吲哚-2,3-二酮对醋酸棉酚致生精障碍小鼠的影响
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摘要
目的观察吲哚-2,3-二酮对醋酸棉酚致雄性小鼠生精障碍模型生精功能的影响。方法雄性昆明种小鼠分为正常对照组、模型对照组和吲哚-2,3-二酮25、50、100 mg·kg~(-1)组(n=8)。醋酸棉酚20 mg·kg~(-1)灌胃30 d制备生精障碍模型。制模同时吲哚-2,3-二酮各剂量组灌胃给予相应剂量30 d后,观察小鼠精子、睾丸发育及生育的影响。结果与空白对照组比较,模型对照组精子密度和活动率均降低(P<0.01),精原细胞、初级精母细胞及精子细胞数目减少(P<0.01);吲哚-2,3-二酮25、50、100 mg·kg~(-1)组精子密度和活动率显著高于模型对照组(P<0.05或P<0.01),吲哚-2,3-二酮25 mg·kg~(-1)组初级精母细胞数目增多(P<0.05),50 mg·kg~(-1)、100 mg·kg~(-1)组精原细胞、初级精母细胞及精子细胞数目均增多(P<0.01),呈剂量依赖性。模型对照组孕鼠1只,胎鼠3只;吲哚-2,3-二酮25 mg·kg~(-1)、50 mg·kg~(-1)和100 mg·kg~(-1)组孕鼠分别为2、3、6只,胎鼠分别为(6.1±2.1)、(7.5±1.7)和(8.6±1.4)只。结论吲哚-2,3-二酮可改善醋酸棉酚致雄性小鼠生精障碍模型的生精功能。
AIM To investigate the effects of indole-2, 3-dione on the spermatogenesis impairment model induced by gossypol acetate on male mice. METHODS The male Kunming mice were divided into normal control group, model control group and indole-2, 3-dione 25, 50, 100 mg·kg~(-1) groups according to random digits table( n = 8). Except the normal control group, other groups were intragastricly administrated with gossypol acetate 20 mg·kg~(-1) for 30 days to establish the spermatogenesis impairment model. Normal saline was given to mice in the normal and model control groups. The mice were given the corresponding dose of indole-2,3-dione for 30 days. Sperm, testicular development and fertility were observed. RESULTS Compared with the normal control group, the sperm density and activity rate were reduced in the model control group(P < 0.01).And the numbers of spermatogonia, primary spermatocytes and sperm cells were also reduced(P < 0.01). The sperm density and motility in the indole-2, 3-dione 25, 50, 100 mg·kg~(-1) groups were significantly higher than that in the model control group(P < 0.05 or P < 0.01). The numbers of spermatocytes in the indole-2, 3-dione25 mg·kg~(-1) group were increased(P < 0.05). The numbers of spermatogonia, primary spermatocytes and sperm cells were increased in the indole-2, 3-dione 50, 100 mg·kg~(-1) groups(P < 0.01), and the increase was dose dependent. After paired with females, the fetal mice were(12.7 ± 1.5) in the normal control group. There were1 pregnant mouse and 3 fetal mice in the model control group. The pregnant mice were 2, 3 and 6 and the fetal mice were( 6.1 ± 2.1),( 7.5 ± 1.7) and( 8.6 ± 1.4) in the indole-2, 3-dione 25, 50 and 100 mg·kg~(-1) groups, respectively. CONCLUSION Indole-2, 3-dione can improve the spermatogenic function of the spermatogenesis impairment male mice induced by gossypol acetate.
AIM To investigate the effects of indole-2, 3-dione on the spermatogenesis impairment model induced by gossypol acetate on male mice. METHODS The male Kunming mice were divided into normal control group, model control group and indole-2, 3-dione 25, 50, 100 mg·kg~(-1) groups according to random digits table( n = 8). Except the normal control group, other groups were intragastricly administrated with gossypol acetate 20 mg·kg~(-1) for 30 days to establish the spermatogenesis impairment model. Normal saline was given to mice in the normal and model control groups. The mice were given the corresponding dose of indole-2,3-dione for 30 days. Sperm, testicular development and fertility were observed. RESULTS Compared with the normal control group, the sperm density and activity rate were reduced in the model control group(P < 0.01).And the numbers of spermatogonia, primary spermatocytes and sperm cells were also reduced(P < 0.01). The sperm density and motility in the indole-2, 3-dione 25, 50, 100 mg·kg~(-1) groups were significantly higher than that in the model control group(P < 0.05 or P < 0.01). The numbers of spermatocytes in the indole-2, 3-dione25 mg·kg~(-1) group were increased(P < 0.05). The numbers of spermatogonia, primary spermatocytes and sperm cells were increased in the indole-2, 3-dione 50, 100 mg·kg~(-1) groups(P < 0.01), and the increase was dose dependent. After paired with females, the fetal mice were(12.7 ± 1.5) in the normal control group. There were1 pregnant mouse and 3 fetal mice in the model control group. The pregnant mice were 2, 3 and 6 and the fetal mice were( 6.1 ± 2.1),( 7.5 ± 1.7) and( 8.6 ± 1.4) in the indole-2, 3-dione 25, 50 and 100 mg·kg~(-1) groups, respectively. CONCLUSION Indole-2, 3-dione can improve the spermatogenic function of the spermatogenesis impairment male mice induced by gossypol acetate.
引文
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[16]岳旺,李丰桥,安部阳一.内源性MAO-B抑制因子-靛红预防MPTP对多巴胺神经递质的耗竭作用[J].中国神经科学杂志,2002,18(4):690-693.
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[19]杨杰,毛晓红,张华,等.HPLC法测人体精液中2,3-吲哚醌的含量[J].泰山医学院学报,2013,34(1):12-13.
[20]王蕾,徐宗荣,李静,等.2,3-吲哚醌对大鼠脑内单胺类神经递质含量与释放的影响[J].中国海洋药物杂志,2005,24(4):28-30.
[21]REN A,SU B,YE S,et al.A pharmacokinetic study of Isatin in Beagles'bodies[J].Exp Ther Med,2016,11(6):2225-2228.
[2]王蕾,耿波,申红霞,等.2,3-吲哚醌的抗衰老作用及机制研究[J].中国行为医学科学,2007,16(6):481-483.
[3]陈达,孟晶晶,张芳,等.海洋抗生素吲哚-2,3-二酮的抗病毒作用[J].中国海洋药物杂志,2006,25(4):30-33.
[4]王蕾,曹静,鞠传霞,等.2,3-吲哚醌抗肿瘤作用研究[J].中国药理学通报,2007,23(8):1052-1056.
[5]殷津津,宋丽阳,杨杰.吲哚-2,3-二酮抗菌作用研究[J].泰山医学院学报,2013,34(2):107-109.
[6]GLOVER V,BHATTACHARYA SK,SANDLER M.Isatin-a new biological factor[J].Indian J Exp Biol,1991,29(1):1-5.
[7]郭春丽,徐辉辉,师淑君,等.内源性活性物质2,3-吲哚醌抗前列腺增生作用[J].医药导报,2018,37(5):509-511.
[8]倪崖,陈文颖,李春波,等.补肾强精颗粒剂的药效学研究[J].中国现代应用药学杂志,2006,23(4):268-271.
[9]GLOVER V,HALKET JM,WATKINS PJ,et al.Isatin:identity with the purified endogenous monoamine oxidase inhibitor tribulin[J].J Neurochem,1988,51(2):656-659.
[10]岳旺,石振艳,杨志宏,等.吲哚-2,3-二酮在制备抗精子畸形药物中的应用[P].中国专利,ZL 201110132551.X.
[11]李建军,温利锋,张建梅,等.长沙地区男性不育相关因素流行病学分析[J].中国妇幼保健,2016,31(24):5466-5470.
[12]姚志文,吴晓宇,林巧雅.环境激素与生殖健康[J].中国优生与遗传杂志,2001,9(1):12-13.
[13]HOSOE T,NOZAWA K,KAWAHARA N,et al.Isolation of a new potent cytotoxic pigment along with indigotin from the pathogenic basidiomycetous fungus Schizophyllum commune[J].Mycopathologia,1999,146(1):9-12.
[14]GIL-TURNES MS,HAY ME,FENICAL W.Symbiotic marine bacteria chemically defend crustacean embryos from a pathogenic fungus[J].Science,1989,246(4926):l16-118.
[15]刘占涛,张健,王蕾,等.内源性MAO-B抑制因子靛红对大鼠旋转行为的影响[J].中国海洋药物,2001,38(5):11-13.
[16]岳旺,李丰桥,安部阳一.内源性MAO-B抑制因子-靛红预防MPTP对多巴胺神经递质的耗竭作用[J].中国神经科学杂志,2002,18(4):690-693.
[17]SUSHEELA I,SINGH B,DANI HM,et al.Enzyme inhibition by isatin I.Inhibition of rat liver xanthine oxidase[J].Enzymologia,1969,37(5):325-334.
[18]MEDVEDEV AE,CLOW A,SANDLER M,et al.Isatin:a link between natriuretic peptides and monoamines[J].Biochem Pharmacol,1996,52(3):385-391.
[19]杨杰,毛晓红,张华,等.HPLC法测人体精液中2,3-吲哚醌的含量[J].泰山医学院学报,2013,34(1):12-13.
[20]王蕾,徐宗荣,李静,等.2,3-吲哚醌对大鼠脑内单胺类神经递质含量与释放的影响[J].中国海洋药物杂志,2005,24(4):28-30.
[21]REN A,SU B,YE S,et al.A pharmacokinetic study of Isatin in Beagles'bodies[J].Exp Ther Med,2016,11(6):2225-2228.