不同水平饲料镉对罗非鱼肝胰脏抗氧化能力影响的研究
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摘要
为研究不同水平饲料镉对罗非鱼肝胰脏抗氧化能力的影响。将300尾罗非鱼随机分为饲料镉添加水平为0、50、100、200、400mg/kg的5个处理,每个处理4个重复。养殖8周后,测定肝胰脏有关抗氧化能力指标。结果表明:随镉水平升高,罗非鱼肝胰脏MDA水平升高,200mg/kg添加水平组开始显著高于对照组(P<0.05);T-AOC水平逐渐降低,50mg/kg时即可显著降低(P<0.05);CAT和SOD活性均先升高后下降,其活性分别在镉添加水平为50、200mg/kg时最高,并显著高于对照组(P<0.05)。表明饲料中添加不同水平镉对罗非鱼肝胰脏抗氧化指标造成不同影响,饲料中添加50mg/kg镉即可显著降低罗非鱼肝胰脏总抗氧化能力,CAT活性最高;添加200mg/kg镉可显著提高罗非鱼肝胰脏MDA含量,SOD活性最高。
This study was conducted to investigate the effects of different levels of dietary Cd on the antioxidant capacity of hepatopancreas of tilapia.300 fish were divided into 5 groups with 4 replicates in each group,and were exposed to dietary Cd at concentrations of 0,50,100,200,400 mg/kg for 8 weeks respectively.The results showed that with the increase of the level of dietary Cd,the content of MDA in hepatapancreas was increased,and the content of MDA in 200 and 400 mg/kg Cd group was significantly higher than that of the control group (P <0.05);the level of T-AOC was decreased with the increase of the level of dietary Cd,and the level of T-AOC in 50 mg/kg Cd group was significantly lower than that of the control group (P <0.05).With the increase of the level of dietary Cd,the activities of CAT and SOD were firstly increased and then decreased,and the highest values were presented in 50 and 200 mg/kg respectively.The results indicated that different levels of dietary Cd had different effects on the antioxidant indices in hepatopancreas of tilapia.Cd in the level of 50 mg/kg in diet of tilapia hepatopancreas could result in the significantly increased T-AOC level and the highest activity of CAT,while the significantly increased content of MDA and the highest activity of SOD in tilapia hepatopancreas was occurred when the level of Cd reached to 200 mg/kg in diet.
This study was conducted to investigate the effects of different levels of dietary Cd on the antioxidant capacity of hepatopancreas of tilapia.300 fish were divided into 5 groups with 4 replicates in each group,and were exposed to dietary Cd at concentrations of 0,50,100,200,400 mg/kg for 8 weeks respectively.The results showed that with the increase of the level of dietary Cd,the content of MDA in hepatapancreas was increased,and the content of MDA in 200 and 400 mg/kg Cd group was significantly higher than that of the control group (P <0.05);the level of T-AOC was decreased with the increase of the level of dietary Cd,and the level of T-AOC in 50 mg/kg Cd group was significantly lower than that of the control group (P <0.05).With the increase of the level of dietary Cd,the activities of CAT and SOD were firstly increased and then decreased,and the highest values were presented in 50 and 200 mg/kg respectively.The results indicated that different levels of dietary Cd had different effects on the antioxidant indices in hepatopancreas of tilapia.Cd in the level of 50 mg/kg in diet of tilapia hepatopancreas could result in the significantly increased T-AOC level and the highest activity of CAT,while the significantly increased content of MDA and the highest activity of SOD in tilapia hepatopancreas was occurred when the level of Cd reached to 200 mg/kg in diet.
引文
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[15]王凡,刘海芳,晋冬梅,等.镉污染对鲤鱼过氧化氢酶活性的影响[J].水产科学,2010,29(9):550-552.
[16]焦传珍,刘小林,瞿飞,等.镧对镉胁迫下鲫鱼肝脏抗氧化酶活力和活性氧含量的影响[J].环境与健康杂志,2009,26(6):528-529.
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[19]王丽丽,夏斌,陈碧鹃,等.镉胁迫对虾夷扇贝抗氧化防御系统的影响[J].海洋环境科学,2012,31(1):39-42.
[20]徐立红,张甬元,陈宜瑜.分子生态毒理学研究进展及其在水环境保护中的意义[J].水生生物学报,1995,19(2):171-185.
[21]Maenair M.Tansley Review No.49:The Genetics of Metal Tolerance in Vaseular Plants[J].New Phytophysio,1993,124(4):541-559.
[22]戴伟.饲料铅对罗非鱼的毒性及硅酸盐纳米级微球减轻其毒害影响的研究[D].杭州:浙江大学,2008.
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[24]贾秀英,陈志伟.镉对鲫鱼组织转氨酶和过氧化氢酶活性的影响[J].环境污染与防治,1997,19(6):4-5.
[25]杨丽华,方展强,郑文彪,等.镉对鲫鱼鳃和肝脏超氧化物歧化酶活性的影响[J].安全与环境学报,2003,3(3):13-15.
[26]丁磊,吴康,张伟明,等.镉对鲫血清抗氧化酶的影响[J].水利渔业,2005,25(6):91-92.
[2]Reddy N,Venugopa K.Effect of cadmium on acetylcholinesterase activity and oxygen consumption in the freshwater field crab,Barytelerusa guerini[J].Journal of Environmental Biology,1993,14:203-210.
[3]陈其晨,张克俭,徐关文.重金属对鱼类毒性的综合研究[J].水产学报,1988,12(l):21-33.
[4]Gomot A.Toxic effects of cadmium on reproduction,development and hatching in the freshwater snail Lymnea stagnalis for water quality monitoring[J].Ecotoxicology and Environmental Safe,1998,41(3):288-297.
[5]Viola A,Pregnola G,Albergoni V.Effect of in vitro cadmium exposure on natural killer(NK)cells of catfish,Ictalurus melas[J].Fish Shellfish Immunology,1996,6(3):167-172.
[6]李涌泉,王兰,刘娜,等.镉对长江华西蟹酶活性及脂质过氧化的影响[J].水生生物学报,2008,32(3):373-379.
[7]秦粉菊,金琎,顾华杰,等.纳米硒对镉胁迫下吉富罗非鱼非特异性免疫和抗氧化功能的影响[J].农业环境科学学报,2011,30(6):1044-1050.
[8]柏世军.水环境镉对罗非鱼的毒性作用和机理探讨[D].杭州:浙江大学,2008.
[9]雍文岳,徐忠法.SC/T1025—2004,中华人民共和国水产行业标准[S].北京:中国标准出版社,2005:1-6.
[10]Rainbow P S.The accumulation of heavy metal in marine organism and its meaning[J].Marine Environmental Science,1992,11(1):44-51.
[11]Stohs S J,Bagchi D,Hassoun E,et al.Oxidative mechanisms in the toxicity of chromium and cadmium ions[J].Environ Pathol Toxicol Oncol,2000,18(2):201-213.
[12]张春玲,胡俊峰,王丕文,等.苯并(a)芘对鲫鱼肝脏总抗氧化能力的影响[J].环境与健康杂志,2004,21(5):325-326.
[13]张朝晖,王婧,黄炼,等.氯化镉染毒对红鲫脂质过氧化作用的影响[J].环境与健康杂志,2010,26(10):906-907.
[14]徐轶磊,熊慧欣,王子波.镉对鲫鱼肝过氧化氢酶活性的影响[J].扬州大学学报,2006,9(3):76-78.
[15]王凡,刘海芳,晋冬梅,等.镉污染对鲤鱼过氧化氢酶活性的影响[J].水产科学,2010,29(9):550-552.
[16]焦传珍,刘小林,瞿飞,等.镧对镉胁迫下鲫鱼肝脏抗氧化酶活力和活性氧含量的影响[J].环境与健康杂志,2009,26(6):528-529.
[17]Messaoudi I,Barhoumi S,Sa d K,et al.Study on the sensitivity to cadmium of marine fish Salaria basilisca(Pisces:Blennidae)[J].Journal of Environmental Sciences,2009,21(11):1620-1624.
[18]胡蓉,段辉国,唐正义.镉和铅对鲫鱼肝胰脏过氧化氢酶活性的影响[J].生命科学研究,2011,15(2):165-169.
[19]王丽丽,夏斌,陈碧鹃,等.镉胁迫对虾夷扇贝抗氧化防御系统的影响[J].海洋环境科学,2012,31(1):39-42.
[20]徐立红,张甬元,陈宜瑜.分子生态毒理学研究进展及其在水环境保护中的意义[J].水生生物学报,1995,19(2):171-185.
[21]Maenair M.Tansley Review No.49:The Genetics of Metal Tolerance in Vaseular Plants[J].New Phytophysio,1993,124(4):541-559.
[22]戴伟.饲料铅对罗非鱼的毒性及硅酸盐纳米级微球减轻其毒害影响的研究[D].杭州:浙江大学,2008.
[23]Bauer R,Demetr I,Hasemann V,et al.Structural properties of the zinc site in Cu,Zn-superoxide dismutase,Perturbed angular correlation of gammaray spectroscopy on the Cu,Cd-superoxide dismutase derivative[J].Biochemical and Biophysical Research Communications,1980,94(4):1296-1302.
[24]贾秀英,陈志伟.镉对鲫鱼组织转氨酶和过氧化氢酶活性的影响[J].环境污染与防治,1997,19(6):4-5.
[25]杨丽华,方展强,郑文彪,等.镉对鲫鱼鳃和肝脏超氧化物歧化酶活性的影响[J].安全与环境学报,2003,3(3):13-15.
[26]丁磊,吴康,张伟明,等.镉对鲫血清抗氧化酶的影响[J].水利渔业,2005,25(6):91-92.