噻呋酰胺对河南省小麦纹枯病菌的抑制活性
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摘要
为明确噻呋酰胺对河南省小麦纹枯病菌的抑制活性,采用菌丝生长速率法测定了噻呋酰胺对2016年从河南省15个地市分离的98株病菌的毒力。结果表明,供试菌株对噻呋酰胺的EC_(50)值分布于0.009～0.405μg·mL~(-1),小麦纹枯病菌群体中存在对噻呋酰胺敏感性较低的亚群体,将其中呈连续单峰频次正态分布的大多数菌株的平均EC_(50)值(0.043±0.019μg·mL~(-1))作为病菌对噻呋酰胺的敏感性基线;方差分析及聚类分析显示,同一地市内的菌株对噻呋酰胺的敏感性(即EC_(50)值)差异较大,EC_(50)最大值和最小值之比为1.78～34.89,而不同地市的菌株对噻呋酰胺的敏感性差异不明显,噻呋酰胺EC_(50)平均值为0.035～0.155μg·mL~(-1),EC_(50)最大值是最小值的4.41倍,病菌对噻呋酰胺的敏感性与菌株的地理来源无明显相关性;噻呋酰胺对病菌的毒力与苯醚甲环唑、戊唑醇、井冈霉素及甲基立枯磷的毒力间无明显相关性。综上所述,河南省大部分小麦纹枯病菌菌株对噻呋酰胺较为敏感,可将其作为综合防控的备选药剂;生产中可将噻呋酰胺与苯醚甲环唑、戊唑醇、甲基立枯磷、井冈霉素4种药剂轮换或交替使用,以延长其使用寿命。
The objectives of the study is to verifythe sensitivities of Rhizoctonia cerealis in Henan province to thifluzamide and to screen the fungicide used to control wheat sharp eyespot. The sensitivities of R. cerealis to thifluzamide was determined by measuring the mycelial growth on the fungicide-amended media using 98 isolates collected from 15 cities of Henan province in 2016. 50% effective concentration(EC_(50)) values of all isolates to thifluzamide ranged from 0.0091 to 0.405 μg·mL~(-1),respectively. The results of the frequency analysis revealed that subpopulation,which was less sensitive to thifluzamide,were discovered in the tested isolates. The mean EC_(50) values of(0.043±0.019)μg·mL~(-1) for most isolates showed a unimodal curve distribution,which was treated as the sensitive baseline of R. cerealis to thifluzamide. The results of both variance analysis by LSD test and hierarchical cluster analysis by SPSS indicated that the isolates from the same region demonstrated different sensitivities,which implied that the ratio of maximum and minimum of EC_(50)values of isolates from the same region ranged from 1.78 to 34.89.However,there was no significant difference in the sensitivity to thifluzamide among the isolates from most of the regions,which showed that the average EC_(50)value of the isolates from all the regions ranged from 0.035 to 0.155 μg·mL~(-1) and the ratio ofmaximum tominimum of average EC_(50) value was 4.41. The isolates collected from different regions demonstrated different sensitivities. Especially,the correlation efficiencyexisted between thifluzamide and propiconazole,tebuconazole,difenoconazole,jinggangmycin and tolclofos-methyl was low. Most of the isolates of R. cerealis from Henan province were sensitive to thifluzamide and the fungicide canbe used to control wheat sharp eyespot as a replacement.In the field,tebuconazole,difenoconazole,jinggangmycin and tolclofos-methyl could be used alternatively with thifluzamide to prolong the lifetime.
The objectives of the study is to verifythe sensitivities of Rhizoctonia cerealis in Henan province to thifluzamide and to screen the fungicide used to control wheat sharp eyespot. The sensitivities of R. cerealis to thifluzamide was determined by measuring the mycelial growth on the fungicide-amended media using 98 isolates collected from 15 cities of Henan province in 2016. 50% effective concentration(EC_(50)) values of all isolates to thifluzamide ranged from 0.0091 to 0.405 μg·mL~(-1),respectively. The results of the frequency analysis revealed that subpopulation,which was less sensitive to thifluzamide,were discovered in the tested isolates. The mean EC_(50) values of(0.043±0.019)μg·mL~(-1) for most isolates showed a unimodal curve distribution,which was treated as the sensitive baseline of R. cerealis to thifluzamide. The results of both variance analysis by LSD test and hierarchical cluster analysis by SPSS indicated that the isolates from the same region demonstrated different sensitivities,which implied that the ratio of maximum and minimum of EC_(50)values of isolates from the same region ranged from 1.78 to 34.89.However,there was no significant difference in the sensitivity to thifluzamide among the isolates from most of the regions,which showed that the average EC_(50)value of the isolates from all the regions ranged from 0.035 to 0.155 μg·mL~(-1) and the ratio ofmaximum tominimum of average EC_(50) value was 4.41. The isolates collected from different regions demonstrated different sensitivities. Especially,the correlation efficiencyexisted between thifluzamide and propiconazole,tebuconazole,difenoconazole,jinggangmycin and tolclofos-methyl was low. Most of the isolates of R. cerealis from Henan province were sensitive to thifluzamide and the fungicide canbe used to control wheat sharp eyespot as a replacement.In the field,tebuconazole,difenoconazole,jinggangmycin and tolclofos-methyl could be used alternatively with thifluzamide to prolong the lifetime.
引文
[1]陈莹,李伟,张晓祥,等.中国北纬33度地区小麦纹枯病菌的群体组成及致病力研究[J].麦类作物学报,2009,29(6):1111. CHEN Y,LI W,ZHANG X X,et al.Composition and virulence of pathogen of wheat sharp eyespot in north latitude 33°of China [J].Journal of Triticeae Crops,2009,29(6):1111.
[2]HAMADA M S,YIN Y N,CHEN H G,et al.The escalating threat of Rhizoctonia cerealis ,the causal agent of sharp eyespot in wheat [J].Pest Management Science,2011,67(11):1411.
[3]蒋彦婕,吴纪中,蔡士宾,等.小麦抗纹枯病种质资源的鉴定与评价[J].麦类作物学报,2013,33(3):590. JIANG M J,WU J Z,CAI S B,et al.Screening of resistance to sharp eyespot in wheat germplasm [J].Journal of Triticeae Crops,2013,33(3):590.
[4]徐建强,伍士郎,赵建江,等.河南省小麦纹枯病菌对三唑酮的敏感性[J].植物病理学报,2017,47(1):138. XU J Q,WU S L,ZHAO J J,et al.Sensitivity of Rhizoctonia cerealis to triadimefon in Henan Province [J].Acta Phytopathologica Sinica,2017,47(1):138.
[5]徐建强,刁兴旺,李恒,等.中国河南省小麦纹枯病菌对苯醚甲环唑及戊唑醇的敏感性[J].农药学学报,2016,18(5):583. XU J Q,DIAO X W,LI H,et al.Sensitivity to difenoconazole and tebuconazole of Rhizoctonia cerealis in Henan Province in China [J].Chinese Journal of Pesticide Science,2016,18(5):583.
[6]仇是胜,柏亚罗.琥珀酸脱氢酶抑制剂类杀菌剂的研发进展(Ⅰ)[J].现代农药,2014,13(6):2. QIU S S,BAI Y L.Progress on research and development of succinate dehydrogenase inhibitor fungicides(Ⅰ) [J].Modern Agrochemicals,2014,13(6):2.
[7]宋立妹,张军民.小麦纹枯病菌对氟酰胺的敏感性[J].麦类作物学报,2013,33(6):1288. SONG L M, ZHANG J M.Sensitivity of Rhizoctonia cerealis to flutolanil [J].Journal of Triticeae Crops,2013,33(6):1288.
[8]仇是胜,柏亚罗.琥珀酸脱氢酶抑制剂类杀菌剂的研发进展(Ⅱ)[J].现代农药,2015,14(1):2. QIU S S,BAI Y L.Progress on research and development of succinate dehydrogenase inhibitor fungicides(Ⅱ) [J].Modern Agrochemicals,2015,14(1):2.
[9]魏松红,王海宁,胡吉祥,等.吉林地区水稻纹枯病菌对噻呋酰胺药物的敏感基线探讨[J].沈阳农业大学学报,2016,47(3):282. WEI S H,WANG H N,HU J X,et al.Sensitive baseline of Rhizoctonia solani to thifluzamide in Jilin Province [J].Journal of Shenyang Agricultural University,2016,47(3):282.
[10]中华人民共和国农业部农药检定所.农药登记数据:作物名称:水稻,小麦;有效成分Ⅰ—噻呋酰胺[EB/OL].中国农药信息网http://www.chinapesticide.gov.cn/hysj/index.jhtml,2017-7-7/2017-7-7. Pesticide Examimnnation Institute of the Ministry of Agriculture of the People's Repulic of China.Pesticide Registration Date,Crop:Rice or Wheat,Active Ingredient,Thifluzamide [EB/OL].China pesticide information network,http://www.chinapesticide.gov.cn/hysj/index.jhtml,2017-7-7/2017-7-7.
[11]齐永志,李海燕,苏媛,等.小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状[J].农药学学报,2014,16(3):274. QI Y Z,LI H Y,SU Y,et al.Sensitivity to trifluzamide and main biological characteristics of resistant mutants of Rhizoctonia cerealis [J].Chinese Journal of Pesticide Science,2014,16(3):274.
[12]SUN H Y,WANG C F,LI W,et al.Characterization of Rhizoctonia cerealis sensitivity to thifluzamide in China [J].Crop Protection,2015,69(3):67.
[13]ZHANG Y,LU J L,WANG J X,et al.Baseline sensitivity and resistance risk assessmemt of Rhizoctonia cerealis to thifluzamide,a succinate dehydrogenase inhibitor [J].Pesticide Biochemistry and Physiology,2015,124(10):99.
[14]任学祥,叶正和,丁克坚,等.噻呋酰胺种衣剂防治小麦纹枯病效果及安全性研究[J].麦类作物学报,2015,35(11):1590-1591. REN X X,YE Z H,DING K J,et al.Effect of seed coating treatments with thifluzamide on wheat sharp eyespot and safety evaluation [J].Journal of Triticeae Crops,2015,35(11):1590.
[15]徐建强,平忠良,党威,等.中国河南省小麦纹枯病菌对井冈霉素及甲基立枯磷的敏感性[J].农药学学报,2017,19(1):27. XU J Q,PING Z L,DANG W,et al.Sensitivity of Rhizoctonia cerealis to jinggangmycin and tolclofos-methyl in Henan Province in China [J].Chinese Journal of Pesticide Science,2017,19(1):27.
[16]徐建强,杨岚,平忠良,等.中国河南省小麦纹枯病菌对咯菌腈的敏感性[J].农药学学报,2017,19(5):567. XU J Q,YANG L,PING Z L,et al.Sensitivity of Rhizoctonia cerealis to fludioxonil in Henan Province in China [J].Chinese Journal of Pesticide Science,2017,19(5):567.
[17]SUN H Y,LU C Q,LI W,et al.Homozygous and heterozygous point mutations in succinate dehydrogenase subunits b,c and d of Rhizoctonia cerealis conferring resistance to thifluzamide [J].Pest Management Science,2017,73(5):900.
[2]HAMADA M S,YIN Y N,CHEN H G,et al.The escalating threat of Rhizoctonia cerealis ,the causal agent of sharp eyespot in wheat [J].Pest Management Science,2011,67(11):1411.
[3]蒋彦婕,吴纪中,蔡士宾,等.小麦抗纹枯病种质资源的鉴定与评价[J].麦类作物学报,2013,33(3):590. JIANG M J,WU J Z,CAI S B,et al.Screening of resistance to sharp eyespot in wheat germplasm [J].Journal of Triticeae Crops,2013,33(3):590.
[4]徐建强,伍士郎,赵建江,等.河南省小麦纹枯病菌对三唑酮的敏感性[J].植物病理学报,2017,47(1):138. XU J Q,WU S L,ZHAO J J,et al.Sensitivity of Rhizoctonia cerealis to triadimefon in Henan Province [J].Acta Phytopathologica Sinica,2017,47(1):138.
[5]徐建强,刁兴旺,李恒,等.中国河南省小麦纹枯病菌对苯醚甲环唑及戊唑醇的敏感性[J].农药学学报,2016,18(5):583. XU J Q,DIAO X W,LI H,et al.Sensitivity to difenoconazole and tebuconazole of Rhizoctonia cerealis in Henan Province in China [J].Chinese Journal of Pesticide Science,2016,18(5):583.
[6]仇是胜,柏亚罗.琥珀酸脱氢酶抑制剂类杀菌剂的研发进展(Ⅰ)[J].现代农药,2014,13(6):2. QIU S S,BAI Y L.Progress on research and development of succinate dehydrogenase inhibitor fungicides(Ⅰ) [J].Modern Agrochemicals,2014,13(6):2.
[7]宋立妹,张军民.小麦纹枯病菌对氟酰胺的敏感性[J].麦类作物学报,2013,33(6):1288. SONG L M, ZHANG J M.Sensitivity of Rhizoctonia cerealis to flutolanil [J].Journal of Triticeae Crops,2013,33(6):1288.
[8]仇是胜,柏亚罗.琥珀酸脱氢酶抑制剂类杀菌剂的研发进展(Ⅱ)[J].现代农药,2015,14(1):2. QIU S S,BAI Y L.Progress on research and development of succinate dehydrogenase inhibitor fungicides(Ⅱ) [J].Modern Agrochemicals,2015,14(1):2.
[9]魏松红,王海宁,胡吉祥,等.吉林地区水稻纹枯病菌对噻呋酰胺药物的敏感基线探讨[J].沈阳农业大学学报,2016,47(3):282. WEI S H,WANG H N,HU J X,et al.Sensitive baseline of Rhizoctonia solani to thifluzamide in Jilin Province [J].Journal of Shenyang Agricultural University,2016,47(3):282.
[10]中华人民共和国农业部农药检定所.农药登记数据:作物名称:水稻,小麦;有效成分Ⅰ—噻呋酰胺[EB/OL].中国农药信息网http://www.chinapesticide.gov.cn/hysj/index.jhtml,2017-7-7/2017-7-7. Pesticide Examimnnation Institute of the Ministry of Agriculture of the People's Repulic of China.Pesticide Registration Date,Crop:Rice or Wheat,Active Ingredient,Thifluzamide [EB/OL].China pesticide information network,http://www.chinapesticide.gov.cn/hysj/index.jhtml,2017-7-7/2017-7-7.
[11]齐永志,李海燕,苏媛,等.小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状[J].农药学学报,2014,16(3):274. QI Y Z,LI H Y,SU Y,et al.Sensitivity to trifluzamide and main biological characteristics of resistant mutants of Rhizoctonia cerealis [J].Chinese Journal of Pesticide Science,2014,16(3):274.
[12]SUN H Y,WANG C F,LI W,et al.Characterization of Rhizoctonia cerealis sensitivity to thifluzamide in China [J].Crop Protection,2015,69(3):67.
[13]ZHANG Y,LU J L,WANG J X,et al.Baseline sensitivity and resistance risk assessmemt of Rhizoctonia cerealis to thifluzamide,a succinate dehydrogenase inhibitor [J].Pesticide Biochemistry and Physiology,2015,124(10):99.
[14]任学祥,叶正和,丁克坚,等.噻呋酰胺种衣剂防治小麦纹枯病效果及安全性研究[J].麦类作物学报,2015,35(11):1590-1591. REN X X,YE Z H,DING K J,et al.Effect of seed coating treatments with thifluzamide on wheat sharp eyespot and safety evaluation [J].Journal of Triticeae Crops,2015,35(11):1590.
[15]徐建强,平忠良,党威,等.中国河南省小麦纹枯病菌对井冈霉素及甲基立枯磷的敏感性[J].农药学学报,2017,19(1):27. XU J Q,PING Z L,DANG W,et al.Sensitivity of Rhizoctonia cerealis to jinggangmycin and tolclofos-methyl in Henan Province in China [J].Chinese Journal of Pesticide Science,2017,19(1):27.
[16]徐建强,杨岚,平忠良,等.中国河南省小麦纹枯病菌对咯菌腈的敏感性[J].农药学学报,2017,19(5):567. XU J Q,YANG L,PING Z L,et al.Sensitivity of Rhizoctonia cerealis to fludioxonil in Henan Province in China [J].Chinese Journal of Pesticide Science,2017,19(5):567.
[17]SUN H Y,LU C Q,LI W,et al.Homozygous and heterozygous point mutations in succinate dehydrogenase subunits b,c and d of Rhizoctonia cerealis conferring resistance to thifluzamide [J].Pest Management Science,2017,73(5):900.
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