姜黄素对挤压综合征大鼠肾损伤及JNK信号通路活化抑制作用的实验研究

详细信息    查看全文 | 推荐本文 |

英文篇名：Experimental Study on Inhibition Effect of Curcumin on Renal Injury and Activation of JNK Signaling Pathway in Rats with Crush Syndrome 作者：张春杰 ; 蔡乾荣 ; 吴颀 ; 陈剑 ; 殷洁峰 ; 张喆 ; 王小栋 英文作者：ZHANG Chunjie;CAI Qianrong;WU Qi;CHEN Jian;YIN Jiefeng;ZHANG Zhe;WANG Xiaodong;Department of General Surgery,Tongde Hospital of Zhejiang Province; 关键词：挤压综合征 ; 肾损伤 ; JNK信号通路活化 ; 姜黄素 ; 大鼠 英文关键词：crush syndrome;;kidney injury;;activation of JNK signal pathway;;curcumin;;rat 中文刊名：TJYY 英文刊名：Chinese Journal of Traditional Medical Science and Technology 机构：浙江省立同德医院普外科; 出版日期：2017-09-20 出版单位：中国中医药科技 年：2017 期：v.24 基金：浙江省中医药科技计划项目(2014ZA013) 语种：中文; 页：TJYY201705013 页数：4 CN：05 ISSN：23-1353/R 分类号：45-48

摘要

目的:观察姜黄素对挤压综合征大鼠肾损伤和JNK信号通路活化的抑制作用。方法:重物挤压建立大鼠挤压综合征模型,实验分为正常组、模型组、姜黄素低剂量组、姜黄素高剂量组,造模前2 h腹腔注射给药,观察各组大鼠肾组织及肌肉组织的病理变化,在造模6、12、24 h分别检测肾功指标(BUN、SCr),Western blot法检测各组大鼠肾组织JNK信号通路活化水平。结果:除正常组外,其余各组大鼠均可见肌肉和肾脏损伤,姜黄素组损伤较模型组为轻。与正常组比较,各造模组血清BUN、SCr水平明显升高(P<0.05),肾组织p-JNK表达明显增强(P<0.05),随挤压伤时间延长呈加重趋势;姜黄素组血清BUN、SCr水平较模型组降低(P<0.05),肾组织p-JNK表达明显减弱(P<0.05),高剂量组效果更显著。结论:姜黄素可以有效地减轻挤压综合征大鼠肾损伤,抑制肾组织JNK信号通路活化。
        Objective: To investigate the effect of Curcumin on renal injury and activation of JNK signaling pathway in rats of crush syndrome. Methods: Models with crush syndrome were built by heavy extrusion,experiment was divided into normal,model and Curcumin low,high dose groups,Curcumin was given to rats by intraperitoneal injection before modeling 2 hours. The pathological changes of crushed muscle and kidney were observed,serum BUN and SCr levels were determined after modeling 6,12,24 hours,c-jun protein expression in kidney tissue was determined by Western blot method. Results: The muscle and kidney injuries were obvious in modeling rats except normal group,those in Curcumin groups were slighter than those in model group. To compare with normal group,the serum BNU and SCr levels were significantly raised in modeling rats,p-JNK expression in renal tissue was enhanced significantly,these changes got worse with time; serum BNU and SCr levels of Curcumin groups were lower than those in model group,p-JNK expression in renal tissue was abated,the effects of high dose group were more significant( P < 0. 05). Conclusion: Curcumin can effectively reduce kidney injury in Crush syndrome rats,inhibit the activation of JNK signal pathway in renal tissue.

引文

[1]唐怡,张凌,杨莹莹,等.连续性静脉-静脉血液滤过治疗山羊挤压综合征模型的建立[J].四川大学学报(医学版),2016,47(1):28-32.
    [2]刘芳,付平,陶冶,等.地震灾害后挤压综合征及急性肾功能衰竭救治-汶川地震特稿[J].中国实用内科杂志,2008,28(7):598-600.
    [3]Sever M S,Vanholder R.Management of crush victims in mass disasters:highlights from recently published recommendations[J].Clin J Am Soc Nephrol,2013,8(2):328-335.
    [4]王鑫.挤压伤-挤压综合征治疗的研究进展[J].中华灾害救援医学,2016,4(8):466-469.
    [5]Strauss M B.The effect of hyperbaric oxygen in crush injuries and skeletal muscle-compartment syndromes[J].Undersea Hyperb Med,2012,39(4):847-855.
    [6]王道周,裘志成,杨艳,等.姜黄素对2型糖尿病肾病大鼠保护作用及机制研究[J].中国免疫学杂志,2017,33(4):588-592.
    [7]田华,母传贤,尤丽菊,等.姜黄素对糖尿病肾病大鼠肾脏的保护作用及其机制[J].吉林大学学报(医学版),2013,39(4):747-750,866.
    [8]潘鑫,邹圣强,苏兆亮,等.双下肢不同时间大鼠挤压伤-挤压综合征模型生化与病理观察[J].中国矫形外科杂志,2015,23(2):156-159.
    [9]刘涛,白祥军.挤压伤和挤压综合征[J].创伤外科杂志,2016,18(7):447-449.
    [10]魏茜,许先武,马芹.挤压综合征的表现和救治[J].世界最新医学信息文摘,2015,15(7):80-81.
    [11]Song XB,Zhou XL,Ni SZ,et al.Early fluid resuscitation with hypertonic solution in a rat crush injury model[J].Shock,2013,39(5):453-459.
    [12]刘承,钟毅,冯健,等.姜黄素抗动脉粥样硬化及其危险因素的作用与机制回顾[J].中药新药与临床药理,2017,28(2):257-262.
    [13]张忠敏,王晓莉,毕鹏翔,等.姜黄素对阿尔兹海默病细胞模型的保护作用及GAP-43蛋白表达的影响[J].国际药学研究杂志,2017,44(3):257-261.
    [14]陈君,焦德敏,唐夏莉,等.姜黄素通过下调necin-4抑制肺癌PC-9细胞的迁移和侵袭[J].中国病理生理杂志,2017,33(2):271-277.
    [15]胡辉,荆绪斌,蔡先彬,等.姜黄素氧化损伤线粒体诱导肝癌SMMC-7721细胞凋亡的研究[J].重庆医学,2012,41(3):269-274.
    [16]陈国钦,黎锦亮,张稳柱,等.姜黄素对过氧化氢诱导内皮细胞凋亡及衰老的影响[J].广东医学,2017,38(8):1155-1158.
    [17]刘晓丽,马礼鸿,王全义,等.XIAP,c-jun在肺癌组织中的表达及其意义[J].第三军医大学学报,2012,34(23):2408-2410.