CHO-S高产量细胞株的新型构建方法及其评价
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摘要
中国仓鼠卵巢(CHO)细胞是工业生产重组蛋白类生物药物的主要宿主细胞之一。重组蛋白工程细胞株的传统构建方法是通过外源基因的随机整合和多步骤的加压筛选获得,这种方法耗时费力。本研究主要介绍了一种新型的悬浮类型CHO细胞(CHO-S)高产细胞株的构建方法,利用Crispr/Cas9介导的定点整合技术,将重组蛋白编码基因快速、准确地整合到CHO-S细胞基因组中的转录活跃区,实现重组蛋白的高产量表达。采用本方法成功构建得到了高表达单克隆抗体细胞株,并验证了细胞产物质量一致性。
Chinese hamster ovary(CHO) cell is one of the most main host cells for the production of recombinant protein biopharmaceuticals. The traditional method for constructing recombinant protein engineering cell lines is to insert exogenous gene randomly and pressure screening with a multi-step process. However, this method is time-consuming. Moreover, due to the uncontrollability of insertion sites in random integration, protein productivity of the selected clones may diminish over time, causing the instability of the cell lines, which is known as "position effect".Site-specific integration is a promising alternative to develop recombinant CHO cell lines. In this study, we described a new method to construct the high-expression CHO-S cells though inserting the recombinant protein coding gene into the transcription hot spot with the method of Crispr/Cas9 mediated site-specific integration and achieved the high expression of recombinant protein.
Chinese hamster ovary(CHO) cell is one of the most main host cells for the production of recombinant protein biopharmaceuticals. The traditional method for constructing recombinant protein engineering cell lines is to insert exogenous gene randomly and pressure screening with a multi-step process. However, this method is time-consuming. Moreover, due to the uncontrollability of insertion sites in random integration, protein productivity of the selected clones may diminish over time, causing the instability of the cell lines, which is known as "position effect".Site-specific integration is a promising alternative to develop recombinant CHO cell lines. In this study, we described a new method to construct the high-expression CHO-S cells though inserting the recombinant protein coding gene into the transcription hot spot with the method of Crispr/Cas9 mediated site-specific integration and achieved the high expression of recombinant protein.
引文
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[10]HUANG Y,LI Y,WANG Y G,et al.An efficient and targeted gene integration system for high-level antibody expression[J].J Immunol Methods,2007,322(1/2):28-39.
[11]LOMBARDO A,CESANA D,GENOVESE P,et al.Site-specific integration and tailoring of cassette design for sustainable gene transfer[J].Nat Methods,2011,8(10):861-869.
[12]SUN T,LI C D,HAN L,et al.Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody[J].Eng Life Sci,2015,15(6):660-666.
[13]ZONG H F,HAN L,DING K,et al.Producing defucosylated antibodies with enhanced in vitro antibodydependent cellular cytotoxicity via FUT8 knockout CHO-Scells[J].Eng Life Sci,2017,17(7):801-808.
[14]KAWABE Y,KOMATSU S,KOMATSU S,et al.Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems[J].J Biosci Bioeng,2018,12(5):599-605.
[15]DOENCH J G,FUSI N,SULLENDER M,et al.Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9[J].Nat Biotechnol,2016,34(2):184-191.
[16]KLEINSTIVER B P,PATTANAYAK V,PREW M S,et al.High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects[J].Nature,2016,529(7587):490-495.
[2]ZHU J W.Mammalian cell protein ex pressio n f or biopharmaceutical production[J].Biotechnol Adv,2012,30(5):1158-1170.
[3]HACKER D L,BALASUBRAMANIAN S,et al.Recombinant protein production from stable mammalian cell lines and pools[J].Curr Opin Struct Biol,2016,38:129-136.
[4]KAWABE Y,SHIMOMURA T,HUANG S H,et al.Targeted transgene insertion into the CHO cell genome using Cre recombinase-incorporating integrase-defective retroviral vectors[J].Biotechnol Bioeng,2016,113(7):1600-1610.
[5]INNISS M C,BANDARA K,JUSIAK B,et al.A novel Bxb1 integrase RMCE system for high fidelity site-specific integration of mAb expression cassette in CHO Cells[J].Biotechnol Bioeng,2017,114(8):1837-1846.
[6]朱建伟,王佳贤,赵梦琳,等.Crispr/Cas9技术在CHO细胞中进行基因定点敲入的应用[J].中国医药工业杂志,2017,48(1):33-37.
[7]GUPTA S K,SHUKLA P.Gene editing for cell engineering:trends and applications[J].Crit Rev Biotechnol,2017,37(5):672-684.
[8]RITTER A,VOEDISCH B,WIENBERG J,et al.Deletion of a telomeric region on chromosome 8 correlates with higher productivity and stability of CHO cell lines[J].Biotechnol Bioeng,2016,113(5):1084-1093.
[9]LEE J S,KALLEHAUGE T B,PEDERSEN L E,et al.Sitespecific integration in CHO cells mediated by CRISPR/Cas9and homology-directed DNA repair pathway[J].Sci Rep,2015,5(8572):1-11.
[10]HUANG Y,LI Y,WANG Y G,et al.An efficient and targeted gene integration system for high-level antibody expression[J].J Immunol Methods,2007,322(1/2):28-39.
[11]LOMBARDO A,CESANA D,GENOVESE P,et al.Site-specific integration and tailoring of cassette design for sustainable gene transfer[J].Nat Methods,2011,8(10):861-869.
[12]SUN T,LI C D,HAN L,et al.Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody[J].Eng Life Sci,2015,15(6):660-666.
[13]ZONG H F,HAN L,DING K,et al.Producing defucosylated antibodies with enhanced in vitro antibodydependent cellular cytotoxicity via FUT8 knockout CHO-Scells[J].Eng Life Sci,2017,17(7):801-808.
[14]KAWABE Y,KOMATSU S,KOMATSU S,et al.Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems[J].J Biosci Bioeng,2018,12(5):599-605.
[15]DOENCH J G,FUSI N,SULLENDER M,et al.Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9[J].Nat Biotechnol,2016,34(2):184-191.
[16]KLEINSTIVER B P,PATTANAYAK V,PREW M S,et al.High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects[J].Nature,2016,529(7587):490-495.