance was studied by simulated gastric acid and intestinal bile salt environment. The results showed that the growth of escherichia coli was inhibited by the acidic and bile salt environment,but the growth of escherichia coli was not significantly inhibited by the change of bile salt concentration. Besides,escherichia colihad has good tolerance to simulated gastric acid and intestinal cholate environment,and maintains a high survival rate in artificial intestinal fluid and artificial gastric fluid with a p H value greater than or equal to 4. 5.
引文
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[3]刘骆强,姚艳玲,管佳丽,等. 5种食源性致病菌PCR检测方法的建立[J].食品安全质量检测学报,2019,10(5):1330-1335.
[4]黄文进,尹小燕,莫宗平.产ESBLs大肠埃希菌血流感染的危险因素分析[J].检验医学与临床,2019(9):1297-1298,1312.
[5]房海.大肠埃希氏菌[M].石家庄:河北科学技术出版社,1997.
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