2017～2018年山东省H9N2亚型禽流感病毒HA基因的遗传进化分析
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摘要
为了解山东省H9N2亚型禽流感病毒(AIV)的变异情况,采用RT-PCR技术对24株山东分离株的HA基因进行扩增、测序和遗传进化分析。结果显示:分离株HA基因在遗传进化树中均属于欧亚分支的Y280-like分支,与A/DK/HK/Y280/97的HA基因核苷酸序列相似性为89.1%～90.5%。HA裂解位点均只有一个碱性氨基酸(R),符合低致病性AIV的特征。所有毒株234位受体结合位点均为L(亮氨酸),呈现出典型的人流感病毒受体结合特性。另外,分离株均缺失218～220位糖基化位点且新增313～315位糖基化位点。研究表明,分离毒株HA基因在分子水平上发生了变异,要积极应对低致病性禽流感防控面临的新挑战。
To explore the variation of H9N2 subtype avian influenza viruses(AIV), the HA genes of 24 virusesisolated from Shandong province were analyzed. The results showed that the HA genes of the isolates belonged tosub-branch of Y280-like, and the homology between HA genes with A/DK/HK/Y280/97 was 89.1% to 90.5%. No consecutive basic amino acids insertion was found in the HA cleavage sites, showing the characteristic feature oflow-pathogenic AIV. The 234 receptor binding sites of all strains were L(leucine), showing typical human influenzavirus receptor binding properties. In addition, the isolates lacked the 218 to 220 glycosylation sites and added 313 to315 glycosylation sites. The results showed that H9N2 subtype AIV isolate had a tendency to evolve, wich posed a newchallenge to control and prevention for H9N2 avian influenza.
To explore the variation of H9N2 subtype avian influenza viruses(AIV), the HA genes of 24 virusesisolated from Shandong province were analyzed. The results showed that the HA genes of the isolates belonged tosub-branch of Y280-like, and the homology between HA genes with A/DK/HK/Y280/97 was 89.1% to 90.5%. No consecutive basic amino acids insertion was found in the HA cleavage sites, showing the characteristic feature oflow-pathogenic AIV. The 234 receptor binding sites of all strains were L(leucine), showing typical human influenzavirus receptor binding properties. In addition, the isolates lacked the 218 to 220 glycosylation sites and added 313 to315 glycosylation sites. The results showed that H9N2 subtype AIV isolate had a tendency to evolve, wich posed a newchallenge to control and prevention for H9N2 avian influenza.
引文
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[9]李广伟,严专强,廖昌韬,等.两广地区2011~2012年H9N2亚型禽流感病毒的HA基因进化分析[J].中国兽医学报,2014,34(3):461-465.
[10]赵军,柴丽娜,王泽霖,等.1998~2008年中国中部H9N2亚型AIV分离毒株HA基因的进化分析[J].病毒学报,2011,27(2):122-128.
[11]DESHPANDE K L,FRIED V A,ANDO M,et al.Glycosylation affects cleavage of an H5N2 influenza virus hemagglutinin and regulates virulenc[J].Proceedings of the National Academy of Sciences of the United States of America,1987,84(1):36-40.
[12]彭欠欠.H9N2亚型流感病毒HA蛋白糖基化位点变化影响病毒生物学特性的研究[D].扬州:扬州大学,2017.
[13]程冰花,王爱华,刁有祥,等.25株H9N2亚型禽感病毒HA基因的遗传进化分析[J].中国兽医学报,2016,36(6):889-895.
[14]万春和,黄瑜.H9N2亚型福建分离株血凝素蛋白的特征[J].中国兽医学报,2016,36(4):617-622.
[2]陈溥言.兽医传染病学[M].5版.北京:中国农业出版社,2015:80-84.
[3]陈伯伦,张泽纪,陈伟斌.禽流感研究Ⅰ:鸡A型禽流感病毒的分离与血清学初步鉴定[J].中国兽医杂志,1994,20(10):3-5.
[4]WATANABE T,KISO M,FUKUYAMA S,et al.Characterization of H7N9 influenza A viruses isolated from humans[J].Nature,2013,501(7468):551-555.
[5]CHEN H Y,YUAN H,GAO R B,et al.Clinical andepidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection:A descriptive study[J].Lancet,2014,383(9918):714-721.
[6]高彦生.香港发生人的H9N2禽流感[J].动物科学与动物医学,2004,21(1):26.
[7]刁有祥,丁家波.低致病性禽流感病毒对养禽业的危害[J].中国家禽,2006,28(6):1-3.
[8]NAGARAJAN S,RAJUKUMAR K,TOSH C,et al.Isolation and pathotyping of H9N2 avian influenza viruses in Indian poultry[J].Veterinary Microbiology,2009,133(1-2):154-163.
[9]李广伟,严专强,廖昌韬,等.两广地区2011~2012年H9N2亚型禽流感病毒的HA基因进化分析[J].中国兽医学报,2014,34(3):461-465.
[10]赵军,柴丽娜,王泽霖,等.1998~2008年中国中部H9N2亚型AIV分离毒株HA基因的进化分析[J].病毒学报,2011,27(2):122-128.
[11]DESHPANDE K L,FRIED V A,ANDO M,et al.Glycosylation affects cleavage of an H5N2 influenza virus hemagglutinin and regulates virulenc[J].Proceedings of the National Academy of Sciences of the United States of America,1987,84(1):36-40.
[12]彭欠欠.H9N2亚型流感病毒HA蛋白糖基化位点变化影响病毒生物学特性的研究[D].扬州:扬州大学,2017.
[13]程冰花,王爱华,刁有祥,等.25株H9N2亚型禽感病毒HA基因的遗传进化分析[J].中国兽医学报,2016,36(6):889-895.
[14]万春和,黄瑜.H9N2亚型福建分离株血凝素蛋白的特征[J].中国兽医学报,2016,36(4):617-622.