牛、羊包虫病及其实验室诊断技术
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摘要
包虫病(echinococcosis)是由寄生于终末宿主(犬、狼、狐狸等)小肠的棘球蚴感染中间宿主(牛、羊、猪、骆驼、人类等)而引起的一种严重的人兽共患寄生虫病。该病在临床表现上缺乏特异性,且多为隐性感染,病原体不易检出,严重危害牧区人民健康和畜牧业发展。对包虫病的病原学特点、流行病学特点、实验室检测和诊断技术作一介绍,以期为该病的预防控制和进一步研究提供参考。
Echinococcosis is a serious zoonotic parasitic disease caused by infection of hydatid cyst, parasitizing in small intestine of definitive hosts such as dog, wolf and fox, in intermediate hosts, including cattle, sheep, pig, camel and human. Echinococcosis lacks specificity in clinical manifestations and most cases are inapparent infections. It is difficult to detect the causative agent of this disease. It seriously endangers the health of the pastoral people and the development of animal husbandry. In this article, the etiology and epidemiology of echinococcosis were characterized, and the research advances in detection and diagnostic techniques of this disease were reviewed, in hoping to provide references for the prevention and control of this disease and its further research.
Echinococcosis is a serious zoonotic parasitic disease caused by infection of hydatid cyst, parasitizing in small intestine of definitive hosts such as dog, wolf and fox, in intermediate hosts, including cattle, sheep, pig, camel and human. Echinococcosis lacks specificity in clinical manifestations and most cases are inapparent infections. It is difficult to detect the causative agent of this disease. It seriously endangers the health of the pastoral people and the development of animal husbandry. In this article, the etiology and epidemiology of echinococcosis were characterized, and the research advances in detection and diagnostic techniques of this disease were reviewed, in hoping to provide references for the prevention and control of this disease and its further research.
引文
[1]于恩庶,徐秉锟.中国人兽共患寄生虫病学[M].福州:福建科学技术出版社,1988:741.
[2]ABU-HASAN N,DARAGMEH M,ADWAN K,et al.Human cystic echinococcosis in the West Bank of Palestine:surgical incidence and seroepidemiological study[J].Parasitology Research,2002,88(2):107-112.
[3]孙艳红,杨亚明.包虫病的治疗研究进展[J].热带病与寄生虫学,2015,13(1):53-58.
[4]贾万忠.我国包虫病的防治现状[J].兽医导刊,2011(6):30-33.
[5]孙雨,王晓英,董洁.牛羊包虫病实验室诊断技术的研究进展[J].中国草食动物科学,2016,36(1):49-52.
[6]孔繁瑶.家畜寄生虫学[M].北京:中国农业大学出版社,1997.
[7]BUISHI I E,NJOROGE E M,BOUAMRA O,et al.Canine echinococcosis in northwest Libya:assessment of coproantigen ELISA,and a survey of infection with analysis of risk-factors[J].Vet Parasitol,2005,130(3/4):223-232.
[8]CARMENA D, SNCHEZ-SERRANO L P, BARBERO-MARTNEZ I.Echinococcus granulosus infection in Spain[J].Zoonoses Public Health,2008,55(3):156-165.
[9]CETINKAYA Z, CIFTCI I H, DEMIREL R, et al. A sero-epidemiologic study on cystic echinococcosis in midwestern region of Turkey[J].Saudi Med J,2005,26(2):350-351.
[10]涛波,张斌,杜宝彪.内蒙古自治区包虫病流行病学调查与防治效果[J].中国人兽共患病学报,2011,27(7):677-678.
[11]李伟,高金亮.包虫病及其诊断技术概述[J].中国卫生检验杂志,2017,27(13):1974-1976.
[12]张旭,古努尔·吐尔逊,米晓云,等.犬细粒棘球绦虫粪抗原夹心ELISA检测方法的建立[J].动物医学进展,2012,33(3):19-23.
[13]张旭,赵莉,张壮志,等.包虫病的诊断方法与预防措施概述[J].草食家畜,2016(6):21-25.
[14]赵莉,张旭,张壮志,等.包虫病诊断技术与预防疫苗的研究进展[J].疾病预防控制通报,2010,59(3):454-459.
[15]LAHMAR S,DEBBEK H,ZHANG L H,et al.Transmission dynamics of the Echinococcus granulosus sheepdog strain(G1 genotype)in camels in Tunisia[J].Vet Parasitol,2004,121(1/2):151-156.
[16]KANDEEL A,AHMED E S,HELMY H,et al. A reprospective hospital study of human cystic echinococcosis in Egypt[J].East Mediterr Health J,2004,10(3):349-357.
[17]庄金秋,梅建国,王艳,等.反向间接血凝试验(RIHA)在动物疾病诊断中的应用进展[J].中国动物检疫,2016,33(6):55-59.
[18]KOSE M,SEVIMLI F K. Prevalence of cystic echinococcosis in slaughtered cattle in Afyonkarahisar[J].Turkiye Parazitol Derg,2008,32(1):27-30.
[19]WASSERMANN M,MACKENSTEDT U,ROMIG T. A loop-mediated isothermal amplification(LAMP)method for the identification of species within the Echinococcus granulosus complex[J].Vet Parasitol,2014,200(1/2):97-103.
[20]NI X, MCMANUS D P, YAN H, et al.Loop-Mediated Isothermal Amplification(LAMP)assay for the identification of Echinococcus multilocularis infections in canine definitive hosts[J].Parasites&Vectors,2014,7(1):254.
[21]LAHMAR S, CHHIDA F B, PTAVY A F,et al. Ultrasonographic screening for cystic echinococcosis in sheep in Tunisia[J].Vet Parasitol,2007,143(1):42-49.
[22]CRAIG P S. Detection of specific circulating antigen,immune complexes and antibodies in human hydatidosis from Turkana(Kenya)and Great Britain, by enzymeimmunoassay[J].Parasite Immunol, 1986, 8(2):171-188.
[2]ABU-HASAN N,DARAGMEH M,ADWAN K,et al.Human cystic echinococcosis in the West Bank of Palestine:surgical incidence and seroepidemiological study[J].Parasitology Research,2002,88(2):107-112.
[3]孙艳红,杨亚明.包虫病的治疗研究进展[J].热带病与寄生虫学,2015,13(1):53-58.
[4]贾万忠.我国包虫病的防治现状[J].兽医导刊,2011(6):30-33.
[5]孙雨,王晓英,董洁.牛羊包虫病实验室诊断技术的研究进展[J].中国草食动物科学,2016,36(1):49-52.
[6]孔繁瑶.家畜寄生虫学[M].北京:中国农业大学出版社,1997.
[7]BUISHI I E,NJOROGE E M,BOUAMRA O,et al.Canine echinococcosis in northwest Libya:assessment of coproantigen ELISA,and a survey of infection with analysis of risk-factors[J].Vet Parasitol,2005,130(3/4):223-232.
[8]CARMENA D, SNCHEZ-SERRANO L P, BARBERO-MARTNEZ I.Echinococcus granulosus infection in Spain[J].Zoonoses Public Health,2008,55(3):156-165.
[9]CETINKAYA Z, CIFTCI I H, DEMIREL R, et al. A sero-epidemiologic study on cystic echinococcosis in midwestern region of Turkey[J].Saudi Med J,2005,26(2):350-351.
[10]涛波,张斌,杜宝彪.内蒙古自治区包虫病流行病学调查与防治效果[J].中国人兽共患病学报,2011,27(7):677-678.
[11]李伟,高金亮.包虫病及其诊断技术概述[J].中国卫生检验杂志,2017,27(13):1974-1976.
[12]张旭,古努尔·吐尔逊,米晓云,等.犬细粒棘球绦虫粪抗原夹心ELISA检测方法的建立[J].动物医学进展,2012,33(3):19-23.
[13]张旭,赵莉,张壮志,等.包虫病的诊断方法与预防措施概述[J].草食家畜,2016(6):21-25.
[14]赵莉,张旭,张壮志,等.包虫病诊断技术与预防疫苗的研究进展[J].疾病预防控制通报,2010,59(3):454-459.
[15]LAHMAR S,DEBBEK H,ZHANG L H,et al.Transmission dynamics of the Echinococcus granulosus sheepdog strain(G1 genotype)in camels in Tunisia[J].Vet Parasitol,2004,121(1/2):151-156.
[16]KANDEEL A,AHMED E S,HELMY H,et al. A reprospective hospital study of human cystic echinococcosis in Egypt[J].East Mediterr Health J,2004,10(3):349-357.
[17]庄金秋,梅建国,王艳,等.反向间接血凝试验(RIHA)在动物疾病诊断中的应用进展[J].中国动物检疫,2016,33(6):55-59.
[18]KOSE M,SEVIMLI F K. Prevalence of cystic echinococcosis in slaughtered cattle in Afyonkarahisar[J].Turkiye Parazitol Derg,2008,32(1):27-30.
[19]WASSERMANN M,MACKENSTEDT U,ROMIG T. A loop-mediated isothermal amplification(LAMP)method for the identification of species within the Echinococcus granulosus complex[J].Vet Parasitol,2014,200(1/2):97-103.
[20]NI X, MCMANUS D P, YAN H, et al.Loop-Mediated Isothermal Amplification(LAMP)assay for the identification of Echinococcus multilocularis infections in canine definitive hosts[J].Parasites&Vectors,2014,7(1):254.
[21]LAHMAR S, CHHIDA F B, PTAVY A F,et al. Ultrasonographic screening for cystic echinococcosis in sheep in Tunisia[J].Vet Parasitol,2007,143(1):42-49.
[22]CRAIG P S. Detection of specific circulating antigen,immune complexes and antibodies in human hydatidosis from Turkana(Kenya)and Great Britain, by enzymeimmunoassay[J].Parasite Immunol, 1986, 8(2):171-188.