大孔吸附树脂纯化常春藤皂苷C工艺研究
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摘要
通过静态吸附试验比较7种树脂对常春藤皂苷C的吸附与解吸,筛选出效果最佳树脂,通过动态吸附试验对最佳树脂的上样p H、上样体积、洗脱液浓度、洗脱体积、洗脱流速进行优化。结果表明:HPD-100树脂对常春藤皂苷C的吸附与解吸性能最好,HPD-100树脂对常春藤皂苷C纯化的最佳条件为:上样体积为6BV,洗脱液乙醇浓度为80%,洗脱体积为7 BV,洗脱流速为1 BV/h。
The best macroreticular resin was selected by comparing adsorption and elution performance of seven kinds of macroreticular resin through static adsorption experiment. And purifying process parameters such as the p H and volume of sample loading,concentration of washing solution,elution volume,and elution flow rate were optimized through dynamic state absorbance experiment. The results showed that HPD- 100 macroreticular resin had the best adsorption and elution performance. The optimum conditions of purification of hederacoside C were sample loading volume on 6 BV,elution volume on 7 BV with 80% ethanol,elution velocity was 1 BV / h.
The best macroreticular resin was selected by comparing adsorption and elution performance of seven kinds of macroreticular resin through static adsorption experiment. And purifying process parameters such as the p H and volume of sample loading,concentration of washing solution,elution volume,and elution flow rate were optimized through dynamic state absorbance experiment. The results showed that HPD- 100 macroreticular resin had the best adsorption and elution performance. The optimum conditions of purification of hederacoside C were sample loading volume on 6 BV,elution volume on 7 BV with 80% ethanol,elution velocity was 1 BV / h.
引文
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[5]王跃生,王洋.大孔吸附树脂研究进展[J].中国中药杂志,2006,31(12):961-965.
[6]张新宇,张笛,王琳,等.绞股蓝皂苷提取纯化工艺研究进展[J].食品工业科技,2014,35(18):378-382.
[7]李淑珍,李进,杨志江,等.大孔树脂分离纯化黑果枸杞总黄酮的研究[J].食品科学,2009(1):19-24.
[8]李春美,钟朝辉,窦宏亮,等.大孔树脂分离纯化柚皮黄酮的研究[J].农业工程学报,2006,22(3):153-157.
[9]崔翰明,张秋燕,林海,等.三七总皂苷的大孔吸附树脂纯化工艺和质量分析研究[J].中草药,2012,43(11):2177-2182.
[10]景怡,景荣琴,任远,等.AB-8大孔吸附树脂分离纯化玉米须中总黄酮的研究[J].中医药学报,2010,38(1):75-78.
[11]童星.中华常春藤中皂苷类成分和挥发油分离分析研究[D].长沙:中南大学,2007.
[12]董微,郜玉钢,何忠梅,等.人参中人参皂苷提取分离研究进展[J].食品工业科技,2014,35(17):366-369.
[13]曾建刚,孙化鹏,阮琴妹,等.星点设计-效应面法优化常春藤皂苷C提取工艺[J].湖南农业科学,2015(2):91-94,98.
[14]李颖畅,郑凤娥,孟宪军.大孔树脂纯化蓝莓果中花色苷的研究[J].食品与生物技术学报,2009,28(4):496-500.
[15]苏东晓,张瑞芬,张名位,等.荔枝果肉酚类物质大孔树脂分离纯化工艺优化[J].中国农业科学,2014,47(14):2897-2906.