β-catenin基因26 bp插入/缺失对陕北白绒山羊生长性状的遗传效应分析
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摘要
旨在研究β-连环蛋白(CTNNB1)基因上的多态性位点,并与陕北白绒山羊生长性状进行关联分析,寻找与生长性状相关的遗传标记,为山羊高生产力的标记辅助选择提供科学依据。以陕北白绒山羊(n=848)为研究对象,PCR大样本扩增检测β-catenin基因的插入/缺失(InDel)突变,并评估该位点与生长性状的相关性。结果发现,在848只陕北白绒山羊中β-catenin基因的第10内含子上存在一个26 bp InDel的位点;该群体中共出现3种基因型,分别为II、ID和DD。关联性分析结果表明,该突变位点与陕北白绒山羊胸深显著相关(P<0.05),其中杂合型ID为优势基因型。因此,β-catenin基因可作为陕北白绒山羊生长性状选育的候选基因,为陕北白绒山羊选育工作提供理论依据。
The aim of this study was to investigate the association between the polymorphisms ofβ-catenin and the growth traits of Shaanbei white Cashmere(SBWC)goats.To find the genetic markers associated with growth traits and provide a scientific basis for marker-assisted selection of goats with high productivity.In this study,SBWC goats(n=848)were used as the research object.PCR amplification in SBWC goats was used to detect the InDel mutation ofβ-catenin gene,and the association between the 26 bp InDel and growth traits was evaluated.This study found that there is a 26 bp InDel in the tenth intron of theβ-catenin gene,and there are three genotypes,namelyⅡ,ID and DD.Association analysis showed that the 26 bp InDel was significantly correlated with the chest depth of SBWC goats(P<0.05),and the ID was the dominant genotype.Therefore,β-catenin gene could be used as a candidate gene for breeding traits of SBWC goats,which provided a theoretical basis for the breeding of SBWC goats.
The aim of this study was to investigate the association between the polymorphisms ofβ-catenin and the growth traits of Shaanbei white Cashmere(SBWC)goats.To find the genetic markers associated with growth traits and provide a scientific basis for marker-assisted selection of goats with high productivity.In this study,SBWC goats(n=848)were used as the research object.PCR amplification in SBWC goats was used to detect the InDel mutation ofβ-catenin gene,and the association between the 26 bp InDel and growth traits was evaluated.This study found that there is a 26 bp InDel in the tenth intron of theβ-catenin gene,and there are three genotypes,namelyⅡ,ID and DD.Association analysis showed that the 26 bp InDel was significantly correlated with the chest depth of SBWC goats(P<0.05),and the ID was the dominant genotype.Therefore,β-catenin gene could be used as a candidate gene for breeding traits of SBWC goats,which provided a theoretical basis for the breeding of SBWC goats.
引文
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[21] Zamani N,Brown C W.Emerging roles for the transforming growth factor-beta superfamily in regulating adiposity and energy expenditure[J].Endocr Rev,2011,32(3):387-403.
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[23] 韩聪,方乾,李浩,等.不同月龄阶段陕北白绒山羊生精细胞发育规律研究[J].家畜生态学报,2018,39(4):43-48.
[24] Zhang Q,Jin Y,Jiang F,et al.Relationship between an indel mutation within the SIRT4 gene and growth traits in Chinese cattle[J].Anim Biotechnol,2019,28:1-6.
[25] Van Laere A S,Nguyen M,Braunschweig M,et al.A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig[J].Nature,2003,425:832-836.
[2] 赵有璋.羊生产学[M].北京:中国农业出版社,2011.
[3] 赵宗胜,李大全,李岩,等.对肉羊体型外貌主成分——Fuzzy多级综合评判的探讨[J].中国草食动物,2003(1):9-11.
[4] 翟中和,王喜忠,丁明孝.细胞生物学[M].北京:高等教育出版社,2011:6.
[5] Wang Y,Hong D,Qian Y,et al.Lupeol inhibits growth and migration in two human colorectal cancer cell lines by suppression of Wnt-β-catenin pathway[J].Onco Targets Ther,2018,11:7987-7999.
[6] Timbergen M J M,Janssen M L,Verhoef C,et al.Wnt targets genes are not differentially expressed in desmoid tumors bearing different activating β-catenin mutations[J].Eur J Surg Oncol,2018,S0748-7983(18):31438-31440.
[7] Nayak G,Odaka Y,Prasad V,et al.Developmental vascular regression is regulated by a Wnt/β-catenin,MYC and CDKN1A pathway that controls cell proliferation and cell death[J].Development,2018,145(12).DOI:10.1242/dev.154898.
[8] Zhou L,Zhang T,Sun S,et al.Cryptochrome 1 promotes osteogenic differentiation of human osteoblastic cells via Wnt/β-Catenin signaling[J].Life Sci,2018,212:129-137.
[9] 袁春华,徐劲松.儿童青少年的骨钙、骨密度及骨量与生长发育的关系[J].中国临床康复,2004(24):5092-5094.
[10] González-Cerón F,Rekaya R,Aggrey S E.Genetic analysis of bone quality traits and growth in a random mating broiler population[J].Poult Sci,2015,94(5):883-889.
[11] Li Y,Zhang L,Hu Y,et al.Beta-Catenin directs the transformation of testis Sertoli cells to ovarian granulosa-like cells by inducing Foxl2 expression[J].J Biol Chem,2017,292:17577-17586.
[12] Zhang X L,Yan H L,Wang K,et al.Goat CTNNB1:mRNA expression profile of alternative splicing in testis and association analysis with litter size[J].Gene,2018,679:297-304.
[13] Yang Q,Yan H L,Li J,et al.A novel 14-bp duplicated deletion within goat GHR gene is significantly associated with growth traits and litter size[J].Anim Genet,2017,48(4):499-500.
[14] Wang X Y,Yang Q,Wang K,et al.A novel 12-bp indel polymorphism within the GDF9 gene is significantly associated with litter size and growth traits in goats[J].Anim Genet,2017,48(6):735-736.
[15] Mullaney J M,Mills R E,Pittard W S,et al.Small insertions and deletions (INDELs) in human genomes[J].Hum Mol Genet,2010,19(R2):R131-R136.
[16] 雷雪芹,徐延生,陈宏,等.猪产仔性状的分子标记研究与 QTLs 定位[J].遗传,2001,21(4):263-265.
[17] Jander G,Norris S R,Rounsley S D,et al.Arabidopsis map-based cloning in the post-genome era[J].Plant Physiol,2002,129:440-450.
[18] Kevin P J.Deletion bias in avian introns over evolutionary timescales[J].Mol Biol Evol,2004,21:599-602.
[19] Aljanabi S M,Martinez I.Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques[J].Nucleic Acids Res,1997,25:4692-4693.
[20] Cui Y,Yan H,Wang K,et al.Insertion/deletion within the kdm6a gene is significantly associated with litter size in goat[J].Front Genet,2018,9:91.
[21] Zamani N,Brown C W.Emerging roles for the transforming growth factor-beta superfamily in regulating adiposity and energy expenditure[J].Endocr Rev,2011,32(3):387-403.
[22] 雷耀庚.日粮蛋白水平对陕北白绒山羊种公羊生产性能和繁殖性能的影响[D].陕西杨凌:西北农林科技大学,2015.
[23] 韩聪,方乾,李浩,等.不同月龄阶段陕北白绒山羊生精细胞发育规律研究[J].家畜生态学报,2018,39(4):43-48.
[24] Zhang Q,Jin Y,Jiang F,et al.Relationship between an indel mutation within the SIRT4 gene and growth traits in Chinese cattle[J].Anim Biotechnol,2019,28:1-6.
[25] Van Laere A S,Nguyen M,Braunschweig M,et al.A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig[J].Nature,2003,425:832-836.
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