天龙通心片对心肌缺血再灌注损伤及血液流变学的影响
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摘要
目的:探索天龙通心片对大鼠心肌缺血再灌注损伤的保护作用,并观察其对大鼠血栓形成、血液黏度及家兔血小板聚集的影响。方法:取56只Wistar大鼠,除假手术组外,建立大鼠心肌缺血再灌注损伤模型,造模成功的模型分为模型组、合心爽组、复方丹参片组、天龙通心片4,2,1 g·kg~(-1)组,每组8只;硝基四氮唑蓝(N-BT)法观察受试药对心肌梗塞程度的改善作用;比色法检测受试药对血清超氧化物歧化酶(SOD),丙二醛(MDA)的影响。采用Chandler体外法检测正常组、天龙通心片4,2,1 g·kg~(-1)组、复方丹参片组、阿司匹林组大鼠体外血栓形成及血液黏度。Born氏比浊法观察正常组、天龙通心片2,1,0. 5 g·kg~(-1)组、复方丹参片组、阿司匹林组家兔血小板聚集变化水平。结果:与假手术组比较,模型组心肌梗死面积明显增大,血清SOD降低,MDA升高,均有显著性差异(P <0. 05);与模型组比较,天龙通心片各剂量组、合心爽组、复方丹参片组均能够减轻心肌梗塞程度,梗塞区占心室及心脏百分比降低,均有显著性差异(P <0. 05,P <0. 01);与正常组比较,天龙通心片4,2 g·kg~(-1)组、复方丹参片组、阿司匹林片组均能明显缩短血栓长度(P <0. 05,P <0. 01),明显减轻血栓湿重和干重(P <0. 05,P <0. 01);且明显降低切变率150,60,5 s~(-1)下的全血黏度(P <0. 05,P <0. 01);与正常组比较,天龙通心片2,1 g·kg~(-1)组、复方丹参片组、阿司匹林片组均能显著降低胶原,二磷酸腺苷(ADP),花生四烯酸(AA)诱导的家兔血小板聚集率(P <0. 05,P <0. 01)。结论:天龙通心片能保护大鼠心肌缺血再灌注损伤,并且具有抑制血小板聚集和血栓形成,降低血液黏度的作用。
Objective: To explore the protective effect of Tianlong Tongxin tablet on myocardial ischemia reperfusion injury in rats,and observe its effect on thrombosis,blood viscosity and platelet aggregation in rabbits.Method: Totally 56 Wistar rats were collected. Except for the sham operation group,all of the remaining rats were involved in the establishment of the rat myocardial ischemia reperfusion injury model. The successfully established model was divided model group,Hexinshuang group,compound Danshen tablet group and Tianlong Tongxin tablet groups( 4,2,1 g·kg~(-1)). Nitrotetrazolium blue( N-BT) method was used to observe the alleviation of myocardial infarction. Colorimetry was used to detect the effect of the test drug on serum superoxide dismutase( SOD) and malondialdehyde( MDA). The Chandler in vitro method was used to detect thrombosis and blood viscosity in vitro of control group,Tianlong Tongxin tablets groups( 4,2,1 g·kg~(-1)),compound Danshen tablets group and aspirin group. The Born turbidimetric method was used to observe the platelet aggregation levels of control group,Tianlong Tongxin tablets groups( 2,1,0. 5 g·kg~(-1)),compound Danshen tablets group and aspirin group. Result:Compared with the sham operation group,the myocardial infarction area,serum SOD and MDA in the model group were significantly increased( P < 0. 05). Compared with the model group,Tianlong Tongxin tablets groups,Hexinshuang group and compound Danshen tablets group could reduce the degree of myocardial infarction,and the percentage of infarction area in the ventricle and heart decreased significantly( P < 0. 05,P < 0. 01). Compared with the control group,Tianlong Tongxin tablets groups( 4,2 g·kg~(-1)),compound Danshen tablets group and Aspirin tablets group could significantly shorten the length of thrombosis( P < 0. 05,P < 0. 01),and significantly reduce the wet weight and dry weight of thrombosis( P < 0. 05,P < 0. 01). In addition,the whole blood viscosities under 150,60 and 5 s~(-1) shear rates were significantly reduced( P < 0. 05,P < 0. 01). Compared with the control group,the platelet aggregation rates induced by collagen,adenosine bisphosphate( ADP) and arachidonic acid( AA) in rabbits were significantly reduced in Tianlong Tongxin tablet group( 2,1 g·kg~(-1)),compound Danshen tablet group and Aspirin tablet group( P < 0. 05,P < 0. 01). Conclusion: Tianlong Tongxin tablet can protect rat myocardial ischemia reperfusion injury,inhibit platelet aggregation and thrombosis,and reduce blood viscosity.
Objective: To explore the protective effect of Tianlong Tongxin tablet on myocardial ischemia reperfusion injury in rats,and observe its effect on thrombosis,blood viscosity and platelet aggregation in rabbits.Method: Totally 56 Wistar rats were collected. Except for the sham operation group,all of the remaining rats were involved in the establishment of the rat myocardial ischemia reperfusion injury model. The successfully established model was divided model group,Hexinshuang group,compound Danshen tablet group and Tianlong Tongxin tablet groups( 4,2,1 g·kg~(-1)). Nitrotetrazolium blue( N-BT) method was used to observe the alleviation of myocardial infarction. Colorimetry was used to detect the effect of the test drug on serum superoxide dismutase( SOD) and malondialdehyde( MDA). The Chandler in vitro method was used to detect thrombosis and blood viscosity in vitro of control group,Tianlong Tongxin tablets groups( 4,2,1 g·kg~(-1)),compound Danshen tablets group and aspirin group. The Born turbidimetric method was used to observe the platelet aggregation levels of control group,Tianlong Tongxin tablets groups( 2,1,0. 5 g·kg~(-1)),compound Danshen tablets group and aspirin group. Result:Compared with the sham operation group,the myocardial infarction area,serum SOD and MDA in the model group were significantly increased( P < 0. 05). Compared with the model group,Tianlong Tongxin tablets groups,Hexinshuang group and compound Danshen tablets group could reduce the degree of myocardial infarction,and the percentage of infarction area in the ventricle and heart decreased significantly( P < 0. 05,P < 0. 01). Compared with the control group,Tianlong Tongxin tablets groups( 4,2 g·kg~(-1)),compound Danshen tablets group and Aspirin tablets group could significantly shorten the length of thrombosis( P < 0. 05,P < 0. 01),and significantly reduce the wet weight and dry weight of thrombosis( P < 0. 05,P < 0. 01). In addition,the whole blood viscosities under 150,60 and 5 s~(-1) shear rates were significantly reduced( P < 0. 05,P < 0. 01). Compared with the control group,the platelet aggregation rates induced by collagen,adenosine bisphosphate( ADP) and arachidonic acid( AA) in rabbits were significantly reduced in Tianlong Tongxin tablet group( 2,1 g·kg~(-1)),compound Danshen tablet group and Aspirin tablet group( P < 0. 05,P < 0. 01). Conclusion: Tianlong Tongxin tablet can protect rat myocardial ischemia reperfusion injury,inhibit platelet aggregation and thrombosis,and reduce blood viscosity.
引文
[1]关双,苏苑苑,刘琼,等.基于网络药理学预测银杏蜜环口服溶液治疗冠心病与焦虑症的分子机制[J].中国实验方剂学杂志,2018,24(15):182-189.
[2]王薇.冠心病心绞痛中医证型分布及证型与客观指标相关性研究[D].广州:广州中医药大学,2013.
[3]林力,孙明谦,苗兰,等.中药复方天龙通心在大鼠体内的药代动力学研究[J].药学学报,2017,52(4):575-581.
[4] Zirpoli H,Abdillahi M,Quadri N,et al. Acute administration of n-3 rich triglyceride emulsions provides cardioprotection in murine models after ischemiareperfusion[J]. PLo S One,2015,10(1):e0116274.
[5]李峰杰,李贻奎,何萍,等.双龙方对大鼠心肌缺血/再灌注后无复流影响的研究[J].中国药理学通报,2018,34(4):583-588.
[6] Van Oeveren W1,Tielliu I F,de Hart J. Comparison of modified chandler, roller pump, and ball valve circulation models for in vitro testing in high blood flow conditions:application in thrombogenicity testing of different materials for vascular applications[J]. Int J Biomater,2015,doi:10. 1155/2012/673163.
[7] WANG L Z,WANG Z X,ZHANG F F,et al. Enhancing transgene expression from recombinant aav8 vectors in different tissues using woodchuck hepatitis virus posttranscriptional regulatory element[J]. Int J Med Sci,2016,13(4):286-291.
[8] Born G. Aggregation of blood platelets by adenosine diphosphate(adp)and its reversal[J]. Nature,1962,194(4832):927-929.
[9]黄志强,王媞媞,刘秀杰. 4-甲氧基-1,3-苯二磺酸酯类化合物的合成及其体外抗血小板聚集活性[J].中国药物化学杂志,2017,27(4):274-278.
[10]陈卓,李文杰,杨莺,等.双参通脉颗粒对心肌缺血再灌注大鼠NF-κB信号通路的影响[J].中国实验方剂学杂志,2018,24(13):136-140.
[11]王紫监,张立民,赵自刚,等.心肌缺血再灌注损伤研究进展[J].中国老年学杂志,2018,38(6):1532-1535.
[12]李磊,徐立,李振,等.芪龙胶囊对犬急性心肌缺血的影响[J].世界中医药,2018,13(1):31-35,40.
[13] Lilly leonard S.心血管病理生理学[M].北京:人民军医出版社,2013:213.
[14]王澍,张雪,马樱芮,等.补肾化痰益智方联合丁苯酞对阿尔茨海默病患者氧化应激、血流变学及认知功能的影响[J].中国实验方剂学杂志,2018,24(7):212-216.
[2]王薇.冠心病心绞痛中医证型分布及证型与客观指标相关性研究[D].广州:广州中医药大学,2013.
[3]林力,孙明谦,苗兰,等.中药复方天龙通心在大鼠体内的药代动力学研究[J].药学学报,2017,52(4):575-581.
[4] Zirpoli H,Abdillahi M,Quadri N,et al. Acute administration of n-3 rich triglyceride emulsions provides cardioprotection in murine models after ischemiareperfusion[J]. PLo S One,2015,10(1):e0116274.
[5]李峰杰,李贻奎,何萍,等.双龙方对大鼠心肌缺血/再灌注后无复流影响的研究[J].中国药理学通报,2018,34(4):583-588.
[6] Van Oeveren W1,Tielliu I F,de Hart J. Comparison of modified chandler, roller pump, and ball valve circulation models for in vitro testing in high blood flow conditions:application in thrombogenicity testing of different materials for vascular applications[J]. Int J Biomater,2015,doi:10. 1155/2012/673163.
[7] WANG L Z,WANG Z X,ZHANG F F,et al. Enhancing transgene expression from recombinant aav8 vectors in different tissues using woodchuck hepatitis virus posttranscriptional regulatory element[J]. Int J Med Sci,2016,13(4):286-291.
[8] Born G. Aggregation of blood platelets by adenosine diphosphate(adp)and its reversal[J]. Nature,1962,194(4832):927-929.
[9]黄志强,王媞媞,刘秀杰. 4-甲氧基-1,3-苯二磺酸酯类化合物的合成及其体外抗血小板聚集活性[J].中国药物化学杂志,2017,27(4):274-278.
[10]陈卓,李文杰,杨莺,等.双参通脉颗粒对心肌缺血再灌注大鼠NF-κB信号通路的影响[J].中国实验方剂学杂志,2018,24(13):136-140.
[11]王紫监,张立民,赵自刚,等.心肌缺血再灌注损伤研究进展[J].中国老年学杂志,2018,38(6):1532-1535.
[12]李磊,徐立,李振,等.芪龙胶囊对犬急性心肌缺血的影响[J].世界中医药,2018,13(1):31-35,40.
[13] Lilly leonard S.心血管病理生理学[M].北京:人民军医出版社,2013:213.
[14]王澍,张雪,马樱芮,等.补肾化痰益智方联合丁苯酞对阿尔茨海默病患者氧化应激、血流变学及认知功能的影响[J].中国实验方剂学杂志,2018,24(7):212-216.
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