痛风二号方对湿热型高尿酸血症大鼠血清肿瘤坏死因子α和血管活性肠肽水平的影响研究
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摘要
目的:研究湿热型高尿酸血症(HUA)大鼠血尿酸、肿瘤坏死因子α(Tnf-α)和血管活性肠肽(VIP)的变化,以及痛风二号方对HUA大鼠血清尿酸、肿瘤坏死因子α、血管活性肠肽的影响。方法:将40只Wistar雄性大鼠,随机分为空白对照组、湿热高尿酸血症模型组(模型组)、别嘌醇组、痛风二号方组。空白对照组:正常喂饲,在其余三组进行模型建立和药物干预的时候予等量生理盐水行灌胃、腹腔注射等相应操作。湿热型高尿酸血症模型组:高脂高糖饮食、白酒灌胃、200g/L蜂蜜水自由饮用,持续14天。自第八天开始,酵母膏按比例混入高脂饲料喂饲联合腹腔注射氧嗪酸钾,制备大鼠模型;同时自第10天开始,将大鼠置入湿热环境中放置4天。痛风二号方组,别嘌醇组:造模同湿热型高尿酸血症模型组,自造模第八天起,灌胃及注射造模药物后6小时,分别给予大鼠痛风二号方(0.3g/kg)或别嘌醇(40mg/kg)灌胃干预,直到第14天。生化分析仪检测大鼠血清尿酸值;通过ELISA法检测大鼠血清VIP、Tnf-α。结果:模型组、两药物干预组(别嘌醇组、痛风二号方组)血尿酸值、血清Tnf-α、血清VIP值与空白对照组相比,差异均具有显著性统计学意义(P<0.01),痛风二号方组血尿酸值、血清Tnf-α、血清VIP值与模型组相比差异具有显著性统计学意义(P<0.01),别嘌醇组与模型组血尿酸值、血清Tnf-α差异具有显著性统计学意义(P<0.01或P<0.05),血清VIP差异无统计学意义(P>0.05),两药物干预组之间血尿酸值、血清Tnf-α、血清VIP值差异具有显著统计学意义(P<0.01或P<0.05)。结论:痛风二号方对湿热型高尿酸血症有明显的治疗作用,在降尿酸的同时还可降低Tnf-α及升高VIP,且疗效优于别嘌醇,对湿热型高尿酸血症有较好的防治作用。
Objective:To study the changes of serum uric acid,tumor necrosis factor-α(Tnf-α) and vasoactive intestinal peptide(VIP) in(HUA) rats with hyperuricemia of damp-heat type,and the effect of Tongfeng No.2 Recipe on serum uric acid and Tnf-αin HUA rats,and effect of vasoactive intestinal peptides.Methods:Forty male Wistar rats were randomly divided into blank controlled group,model group with hyperuricemia of dampness and heat,allopurinol group and Tongfeng No.2 Recipe group.The blank controlled group:normal feeding,the other three groups of model and drug intervention with the same amount of normal saline,intraperitoneal injection and other corresponding operations.Hygrothermal hyperurination acidemia model group:high fat and high sugar diet,liquor by stomach,200 g/L honey water for 14 days.From the eighth day,the rats were fed with high fat diet and intraperitoneal injection of potassium oxazinate,and then placed in humid and hot environment for 4 days.Tongfeng No.2 Recipe group,allopurinol group:the model group of hyperuricemia of the same damp-heat type,from the eighth day of the model,6 hours after intragastric administration and injection of the drug to make the model.Rats were given Tongfeng No.2 Recipe(0.3 g/kg) or allopurinol(40 mg/1 kg) respectively,until the Fourteenth days.Serum uric acid was measured by biochemical analyzer and serum VIP,Tnf-αwas detected by ELISA method.Results:There were significant differences in serum uric acid,serum Tnf-α and serum VIP between the two drug intervention groups(the allopurinol group,the Tongfeng No.2 Recipe group) compared with the blank controlled group(P<0.01).The levels of serum uric acid,serum Tnf-α and serum VIP in Tongfeng No.2 Recipe group were significantly different from those in the model group(P<0.01),and the serum uric acid value in the allopurinol group and the model group was significantly higher than that in the model group(P<0.01).There was significant difference in serum Tnf-α(P<0.01 or P<0.05),but no significant difference in serum VIP(P>0.05) there were significant differences in serum uric acid,serum Tnf-αand serum VIP between the two drug intervention groups(P<0.01 or P<0.05).Conclusion:Tongfeng No.2 Recipe has obvious therapeutic effect on hygrothermal hyperuricemia.It can reduce Tnf-αand increase VIP,at the same time,and the curative effect is better than allopurinol.It has better preventive and therapeutic effect on hygrothermal hyperuricemia.
Objective:To study the changes of serum uric acid,tumor necrosis factor-α(Tnf-α) and vasoactive intestinal peptide(VIP) in(HUA) rats with hyperuricemia of damp-heat type,and the effect of Tongfeng No.2 Recipe on serum uric acid and Tnf-αin HUA rats,and effect of vasoactive intestinal peptides.Methods:Forty male Wistar rats were randomly divided into blank controlled group,model group with hyperuricemia of dampness and heat,allopurinol group and Tongfeng No.2 Recipe group.The blank controlled group:normal feeding,the other three groups of model and drug intervention with the same amount of normal saline,intraperitoneal injection and other corresponding operations.Hygrothermal hyperurination acidemia model group:high fat and high sugar diet,liquor by stomach,200 g/L honey water for 14 days.From the eighth day,the rats were fed with high fat diet and intraperitoneal injection of potassium oxazinate,and then placed in humid and hot environment for 4 days.Tongfeng No.2 Recipe group,allopurinol group:the model group of hyperuricemia of the same damp-heat type,from the eighth day of the model,6 hours after intragastric administration and injection of the drug to make the model.Rats were given Tongfeng No.2 Recipe(0.3 g/kg) or allopurinol(40 mg/1 kg) respectively,until the Fourteenth days.Serum uric acid was measured by biochemical analyzer and serum VIP,Tnf-αwas detected by ELISA method.Results:There were significant differences in serum uric acid,serum Tnf-α and serum VIP between the two drug intervention groups(the allopurinol group,the Tongfeng No.2 Recipe group) compared with the blank controlled group(P<0.01).The levels of serum uric acid,serum Tnf-α and serum VIP in Tongfeng No.2 Recipe group were significantly different from those in the model group(P<0.01),and the serum uric acid value in the allopurinol group and the model group was significantly higher than that in the model group(P<0.01).There was significant difference in serum Tnf-α(P<0.01 or P<0.05),but no significant difference in serum VIP(P>0.05) there were significant differences in serum uric acid,serum Tnf-αand serum VIP between the two drug intervention groups(P<0.01 or P<0.05).Conclusion:Tongfeng No.2 Recipe has obvious therapeutic effect on hygrothermal hyperuricemia.It can reduce Tnf-αand increase VIP,at the same time,and the curative effect is better than allopurinol.It has better preventive and therapeutic effect on hygrothermal hyperuricemia.
引文
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[12]Chichanghua GN,Stankulov IS,Hristova M,et al.Adipobiology of disease:adipokines and adipokine-targeted pharmacology[J].Curr Pharm Des,2013,9(12):1023~1031
[13]李红林,高美华,郑云会,等.细胞因子IFN-γ、IL-6、IL-17和TGF-β1在Graves病发病中的作用[J].中国免疫学杂志,2015,31(02):253~256
[2]雷三霞,于长国,曾春艳.高尿酸血症与肾损害相关性研究进展[J].医学综述,2014,(10):1775~1777
[3]朱明敏.复方土茯苓颗粒对高尿酸血症黄嘌呤氧化酶的抑制作用[D].广州中医药大学,2015
[4]NakagawaT,Hu H,ZharikovS,etal.Acausalroleforuricacidinfructose-inducedmetabolicsyndrome[J].Am J Physiol Real Physiol,2006,290(3):F625~F631
[5]陈红艳,邢玉娥.高脂血症、高血压与高尿酸血症的相关性分析[J].现代预防医学,2011,38(02):359~360
[6]李争.心血管健康行为和因素与高尿酸血症[D].天津:天津医科大学,2016
[7]王红坡.菊苣降尿酸有效成分的血清药物化学研究[D].北京:北京中医药大学,2016
[8]胡玲,崔娜娟,罗琦,等.慢性胃炎脾胃湿热证与热休克蛋白70和核因子-κB炎症通路表达的关系[J].广州中医药大学学报,2010,27(06):587~591,669~670
[9]吕冠华,劳绍贤.脾胃湿热证动物模型的建立与评价[J].广州中医药大学学报,2005,(03):231~235
[10]单瑞,程爱娟,吴尚勤.苯溴马隆与别嘌醇对心力衰竭伴高尿酸血症患者炎症因子及心功能的影响[J].中华高血压杂志,2014,22(04):380~383
[11]胡玲,崔娜娟,罗琦,等.慢性胃炎脾胃湿热证与热休克蛋白70和核因子-κB炎症通路表达的关系[J].广州中医药大学学报,2010,27(06):587~591,669~670
[12]Chichanghua GN,Stankulov IS,Hristova M,et al.Adipobiology of disease:adipokines and adipokine-targeted pharmacology[J].Curr Pharm Des,2013,9(12):1023~1031
[13]李红林,高美华,郑云会,等.细胞因子IFN-γ、IL-6、IL-17和TGF-β1在Graves病发病中的作用[J].中国免疫学杂志,2015,31(02):253~256