Leptospirillum ferriphilum耐砷能力的驯化及驯化机制
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摘要
为了获得耐砷能力较高的中度嗜热嗜酸浸矿菌Leptospirillum ferriphilum,采用连续转接驯化法进行耐砷驯化,并对菌株的驯化机制进行研究。结果表明:驯化后Leptospririllumferriphilum菌的As(Ⅲ)和As(Ⅴ)耐受浓度分别提高到7.0g/L和11.0g/L;As(Ⅴ)驯化菌株对砷黄铁矿的浸出作用明显比As(Ⅲ)驯化菌株强;砷驯化菌细胞官能团发生明显变化,多糖和蛋白质含量明显增多,细胞表面负电荷明显降低,以适应高砷的环境。同步辐射原位C-K边XANES谱图表明,驯化菌糖类和蛋白质的分布位置发生了变化;DFT理论计算表明,驯化菌更容易吸附到矿物表面上。
In order to obtain high arsenic-tolerant ability of Leptospirillum ferriphilum, continuous transfer domestication was adopted, and the acclimation mechanism was studied. The results show that, after domestication, the tolerant abilities to As(Ⅲ) and As(Ⅴ) of L. ferriphilum increase to 7.0 g/L and 11.0 g/L, respectively. The leaching effect of the bacteria acclimated by As(Ⅴ) is significantly higher than that by As(Ⅲ) in the leaching of arsenopyrite. After being acclimated,the contents of polysaccharide and protein of cells significantly increase, while the negative charge on the surface of cells significantly decreases, so as to adapt to the high arsenic environment. The in-situ C-K-edge XANES results show a change in the distribution of polysaccharide and protein. The DFT calculation shows that the adapted bacterial cells are easier to be adsorbed on the mineral surface.
In order to obtain high arsenic-tolerant ability of Leptospirillum ferriphilum, continuous transfer domestication was adopted, and the acclimation mechanism was studied. The results show that, after domestication, the tolerant abilities to As(Ⅲ) and As(Ⅴ) of L. ferriphilum increase to 7.0 g/L and 11.0 g/L, respectively. The leaching effect of the bacteria acclimated by As(Ⅴ) is significantly higher than that by As(Ⅲ) in the leaching of arsenopyrite. After being acclimated,the contents of polysaccharide and protein of cells significantly increase, while the negative charge on the surface of cells significantly decreases, so as to adapt to the high arsenic environment. The in-situ C-K-edge XANES results show a change in the distribution of polysaccharide and protein. The DFT calculation shows that the adapted bacterial cells are easier to be adsorbed on the mineral surface.
引文
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[21]赵花荣,王晓燕,陈冠华,温树敏.利用傅里叶变换红外光谱法鉴定小麦品种[J].光谱学与光谱分析,2004,24(11):1338-1341.ZHAO Hua-rong,WANG Xiao-yan,CHEN Guan-hua,WENShu-ming.Identification of wheat varieties by Fourier transform infrared spectroscopy[J].Spectroscopy and Spectral Analysis,2004,24(11):1338-1341.
[22]郭振楚,韩永生,封惠侠.三种多糖的光谱鉴定、化学改性及活性[J].光谱学与光谱分析,1999,19(1):25-27.GUO Zhen-chu,HAN Yong-sheng,FENG Hui-xia.Spectral identification,chemical modification and activity of three polysaccharides[J].Spectroscopy and Spectral Analysis,1999,19(1):25-27.
[23]周群,孙素琴,杜德国,梁曦云,杨显荣.芦丁热稳定性的红外光谱法实时原位跟踪[J].光谱学与光谱分析,2000,20(2):195-198.ZHOU Qun,SUN Su-qing,DU De-guo,LIANG Xi-yun,YANG Xian-rong.The thermal stability of rutin in situ tracking by infrared spectroscopy[J].Spectroscopy and Spectral Analysis,2000,20(2):195-198.
[24]蔡林,王革娇.抗砷性微生物及其抗砷分子机制研究进展[J].微生物学通报,2009,36(8):1253-1259.CAI Lin,WANG Ge-jiao.Advance on studies of arsenic-resistant microorganisms and molecular mechanisms[J].Microbiology,2009,36(8):1253-1259.
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