GDF9基因12-bp InDel与陕北白绒山羊生长性状关联研究
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摘要
生长分化因子9 (growth differentiation factor 9,GDF9)也称FecG基因,是影响家畜繁殖力的常染色体主效候选基因,此基因在性腺轴相关组织及内脏组织器官中均有表达,且shRNA下调GDF9基因的表达可能对动物生长轴的调控造成影响,因此,推测GDF9基因对动物正常的生长发育也具有重要作用。为探讨GDF9基因作为山羊生长性状候选基因的可能性,寻找与山羊生长性状相关的分子标记,本研究以陕北白绒山羊(n=638)为研究对象,并结合已有报道利用DNA池检测GDF9基因的InDel位点,评估InDel位点与生长性状的相关性。本研究发现,在638只陕北白绒山羊GDF9基因3′调控区存在12-bp的插入缺失(InDel),群体共出现3种基因型(II,ID,DD),其中I和D等位基因频率分别为0.461和0.539,优势基因型为杂合型(ID)频率为0.874。关联性分析结果表明,该突变位点与陕北白绒山羊体高显著相关(P<0.01),同时显著影响十字部高性状(P<0.05)。其他性状虽然没有显著的相关性,但是其体重、体长等重要生长性状也表现相同的趋势。因此,GDF9基因可作为陕北白绒山羊生长性状选育的候选基因,为陕北白绒山羊选育工作提供理论依据。
Growth differentiation factor 9(GDF9),also known as FecG gene,is an autosomal candidate major effect gene that affects fertility,it can express at gonad axis related organization and other organs in sheep.ShRNA down-regulated GDF9 gene expression may affect animal growth axis control.Therefore,GDF9 gene plays an important role in animal of normal growth and development.To explore the possibility of growth differentiation factor 9(GDF9) gene as candidate genes for goat growth traits and to search for molecular markers related to goat growth traits.In this study,Shaanbei white Cashmere(SBWC) goats(n=638) were selected as the research objects.Based on previous reports and use DNA pool to detect the polymorphism of GDF9 gene,and to evaluate the correlation between the InDel site and growth characters.In this study,it was found that there were 12-bp insertion deletion(InDel) in the 3′ regulatory region of GDF9 gene of Shaanbei white Cashmere goats.There were 3 genotypes of type II,ID and DD in the population.The frequency of I and D alleles was 0.461 and 0.539,respectively,and the frequency of heterozygous(ID) was 0.874.Association analysis showed that the 12-bp indel locus had extremely significant effects on the Shaanbei white Cashmere goat body height in this experimental population(P<0.01),and the 12-bp indel locus had significant effects on the Shaanbei white Cashmere goat height at hip cross(P<0.05).Although there was no significant correlation between the other traits,their weight,body length and other important growth traits showed the same trend.In conclusion,GDF9 gene had potential effects on growth traits of Shaanbei white Cashmere goats.Therefore,variations in the GDF9 gene may be useful as major gene for growth traits in Shaanbei white Cashmere goats,which would provide a theoretical basis for breeding of goats.
Growth differentiation factor 9(GDF9),also known as FecG gene,is an autosomal candidate major effect gene that affects fertility,it can express at gonad axis related organization and other organs in sheep.ShRNA down-regulated GDF9 gene expression may affect animal growth axis control.Therefore,GDF9 gene plays an important role in animal of normal growth and development.To explore the possibility of growth differentiation factor 9(GDF9) gene as candidate genes for goat growth traits and to search for molecular markers related to goat growth traits.In this study,Shaanbei white Cashmere(SBWC) goats(n=638) were selected as the research objects.Based on previous reports and use DNA pool to detect the polymorphism of GDF9 gene,and to evaluate the correlation between the InDel site and growth characters.In this study,it was found that there were 12-bp insertion deletion(InDel) in the 3′ regulatory region of GDF9 gene of Shaanbei white Cashmere goats.There were 3 genotypes of type II,ID and DD in the population.The frequency of I and D alleles was 0.461 and 0.539,respectively,and the frequency of heterozygous(ID) was 0.874.Association analysis showed that the 12-bp indel locus had extremely significant effects on the Shaanbei white Cashmere goat body height in this experimental population(P<0.01),and the 12-bp indel locus had significant effects on the Shaanbei white Cashmere goat height at hip cross(P<0.05).Although there was no significant correlation between the other traits,their weight,body length and other important growth traits showed the same trend.In conclusion,GDF9 gene had potential effects on growth traits of Shaanbei white Cashmere goats.Therefore,variations in the GDF9 gene may be useful as major gene for growth traits in Shaanbei white Cashmere goats,which would provide a theoretical basis for breeding of goats.
引文
[1] 邹辉,崔悦悦,夏琴,等.山羊产羔性状相关基因研究进展[J].黑龙江畜牧兽医,2018(11):63-67.
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[8] 胡瑞雪,乐祥鹏,李万宏,等.绵羊产羔数相关基因的研究进展[J].基因组学与应用生物学,2017,36(10):4141-4148.
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[10] 汤继顺,章薇,朱德建,等.GDF9基因多态性对山羊血清FSH和LH水平的影响[J].畜牧与兽医,2017,49(12):13-18.
[11] 杨洁,赫佳,王丹碧,等.InDel标记的研究和应用[J].进展生物多样性,2016,24(2):237-243.
[12] Yang Q,Zhang S,Liu L,et al.Application of mathematical expectation (ME) strategy for detecting low frequency mutations:An example for evaluating 14-bp insertion/deletion (indel) within the bovine PRNP gene[J].Prion,2016,10(5):409-419.
[13] Yang Q,Yan H,Li J,et al.A novel 14-bp duplicated deletion within goat GHR gene is significantly associated with growth traits and litter size[J].Animal Genetics,2017,48(4):499-500.
[14] 马晓萌,轩俊丽,王慧华,等.TXNRD1基因多态性与乌珠穆沁绵羊生长性状的关联分析[J].畜牧兽医学报,2016,47(5):909-921.
[15] 屈雷,雷安民,闫海龙,等.陕北白绒山羊种公羊的体细胞克隆[J].西北农林科技大学学报:自然科学版,2012,40(9):29-34.
[16] 韩丽云.新疆褐牛生长性状及相关基因的关联分析[D].宁夏银川:宁夏大学,2017.
[2] 韩聪,方乾,李浩,等.不同月龄阶段陕北白绒山羊生精细胞发育规律研究[J].家畜生态学报,2018,39(4):43-48.
[3] Cui Y,Yan H,Wang K,et al.Insertion/deletion within the kdm6a gene is significantly associated with litter size in goat[J].Frontiers in Genetics,2018,9,91.
[4] Zamani N,Brown C W.Emerging roles for the transforming growth factor-beta superfamily in regulating adiposity and energy expenditure[J].Endocr Rev,2011,32(3):387-403.
[5] 杨艳,罗飞宏.转化生长因子β超家族介导的脂肪细胞分化新进展[J].医学综述,2017,23(15):2923-2928.
[6] 陶萨茹拉,王峰,刘永斌,等.生长分化因子GDF9基因的研究进展[J].畜牧与饲料科学,2009,30(1):54-55.
[7] 习欠云,焦莉,肖敏,等.GDF9下调转基因小鼠模型对繁殖与生长的影响[J].畜牧兽医学报,2015,46(7):1157-1162.
[8] 胡瑞雪,乐祥鹏,李万宏,等.绵羊产羔数相关基因的研究进展[J].基因组学与应用生物学,2017,36(10):4141-4148.
[9] Wang X,Yang Q,Wang K,et al.A novel 12-bp indel polymorphism within the GDF9 gene is significantly associated with litter size and growth traits in goats[J].Animal Genetics,2017,48(6):735-736.
[10] 汤继顺,章薇,朱德建,等.GDF9基因多态性对山羊血清FSH和LH水平的影响[J].畜牧与兽医,2017,49(12):13-18.
[11] 杨洁,赫佳,王丹碧,等.InDel标记的研究和应用[J].进展生物多样性,2016,24(2):237-243.
[12] Yang Q,Zhang S,Liu L,et al.Application of mathematical expectation (ME) strategy for detecting low frequency mutations:An example for evaluating 14-bp insertion/deletion (indel) within the bovine PRNP gene[J].Prion,2016,10(5):409-419.
[13] Yang Q,Yan H,Li J,et al.A novel 14-bp duplicated deletion within goat GHR gene is significantly associated with growth traits and litter size[J].Animal Genetics,2017,48(4):499-500.
[14] 马晓萌,轩俊丽,王慧华,等.TXNRD1基因多态性与乌珠穆沁绵羊生长性状的关联分析[J].畜牧兽医学报,2016,47(5):909-921.
[15] 屈雷,雷安民,闫海龙,等.陕北白绒山羊种公羊的体细胞克隆[J].西北农林科技大学学报:自然科学版,2012,40(9):29-34.
[16] 韩丽云.新疆褐牛生长性状及相关基因的关联分析[D].宁夏银川:宁夏大学,2017.
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