日粮添加姜黄素对IUGR鼠肌肉抗氧化功能及糖代谢的影响
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摘要
本试验研究日粮添加姜黄素对宫内发育迟缓(intrauterine growth retardation,IUGR)鼠肌肉抗氧化功能和糖代谢的调节作用。试验采用2×2因子设计,采用日粮限饲的方法构建IUGR鼠模型。21日龄断奶后,分别选取12只正常和12只IUGR小鼠随机均分为正常组(NBW)、姜黄素组(NC)和IUGR组、IUGR姜黄素组(IC)。NBW和IUGR组饲喂基础日粮,NC和IC组饲喂基础日粮+400 mg/kg姜黄素。试验饲喂至12周龄,取骨骼肌检测抗氧化和糖代谢酶活力以及相关基因的表达。结果表明:与NBW组相比,IUGR组大鼠肌肉糖酵解潜值(glycolytic potential,GP)和乳酸含量显著升高(p<0.05),总还原型谷胱甘肽(total glutathione,T-GSH)含量、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和过氧化氢酶(catalase,CAT)活力显著降低(p<0.05);日粮添加姜黄素可显著降低IUGR大鼠肌肉乳酸脱氢酶活性(p<0.05),显著升高(p<0.05)总超氧化物歧化酶活力、CAT活力、糖原含量、琥珀酸脱氢酶活性以及GP。与NBW组相比,IUGR组大鼠肌肉核因子NF-E2相关因子2(nuclear factor erythroid-2 related factor 2,Nrf2)、超氧化物歧化酶1(superoxide dismutase,SOD1)mRNA表达水平均显著降低(p<0.05)。日粮添加姜黄素可显著提高IUGR组大鼠肌肉Nrf2、SOD1 mRNA的表达水平(p<0.05),显著降低腺苷一磷酸依赖的蛋白激酶(adenosine monophosphate-activated protein kinase α1,AMPKα1)和糖原合成酶(glycogen synthase,GS)mRNA的表达水平(p<0.05)。结论:IUGR鼠肌肉抗氧化能力降低,糖原储存能力下降。姜黄素可能通过调节Nrf2、AMPKα1及其他相关基因的表达,来提高IUGR大鼠肌肉抗氧化能力和抑制糖原分解的作用。
The present study was designed to evaluate the effects of dietary supplementation of curcumin on anti-oxidation and glucose metabolism in the muscle of IUGR(intrauterine growth retardation)rats. Twelve normal and twelve IUGR newborn rats were randomly assigned to the following four experimental groups:NBW group(normal rats,fed with basic diets),NC group(normal rats,fed with basic diets+400 mg/kg curcumin),IC group(IUGR rats,fed with basic diets)and IC group(IUGR rats,fed with basic diets+400 mg/kg curcumin). Rats were fed to 12 weeks of age,including a one-week adaptive phase for weaning. Results:Compared with NBW group,IUGR rats had a higher content of LC and GP(glycolytic potential)in the muscle(p<0.05)but had a lower content of T-GSH. Furthermore,the activities of GSH-PX and CAT(catalase)significantly decreased in muscle of the IUGR rats(p<0.05)as compared with those of NBW group. Curcumin significantly reduced the activities of LDH(p<0.05),whereas increased the activities of T-SOD、CAT and SDH in the muscle of IUGR rats(p<0.05),the content of GLY and GP also increased. Compared with NBW group,the mRNA expression of Nrf2(nuclear factor erythroid-2 related factor 2)and SOD1(superoxide dismutase 1)in the muscle of IUGR rats were significantly down-regulated(p<0.05).However,curcumin up-regulated the mRNA expressions of Nrf2 and SOD1(p<0.05),whereas down-regulated the mRNA expressions of AMPKα1(Adenosine monophosphate-activated protein kinase α1)and GS(glycogen synthase). Conclusions:The anti-oxidant capacity and the glycogen storage capacity of IUGR rats decreased. Curcumin could increase the antioxidant capacity of muscle and inhibit the breakdown of glycogen in IUGR rats by regulating the expression of Nrf2,AMPKα1 and other related genes.
The present study was designed to evaluate the effects of dietary supplementation of curcumin on anti-oxidation and glucose metabolism in the muscle of IUGR(intrauterine growth retardation)rats. Twelve normal and twelve IUGR newborn rats were randomly assigned to the following four experimental groups:NBW group(normal rats,fed with basic diets),NC group(normal rats,fed with basic diets+400 mg/kg curcumin),IC group(IUGR rats,fed with basic diets)and IC group(IUGR rats,fed with basic diets+400 mg/kg curcumin). Rats were fed to 12 weeks of age,including a one-week adaptive phase for weaning. Results:Compared with NBW group,IUGR rats had a higher content of LC and GP(glycolytic potential)in the muscle(p<0.05)but had a lower content of T-GSH. Furthermore,the activities of GSH-PX and CAT(catalase)significantly decreased in muscle of the IUGR rats(p<0.05)as compared with those of NBW group. Curcumin significantly reduced the activities of LDH(p<0.05),whereas increased the activities of T-SOD、CAT and SDH in the muscle of IUGR rats(p<0.05),the content of GLY and GP also increased. Compared with NBW group,the mRNA expression of Nrf2(nuclear factor erythroid-2 related factor 2)and SOD1(superoxide dismutase 1)in the muscle of IUGR rats were significantly down-regulated(p<0.05).However,curcumin up-regulated the mRNA expressions of Nrf2 and SOD1(p<0.05),whereas down-regulated the mRNA expressions of AMPKα1(Adenosine monophosphate-activated protein kinase α1)and GS(glycogen synthase). Conclusions:The anti-oxidant capacity and the glycogen storage capacity of IUGR rats decreased. Curcumin could increase the antioxidant capacity of muscle and inhibit the breakdown of glycogen in IUGR rats by regulating the expression of Nrf2,AMPKα1 and other related genes.
引文
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[30]黄小平,邓常清,邱咏园,等.黄芪甲苷和三七的三种有效成分配伍对小鼠脑缺血/再灌注后氧化应激和Nrf2/HO1途径的影响[J].中国药理学通报,2013,29(11):1596-1601.
[31]Yin J,Ren W,Liu G,et al.Birth oxidative stress and the development of an antioxidant system in newborn piglets[J].FreeRadical Research,2013,47(12):1027-1035.
[32]Hao Z,Yue L,Tian W.Antioxidant capacity and concentration of redox-active trace mineral in fully weaned intra-uterine growth retardation piglets[J].Journal of Animal Science and Biotechnology,2016,6(2):201-207.
[33]何惠君,王国玉,高渊,等.姜黄素活化肝细胞Nrf2抗氧化系统效应及缓解胰岛素抵抗作用机制研究[J].热带医学杂志,2012(7):785-798.
[34]李昌哲,李军,张爱华,等.姜黄素对饮水砷暴露大鼠肝脏氧化损伤的干预作用[J].中华地方病学杂志,2015,34(6):406-410.
[35]Corton J M,Gillespie J G,Hardie D G.Role of the AMP-activated protein kinase in the cellular stress response[J].Current Biology Cb,1994,4(4):315.
[36]李泽,马霞,靳烨.不同年龄和部位羊肉中AMPK活性与糖酵解的差异[J].食品与发酵工业,2010(1):184-186.
[2]Asea A,Kraeft S K,Kurt E A,et al.Hsp70 stimulates cytokine production through a CD14-dependant pathway,demonstrating its dual role as a chaperone and cytokine[J].Nature Medicine,2000,6(4):435-442.
[3]Bobak M.Outdoor A P,low birth weight,and prematurity[J].Environmental Health Perspectivest,2000,108(2):173-176.
[4]Liu J B,He J.Effects of birth weight and postnatal high-fat diet on growth performance,carcass and meat quality in pigs[J].Japs Journal of Animal & Plant Sciences,2014,24(6):1606-1612.
[5]Nissen P M,Oksbjerg N.Birth weight and postnatal dietary protein level affect performance,muscle metabolism and meat quality in pigs[J].Animal,2011,5(9):1382-1389.
[6]Rosenberg A.The IUGR newborn[J].Seminars in Perinatology,2008,32(3):219-224.
[7]Fernandez X,Santé V,Baeza E,et al.Effects of the rate of muscle post mortem pH fall on the technological quality of turkey meat[J].British Poultry Science,2002,43(2):245-252.
[8]Li W,Suwanwela N C,Patumraj S.Curcumin by down regulating NF-κB and elevating Nrf2,reduces brain edema and neurological dysfunction after cerebral I/R[J].Microvascular Research,2015,12(8):117-127.
[9]狄建彬,顾振纶,赵笑东,等.姜黄素的抗氧化和抗炎作用研究进展[J].中草药,2010,41(5):854-857.
[10]祝国强,张伟涛,陈涛,等.姜黄素添加剂对育肥猪胴体GP、肉品质及血液生化指标的影响[J].饲料工业,2013(16):9-12.
[11]Desai M,Gayle D,Babu J,et al.Programmed obesity in intrauterine growth-restricted newborns:Modulation by newborn nutrition[J].American Journal of Physiology Regulatory Integrative & Comparative Physiology,2005,288(1):R91.
[12]Taimur S,Nida S,Sanna F,et al.Intrauterine growth retardation-small events,big consequences[J].Italian Journal of Pediatrics,2011,37(1):41.
[13]Jintian H,Yu N,Fei W,et al.Dietary curcumin supplementation attenuates inflammation,hepatic injury and oxidative damage in a rat model of intra-uterine growth retardation[J].British Journal of Nutrition,2018:1-12.
[14]Reeves P G,Nielsen F H,Jr F G.AIN-93 purified diets for laboratory rodents:Final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J].Journal of Nutrition,1993,123(11):1939.
[15]Sibley C P,Turner M A,Cetin I,et al.Placental phenotypes of intrauterine growth[J].Pediatric Research,2005,58(5):827-832.
[16]Alomar M A,Beedham C,Alsarra I A.Pathological roles of reactive oxygen species and their defence mechanism[J].Saudi Pharmaceutical Journal,2003,12(1):1-15.
[17]李博,李伟,张昊,等.宫内发育迟缓对哺乳仔猪生长性能和肝脏氧化及抗氧化指标的影响[J].动物营养学报,2014,26(9):2822-2827.
[18]Olsvik P A,Kristensen T,Waagb R,et al.mRNA expression of antioxidant enzymes(SOD,CAT and GSH-Px)and lipid peroxidative stress in liver of Atlantic salmon(Salmo salar)exposed to hyperoxic water during smoltification[J].Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology,2005,141(3):0-323.
[19]何进田,董丽,白凯文,等.三丁酸甘油酯对宫内发育迟缓哺乳仔猪肝脏抗氧化和线粒体功能的影响[J].食品科学,2016,37(3):191-196.
[20]苏伟鹏,徐稳,张昊,等.日粮亮氨酸水平与宫内发育迟缓对断奶仔猪肝脏抗氧化功能的影响[J].食品工业科技,2016,37(19):345-351.
[21]张莉莉,王远孝,孔一力,等.精氨酸对子宫内发育迟缓仔猪抗氧化功能和精氨酸代谢的影响[J].南京农业大学学报,2017(6):1111-1118.
[22]王海华,蒋明义,汪琼,等.几种抗氧化剂对羟自由基的体外清除作用[J].矿业工程研究,2002,24(2):87-90.
[23]Rajeswari A.Curcumin protects mouse brain from oxidative stress caused by 1-methy-1-4-phenyl-1,2,3,6-tetrahydropyridine[J].European Review for Medical & Pharmacological Sciences,2006,10(4):157-161.
[24]Wadley G D,Siebel A L,Cooney G J,et al.Uteroplacental insufficiency and reducing litter size alters skeletal muscle mitochondrial biogenesis in a sex-specific manner in the adult rat[J].American Journal of Physiology Endocrinology & Metabolism,2008,294(1):861-869.
[25]李博,李伟,张昊,等.日粮高胆碱水平对宫内发育迟缓猪背最长肌糖酵解的影响[J].南京农业大学学报,2015,38(2):324-329.
[26]Immonen K,Ruusunen M,Hissa K,et al.Bovine muscle glycogen concentration in relation to finishing diet,slaughter and ultimate pH[J].Meat Science,2000,55(1):25-31.
[27]Thorn S R,Sekar S M,Lavezzi J R,et al.A physiological increase in insulin suppresses gluconeogenic gene activation in fetal sheep with sustained hypoglycemia[J].Ajp Regulatory Integrative & Comparative Physiology,2012,303(8):R861-R869.
[28]Huang H C,Zheng B W,Guo Y,et al.Antioxidative and neuroprotective effects of curcumin in an alzheimer’s disease rat model co-treated with intracerebroventricular streptozotocin and subcutaneous D-galactose[J].Journal of Alzheimers Disease Jad,2016,52(3):899.
[29]徐明生,陈锦屏,上官新晨.鱼精蛋白对黑曲霉细胞内的琥珀酸脱氢酶和苹果酸脱氢酶的影响[J].食品科学,2005,26(4):48-51.
[30]黄小平,邓常清,邱咏园,等.黄芪甲苷和三七的三种有效成分配伍对小鼠脑缺血/再灌注后氧化应激和Nrf2/HO1途径的影响[J].中国药理学通报,2013,29(11):1596-1601.
[31]Yin J,Ren W,Liu G,et al.Birth oxidative stress and the development of an antioxidant system in newborn piglets[J].FreeRadical Research,2013,47(12):1027-1035.
[32]Hao Z,Yue L,Tian W.Antioxidant capacity and concentration of redox-active trace mineral in fully weaned intra-uterine growth retardation piglets[J].Journal of Animal Science and Biotechnology,2016,6(2):201-207.
[33]何惠君,王国玉,高渊,等.姜黄素活化肝细胞Nrf2抗氧化系统效应及缓解胰岛素抵抗作用机制研究[J].热带医学杂志,2012(7):785-798.
[34]李昌哲,李军,张爱华,等.姜黄素对饮水砷暴露大鼠肝脏氧化损伤的干预作用[J].中华地方病学杂志,2015,34(6):406-410.
[35]Corton J M,Gillespie J G,Hardie D G.Role of the AMP-activated protein kinase in the cellular stress response[J].Current Biology Cb,1994,4(4):315.
[36]李泽,马霞,靳烨.不同年龄和部位羊肉中AMPK活性与糖酵解的差异[J].食品与发酵工业,2010(1):184-186.