警惕种子传带的南方番茄病毒Southern tomato virus对我国番茄产业的危害
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摘要
南方番茄病毒Southern tomato virus(STV)是近年来新发现的一种侵染番茄的病毒,通常与多种病毒复合侵染,可能与番茄的褪绿、黄化、衰退、果实变小等症状相关。该病毒最早在1984年发现,并于2005年命名。我国首先于2015年在山东寿光发现。最近,我们在山东和北京市多地的番茄样品中均检测到了该病毒,并通过检测发现国内一些主要品种番茄种子的STV携带率达40%。STV基因组为一条双链RNA,隶属Amalgaviridae科Amalgavirus属,是严格种传病毒,不能通过汁液摩擦接种和嫁接传播。鉴于STV的潜在危害以及与其他病毒高度复合侵染造成的严重损失,本文介绍了STV检测方法,并提出应开展对主栽品种的种子检测和针对性防控。
Southern tomato virus(STV) is a recently identified virus infecting tomato, which normally occurs together with a variety of viruses, and may cause chlorosis, yellowing, decline and small fruit. STV was discovered in 1984 and was named in 2005. In China, STV was firstly reported in Shouguang, Shandong province in 2015. We detected STV in tomato samples of Shandong and Beijing, and our work showed that the incidence of STV in seeds of main cultivars reached 40%. Being a member of Amalgavirus in Amalgaviridae, the STV genome is a double-stranded RNA. As a rigorous seed-borne virus, STV cannot be transmitted by mechanical inoculation or grafting. In view of its potential damages and the severe losses induced by high degree of complex infection with other viruses, we introduced the detection method for STV, and recommended that seeds detection and targeted control measures should be carried out.
Southern tomato virus(STV) is a recently identified virus infecting tomato, which normally occurs together with a variety of viruses, and may cause chlorosis, yellowing, decline and small fruit. STV was discovered in 1984 and was named in 2005. In China, STV was firstly reported in Shouguang, Shandong province in 2015. We detected STV in tomato samples of Shandong and Beijing, and our work showed that the incidence of STV in seeds of main cultivars reached 40%. Being a member of Amalgavirus in Amalgaviridae, the STV genome is a double-stranded RNA. As a rigorous seed-borne virus, STV cannot be transmitted by mechanical inoculation or grafting. In view of its potential damages and the severe losses induced by high degree of complex infection with other viruses, we introduced the detection method for STV, and recommended that seeds detection and targeted control measures should be carried out.
引文
[1] LAEMMLEN F F,VAN MAREN A F,ENDO R M,et al.A tomato decline of unknown etiology in Imperial Valley,CA[J].Phytopathology,1985,75:1287.
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[4] SABANADZOVIC S,GHANEM-SABANADZOVIC N A,VALVERDE R A.A novel monopartite dsRNA virus from rhododendron[J].Archives of Virology,2010,155:1859-1863.
[5] SABANADZOVIC S,VALVERDE R A,BROWN J K,et al.Southern tomato virus:The link between the families Totiviridae and Partitiviridae [J].Virus Research,2009,140:130-137.
[6] CANDRESSE T,MARAIS A,FAURE C.First report of Southern tomato virus on tomatoes in southwest France [J].Plant Disease,2013,97:1124.
[7] VERBEEK M,DULLEMANS A M,ESPINO A,et al.First report of Southern tomato virus in tomato in the Canary Islands,Spain [J].Journal of Plant Pathology,2015,97:392.
[8] PADMANABHAN C,YI Zheng,LI Rugang,et al.Complete genome sequence of Southern tomato virus identified in China using next-generation sequencing [J/OL].Genome Announcements,2015,3(5):e01226-15.
[9] PADMANABHAN C,YI Zheng,LI Rugang,et al.Complete genome sequence of Southern tomato virus naturally infecting tomatoes in Bangladesh [J/OL].Genome Announcements,2015,3(6):e01522-15.
[10] 张强,崔百明,郑银英,等.新疆加工番茄上南方番茄病毒外壳蛋白基因的克隆与原核表达[J].西北农林科技大学学报(自然科学版),2015,43(4):118-122.
[11] 苏海娣,梁学超,崔百明,等.新疆加工番茄上一种类似南方番茄病毒的分子检测[J].石河子大学学报(自然科学版),2013,31(3):271-275.
[12] HALGREN A,TZANETAKIS I E,MARTIN R R.Identification,characterization,and detection of a virus associated with decline symptoms in black raspberry[J].Phytopathology,2007,97(1):44-50.
[13] MARTIN R R,ZHOU J,TZANETAKIS I E.Blueberry latent virus:an amalgam of the Partitiviridae and Totiviridae[J].Virus Research,2011,155(1):175-180.
[14] MARTIN R R,TZANETAKIS I E,SWEENEY M,et a1.A virus associated with blueberry fruit drop disease[J].Acta Horticulture,2006,715:497-501.
[15] ISOGAI M,NAKAMURA T,ISHII K,et al.Histochemical detection of Blueberry latent virus in highbush blueberry plant [J].Journal of General Plant Pathology,2011,77(5):304-306.
[2] TURINA M,RICKER M D,LENZI R,et al.A severe disease of tomato in the Culiacan area (Sinaloa,Mexico) is caused by a new picorna-like viral species [J].Plant Disease,2007,91(8):932-941.
[3] VERBEEK M,DULLEMANS A M,VAN DEN HEUVEL J F J M,et al.Identification and characterization of Tomato torrado virus,a new plant picorna-like virus from tomato [J].Archives of Virology,2007,152(5):881-890.
[4] SABANADZOVIC S,GHANEM-SABANADZOVIC N A,VALVERDE R A.A novel monopartite dsRNA virus from rhododendron[J].Archives of Virology,2010,155:1859-1863.
[5] SABANADZOVIC S,VALVERDE R A,BROWN J K,et al.Southern tomato virus:The link between the families Totiviridae and Partitiviridae [J].Virus Research,2009,140:130-137.
[6] CANDRESSE T,MARAIS A,FAURE C.First report of Southern tomato virus on tomatoes in southwest France [J].Plant Disease,2013,97:1124.
[7] VERBEEK M,DULLEMANS A M,ESPINO A,et al.First report of Southern tomato virus in tomato in the Canary Islands,Spain [J].Journal of Plant Pathology,2015,97:392.
[8] PADMANABHAN C,YI Zheng,LI Rugang,et al.Complete genome sequence of Southern tomato virus identified in China using next-generation sequencing [J/OL].Genome Announcements,2015,3(5):e01226-15.
[9] PADMANABHAN C,YI Zheng,LI Rugang,et al.Complete genome sequence of Southern tomato virus naturally infecting tomatoes in Bangladesh [J/OL].Genome Announcements,2015,3(6):e01522-15.
[10] 张强,崔百明,郑银英,等.新疆加工番茄上南方番茄病毒外壳蛋白基因的克隆与原核表达[J].西北农林科技大学学报(自然科学版),2015,43(4):118-122.
[11] 苏海娣,梁学超,崔百明,等.新疆加工番茄上一种类似南方番茄病毒的分子检测[J].石河子大学学报(自然科学版),2013,31(3):271-275.
[12] HALGREN A,TZANETAKIS I E,MARTIN R R.Identification,characterization,and detection of a virus associated with decline symptoms in black raspberry[J].Phytopathology,2007,97(1):44-50.
[13] MARTIN R R,ZHOU J,TZANETAKIS I E.Blueberry latent virus:an amalgam of the Partitiviridae and Totiviridae[J].Virus Research,2011,155(1):175-180.
[14] MARTIN R R,TZANETAKIS I E,SWEENEY M,et a1.A virus associated with blueberry fruit drop disease[J].Acta Horticulture,2006,715:497-501.
[15] ISOGAI M,NAKAMURA T,ISHII K,et al.Histochemical detection of Blueberry latent virus in highbush blueberry plant [J].Journal of General Plant Pathology,2011,77(5):304-306.