野艾蒿提取物对金黄色葡萄球菌的抑菌机制研究
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摘要
文章研究野艾蒿提取物对金黄色葡萄球菌(SA)的作用机制。通过野艾蒿提取物作用后SA细胞外碱性磷酸酶(AKP)含量、电导率大小以及DNA和RNA大分子物质含量的变化研究其对SA细胞壁及细胞膜通透性的影响;通过测定SA胞内蛋白含量的变化研究野艾蒿提取物对SA蛋白合成的影响;通过测定苹果酸脱氢酶(MDH)和琥珀酸脱氢酶(SDH)活性研究野艾蒿提取物对SA呼吸代谢的影响;通过扫描电镜观察野艾蒿提取物对SA细胞形态的影响。通过1%的葡聚糖凝胶电泳测定野艾蒿提取物与DNA的相互作用。结果表明,野艾蒿提取物作用后SA胞外AKP含量升高,电导率增加,核酸含量无明显变化,SA胞内蛋白含量降低,MDH和SDH活性降低,细胞形态无明显变化,野艾蒿提取物与SA的DNA发生紧密的结合。由此可知,野艾蒿提取物是通过影响SA细胞壁及细胞膜通透性、胞内蛋白含量、MDH和SDH活性并且与SA的DNA发生结合发挥抑菌作用。
To study the action mechanism of Artemisia argyi extract on Staphylococcus aureus(SA). The effects of Artemisia argyi extract on SA cell wall and cell membrane permeability were studied by changing the content of extracellular alkaline phosphatase(AKP), conductivity and DNA and RNA macromolecule substances after the action of Artemisia argyi extract. The effects of Artemisia argyiextract on SA protein synthesis were studied by measuring the content of intracellular protein in SA. Malic acid dehydrogenase(MDH) and succinate dehydrogenase(SDH) were determined, these activity was used to study the effect of Artemisia argyi extract on respiratory metabolism of SA, and the effect of Artemisia argyi extract on SA cell morphology observed by scanning electron microscopy. The interaction between Artemisia argyi extract and DNA was detected by 1% dextran gel electrophoresis. The results showed that the content of extracellular AKP, conductivity in SA increased, the content of intracellular protein in SA decreased, the activity of MDH and SDH decreased nucleic acid, and the cell morphology did not change significantly after treated with Artemisia argyi extract. Artemisia argyi extract was closely bound to the DNA of SA. It can be concluded that Artemisia argyi extract exerts bacteriostasis by affecting SA cell wall and cell membrane permeability, intracellular protein content, MDH and SDH activity, and binding with SA DNA.
To study the action mechanism of Artemisia argyi extract on Staphylococcus aureus(SA). The effects of Artemisia argyi extract on SA cell wall and cell membrane permeability were studied by changing the content of extracellular alkaline phosphatase(AKP), conductivity and DNA and RNA macromolecule substances after the action of Artemisia argyi extract. The effects of Artemisia argyiextract on SA protein synthesis were studied by measuring the content of intracellular protein in SA. Malic acid dehydrogenase(MDH) and succinate dehydrogenase(SDH) were determined, these activity was used to study the effect of Artemisia argyi extract on respiratory metabolism of SA, and the effect of Artemisia argyi extract on SA cell morphology observed by scanning electron microscopy. The interaction between Artemisia argyi extract and DNA was detected by 1% dextran gel electrophoresis. The results showed that the content of extracellular AKP, conductivity in SA increased, the content of intracellular protein in SA decreased, the activity of MDH and SDH decreased nucleic acid, and the cell morphology did not change significantly after treated with Artemisia argyi extract. Artemisia argyi extract was closely bound to the DNA of SA. It can be concluded that Artemisia argyi extract exerts bacteriostasis by affecting SA cell wall and cell membrane permeability, intracellular protein content, MDH and SDH activity, and binding with SA DNA.
引文
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[19]彭美芳,陈文学,李宇真,等.草果提取物对金黄色葡萄球菌抑菌机制的初探[J].食品工业科技,2013,34(24):79-82.
[2]马麟,韦炳华,胡黎.野艾蒿的化学成分研究[J].广州中医药大学学报,2012,29(04):450-453.
[3]Pandey Bishnu Prasad,Thapa Rupak,Upreti Anil.Chemical composition,antioxidant and antibacterial activities of essential oil and methanol extract of Artemisia vulgaris and Gaultheria fragrantissima collected from Nepal[J].Asian Pacific journal of tropical medicine,2017,10(10).
[4]文荣,周成江,贾彦彬,等.野艾蒿中4种黄酮类化合物对HepG2细胞AMPK和LPIN1表达的影响[J].药物分析杂志,2017,37(01):74-82.
[5]Li Feng,Tao Ya,Qiao Yan,et al.Eupatilin inhibits EGF-induced JB6 cell transformation by targeting PI3K[J].International journal of oncology,2016,49(3).
[6]徐亚军.野生艾蒿乙醇浸提物对枯草芽孢杆菌的抑制作用[J].湖北农业科学,2012,51(09):1786-1788.
[7]徐亚军,赵龙飞.野生艾蒿浸提物对大肠杆菌的抑制作用[J].江苏农业科学,2012,40(04):306-308.
[8]松长青,周本宏,易慧兰,等.石榴皮鞣质的抗菌活性及其对金黄色葡萄球菌的抗菌机制[J].中国医院药学杂志,2016,36(04):259-265.
[9]周志娥,罗秋水,熊建华,等.绿原酸、异绿原酸A对大肠杆菌的抑菌机理[J].食品科技,2014,39(03):228-232.
[10]管敏,张力文,徐致远,等.白毛夏枯草对金黄色葡萄球菌的作用规律及抗菌机理[J].中成药,2017,39(11):2393-2396.
[11]周磊.秦皮素对大肠杆菌的抑菌作用机制[D].沈阳:辽宁师范大学,2013.
[12]徐婷婷.皮寒药对金黄色葡萄球菌的抑制作用及其抑菌机制研究[D].重庆:西南大学,2017.
[13]吴华彰,费鸿君,赵云利,等.萝卜硫素对大肠杆菌抑菌机制的研究[J].四川大学学报(医学版),2012,43(03):386-390.
[14]黄燕飞.黄连水提物对痢疾杆菌的抑菌机制研究[D].合肥:安徽农业大学,2016.
[15]谭潇啸.二氢杨梅素抑制细菌机制的初步探究[D].南昌:南昌大学,2015.
[16]云宝仪,周磊,谢鲲鹏,等.黄芩素抑菌活性及其机制的初步研究[J].药学学报,2012,47(12):1587-1592.
[17]王海涛,王倩,谢明杰.大豆异黄酮对金黄色葡萄球菌的抑菌机制研究[J].中国农业科学,2009,42(07):2586-2591.
[18]盛杰,纪海玉,徐亚超,等.酿酒黄水对枯草芽孢杆菌的抑菌机制研究[J].食品工业科技,2017,38(21):126-129,136.
[19]彭美芳,陈文学,李宇真,等.草果提取物对金黄色葡萄球菌抑菌机制的初探[J].食品工业科技,2013,34(24):79-82.