SDF-1α及其受体CXCR4在类风湿关节炎患者外周血中的表达
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摘要
目的探讨基质细胞衍生因子SDF-1α及其受体CXCR4在类风湿关节炎(RA)发生、发展中的作用及意义。方法用流式细胞术对50例RA患者和25例健康志愿者外周血CD3+CD4+T细胞、CD3+CD8+T细胞表面CXCR4的表达水平进行检测,用ELISA法检测SDF-1α、IL-6在上述两组外周血中的表达情况。结果 RA患者组外周血中CD3+CD4+T细胞和CD3+CD8+T细胞表面CXCR4表达水平和SDF-1α、IL-6的表达均明显高于健康对照组(P<0.05)。直线相关性分析显示,外周血CXCR4及SDF-1α表达水平与IL-6均存在正相关性(P<0.05)。结论 SDF-1α/CXCR4轴及IL-6在RA患者的发病过程中可能起到了重要作用,并且二者有协同作用。
Objective To study the expression of SDF-1αand CXCR4 in the peripheral blood patients with rheumatoid arthritis(RA).Methods 50 RA patients were recruited and 25 healthy volunteers were enrolled as normal controls.The expressions of CXCR4 on CD3~+CD4~+T cells and CD3~+CD8~+T cells were detected by flow cytometry in 50 patients with RA and 25 healthy subjects.The level of SDF-1αand IL-6 were detected by ELISA.Results The expression of CXCR4 on CD3~+CD4~+T cells and CD3~+CD8~+T cells and the levels of SDF-1αand IL-6 in peripheral blood of patients with rheumatoid arthritis were significantly higher than those of healthy controls(P<0.05).Linear correlation analysis showed that there was a positive correlation between CXCR4 and SDF-1αexpression in peripheral blood and IL-6(P<0.05).Conclusion SDF-1α/CXCR4 axis and IL-6 maybe play an important role in the pathogenesis of rheumatoid arthritis.
Objective To study the expression of SDF-1αand CXCR4 in the peripheral blood patients with rheumatoid arthritis(RA).Methods 50 RA patients were recruited and 25 healthy volunteers were enrolled as normal controls.The expressions of CXCR4 on CD3~+CD4~+T cells and CD3~+CD8~+T cells were detected by flow cytometry in 50 patients with RA and 25 healthy subjects.The level of SDF-1αand IL-6 were detected by ELISA.Results The expression of CXCR4 on CD3~+CD4~+T cells and CD3~+CD8~+T cells and the levels of SDF-1αand IL-6 in peripheral blood of patients with rheumatoid arthritis were significantly higher than those of healthy controls(P<0.05).Linear correlation analysis showed that there was a positive correlation between CXCR4 and SDF-1αexpression in peripheral blood and IL-6(P<0.05).Conclusion SDF-1α/CXCR4 axis and IL-6 maybe play an important role in the pathogenesis of rheumatoid arthritis.
引文
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[22]祖蓓蓓,饶咏梅,李美荣,等.狼疮肾炎患者外周血中趋化因子受体CXCR4及其配体SDF-1α的表达研究[J].临床和实验医学杂志,2013,12(23):1884-1887.
[23]Kanbe K,Chiba J,Inoue Y,et al.SDF-1and CXCR4in synovium are associated with disease activity and bone and joint destruction in patients with rheumatoid arthritis treated with golimumab[J].Mod Rheumatol,2016,26(1):46-50.
[24]陈言,王晓燕,姜林宏等.SDF-1在大鼠颞下颌关节骨关节炎相关性的研究[J].口腔颌面修复外科杂志,2017,18(1):37-42.
[25]李龙滕,李彦林,王坤,等.TN14003体外阻断SDF-1/CXCR4信号通路对骨关节炎患者软骨组织分泌基质金属蛋白酶3、9、13水平的影响[J].中国运动医学杂志,2017,36(1):44-47.
[26]饶咏梅,祖蓓蓓,刘琳,等.趋化因子基质细胞衍生因子-1α及其受体CXCR4在系统性红斑狼疮血液系统损害中的表达研究[J].中华临床医师杂志,2013,7(9):3820-3823.
[27]Ruderman EM.Rheumatoid arthritis:IL-6inhibition in RA-dejavu all over again[J].Nat Rev Rheumatol,2015,11(6):321-322.
[2]Sch9nfeld C,Pap T,Neumann E,et al.Fibroblasts as pathgenic cells in rheumatoid arthritis[J].Z Rheumatol,2015,74(1):33-38.
[3]Fei Yu,Ying Xie,Yan Wang,et al.Chloroquine containing HPMA copolymers as polymeric inhibitors of cancer cell migration mediated by the CXCR4/SDF-1chemokine axis[J].ACS Macro Letters,2016,5(3):342.
[4]Kanbe K,Takemura T,Takeuchi K,et al.Synovectomy reduces stromal-cell-derived factor-1(SDF-1)which is involved in the destruction of cartilage in osteoarthritis and rheumatoid arthritis[J].J Bone Joint Surg,2004,86(6):296-300.
[5]Kanbe K,Takagishik K,Chen Q.Stimulation of matrix metalloprotease-3release from human chondrocytes by the interaction of stromal cell derived factor-1and CXC chemokine receptor4[J].Arthritis Rheum,2002,46:130-137.
[6]Brown MP,Dymock DC,Merritt KA.Stromal-cell-derived factor-1(SDF-1)validation using equine serum,plasma,and synovial fluid[J].Osteoarthritis and Cartilage,2014,S71.
[7]Wei Li,Sun X,Kanbe K,et al.Chondrocyte death induced by pathological concentration of chemokine stromal-cell-derived factor-1[J].J Rheumatol,2006,33(9):1818-1826.
[8]冯昭,涂亚庭,曾凡杞.趋化因子SDF-1及其受体CXCR4在皮肌炎皮损中的表达[J].中国皮肤性病学杂志,2005,19(11):654-657.
[9]古洁若,黄烽,Jatinderpal Kalsi,等.趋化因子受体CXCR4及其配体SDF-1与强直性脊柱炎发病的相关性研究[J].中国病理生理杂志,2005,21(2):224-229.
[10]祖蓓蓓,刘琳,饶咏梅,等.系统性红斑狼疮患者外周血中趋化因子基质细胞衍生因子-1α及其受体CXC趋化因子受体4的检测及其临床意义[J].中华风湿病学杂志,2013,17(12):828-832.
[11]Kotrych D,Dziedziejko V,Safronow K,et al.CXCL9and CXCL10gene polymorphisms in patients with rheumatoid arthritis[J].Rheumatol Int,2015,35(8):1319-1323.
[12]Antonelli A,Ferrari SM,GiuggioliD,et al.Chemokine(C-X-C motif)ligand(CXC)10in autoimmune disease[J].Autoimmun Rev,2014,13(3):272-280.
[13]Kuan WP,Tam LS,Wong CK,et al.CXCL9and CXCL10as sensitive markers of disease activity in patients with rheumatoid arthritis[J].J Rheumatol,2010,37(2):257-264.
[14]Rosengren S,Kalunian KC,Kavanaugh A,et al.CXCL13as a marker for outcome of rheumatoid arthritis:comment on the article by Meeuwisse et al.[J].Arthritis Rheum,2011,63(11):3646-3647.
[15]Bugatti S,Manzo A,Benaglio F,et al.Serum levels of CXCL13are associated with ultrasonographic synovitis and predict power Doppler persistence in early rheumatoid arthritis treated with nonbiological disease-modifying anti-rheummatic drugs[J].Arthritis Res Ther,2012,14(1):34.
[16]Grassi F,Cristino S,Toneguzzi S,et al.CXCL12chemokine upregulates bone resorption and MMP-9release by human osteoclasts:CXCL12levels are increased in synovial and bone tissue of rheumatoid arthritis patients[J].J Cell Physiol,2004,199(2):244-251.
[17]Pablos JL,Santiago B,Galindo M,et al.Synoviocyte-derived CXCL12is displayed on endothelium and induces angiogenesis in rheumatoid arthritis[J].J Immunol,2003,170(4):2147-2152.
[18]石连杰,李建红,胡凡磊,等.趋化因子配体19在类风湿关节炎患者血清中的表达及临床意义[J].北京大学学报(医学版),2016,48(4):667-671.
[19]Sellam J,Rouanet S,Hendel-Chavez H,et al.CCL19,a B cell chemokine,is related to the decrease of blood memory of B cells and predicts the clinical response to rituximab in patients with rheumatoid arthritis[J].Arthritis Rheum,2013,65(9):2253-2261.
[20]Chung SH,Seki K,Choi BI,et al.CXC chemokine receptor 4expressed in Tcells plays an important role in the development of collagen-induced arthritis[J].Arthritis Res Ther,2010,12(5):R188.
[21]张逸彪.类风湿关节炎患者血清和关节液中趋化因子的测定及其意义[J].浙江临床医学,2007,9(10):1349.
[22]祖蓓蓓,饶咏梅,李美荣,等.狼疮肾炎患者外周血中趋化因子受体CXCR4及其配体SDF-1α的表达研究[J].临床和实验医学杂志,2013,12(23):1884-1887.
[23]Kanbe K,Chiba J,Inoue Y,et al.SDF-1and CXCR4in synovium are associated with disease activity and bone and joint destruction in patients with rheumatoid arthritis treated with golimumab[J].Mod Rheumatol,2016,26(1):46-50.
[24]陈言,王晓燕,姜林宏等.SDF-1在大鼠颞下颌关节骨关节炎相关性的研究[J].口腔颌面修复外科杂志,2017,18(1):37-42.
[25]李龙滕,李彦林,王坤,等.TN14003体外阻断SDF-1/CXCR4信号通路对骨关节炎患者软骨组织分泌基质金属蛋白酶3、9、13水平的影响[J].中国运动医学杂志,2017,36(1):44-47.
[26]饶咏梅,祖蓓蓓,刘琳,等.趋化因子基质细胞衍生因子-1α及其受体CXCR4在系统性红斑狼疮血液系统损害中的表达研究[J].中华临床医师杂志,2013,7(9):3820-3823.
[27]Ruderman EM.Rheumatoid arthritis:IL-6inhibition in RA-dejavu all over again[J].Nat Rev Rheumatol,2015,11(6):321-322.