亳桑皮炮制工艺的优化
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摘要
优选亳桑皮的最佳炮制工艺。以桑根酮C含量为指标,对亳桑皮干燥方法和蜜炙方法进行系统研究。分别于60℃烘干、晒干、阴干、霉变后60℃烘干4种不同干燥方法下对亳桑皮质量进行评价,结果表明,于60℃烘干的亳桑皮中桑根酮C含量最高,亳桑皮采收后应进行烘干处理;采用正交试验,确定亳桑皮的最佳炮制工艺:药材与蜜的比例为4∶1,闷润时间为20 min,在120～150℃温度下中火炒制15 min。采用高效液相色谱法测定所炮制的亳桑皮中桑根酮C的含量,桑根酮C含量在0.73～3.65μg范围内与色谱峰面积呈良好的线性关系,相关系数为0.999 9,方法的检出限为0.001 8 mg/mL。测定结果的相对标准偏差为1.47%(n=6),样品加标回收率为96.75%～100.03%。该工艺稳定可行,重现性好,可为亳桑皮的规范化生产提供科学理论依据。
The processing technology of Bozhou Cortex Mori was optimized. The drying method and honey baking method of Bo mulberry bark were systematically studied with the content of sanggunone C as the index. The quality of Bozhou Cortex Mori was evaluated under four different drying methods: drying at 60℃, drying in the sun, drying in the shade and drying at 60℃ after mildew. It was determined that the highest content of sanggenon C in Bozhou Cortex Mori dried at 60℃ was found, the Bozhou Cortex Mori should be dried after harvesting. Orthogonal test was used to determine the optimum processing technology of Bozhou Cortex Mori: the ratio of medicinal materials to honey was 4:1, the moistening time was 20 minutes, and the stir-frying time was 15 minutes at 120–150℃. The content of sanggunone C in Bozhou Cortex Mori was determined by high performance liquid chromatography. There was a good linear relationship between the peak area of chromatogram and the content of sanggunone C in the range of 0.73–3.65 μg, and the correlation coef?cient was 0.999 9, the detection limit was 0.001 8 mg/mL. The relative standard deviation was 1.47%(n=6), and the recoveries were 96.75%–100.03%. The process is stable, feasible and reproducible. It can provide scienti?c theoretical basis for the standardized production of Bomulberry bark.
The processing technology of Bozhou Cortex Mori was optimized. The drying method and honey baking method of Bo mulberry bark were systematically studied with the content of sanggunone C as the index. The quality of Bozhou Cortex Mori was evaluated under four different drying methods: drying at 60℃, drying in the sun, drying in the shade and drying at 60℃ after mildew. It was determined that the highest content of sanggenon C in Bozhou Cortex Mori dried at 60℃ was found, the Bozhou Cortex Mori should be dried after harvesting. Orthogonal test was used to determine the optimum processing technology of Bozhou Cortex Mori: the ratio of medicinal materials to honey was 4:1, the moistening time was 20 minutes, and the stir-frying time was 15 minutes at 120–150℃. The content of sanggunone C in Bozhou Cortex Mori was determined by high performance liquid chromatography. There was a good linear relationship between the peak area of chromatogram and the content of sanggunone C in the range of 0.73–3.65 μg, and the correlation coef?cient was 0.999 9, the detection limit was 0.001 8 mg/mL. The relative standard deviation was 1.47%(n=6), and the recoveries were 96.75%–100.03%. The process is stable, feasible and reproducible. It can provide scienti?c theoretical basis for the standardized production of Bomulberry bark.
引文
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[14]段志涛,高英,周刚.桑白皮药材的质量标准研究[J].中药材,2013,36(4):553-557.
[15]任婧昱,姜文红,曹飞,等.桑白皮与密桑白皮及其相近品种的鉴别研究[J].黑龙江中医药,2015(2):61.
[16]柴尧,彭九嫚,陆杰霖,等.正交试验优选银桑降糖颗粒中桑叶的提取工艺[J].中国实验方剂学杂志,2014,20(24):6-9.
[17]沈小月,宋敏,杭太俊.LC-MS法鉴定桑叶中生物碱类成分[J].药物分析杂志,2016,36(10):1 737-1 744.
[2]徐小飞,陈康,陈燕霞,等.桑白皮蜜炙前后东莨菪内酯含量变化研究[J].广东药学院学报,2011(6):579-581.
[3]周德文,李长敏.桑白皮的药理活性[J].国外医药(植物分册),1997,12(3):155-157.
[4]金玲,居明秋,居明乔.桑白皮历代炮制沿革与功效[J].基层中药杂志,2000(2):39-40.
[5]李群,王瑾,张会敏.正交试验法优选桑白皮蜜炙工艺[J].中草药,2013,44(3):286-290.
[6]李洪玉,孙静芸,吴素香.不同来源桑白皮降压成分桑根酮C含量测定[J].中成药,2002(2):28-30.
[7]杨萍.桑白皮总黄酮研究进展[J].临床合理用药杂志,2018,19:180-181.
[8]陈志永,蒙麦侠,杨媛媛,等.HPLC法同时测定桑白皮中6种活性成分的含量[J].中国药房,2018,29(7):911-913.
[9]董德刚,张秀英,刘小雪.桑白皮有效成分分离纯化的工艺研究[J].中国药房,2015,26(28):3 960-3 963.
[10]张会敏,李群.桑白皮蜜炙前后醋酸乙酯提取物指纹图谱研究[J].时珍国医国药,2014,25(9):2 147-2 148.
[11]张尧,俸婷婷,赵致,等.桑白皮化学成分研究[J].中药材,2013,36(7):1 101-1 103.
[12]郑尚永,刘凯,毛积芳.从桑白皮中提取药物成分桑根酮C及质量分析[J].四川蚕业,2008(1):8-12.
[13]郑尚永,姚海军,毛积芳.桑白皮的鉴别及蜜炙炮制对其主要成分桑根酮C含量的影响[J].四川蚕业,2009(2):16-18,24.
[14]段志涛,高英,周刚.桑白皮药材的质量标准研究[J].中药材,2013,36(4):553-557.
[15]任婧昱,姜文红,曹飞,等.桑白皮与密桑白皮及其相近品种的鉴别研究[J].黑龙江中医药,2015(2):61.
[16]柴尧,彭九嫚,陆杰霖,等.正交试验优选银桑降糖颗粒中桑叶的提取工艺[J].中国实验方剂学杂志,2014,20(24):6-9.
[17]沈小月,宋敏,杭太俊.LC-MS法鉴定桑叶中生物碱类成分[J].药物分析杂志,2016,36(10):1 737-1 744.