两种抑菌剂对马来眼子菜开放式组织培养的效应
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摘要
沉水植被的种苗快速规模化繁殖一直是困扰沉水植被恢复,以及水体环境生态修复、改善水质的难点之一,应用低成本、高效的开放式组织培养技术可以实现种苗的快速规模化,抑菌剂的筛选是组织培养的关键技术。试验研究了MS培养基中不同浓度的代森锰锌和凯松,在沉水植物马来眼子菜(Potamogeton malaianus)开放式组织培养中的抗菌作用。结果表明:代森锰锌、凯松均可作为开放式植物组织培养的抑菌剂;代森锰锌浓度在0.070~0.100 g·L-1抑菌效果较理想;凯松浓度在0.1%抑菌效果较理想,具有良好的应用价值。
Rapid large-scale propagation of submerged vegetation seedlings has been one of the difficulties in submerged vegetation restoration,ecological restoration and the aquatic environment. According to the low cost and efficient open tissue culture technology application,bacteriostatic agent selection was the key technology. Mancozeb and Kathon with different concentrations were added to MS medium to study their antibacterial action in open tissue culture of Potamogeton malaianus. The results showed that Mancozeb and Kathon showed ideal bacteriostatic effects on Potamogeton malaianus with the concentrations of 0. 07- 0. 10·L- 1and 0. 1%,respectively,which have good application value.
Rapid large-scale propagation of submerged vegetation seedlings has been one of the difficulties in submerged vegetation restoration,ecological restoration and the aquatic environment. According to the low cost and efficient open tissue culture technology application,bacteriostatic agent selection was the key technology. Mancozeb and Kathon with different concentrations were added to MS medium to study their antibacterial action in open tissue culture of Potamogeton malaianus. The results showed that Mancozeb and Kathon showed ideal bacteriostatic effects on Potamogeton malaianus with the concentrations of 0. 07- 0. 10·L- 1and 0. 1%,respectively,which have good application value.
引文
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[15]崔刚,单文修.植物开放式组织培养研究初探[J].山东农业大学学报:自然科学版,2004,35(4):529-533.
[16]解辉,莫廷辉.次氯酸钠在香蕉开放式组织培养中的应用研究[J].热带作物学报,2011,32(5):886-890.
[17]Yanagawa T,Tanaka R,Funai R.Simple micro propagation of ornamentals by direct application of chlorine disinfectants without equipment[J].Plant Biotechnology,2007,76(4):289-293.
[18]Silvio L,T,Juliana MR,Mrcia TT.Influence of Na Cl O on nutrient medium sterilization and on pineapple(Ananas comosus cv.Smooth cayenne)behavior[J].Plant Cell Tissue and Organ Culture,2006,86:375-378.
[19]刘静,孙海伟.混合杀菌剂对植物组培污染防治试验[J].山东林业科技,2004,(5):10-12.
[20]李涛,蒲韵婷.Mn、Cu和Zn在植物生长发育中的生理作用[J].河北农业科学,2008,12(6):12-15.
[21]Candan N,Tarhan L.The correlation between antioxidant enzyme activities and lipid peroxidation levels in Mentha pulegium organs grown in Ca2+,Mg2+,Cu2+,Zn2+and Mn2+stress conditions[J].Plant Science,2003,165(4):769-776.
[22]Rodriguez Estrada AE,Royse DJ.Yield,size and bacterial blotch resistance of Pleurotus eryngii grown on cottonseed hulls/oak sawdust supplemented with manganese,copper and whole ground soybean[J].Bioresource Technology,2007,98(10):1898-1906.
[23]邹炳国,章德宏.凯松CG系列产品的机理、应用及安全与环境综述[J].中国洗涤用品工业,2007,5(5):66-67.
[24]Mutschlera J,Giménez-Arnaua E,Foertsch L.Mechanistic assessment of peptide reactivity assay to predict skin allergens with Kathon CG isothiazolinones[J].Toxicology in Vitro,2009,23(3):439-446.
[2]陈寒.特种水生蔬菜刺苦草组织培养技术研究[D].合肥:安徽农业大学,2009.
[3]赵青华,陈永波.开放式组织培养下魔芋快繁技术研究[J].现代农业科技,2011,(13):112-115.
[4]张饮江,刘晓培.沉水植物对水体净化的研究进展[J].科技导报,2012,30(27):73-77.
[5]田琦,王沛芳.5种沉水植物对富营养化水体的净化能力研究[J].水资源保护,2009,25(1):14-17.
[6]王丹,刘霞.山梨酸钾在红豆杉开放式组织培养中的应用[J].安徽农业科学,2010,38(2):634-635.
[7]黄泰达,莫廷辉.香蕉开放式组织培养中增殖培养基成分优化研究[J].安徽农业科学,2012,40(18):9584-9586.
[8]崔刚,单文修.葡萄开放式组织培养外植体系的建立[J].中国农学通报,2004,20(6):36-38.
[9]王赵玉,张健雄.抑菌剂在开放式植物组织培养中的应用研究[J].北方园艺,2012,(18):125-127.
[10]李洪林,刘锋.沉水植物竹叶眼子菜的组织培养和快速繁殖[J].植物生理学通讯,2007,43(2):329.
[11]李晓燕.抑菌剂抑菌能力比较及其对组培苗生长发育的影响[D].沈阳:辽宁师范大学,2007.
[12]王梅,汤浩茹.抗生素对草莓内生菌的抑制[J].安徽农业科学,2005,33(4):606-607.
[13]王黎波,李晓燕.抗生素在植物组织培养中控制污染的应用[J].辽宁农业科学,2007,(3):69-70.
[14]周俊辉,杨妙贤.在培养基中加入抗生素防止万年青茎段培养污染的研究[J].广西植物,2005,25(3):233-235.
[15]崔刚,单文修.植物开放式组织培养研究初探[J].山东农业大学学报:自然科学版,2004,35(4):529-533.
[16]解辉,莫廷辉.次氯酸钠在香蕉开放式组织培养中的应用研究[J].热带作物学报,2011,32(5):886-890.
[17]Yanagawa T,Tanaka R,Funai R.Simple micro propagation of ornamentals by direct application of chlorine disinfectants without equipment[J].Plant Biotechnology,2007,76(4):289-293.
[18]Silvio L,T,Juliana MR,Mrcia TT.Influence of Na Cl O on nutrient medium sterilization and on pineapple(Ananas comosus cv.Smooth cayenne)behavior[J].Plant Cell Tissue and Organ Culture,2006,86:375-378.
[19]刘静,孙海伟.混合杀菌剂对植物组培污染防治试验[J].山东林业科技,2004,(5):10-12.
[20]李涛,蒲韵婷.Mn、Cu和Zn在植物生长发育中的生理作用[J].河北农业科学,2008,12(6):12-15.
[21]Candan N,Tarhan L.The correlation between antioxidant enzyme activities and lipid peroxidation levels in Mentha pulegium organs grown in Ca2+,Mg2+,Cu2+,Zn2+and Mn2+stress conditions[J].Plant Science,2003,165(4):769-776.
[22]Rodriguez Estrada AE,Royse DJ.Yield,size and bacterial blotch resistance of Pleurotus eryngii grown on cottonseed hulls/oak sawdust supplemented with manganese,copper and whole ground soybean[J].Bioresource Technology,2007,98(10):1898-1906.
[23]邹炳国,章德宏.凯松CG系列产品的机理、应用及安全与环境综述[J].中国洗涤用品工业,2007,5(5):66-67.
[24]Mutschlera J,Giménez-Arnaua E,Foertsch L.Mechanistic assessment of peptide reactivity assay to predict skin allergens with Kathon CG isothiazolinones[J].Toxicology in Vitro,2009,23(3):439-446.
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