鸡源致病性大肠杆菌的分离鉴定及药敏试验
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摘要
为了解鸡源致病性大肠杆菌的生物学特性和耐药性,试验通过采集病料、细菌分离培养、16S rRNA基因鉴定、致病性试验、药敏试验等方法。结果表明:分离出的20株细菌的培养特征、镜检特征均符合大肠杆菌特征;16S rRNA基因序列经BLAST比对与大肠杆菌同源性达99.9%～100%,确定20株分离菌为鸡源大肠杆菌;20株大肠杆菌的致病力为40%～100%;10株鸡源致病性大肠杆菌对15种抗菌药物均呈现多重耐药,耐药率为33.3%～93.3%,耐药情况较严重和复杂。
To understand the biological traits and drug resistance of pathogenic Escherichia coli from chicken, we conducted the study by collecting disease materials, bacterial isolation and culture, 16 S rRNA gene identification,pathogenicity test, drug sensitivity test and other tests. The results show that the culture and microscopic traits of the20 isolates are consistent with the traits of E. coli; the 16 S rRNA gene sequence was 99.9%-100% homologous to E.coli by BLAST, and 20 isolates were identified as chicken-derived Escherichia coli; 20 strains of Escherichia coli have a pathogenicity of 40% to 100%; 10 strains of chicken-derived pathogenic Escherichia coli have multi-drug resistance to 15 antimicrobial agents, and the resistance rate is 33.3%-93.3%. The drug-resistant situation is serious and complicated.
To understand the biological traits and drug resistance of pathogenic Escherichia coli from chicken, we conducted the study by collecting disease materials, bacterial isolation and culture, 16 S rRNA gene identification,pathogenicity test, drug sensitivity test and other tests. The results show that the culture and microscopic traits of the20 isolates are consistent with the traits of E. coli; the 16 S rRNA gene sequence was 99.9%-100% homologous to E.coli by BLAST, and 20 isolates were identified as chicken-derived Escherichia coli; 20 strains of Escherichia coli have a pathogenicity of 40% to 100%; 10 strains of chicken-derived pathogenic Escherichia coli have multi-drug resistance to 15 antimicrobial agents, and the resistance rate is 33.3%-93.3%. The drug-resistant situation is serious and complicated.
引文
[1]张俊丰,黄东璋.鸡肠道大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2013,40(11):179-183.
[2]于阿芳.鸡致病性大肠杆菌的药敏试验、血清型鉴定及耐药基因的PCR检测[J].泰安:山东农业大学,2009.
[3]王宇,王永芬.鸡大肠杆菌病的流行病学研究[J].信阳师范学院学报,2007,20(3):317-340.
[4] MURRAY B E.New aspects of antimicrobial resistence and resulting the therapeutic dilemmas[J].Infect Dis,1991,163:1185.
[5]严玉霖,雷华.鹅大肠杆菌的分离及药敏试验[M].中国畜牧兽医,2011,38(10):160-162.
[6]秦双双,张红.鸭源致病性大肠杆菌的分离鉴定及致病性观察[J].湖北农业科学,2011,50(1):119-121.
[7]冯方周,张晓冬.辽西地区致病性鸡大肠杆菌的分离鉴定及16SrRNA序列分析[J].黑龙江畜牧兽医,2017(15):191-195.
[8]张轶,王春光.猪源大肠杆菌的分离、鉴定及耐药性分析[J].中国农学通报,2005,21(12):23-30.
[9]刘玉涛,吴学敏.福建省鸡源大肠杆菌的分离鉴定及药敏试验[M].中国农学通报,2014,30(2):65-68.
[10]郝智慧,肖希龙.不同区域鸡大肠杆菌对抗菌药的耐药耐药性比较[J].中国收兽医科学,2009,39(1):84-88.
[11]常宏伟,赵俊.17株产H2S致泄大肠埃希菌的微生物学研究大肠杆菌的分离鉴定及药敏试验[J].中华疾病控制杂志,2009,(3):320-323.
[12]刁有祥,李久芹.山东省鸡大肠杆菌的分离鉴定[J].中国预防兽医学报,2002,24(1):21-23.
[13]徐海花,牛仲相.鸡源致病性大肠杆菌地方株的分离与鉴定[J].山东家禽,2004:7-10.
[14]孙协军,陈琴琴.六安市鸡大肠杆菌的分离鉴定及药敏试验[J].畜牧与饲料科学,2008,5:43-45.
[15]师福山,赵德明.禽大肠杆菌的分离与16SrRNA的鉴定[J].中国畜牧兽医,2009,36(2):111-113.
[2]于阿芳.鸡致病性大肠杆菌的药敏试验、血清型鉴定及耐药基因的PCR检测[J].泰安:山东农业大学,2009.
[3]王宇,王永芬.鸡大肠杆菌病的流行病学研究[J].信阳师范学院学报,2007,20(3):317-340.
[4] MURRAY B E.New aspects of antimicrobial resistence and resulting the therapeutic dilemmas[J].Infect Dis,1991,163:1185.
[5]严玉霖,雷华.鹅大肠杆菌的分离及药敏试验[M].中国畜牧兽医,2011,38(10):160-162.
[6]秦双双,张红.鸭源致病性大肠杆菌的分离鉴定及致病性观察[J].湖北农业科学,2011,50(1):119-121.
[7]冯方周,张晓冬.辽西地区致病性鸡大肠杆菌的分离鉴定及16SrRNA序列分析[J].黑龙江畜牧兽医,2017(15):191-195.
[8]张轶,王春光.猪源大肠杆菌的分离、鉴定及耐药性分析[J].中国农学通报,2005,21(12):23-30.
[9]刘玉涛,吴学敏.福建省鸡源大肠杆菌的分离鉴定及药敏试验[M].中国农学通报,2014,30(2):65-68.
[10]郝智慧,肖希龙.不同区域鸡大肠杆菌对抗菌药的耐药耐药性比较[J].中国收兽医科学,2009,39(1):84-88.
[11]常宏伟,赵俊.17株产H2S致泄大肠埃希菌的微生物学研究大肠杆菌的分离鉴定及药敏试验[J].中华疾病控制杂志,2009,(3):320-323.
[12]刁有祥,李久芹.山东省鸡大肠杆菌的分离鉴定[J].中国预防兽医学报,2002,24(1):21-23.
[13]徐海花,牛仲相.鸡源致病性大肠杆菌地方株的分离与鉴定[J].山东家禽,2004:7-10.
[14]孙协军,陈琴琴.六安市鸡大肠杆菌的分离鉴定及药敏试验[J].畜牧与饲料科学,2008,5:43-45.
[15]师福山,赵德明.禽大肠杆菌的分离与16SrRNA的鉴定[J].中国畜牧兽医,2009,36(2):111-113.