镇肝熄风汤对自发性高血压大鼠JAK2mRNA和蛋白表达的影响
|
摘要
目的:研究不同剂量的镇肝熄风汤对自发性高血压大鼠(SHR)腹主动脉血管组织JAK2蛋白和JAK2 mRNA表达的影响。方法:方法:将24周龄60只SHR大鼠随机分为:模型组、镇肝熄风汤中药低剂量组、中剂量组、高剂量组和复方罗布麻组,每组12只,正常对照组为:雄性12只,同源魏-凯二氏大鼠(WKY)。连续灌胃给药45天后,Western Blot测定腹主动脉血管组织中JAK2蛋白表达,实时聚合酶链反应(RT-PCR)方法测定大鼠JAK2 mRNA基因表达。结果:与模型组比较,镇肝熄风汤中药中剂量和高剂量组JAK2蛋白表达和JAK2 mRNA表达明显降低(F=74. 745,sig=0. 000,P <0. 05)。结论:镇肝熄风汤可以抑制腹主动脉血管组织中JAK2蛋白和JAK2 mRNA表达,减少血管细胞增生,起到降血压的作用。
Objective: To study the effect of different doses of Zhengan Xifeng Decoction on JAK2 mRNA and protein expressions in the abdominal aortic vascular tissue of spontaneously hypertensive rats( SHR). Methods: A total of 60 male SHR which were 24 weeks old,were randomly divided into the model,low,medium and high dose of Zhengan Xifeng Decoction and the compound apocynum groups,12 in each group. Twelve homologous male rats( WKY) were served as the normal control group. After gavage for 45 days,Western blotting and RT-PCR were used to detect the JAK2 protein and JAK2 expressions in the abdominal aortic vascular tissue. Results: Compared with the model group,the JAK2 protein and JAK2 mRNA expressions in the medium and high dose groups were significantly reduced( F = 74. 745,sig = 0. 000,P < 0. 05). Conclusion: Zhengan Xifeng Decoction can inhibit the expressions of JAK2 protein and JAK2 mRNA in the abdominal aortic vascular tissue,playing a role in decreasing vascular cell proliferation and lowing blood pressure.
Objective: To study the effect of different doses of Zhengan Xifeng Decoction on JAK2 mRNA and protein expressions in the abdominal aortic vascular tissue of spontaneously hypertensive rats( SHR). Methods: A total of 60 male SHR which were 24 weeks old,were randomly divided into the model,low,medium and high dose of Zhengan Xifeng Decoction and the compound apocynum groups,12 in each group. Twelve homologous male rats( WKY) were served as the normal control group. After gavage for 45 days,Western blotting and RT-PCR were used to detect the JAK2 protein and JAK2 expressions in the abdominal aortic vascular tissue. Results: Compared with the model group,the JAK2 protein and JAK2 mRNA expressions in the medium and high dose groups were significantly reduced( F = 74. 745,sig = 0. 000,P < 0. 05). Conclusion: Zhengan Xifeng Decoction can inhibit the expressions of JAK2 protein and JAK2 mRNA in the abdominal aortic vascular tissue,playing a role in decreasing vascular cell proliferation and lowing blood pressure.
引文
[1]Cai B1,Cai JP,Luo YL,et al. The Specific Roles of JAK/STAT Signaling Pathway in Sepsis[J]. Inflammation,2015,38(4):1599-1608.
[2]Chai HT,Yip HK,Sun CK,et al. AG490 suppresses EPO-mediated activation of JAK2-STAT but enhances blood flow recovery in rats with critical limb ischemia[J]. J Inflamm,2016,4(13):18.
[3]Mattar MM,Morad MA,El Husseiny NM,et al. Correlation between JAK2 allele burden and pulmonary arterial hypertension and hematological parameters in Philadelphia negative JAK2 positive myeloproliferative neoplasms[J]. Ann Hematol,2016,95(10):1611-1616.
[4]Xia Y,Xia H,Chen D,et al. Mechanisms of autophagy and apoptosis mediated by JAK2 signaling pathway after spinal cord injury of rats[J]. Exp Ther Med,2017,14(2):1589-1593.
[5]Popat U,Frost A,Liu E,et al. High levels of circulating CD34 cells,dacrocytes,clonal hematopoiesis,and JAK2mutation differentiate myelofibrosis with myeloid metaplasia from secondary myelofibrosis associated with pulmonary hypertension[J]. Blood,2006,107(9):3486-3488.
[6]Dai F,Gao L,Zhao Y,et al. Farrerol inhibited angiogenesis through Akt/m TOR,Erk and Jak2/Stat3 signal pathway[J]. Phytomedicine,2016,23(7):686-693.
[7]Kirabo A1,Kearns PN,Jarajapu YP,et al. Vascular smooth muscle Jak2 mediates angiotensin II-induced hypertension via increased levels of reactive oxygen species[J]. Cardiovasc Res,2011,91(1):171-179.
[8]Beckles DL,Mascareno E,Siddiqui MA. Inhibition of Jak2 phosphorylation attenuates pressure overload cardiac hypertrophy[J]. Vascul Pharmacol,2006,45(6):350-357.
[9]Gottardello Zecchin H,De Souza CT,Oliveira Prada P,et al. Effect of obesity on insulin signaling through JAK2 in rat aorta[J]. Vascul Pharmacol,2005,43(5):346-352.
[10]Valdembri D,Serini G,Vacca A,et al. In vivo activation of JAK2/STAT-3 pathway during angiogenesis induced by GM-CSF[J].FASEB J,2002,16(2):225-227.
[11]谢鑫,陈士玉,张林,等.镇肝熄风汤对自发性高血压大鼠Bcl-2mRNA和蛋白表达的影响[J].中华中医药学刊,2014,32(4):853-855.
[12]Xu XP,He HL,Hu SL,et al. Ang II-JAK2 increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42pathways in vitro[J]. Stem Cell Res Ther,2017,8(1):164.
[13]Imada K,Leonard WJ. The Jak-STAT pathway[J]. Mol Immunol,2000,37(1-2):1-11.
[14]Wu L,Chen G,Liu W,et al. Intramuscular injection of exogenous leptin induces adiposity,glucose intolerance and fatty liver by repressing the JAK2-STAT3/PI3K pathway in a rat model[J]. Gen Comp Endocrinol,2017,252:88-96.
[15]Xu X,Han K,Zhu J,et al. An inhibitor of cholesterol absorption displays anti-myeloma activity by targeting the JAK2-STAT3 signaling pathway[J]. Oncotarget,2016,7(46):75539-75550.
[16]Ge D,Gooljar SB,Kyriakou T,et al. Association of common JAK2 variants with body fat,insulin sensitivity and lipid profile[J]. Obesity(Silver Spring),2008,16(2):492-496.
[17]Tang C,Vaughan AM,Oram JF. Janus kinase 2 modulates the apolipoprotein interactions with ABCA1 required for removing cellular cholesterol[J]. J Biol Chem,2004,279(9):7622-7628.
[18]Heim MH. The Jak-STAT pathway:cytokine signalling from the receptor to the nucleus[J]. J Recept Signal Transduct Res,1999,19(1-4):75-120.
[19]孟宪卿,姜月华,吴赛,等.刺蒺藜通过瘦素介导的JAK2/STAT3通路对肥胖性高血压大鼠肾脏的影响[J].中草药,48(3):539-545.
[20]姜坤,高璇,宋静.镇肝熄风汤对高血压病人血液流变学的影响[J].中国血液流变学杂志,2004,14(2):180-239.
[21]谢鑫,杨芳,张林,等.镇肝熄风汤对自发性高血压大鼠PCNA mRNA和蛋白表达的影响[J].天然产物研究与开发,2014,26(4):610-612.
[2]Chai HT,Yip HK,Sun CK,et al. AG490 suppresses EPO-mediated activation of JAK2-STAT but enhances blood flow recovery in rats with critical limb ischemia[J]. J Inflamm,2016,4(13):18.
[3]Mattar MM,Morad MA,El Husseiny NM,et al. Correlation between JAK2 allele burden and pulmonary arterial hypertension and hematological parameters in Philadelphia negative JAK2 positive myeloproliferative neoplasms[J]. Ann Hematol,2016,95(10):1611-1616.
[4]Xia Y,Xia H,Chen D,et al. Mechanisms of autophagy and apoptosis mediated by JAK2 signaling pathway after spinal cord injury of rats[J]. Exp Ther Med,2017,14(2):1589-1593.
[5]Popat U,Frost A,Liu E,et al. High levels of circulating CD34 cells,dacrocytes,clonal hematopoiesis,and JAK2mutation differentiate myelofibrosis with myeloid metaplasia from secondary myelofibrosis associated with pulmonary hypertension[J]. Blood,2006,107(9):3486-3488.
[6]Dai F,Gao L,Zhao Y,et al. Farrerol inhibited angiogenesis through Akt/m TOR,Erk and Jak2/Stat3 signal pathway[J]. Phytomedicine,2016,23(7):686-693.
[7]Kirabo A1,Kearns PN,Jarajapu YP,et al. Vascular smooth muscle Jak2 mediates angiotensin II-induced hypertension via increased levels of reactive oxygen species[J]. Cardiovasc Res,2011,91(1):171-179.
[8]Beckles DL,Mascareno E,Siddiqui MA. Inhibition of Jak2 phosphorylation attenuates pressure overload cardiac hypertrophy[J]. Vascul Pharmacol,2006,45(6):350-357.
[9]Gottardello Zecchin H,De Souza CT,Oliveira Prada P,et al. Effect of obesity on insulin signaling through JAK2 in rat aorta[J]. Vascul Pharmacol,2005,43(5):346-352.
[10]Valdembri D,Serini G,Vacca A,et al. In vivo activation of JAK2/STAT-3 pathway during angiogenesis induced by GM-CSF[J].FASEB J,2002,16(2):225-227.
[11]谢鑫,陈士玉,张林,等.镇肝熄风汤对自发性高血压大鼠Bcl-2mRNA和蛋白表达的影响[J].中华中医药学刊,2014,32(4):853-855.
[12]Xu XP,He HL,Hu SL,et al. Ang II-JAK2 increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42pathways in vitro[J]. Stem Cell Res Ther,2017,8(1):164.
[13]Imada K,Leonard WJ. The Jak-STAT pathway[J]. Mol Immunol,2000,37(1-2):1-11.
[14]Wu L,Chen G,Liu W,et al. Intramuscular injection of exogenous leptin induces adiposity,glucose intolerance and fatty liver by repressing the JAK2-STAT3/PI3K pathway in a rat model[J]. Gen Comp Endocrinol,2017,252:88-96.
[15]Xu X,Han K,Zhu J,et al. An inhibitor of cholesterol absorption displays anti-myeloma activity by targeting the JAK2-STAT3 signaling pathway[J]. Oncotarget,2016,7(46):75539-75550.
[16]Ge D,Gooljar SB,Kyriakou T,et al. Association of common JAK2 variants with body fat,insulin sensitivity and lipid profile[J]. Obesity(Silver Spring),2008,16(2):492-496.
[17]Tang C,Vaughan AM,Oram JF. Janus kinase 2 modulates the apolipoprotein interactions with ABCA1 required for removing cellular cholesterol[J]. J Biol Chem,2004,279(9):7622-7628.
[18]Heim MH. The Jak-STAT pathway:cytokine signalling from the receptor to the nucleus[J]. J Recept Signal Transduct Res,1999,19(1-4):75-120.
[19]孟宪卿,姜月华,吴赛,等.刺蒺藜通过瘦素介导的JAK2/STAT3通路对肥胖性高血压大鼠肾脏的影响[J].中草药,48(3):539-545.
[20]姜坤,高璇,宋静.镇肝熄风汤对高血压病人血液流变学的影响[J].中国血液流变学杂志,2004,14(2):180-239.
[21]谢鑫,杨芳,张林,等.镇肝熄风汤对自发性高血压大鼠PCNA mRNA和蛋白表达的影响[J].天然产物研究与开发,2014,26(4):610-612.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |