猪源抗菌肽对禽源沙门氏菌和大肠杆菌的抑菌作用
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摘要
【目的】明确猪源抗菌肽对禽源沙门氏菌和大肠杆菌的抑菌作用,为新型抗菌药物的研发和猪源抗菌肽的生产应用提供科学依据。【方法】以猪源抗菌肽为材料,采用液体培养基抑制测定法,结合体内外试验,分别测定猪源抗菌肽对禽源沙门氏菌和禽源大肠杆菌的抗菌活力值,研究其体内外抑菌作用。【结果】对不同浓度的抗菌肽抗菌活力值的测定结果表明,与0.5、0.2和0.1mg·mL~(-1)的猪源抗菌肽相比较,1.0mg·mL~(-1)的抗菌肽对含有106 CFU·mL~(-1)的菌液抑菌效果最好;与阿莫西林、红霉素、四环素等15种抗生素相比较,猪源抗菌肽对禽源沙门氏菌FJS13株和禽源大肠杆菌FJE61株的抑菌圈最大,其抗菌效果最好;抗菌肽在雏鸡体内的抑菌试验表明,攻毒鸡皮下注射猪源抗菌肽有利于提高雏鸡的存活率,在雏鸡体内也具有一定的抑菌作用。【结论】猪源抗菌肽可用于抑制禽源沙门氏菌和大肠杆菌。
【Objective】Activity of porcine antibacterial peptides(PAP)against avian Salmonellaand Escherichia coli was determined for drug development.【Method】Inhibitive activity of PAP on avian pathogens was studied in vitro on an liquid medium and by an in vivo test.【Results】PAP at a concentration of 1.0 mg·mL~(-1) showed a higher bacteriostatic effect on the solution contaning 10~6 CFU·mL~(-1) of the pathogens than at 0.5,0.2 or 0.1 mg·mL~(-1).Compared with 15 antibiotics,such as amoxicillin,erythromycin,tetracycline,etc.,PAP induced the largest inhibition zone on the growth of avian Salmonella FJS13 and E.coli FJE61.In a challenging test on chicken,the PAP administration improved the survival rate of the subcutaneous injected birds.【Conclusion】PAP could be used as a bioagent to combat the infection on chickens by avian Salmonellaand E.coli.
【Objective】Activity of porcine antibacterial peptides(PAP)against avian Salmonellaand Escherichia coli was determined for drug development.【Method】Inhibitive activity of PAP on avian pathogens was studied in vitro on an liquid medium and by an in vivo test.【Results】PAP at a concentration of 1.0 mg·mL~(-1) showed a higher bacteriostatic effect on the solution contaning 10~6 CFU·mL~(-1) of the pathogens than at 0.5,0.2 or 0.1 mg·mL~(-1).Compared with 15 antibiotics,such as amoxicillin,erythromycin,tetracycline,etc.,PAP induced the largest inhibition zone on the growth of avian Salmonella FJS13 and E.coli FJE61.In a challenging test on chicken,the PAP administration improved the survival rate of the subcutaneous injected birds.【Conclusion】PAP could be used as a bioagent to combat the infection on chickens by avian Salmonellaand E.coli.
引文
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[15]朱健,刘超群,刘瑞奇,等.抗菌肽免疫调节功能的研究进展[J].黑龙江畜牧兽医.2018,24(1):77-79.ZHU J,LIU C Q,LIU R Q,et al.Research progress on immunomodulatory function of antimicrobial peptides[J].Heilongjiang Animal Husbandry and Veterinary.2018,24(1):77-79.(in Chinese)
[16]卜艳玲.肠杆菌肽体外抑菌作用及在断奶仔猪生产中应用研究[D].沈阳:沈阳农业大学,2018.BU Y L.In vitro antibacterial effect of enterobacterin and its application in weaned pig production[D].Shenyang:Shenyang Agricultural University,2018.(in Chinese)
[2]魏中琴,高雅婷,马奔科,等.抗菌肽的研究进展[J].黑龙江医药,2015,10(1):1-5.WEI Z Q,GAO Y T,MA B K,et al.Research progress of antimicrobial peptides[J].Heilongjiang Medicine,2015,10(1):1-5.(in Chinese)
[3]韩新燕,汪以真,许梓荣.哺乳动物抗菌肽的研究进展[J].中国兽医学报,2002,15(2):205-208.HAN X Y,WANG Y Z,XU Z R.Research progress of mammalian antimicrobial peptides[J].Chinese Journal of Veterinary Medicine.2002,15(2):205-208.(in Chinese)
[4]ANDREAS R KOCZULLA.Robert Bals.Antimicrobial Peptides:current status and therapeutic potential[J].Drugs,2003,63(4):389-460.
[5]马卫明,佘锐萍,彭芳珍,等.猪小肠抗菌肽的提取及部分生物学活性研究[J].科学技术与工程,2004,16(3):202-205.MA W M,QI R P,PENG F Z,et al.Extraction and Biological Activity of Porcine Small Intestinal Antibacterial Peptides[J].Science Technology and Engineering,2004,16(3):202-205.(in Chinese)
[6]范志勇,周定刚,吴明夏,等.猪小肠抗菌肽的抑菌作用研究[J].家畜生态学报,2006,27(6):99-101.FAN Z Y,ZHOU D G,WU M X,et al.Antibacterial activity of porcine intestinal antimicrobial peptides[J].Chinese Journal of Animal Ecology,2006,27(6):99-101.(in Chinese)
[7]高明燕,刘爱玲,刘建军,等.抗菌肽RP-07的抑菌作用及其稳定性研究[J].黑龙江畜牧兽医,2015(7):189-191.GAO M Y,LIU A L,LIU J J,et al.Antibacterial activity and stability of antimicrobial peptide RP-07[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2015(7):189-191.(in Chinese)
[8]POWERS J P,HANCOCK R E.The relationship between peptide structure and antibacterial activity[J].Peptides,2003,24(11):1681-1691.
[9]单安山,田昊天,邵长轩,等.抗菌肽抗细菌机理研究进展[J].东北农业大学学报,2018,8(3):84-94.SHAN A S,TIAN H T,SHAO C X,et al.Advances in antibacterial mechanism of antibacterial peptides[J].Journal of Northeast Agricultural University,2018,8(3):84-94.(in Chinese)
[10]MOHAN K V.Antiviral activity of selected antimicrobial peptides against vaccinia virus[J].Antiviral Research,2010,86(3):306-311.
[11]BRADSHAW JEREMY.Cationic antimicrobial peptides:issues for potential clinical use[J].Bio Drugs,clinicalimmunotherapeutics,biopharmaceuticals and gene therapy,2003,17(4):233-240.
[12]徐磊,刘友霖,曾辉荣,等.猪小肠复合抗菌肽的制备方法及用途:中国,201310343030.8[P].2015-08-11.XU L,LIU Y L,ZENG H R,et al.Preparation method and use of pig small intestine compound antimicrobial peptide:China,201310343030.8[P].2015-08-11.(in Chinese)
[13]Clinical and Laboratory Standards Institute(CLSI).Performance standards for antimicrobial susceptibility testing twentieth informational supplement[M].USA,2010.
[14]董改琳.家禽大肠杆菌致病机理与防治措施[J].畜牧兽医科学,2018,5(4):64.DONG G L.Pathogenic Mechanism and Control Measures of Escherichia Coli in Poultry[J].Journal of Animal Husbandry and Veterinary Medicine,2018,5(4):64.(in Chinese)
[15]朱健,刘超群,刘瑞奇,等.抗菌肽免疫调节功能的研究进展[J].黑龙江畜牧兽医.2018,24(1):77-79.ZHU J,LIU C Q,LIU R Q,et al.Research progress on immunomodulatory function of antimicrobial peptides[J].Heilongjiang Animal Husbandry and Veterinary.2018,24(1):77-79.(in Chinese)
[16]卜艳玲.肠杆菌肽体外抑菌作用及在断奶仔猪生产中应用研究[D].沈阳:沈阳农业大学,2018.BU Y L.In vitro antibacterial effect of enterobacterin and its application in weaned pig production[D].Shenyang:Shenyang Agricultural University,2018.(in Chinese)
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