p16蛋白在宫颈癌中的表达及其与患者临床特征和预后的关系
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摘要
目的探讨p16蛋白在宫颈癌组织中的表达情况及其与患者临床特征和预后的关系。方法采用免疫组织化学染色法检测116例宫颈癌患者的宫颈癌组织标本和100例子宫肌瘤患者的正常宫颈组织标本中p16蛋白的表达情况,分析宫颈癌组织中p16蛋白的阳性表达情况与宫颈癌患者临床特征和预后的关系。结果宫颈癌组织中p16蛋白的阳性表达率明显高于正常宫颈组织(P﹤0.01)。不同年龄、肿瘤直径、病理类型、宫颈癌分期宫颈癌患者宫颈癌组织中p16蛋白的阳性表达率比较,差异均无统计学意义(P﹥0.05);分化程度为低分化、有淋巴结转移宫颈癌患者宫颈癌组织中p16蛋白的阳性表达率均高于分化程度为中高分化、无淋巴结转移的宫颈癌患者,差异均有统计学意义(P﹤0.05)。Log-rank检验结果显示,p16蛋白阴性表达患者的生存情况优于p16蛋白阳性表达患者(P﹤0.05)。结论低分化、有淋巴结转移宫颈癌患者宫颈癌组织中p16蛋白的阳性表达率较高,且p16蛋白阴性表达宫颈癌患者的生存情况更优。
Objective To investigate the expression of p16 protein in patients with cervical cancer and the relationship with clinical characteristics and prognosis. Method The expression of p16 protein in cervical cancer tissues specimens of 116 cases patients of cervical cancer and normal cervical tissue specimens of 100 cases patients with uterine fibroids were examined by immunohistochemistry. The relationship between the positive expression of p16 protein in cervical cancer tissues and clinical characteristics and prognosis was analyzed. Result The positive expression rate of p16 protein in cervical cancer tissues was significantly higher than that in normal cervical tissues(P<0.01). There was no significant difference in the positive expression rate of p16 protein among patients with cervical cancer at different ages, tumor sizes, pathological types and cervical cancer stages(P>0.05); the positive expression rate of p16 protein in the patients with low-differentiated and positive lymph node metastasis was significantly higher than those with medium-high differentiated and negative lymph node metastasis(P<0.05). Log-rank test showed that the survival conditions of the patients with p16 protein negative expression were better than that of the patients with p16 protein positive expression(P<0.05). Conclusion The expression of p16 protein in cervical cancer tissues was higher in the patients with low-differentiated, lymph node metastasis. The patients with p16 protein negative expression have better survival conditions.
Objective To investigate the expression of p16 protein in patients with cervical cancer and the relationship with clinical characteristics and prognosis. Method The expression of p16 protein in cervical cancer tissues specimens of 116 cases patients of cervical cancer and normal cervical tissue specimens of 100 cases patients with uterine fibroids were examined by immunohistochemistry. The relationship between the positive expression of p16 protein in cervical cancer tissues and clinical characteristics and prognosis was analyzed. Result The positive expression rate of p16 protein in cervical cancer tissues was significantly higher than that in normal cervical tissues(P<0.01). There was no significant difference in the positive expression rate of p16 protein among patients with cervical cancer at different ages, tumor sizes, pathological types and cervical cancer stages(P>0.05); the positive expression rate of p16 protein in the patients with low-differentiated and positive lymph node metastasis was significantly higher than those with medium-high differentiated and negative lymph node metastasis(P<0.05). Log-rank test showed that the survival conditions of the patients with p16 protein negative expression were better than that of the patients with p16 protein positive expression(P<0.05). Conclusion The expression of p16 protein in cervical cancer tissues was higher in the patients with low-differentiated, lymph node metastasis. The patients with p16 protein negative expression have better survival conditions.
引文
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[12]Chen GD,Qian DY,Li ZG,et al.Down-regulation of p16and MGMT promotes the anti-proliferative and pro-apop totic effects of 5-Aza-dC and radiation on cervical cance cells[J].Cell Biochem Funct,2017,35(8):488-496.
[13]McGrath CJ,Garcia R,Trinh TT,et al.Role of p16 testing in cervical cancer screening among HIV-infected women[J].PLoS One,2017,12(10):e0185597.
[14]Huangfu M,Liu L,Xu S,et al.Detecting of p16 Autoanti body as a potential early diagnostic serum biomarker in pa tients with cervical cancer[J].Clin Lab,2016,62(6):11171120.
[15]Wang FL,Yang Y,Liu ZY,et al.Correlation between methylation of the p16 promoter and cervical cancer inci dence[J].Eur Rev Med Pharmacol Sci,2017,21(10)2351-2356.
[16]Liao GD,Kang LN,Chen W,et al.p16 iImmunohisto chemistry interpretation by nonpathologists as an accurat method for diagnosing cervical precancer and cancer[J].Low Genit Tract Dis,2015,19(3):207-211.
[17]Cai S,Han K.Research on expression and importance o p53,p16 and VEGF-C in cervical cancer[J].J Gynecol Ob stet Biol Reprod(Paris),2015,44(7):639-645.
[18]赵娟,马唯,吴林林,等.AEG-1、P16、KI-67蛋白在宫颈癌前病变中的表达与临床意义[J].中国性科学,2016,25(10):35-39.
[19]Kanthiya K,Khunnarong J,Tangjitgamol S,et al.Expres sion of the p16 and Ki67 in cervical squamous intraepithe lial lesions and cancer[J].Asian Pac J Cancer Prev,201617(7):3201-3206.
[20]Xing Y,Wang C,Wu J.Expression of geminin,p16,and Ki-67 in cervical intraepithelial neoplasm and normal tis sues[J].Medicine(Baltimore),2017,96(26):e7302.
[2]Lim AW,Ramirez AJ,Hamilton W,et al.Delays in diagnosis of young females with symptomatic cervical cancer in England:an interview-based study[J].Br J Gen Pract,2014,64(627):e602-e610.
[3]郑金锋,马淑芳,耿明,等.p16和p15及PCNA在子宫颈癌组织中的表达及临床病理意义[J].中华肿瘤防治杂志,2007,14(4):291-293.
[4]樊庆春,李佩玲,王红丽.宫颈癌188例临床特点分析[J].现代医学,2013,41(5):341-343.
[5]Ovestad IT,Dalen I,Hansen E,et al.Clinical value of fully automated p16/Ki-67 dual staining in the triage of HPV-pos itive women in the Norwegian cervical cancer screening program[J].Cancer Cytopathol,2017,125(4):283-291.
[6]Chen CC,Huang LW,Bai CH,et al.Predictive value of p16Ki-67 immunocytochemistry for triage of women with ab normal papanicolaou test in cervical cancer screening:systematic review and meta-analysis[J].Ann Saudi Med2016,36(4):245-251.
[7]杨军,郭睿,黄小钟,等.p16INK4a蛋白在人恶性肿瘤组织中的表达及临床意义[J].临床肿瘤学杂志,2018,23(1)39-43.
[8]黄海萍,叶学正,周琦,等.p53、p16在宫颈上皮内瘤变及宫颈癌中的表达及意义探讨[J].肿瘤预防与治疗,200821(2):136-139.
[9]Jin Y,Guan S,Liu L,et al.Anti-p16 autoantibodies may b a useful biomarker for early diagnosis of esophageal cance[J].Asia Pac J Clin Oncol,2015,11(4):e37-e41.
[10]魏璇,张一兵,杜雪,等.P16蛋白在宫颈上皮内病变及宫颈癌中的表达及意义[J].实用癌症杂志,2014,29(3)276-277.
[11]Ahmad A,Raish M,Shahid M,et al.The synergic effec of HPV infection and epigenetic anomaly of the p16 gen in the development of cervical cancer[J].Cancer Biomark2017,19(4):375-381.
[12]Chen GD,Qian DY,Li ZG,et al.Down-regulation of p16and MGMT promotes the anti-proliferative and pro-apop totic effects of 5-Aza-dC and radiation on cervical cance cells[J].Cell Biochem Funct,2017,35(8):488-496.
[13]McGrath CJ,Garcia R,Trinh TT,et al.Role of p16 testing in cervical cancer screening among HIV-infected women[J].PLoS One,2017,12(10):e0185597.
[14]Huangfu M,Liu L,Xu S,et al.Detecting of p16 Autoanti body as a potential early diagnostic serum biomarker in pa tients with cervical cancer[J].Clin Lab,2016,62(6):11171120.
[15]Wang FL,Yang Y,Liu ZY,et al.Correlation between methylation of the p16 promoter and cervical cancer inci dence[J].Eur Rev Med Pharmacol Sci,2017,21(10)2351-2356.
[16]Liao GD,Kang LN,Chen W,et al.p16 iImmunohisto chemistry interpretation by nonpathologists as an accurat method for diagnosing cervical precancer and cancer[J].Low Genit Tract Dis,2015,19(3):207-211.
[17]Cai S,Han K.Research on expression and importance o p53,p16 and VEGF-C in cervical cancer[J].J Gynecol Ob stet Biol Reprod(Paris),2015,44(7):639-645.
[18]赵娟,马唯,吴林林,等.AEG-1、P16、KI-67蛋白在宫颈癌前病变中的表达与临床意义[J].中国性科学,2016,25(10):35-39.
[19]Kanthiya K,Khunnarong J,Tangjitgamol S,et al.Expres sion of the p16 and Ki67 in cervical squamous intraepithe lial lesions and cancer[J].Asian Pac J Cancer Prev,201617(7):3201-3206.
[20]Xing Y,Wang C,Wu J.Expression of geminin,p16,and Ki-67 in cervical intraepithelial neoplasm and normal tis sues[J].Medicine(Baltimore),2017,96(26):e7302.
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