补肾调轴方调控CD56/CD16改善反复种植失败者子宫免疫微环境的临床研究
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摘要
目的评价补肾调轴方改善体外受精-胚胎移植(IVF-ET)反复种植失败者子宫免疫微环境的临床疗效。方法将47例反复种植失败的患者作为研究对象,予补肾调轴方颗粒进行治疗,检测子宫内膜uNK细胞标记物CD56+、CD16+的表达。结果治疗后CD56+、CD16+的表达较治疗前下降,差异有统计学意义(P<0.05)。结论中药补肾调轴方能下调uNK细胞中CD56+、CD16+表达,促进胚胎免疫耐受,有助于胚胎的着床成功。
Objective:To evaluate the clinical effect of Bushen Tiaozhou Prescription for improving the uterine immune microenvironment in patients undergoing in vitro fertilization-embryo transfer(IVF-ET)repeated implantation. Methods:47 patients who failed in repeated implantation were treated with Bushen Tiaozhou Granule to detect the expression of endometrial uNK cell markers CD56+ and CD16+. Results:The expression of CD56+ and CD16+ decreased after treatment,and the difference was statistically significant. Conclusion:Bushen Tiaozhu Prescription can down-regulate the expression of CD56+ and CD16+ in uNK cells,promote embryonic immune tolerance,and contribute to the successful implantation of embryos.
Objective:To evaluate the clinical effect of Bushen Tiaozhou Prescription for improving the uterine immune microenvironment in patients undergoing in vitro fertilization-embryo transfer(IVF-ET)repeated implantation. Methods:47 patients who failed in repeated implantation were treated with Bushen Tiaozhou Granule to detect the expression of endometrial uNK cell markers CD56+ and CD16+. Results:The expression of CD56+ and CD16+ decreased after treatment,and the difference was statistically significant. Conclusion:Bushen Tiaozhu Prescription can down-regulate the expression of CD56+ and CD16+ in uNK cells,promote embryonic immune tolerance,and contribute to the successful implantation of embryos.
引文
[1]丰有吉.妇产科学[M].北京:人民卫生出版社,2010:412-414.
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[5]季晓微,孙赟.子宫内膜自然杀伤细胞与妊娠关系的研究进展[J].生殖与避孕,2014,34(7):556-559+575.
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[7]张君探,连方,赵地.复方二至天癸颗粒对IVF准备周期子宫内膜Galectin-3的影响研究[J].世界中医药,2012,11(10):1970-1977.
[2]Margalioth EJ,Ben Chetrit A,Gal M,et al.Investigation and treatment of repeated implantation failure following IVF-ET[J].Hum Reprod,2006,21(12):3036-3043.
[3]Ng SC,Gihnan Sachs A,Thaker P,et al.Expression of intracellular Th1 and Th2 cytokines in women with recurrent spontaneous abortion and implantation failures after IVF/ET or normal pregnancy.Am J Reprod Immunol,2002,48(2):77-86.
[4]Kalu E,Bhaskaran S,Thum MY,et al.Sefial estimation of Th1/Th2 cytokines profile in women undergoing in vitro fertilization-embryo transfer.Am J Reprod lmmunol,2008,59(3):206-11.
[5]季晓微,孙赟.子宫内膜自然杀伤细胞与妊娠关系的研究进展[J].生殖与避孕,2014,34(7):556-559+575.
[6]李广文,中国医药学是治疗不孕症的宝库[J].山东中医杂志,1986,(5):29-30.
[7]张君探,连方,赵地.复方二至天癸颗粒对IVF准备周期子宫内膜Galectin-3的影响研究[J].世界中医药,2012,11(10):1970-1977.