小儿解感颗粒体内外抗呼吸道合胞病毒实验研究
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摘要
目的:研究小儿解感颗粒体内外对呼吸道合胞病毒(RSV)感染的影响及意义。方法:体外实验用人喉癌细胞(Hep-2)培养法,以治疗指数(TI)为研究指标,以利巴韦林为阳性对照药,研究不同剂量小儿解感颗粒对RSV的抑制作用;体内实验选用BALB/c小鼠,运用肺指数检测法、HE染色法、ELISA检测法研究该药体内抗RSV作用。结果:体外实验,小儿解感颗粒TC50=1.65 mg/mL,TC0=1.25 mg/mL,TI=5.06;体内实验,与模型组相比,小儿解感颗粒高、中剂量组肺指数显著下降(P<0.05);HE染色观察得出小儿解感颗粒高剂量组与正常组形态最为接近;ELISA检测得出,用药组白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)含量随剂量的增大而减少,且均比利巴韦林组效果显著,小儿解感颗粒高剂量组TNF-α含量明显降低(P<0.05)。结论:小儿解感颗粒体外抑制RSV作用明显,体内对RSV引起的疾病有明显治疗作用。
Objective:To study the effect and significance of anti-respiratory syncytial virus(RSV) of Xiaoer Jiegan Granules in vitro and in vivo. Methods:In vitro experiments,using human laryngeal cancer cells(hep-2) culture method,with the treatment index(TI) as the research index,elibavirin as the positive control drug,the inhibitory effect of Xiaoer Jiegan Granules with different dosage on RSV was studied. BALB/c mice were selected for the in vivo experiment,and the anti-RSV effect of this drug in vivo was studied by lung index detection method,HE staining method and ELISA detection method. Results:In vitro,Xiaoer Jiegan Granules TC0=1.25 mg/mL,TC50=1.65 mg/mL,TI=5.06.In vivo,compared with the model group,the lung index of Xiaoer Jiegan Granules high and medium dose groups decreased significantly(P <0.05). HE staining method showed that the morphology of Xiaoer Jiegan Granules high-dose group was the closest to that of the normal group. ELISA detection method showed that the content of IL-6 and TNF-αin the treatment group decreased with the increase of dose,and the effect was significantly higher than that in the bavirin group,while the content of TNF-α in Xiaoer Jiegan Granules high-dose group decreased significantly(P<0.05). Conclusion:Xiaoer Jiegan Granules has obvious inhibitory effect on RSV in vitro,and it has obvious therapeutic effect on RSV-induced diseases in vivo.
Objective:To study the effect and significance of anti-respiratory syncytial virus(RSV) of Xiaoer Jiegan Granules in vitro and in vivo. Methods:In vitro experiments,using human laryngeal cancer cells(hep-2) culture method,with the treatment index(TI) as the research index,elibavirin as the positive control drug,the inhibitory effect of Xiaoer Jiegan Granules with different dosage on RSV was studied. BALB/c mice were selected for the in vivo experiment,and the anti-RSV effect of this drug in vivo was studied by lung index detection method,HE staining method and ELISA detection method. Results:In vitro,Xiaoer Jiegan Granules TC0=1.25 mg/mL,TC50=1.65 mg/mL,TI=5.06.In vivo,compared with the model group,the lung index of Xiaoer Jiegan Granules high and medium dose groups decreased significantly(P <0.05). HE staining method showed that the morphology of Xiaoer Jiegan Granules high-dose group was the closest to that of the normal group. ELISA detection method showed that the content of IL-6 and TNF-αin the treatment group decreased with the increase of dose,and the effect was significantly higher than that in the bavirin group,while the content of TNF-α in Xiaoer Jiegan Granules high-dose group decreased significantly(P<0.05). Conclusion:Xiaoer Jiegan Granules has obvious inhibitory effect on RSV in vitro,and it has obvious therapeutic effect on RSV-induced diseases in vivo.
引文
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[6]刘相文,田景振,袁琦,等.中药金樱子不同溶剂萃取物抗病毒活性分析[J].药物分析杂志,2017,37(12):2196-2201.
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[12]梁羽茜,刘晓丽,张琳婧,等.金莲花汤不同提取物对细胞增殖的影响及体外抗病毒活性研究[J].医学研究杂志,2018,47(4):100-106.
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[14]彭志,卢芳国,屈金艳,等.银黄清肺胶囊含药血清体外抗呼吸道合胞病毒实验研究[J].湖南中医药大学学报,2016,36(2):40-43.
[15]朱秀珍,申文伟,龚翠莹,等.异荭草苷体内外抗呼吸道合胞病毒活性研究[J].中山大学学报(医学科学版),2015,36(3):352-359.
[16]邱欢,李振兴,朱童娜,等.疏风解毒胶囊体内抗病毒作用的实验研究[J].中药新药与临床药理,2014,25(1):14-17.
[17]赵荣华,郭姗姗,高英杰,等.小儿清瘟解热颗粒体内外抗病毒作用及其机制分析[J].中国实验方剂学杂志,2015,21(22):128-132.
[18]丁月文,曾丽娟,李润峰,等.连花清瘟颗粒抗呼吸道合胞病毒感染BALB/c小鼠的药效作用研究[J].广州中医药大学学报,2016,33(4):540-544.
[19]施恒飞.黄芩苷抗呼吸道合胞病毒感染作用研究[D].南京:南京大学,2016.
[20]朱秀珍,申文伟,龚翠莹,等.异荭草苷体内外抗呼吸道合胞病毒活性研究[J].中山大学学报(医学科学版),2015,36(3):352-359.
[21] EROGLU E,SINGH A,BAWAGE S,et al. Immunogenicity of RSV F DNA vaccine in BALB/c mice[J]. Adv Virol,2016,2016:7971847.
[22] ZHU T,ZHANG C,YU L,et al. The preventive effect of vaccine prophylaxis on severe respiratory syncytial virus infection:a meta-analysis[J]. Virol Sin,2015,30(5):371-378.
[23] GLICK A F,KJELLEREN S,HOFSTETTER A M,et al.RSV hospitalizations in comparison with regional RSV activity and inpatient palivizumab administration,2010-2013[J]. Hosp Pediatr,2017,7(5):271-278.
[24]王菲,张书文.升清降浊丸对免疫性肝损伤模型小鼠细胞因子TNF-α、IL-4、IL-6、IFN-γ的影响[J].世界中西医结合杂志,2007,2(4):210-212.
[25]申健,王瑞琳,徐瑾,等.类风湿性关节炎滑膜组织中IFN-γ和IL-4的表达及意义[J].临床与实验病理学杂志,2014,30(5):519-522.
[26]魏盼,季律,袁为远,等.麻黄-甘草药对对小鼠脾细胞的增殖以及Th1/Th2平衡的影响[J].南京中医药大学学报,2018,35(4):371-375,380.
[2] MIGUEZ A,IFTIMI A,MONTES F. Temporal association between the influenza virus and respiratory syncytial virus(RSV):RSV as a predictor of seasonal influenza[J].Epidemiol Infect,2016,144(12):2621-2632.
[3]农棉外.小儿解感颗粒治疗小儿急性上呼吸道感染的疗效观察[J].实用心脑肺血管病杂志,2013,21(7):137-138.
[4]肖飞,赵厚睿,彭文静,等.小儿解感颗粒的临床应用经验探讨[J].世界中医药,2017,12(1):128-129,133.
[5]郭彬芳,肖敏.小儿解感颗粒治疗小儿急性上呼吸道感染的疗效观察[J].食品与药品,2016,18(4):286-287.
[6]刘相文,田景振,袁琦,等.中药金樱子不同溶剂萃取物抗病毒活性分析[J].药物分析杂志,2017,37(12):2196-2201.
[7]李俊英,李际强.苏黄止咳胶囊对呼吸道合胞病毒作用的体外实验研究[J].河北中医,2017,39(4):575-578.
[8]覃黎葵,张奉学,黄笑娟,等.银翘散对呼吸道合胞病毒感染小鼠呼吸系统的作用研究[J].新中医,2018,50(5):24-30.
[9]管仲莹,李国信,孟莉,等.射干有效成分体外抗病毒实验研究[J].中华中医药学刊,2015,33(8):1814-1816.
[10]袁琦,侯林,崔清华,等.红景天甲醇冷浸提取物的体外抗病毒谱及抗病毒机制的研究[J].中华中医药学刊,2017,35(9):2345-2348.
[11]王学文.复方中药制剂对甲型流感病毒感染的抑制作用及其作用机制的研究[D].北京:中国农业大学,2017.
[12]梁羽茜,刘晓丽,张琳婧,等.金莲花汤不同提取物对细胞增殖的影响及体外抗病毒活性研究[J].医学研究杂志,2018,47(4):100-106.
[13]廖卫波,申宝德,沈成英,等.绿原酸与甘草酸联合应用体外抗呼吸道合胞病毒作用研究[J].解放军药学学报,2017,33(1):12-16.
[14]彭志,卢芳国,屈金艳,等.银黄清肺胶囊含药血清体外抗呼吸道合胞病毒实验研究[J].湖南中医药大学学报,2016,36(2):40-43.
[15]朱秀珍,申文伟,龚翠莹,等.异荭草苷体内外抗呼吸道合胞病毒活性研究[J].中山大学学报(医学科学版),2015,36(3):352-359.
[16]邱欢,李振兴,朱童娜,等.疏风解毒胶囊体内抗病毒作用的实验研究[J].中药新药与临床药理,2014,25(1):14-17.
[17]赵荣华,郭姗姗,高英杰,等.小儿清瘟解热颗粒体内外抗病毒作用及其机制分析[J].中国实验方剂学杂志,2015,21(22):128-132.
[18]丁月文,曾丽娟,李润峰,等.连花清瘟颗粒抗呼吸道合胞病毒感染BALB/c小鼠的药效作用研究[J].广州中医药大学学报,2016,33(4):540-544.
[19]施恒飞.黄芩苷抗呼吸道合胞病毒感染作用研究[D].南京:南京大学,2016.
[20]朱秀珍,申文伟,龚翠莹,等.异荭草苷体内外抗呼吸道合胞病毒活性研究[J].中山大学学报(医学科学版),2015,36(3):352-359.
[21] EROGLU E,SINGH A,BAWAGE S,et al. Immunogenicity of RSV F DNA vaccine in BALB/c mice[J]. Adv Virol,2016,2016:7971847.
[22] ZHU T,ZHANG C,YU L,et al. The preventive effect of vaccine prophylaxis on severe respiratory syncytial virus infection:a meta-analysis[J]. Virol Sin,2015,30(5):371-378.
[23] GLICK A F,KJELLEREN S,HOFSTETTER A M,et al.RSV hospitalizations in comparison with regional RSV activity and inpatient palivizumab administration,2010-2013[J]. Hosp Pediatr,2017,7(5):271-278.
[24]王菲,张书文.升清降浊丸对免疫性肝损伤模型小鼠细胞因子TNF-α、IL-4、IL-6、IFN-γ的影响[J].世界中西医结合杂志,2007,2(4):210-212.
[25]申健,王瑞琳,徐瑾,等.类风湿性关节炎滑膜组织中IFN-γ和IL-4的表达及意义[J].临床与实验病理学杂志,2014,30(5):519-522.
[26]魏盼,季律,袁为远,等.麻黄-甘草药对对小鼠脾细胞的增殖以及Th1/Th2平衡的影响[J].南京中医药大学学报,2018,35(4):371-375,380.
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