黄淮海地区大豆主栽品种对8个大豆疫霉菌株的抗性评价
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摘要
随着麦茬免耕栽培技术的推广应用,黄淮海地区麦后夏播大豆生产中疫霉根腐病呈加重趋势。了解该地区大豆主栽品种对疫霉根腐病的抗性和筛选抗病亲本,对培育新的高产广适抗病品种具有重要意义。本研究利用8个具有不同毒力的大豆疫霉菌株,采用下胚轴创伤接种法,对20世纪50年代以来黄淮海地区审定、推广的140个大豆主栽品种进行接种鉴定。表明除6个品种对8个菌株均无抗性外,其余134个品种分别抗1~8个大豆疫霉菌株,占鉴定品种总数的95.7%,其中抗6~8个以上菌株的品种有83个,占鉴定品种总数的59.3%。以14个鉴别寄主的抗病反应型为参照,发现134个品种对8个大豆疫霉菌株共产生65种反应型,其中19个品种产生的5种反应型与已知单基因或2个单基因组合反应型相同;115个品种产生的60种反应型与含有已知单基因或2个单基因组合的反应型不同,推测可能含有新的抗病基因或基因组合。根据研究结果合理选择亲本,可培育出聚合多个抗性基因且综合性状优良的大豆新品种。
With the application of wheat stubble no-tillage cultivation technology,the occurrence rate of phytophthora root rot of soybean is getting higher in the Huang-Huai-Hai Rivers Valley.Therefore,it is crucial to investigate the resistance of the widely-grown soybean cultivars to phytophthora root rot and to screen disease-resistant resources for soybean improvement.In the current study,a total of 140 soybean cultivars,widely-grown in Huang-Huai-Hai Rivers Valley since the 1950 s,were investigated for their response to eight strains of Phytophthora sojae through the hypocotyl inoculation method.Except that six cultivars were susceptible to all strains,134 cultivars(95.7%) were resistant to 1–8 strains,including 83 cultivars(59.3%) resistant to 6–8 strains.A total of 65 reaction types in response to the eight strains were identified from the 134 resistant cultivars by means of 14 reference genotypes.Five reaction types produced by 19 cultivars fit the reaction-type profile of a known resistance gene or the combination of two genes.The remaining 60 reaction types by 115 cultivars were different from all reaction types of a single resistance gene or the combinations of two genes,indicating that theses cultivars might carry novel resistance gene(s)/gene combination(s) against P.sojae.These results provide a guide in parent selection for pyramiding resistance genes in soybean cultivars with good agronomic backgrounds.
With the application of wheat stubble no-tillage cultivation technology,the occurrence rate of phytophthora root rot of soybean is getting higher in the Huang-Huai-Hai Rivers Valley.Therefore,it is crucial to investigate the resistance of the widely-grown soybean cultivars to phytophthora root rot and to screen disease-resistant resources for soybean improvement.In the current study,a total of 140 soybean cultivars,widely-grown in Huang-Huai-Hai Rivers Valley since the 1950 s,were investigated for their response to eight strains of Phytophthora sojae through the hypocotyl inoculation method.Except that six cultivars were susceptible to all strains,134 cultivars(95.7%) were resistant to 1–8 strains,including 83 cultivars(59.3%) resistant to 6–8 strains.A total of 65 reaction types in response to the eight strains were identified from the 134 resistant cultivars by means of 14 reference genotypes.Five reaction types produced by 19 cultivars fit the reaction-type profile of a known resistance gene or the combination of two genes.The remaining 60 reaction types by 115 cultivars were different from all reaction types of a single resistance gene or the combinations of two genes,indicating that theses cultivars might carry novel resistance gene(s)/gene combination(s) against P.sojae.These results provide a guide in parent selection for pyramiding resistance genes in soybean cultivars with good agronomic backgrounds.
引文
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[2]罗永藩.我国少耕与免耕技术推广应用情况与发展前景.耕作与栽培,1991,(2):1–7Luo Y F.The popularization and application of less tillage and no tillage technology in China.GENGZUO YU ZAIPEI,1991,(2):1–7(in Chinese)
[3]田慧,谭周进,屠乃美,李建国,肖启明.少免耕土壤生态学效应研究进展.耕作与栽培,2006,(5):10–12Tian H,Tan Z J,Tu N M,Li J G,Xiao Q M.Research progress on soil ecological effects of no tillage.GENGZUO YU ZAIPEI,2006,(5):10–12(in Chinese)
[4]朱振东,王晓鸣,戴法超.大豆疫霉根腐病在我国的发生及防治对策.植物保护,1999,25(3):47–49Zhu Z D,Wang X M,Dai F C.Occurrence and control of phytophthora root rot of soybean in China.Plant Prot,1999,25(3):47–49(in Chinese)
[5]Sun J T,Li L H,Zhao J M,Huang J,Yan Q,Xing H,Guo N.Genetic analysis and fine mapping of Rps JS,a novel resistance gene to Phytophthora sojae in soybean[Glycine max(L.)Merr.].Theor Appl Genet,2014,127:913–919
[6]Sugimoto T,Kato M,Yoshida S,Matsumoto I,Kobayashi T,Kaga A,Hajika M,Yamamoto R,Watanabe K,Aino M,Matoh T,Walker D R,Biggs A R,Ishimoto M.Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans.Breed Sci,2012,61:511–522
[7]Weng C,Yu K,Anderson T R,Poysa V.Mapping genes conferring resistance to phytophthora root rot of soybean,Rps1a and Rps7.J Hered,2001,92:442–446
[8]Burnham K D,Dorrance A E,Francis D M,Fioritto R J,Martin S K St.Rps8,a new locus in soybean for resistance to Phytophthora sojae.Crop Sci,2003,43:101–105
[9]Gordon S G,Martin S K St,Dorrance A E.Rps8 maps to a resistance gene rich region on soybean molecular linkage group F.Crop Sci,2006,46:168–173
[10]Wu X L,Zhang B Q,Sun S,Zhao J M,Yang F,Guo N,Gai J Y,Xing H.Identification,genetic analysis and mapping of resistance to Phytophthora sojae of Pm28 in soybean.Sci Agric Sin,2011,10:1506–1511
[11]Zhang J Q,Xia C J,Duan C X,Sun S L,Wang X M,Wu X F,Zhu Z D.Identification and candidate gene analysis of a novel Phytophthora resistance gene Rps10 in a Chinese soybean cultivar.PLo S One,2013,8:e69799
[12]朱振东,霍云龙,王晓鸣,黄俊斌,武小菲.一个抗大豆疫霉根腐病新基因的分子鉴定.作物学报,2007,33:154–157Zhu Z D,Huo Y L,Wang X M,Huang J B,Wu X F.Molecular identification of a novel Phytophthora resistance gene in soybean.Acta Agron Sin,2007,33:154–157(in Chinese with English abstract)
[13]Sun S,Wu X L,Zhao J M,Wang Y C,Tang Q H,Yu D Y,Gai J Y,Xing H.Characterization and mapping of Rps Yu25,a novel resistance gene to Phytophthora sojae.Plant Breed,2011,130:139–143
[14]范爱颖,王晓鸣,方小平,武小菲,朱振东.大豆品种豫豆25抗疫霉根腐病基因的鉴定.作物学报,2009,35:1844–1850Fan A Y,Wang X M,Fang X P,Wu X F,Zhu Z D.Molecular identification of Phytophthora resistance gene in soybean cultivar Yudou25.Acta Agron Sin,2009,35:1844–1850(in Chinese with English abstract)
[15]姚海燕,王晓鸣,武小菲,肖炎农,朱振东.大豆品种早熟18抗疫霉根腐病基因的SSR分子标记.植物遗传资源学报,2010,11:213–217Yao H Y,Wang X M,Wu X F,Xiao Y N,Zhu Z D.Molecular mapping of Phytophthora resistance gene in soybean cultivar Zaoshu 18.J Plant Genet Resour,2010,11:213–217(in Chinese with English abstract)
[16]于安亮,徐鹏飞,王金生,张淑珍,吴俊江,李文滨,陈维元,李宁辉,范素杰,王欣,姜良宇.大豆品种绥农10抗疫霉根腐病遗传分析及抗病基因的SSR标记.中国油料作物学报,2010,32:462–466Yu A L,Xu P F,Wang J S,Zhang S Z,Wu J J,Li W B,Chen W Y,Li N H,Fan S J,Wang X,Jiang L Y.Genetic analysis and SSR mapping of gene resistance to Phytophthora sojae race 1 in soybean cv Suinong 10.Chin J Oil Crop Sci,2010,32:462–466(in Chinese with English abstract)
[17]武晓玲,周斌,孙石,赵晋铭,陈受宜,盖钧镒,邢邯.大豆对大豆疫霉菌株Pm14抗性的遗传分析及基因定位.中国农业科学,2011,44:456–460Wu X L,Zhou B,Sun S,Zhao J M,Chen S Y,Gai J Y,Xing H.Genetic analysis and mapping of resistance to Phytophthora sojae of Pm14 in soybean.Sci Agric Sin,2011,44:456–460(in Chinese with English abstract)
[18]Zhang J Q,Xia C J,Wang X M,Duan C X,Sun S L,Wu X F,Zhu Z D.Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar.Theor Appl Genet,2013,126:1555–1561
[19]Lin F,Zhao M X,Ping J Q,Johnson A,Zhang B,Abney T S,Hughes T J,Ma J X.Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI567139B.Theor Appl Genet,2013,126:2177–2185
[20]Ping J Q,Fitzgerald J C,Zhang C B,Lin F,Bai Y H,Wang D C,Aggarwal R,Rehman M,Crasta O,Ma J X.Identification and molecular mapping of Rps11,a novel gene conferring resistance to Phytophthora sojae in soybean.Theor Appl Genet,2016,129:445–451
[21]Sahoo D K,Abeysekara N S,Cianzio S R,Robertson A E,Bhattacharyya M K.A novel Phytophthora sojae resistance Rps12 gene mapped to a genomic region that contains several Rps genes,PLo S One,2017,12:e0169950
[22]Li Y P,Sun S L,Zhong C,Wang X M,Wu X F,Zhu Z D.Genetic mapping and development of co-segregating markers of Rps Q,which provides resistance to Phytophthora sojae in soybean.Theor Appl Genet,2017,130:1223-1233
[23]Cheng Y B,Ma Q B,Ren H L,Xia Q J,Song E L,Tan Z Y,Li S X,Zhang G Y,Nian H.Fine mapping of a Phytophthora-resistance gene Rps WY in soybean(Glycine max L.)by highthroughput genome-wide sequencing.Theor Appl Genet,2017,130:1041–1051
[24]Schmitthenner A F.Problems and progress in control of Phytophthora root rot of soybean.Plant Dis,1985,69:362–368
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