中华弧丽金龟甲对十二种常见植物挥发物的触角电位和嗅觉反应
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摘要
为筛选中华弧丽金龟甲Popillia quadriguttata的行为调控剂,利用触角电位仪测定中华弧丽金龟甲雌、雄成虫对12种挥发物的电生理反应,利用Y型嗅觉仪测试其对8种具有电生理活性的挥发物的行为反应。结果表明:寄主植物挥发物1-辛烯-3-醇引起的中华弧丽金龟甲雌成虫触角电位(EAG)相对反应值分别为己醛的3.08倍、反式-2-己烯醛的2.96倍、3-辛酮的2.33倍,显著高于这3种挥发物;5种非寄主植物挥发物引起的雌成虫EAG相对反应值之间不存在显著差异。在浓度为0.1 mol/L时,寄主植物挥发物2-乙基-1-己醇、1-辛烯-3-醇和β-石竹烯均对雌成虫具有显著引诱作用,非寄主挥发物(-)-β-蒎烯对雄成虫具有显著驱避作用;在浓度为0.001 mol/L时,寄主挥发物2-乙基-1-己醇、1-辛烯-3-醇、β-石竹烯和芳樟醇均对雌成虫具有显著引诱作用,非寄主挥发物(R)-(+)-柠檬烯、1,6-二甲基-2,4,6-辛三烯对雄成虫具有显著引诱作用;在浓度为0.00001 mol/L时,寄主挥发物β-石竹烯、芳樟醇均对雌成虫具有显著引诱作用,非寄主挥发物(R)-(+)-柠檬烯、1,6-二甲基-2,4,6-辛三烯对雄成虫具有显著引诱作用。说明一定浓度的β-石竹烯、芳樟醇、2-乙基-1-己醇对中华弧丽金龟甲雌成虫具有显著的引诱能力,可作为引诱剂成分加以开发利用。
In order to screen for the behavioral regulators of four spotted beetle Popillia quadriguttata,the techniques of electroantennogram(EAG) and Y-tube olfactometer were used to test the electrophysiological activities of P. quadriguttata elicited by 12 chemicals and their behavioral responses to eight chemicals with electrophysiological activity. The results showed that the EAG reaction of female caused by host plant volatile 1-octene-3-ol was significantly higher than that caused by hexanal, trans-2-hexenal and 3-octanone. No significant differences in female EAG response were found among the five nonhost plant volatiles. At the concentration of 0.1 mol/L, the host volatiles 2-ethyl-1-hexanol, 1-octene-3-ol, and β-caryophyllene all had significant attractive effects on females, and non-host volatile(-)-β-pinene had significant repellent effect on males. At the concentration of 0.001 mol/L, host volatiles2-ethyl-1-hexanol, 1-octene-3-ol, β-caryophyllene and linalool had significant attractive effects on females. Non-host volatiles(R)-(+)-limonene and 1,6-dimethyl-2,4,6-octanetriene had significant attractive effects on males. At the concentration of 0.00001 mol/L, the host volatiles β-caryophyllene and linalool both had significant attractive effects on females, while non-host volatiles(R)-(+)-limonene, 1,6-dimethyl-2,4,6-octanetriene had significant attractive effects on males. The results indicated that a certain concentration of β-caryophyllene, linalool, and 2-ethyl-1-hexanol had significant attractive ability to females of P. quadriguttata, and could be considered as attractant components.
In order to screen for the behavioral regulators of four spotted beetle Popillia quadriguttata,the techniques of electroantennogram(EAG) and Y-tube olfactometer were used to test the electrophysiological activities of P. quadriguttata elicited by 12 chemicals and their behavioral responses to eight chemicals with electrophysiological activity. The results showed that the EAG reaction of female caused by host plant volatile 1-octene-3-ol was significantly higher than that caused by hexanal, trans-2-hexenal and 3-octanone. No significant differences in female EAG response were found among the five nonhost plant volatiles. At the concentration of 0.1 mol/L, the host volatiles 2-ethyl-1-hexanol, 1-octene-3-ol, and β-caryophyllene all had significant attractive effects on females, and non-host volatile(-)-β-pinene had significant repellent effect on males. At the concentration of 0.001 mol/L, host volatiles2-ethyl-1-hexanol, 1-octene-3-ol, β-caryophyllene and linalool had significant attractive effects on females. Non-host volatiles(R)-(+)-limonene and 1,6-dimethyl-2,4,6-octanetriene had significant attractive effects on males. At the concentration of 0.00001 mol/L, the host volatiles β-caryophyllene and linalool both had significant attractive effects on females, while non-host volatiles(R)-(+)-limonene, 1,6-dimethyl-2,4,6-octanetriene had significant attractive effects on males. The results indicated that a certain concentration of β-caryophyllene, linalool, and 2-ethyl-1-hexanol had significant attractive ability to females of P. quadriguttata, and could be considered as attractant components.
引文
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Beyaert I,W?schke N,Scholz A,Varama M,Reinecke A,Hilker M.2010.Relevance of resource-indicating key volatiles and habitat odour for insect orientation.Animal Behaviour,79(5):1077-1086
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Fu XX,Xu X,Lu AJ,Bi JR,Su X,Shi SS.2014.Relationship between components change of soybean volatile and Leguminivora glycinivorella infestation.Journal of Jilin Agricultural University,36(4):389-394(in Chinese)[付晓霞,徐伟,卢爱军,毕嘉瑞,苏贤,史树森.2014.大豆植株挥发物组分变化与大豆食心虫为害的关系.吉林农业大学学报,36(4):389-394]
Ge YF,Han JY,Zhou YM,Xu YM,Ding Y,Shi M,Guo CK,Wu G.2018.Silencing of mi R156 confers enhanced resistance to brown planthopper in rice.Planta,https://doi.org/10.1007/s00425-018-2942-2946
Gregg PC,Del Socorro AP,Hawes AJ,Binns MR.2016.Developing bisexual attract-and-kill for polyphagous insects:ecological rationale versus pragmatics.Journal of Chemical Ecology,42(7):666-675
Jin F,Ji BZ,Liu SW,Tian L,Gao J.2009.Deposition modes,components and functions of secretions associated with oviposition in insects.Acta Entomologica Sinica,52(9):1008-1016(in Chinese)[金凤,嵇保中,刘曙雯,田铃,高洁.2009.昆虫产卵分泌物的产生方式、成分及功能.昆虫学报,52(9):1008-1016]
Knolhoff LM,Heckel DG.2014.Behavioral assays for studies of host plant choice and adaptation in herbivorous insects.Annual Review of Entomology,59(1):263-278
Kostromytska OS,Rodriguez-Saona C,Alborn HT.2018.Role of plant volatiles in host plant recognition by Listronotus maculicollis,(Coleoptera:Curculionidae).Journal of Chemical Ecology,44(6):580-590
Li LC,Cao L,Li LZ,Liu X.2001.Investigating,prevention and cure the beetle damage in cold season lawn.Chinese Landscape Architecture,17(4):84-86(in Chinese)[李龙臣,曹磊,李连忠,刘晓.2001.四纹丽金龟对冷季型草坪的危害及防治.中国园林,17(4):84-86]
Lu JH,Liu SS,Shelton AM.2004.Laboratory evaluations of a wild crucifer Barbarea vulgaris as a management tool for the diamondback moth Plutella xylostella(Lepidoptera:Plutellidae).Bulletin of Entomological Research,94(6):509-516
Meiners T.2015.Chemical ecology and evolution of plant-insect interactions:a multitrophic perspective.Current Opinion in Insect Science,8:22-28
Nottingham SF,Hardie J,Dawson GW,Hick AJ,Pickett JA,Wadhams LJ,Woodcock CM.1991.Behavioral and electrophysiological responses of aphids to host and nonhost plant volatiles.Journal of Chemical Ecology,17(6):1231-1242
Shang XH.1979.Studies on the bionomics and control of Popillia quadriguttata F.Acta Entomologica Sinica,22(4):478-480(in Chinese)[商学惠.1979.四纹丽金龟发生规律和防治研究.昆虫学报,22(4):478-480]
Sun XL,Wang GC,Gao Y,Zhang XZ,Xin ZJ,Chen ZM.2014.Volatiles emitted from tea plants infested by Ectropis obliqua larvae are attractive to conspecific moths.Journal of Chemical Ecology,40(10):1080-1089
Thompson JN.1988.Evolutionary ecology of the relationship between oviposition preference and performance of offspring in phytophagous insects.Entomologia Experimentalis et Applicata,47(1):3-14
Wang W,Zhen WL.2006.Four kinds of agents prevent and control comparative test of golden beetle of plum orchard.Northern Horticulture,(6):167-168(in Chinese)[王文,甄伟玲.2006.四种药剂防治李园四纹丽金龟比较试验.北方园艺,(6):167-168]
Yan FM.2011.Chemical ecology(2nd edition).Beijing:Science Press,pp.237-399(in Chinese)[闫凤鸣.2011.化学生态学(第2版).北京:科学出版社,pp.237-399]
Bernays EA,Chapman RF.1994.Host-plant selection by phytophagous insects.//Host-plant selection by phytophagous insects.US:Springer,pp.1754-1756
Beyaert I,W?schke N,Scholz A,Varama M,Reinecke A,Hilker M.2010.Relevance of resource-indicating key volatiles and habitat odour for insect orientation.Animal Behaviour,79(5):1077-1086
Bruce TJA,Wadhams LJ,Woodcock CM.2005.Insect host location:a volatile situation.Trends in Plant Science,10(6):269-274
Cui LX,Zhang Z,Huang DZ.2010.Research progress in the effects of non-host volatiles to conifer-inhabiting bark beetles.Forest Research,23(6):895-904(in Chinese)[崔丽贤,张真,黄大庄.2010.非寄主挥发物对针叶树小蠹虫作用的研究进展.林业科学研究,23(6):895-904]
Fan LQ,Yan SC,Cheng H,Sun ZH.2012.Antennal activity and EAGresponses of Asian longhorn beetle Anoplophora glabripennis(Coleoptera:Cerambycidae)to plant terpenes.Journal of Northeast Forestry University,40(11):143-146(in Chinese)[范丽清,严善春,程红,孙宗华.2012.光肩星天牛对寄主植物萜烯类化合物触角活性和电位反应.东北林业大学学报,40(11):143-146]
Fu XX,Xu X,Lu AJ,Bi JR,Su X,Shi SS.2014.Relationship between components change of soybean volatile and Leguminivora glycinivorella infestation.Journal of Jilin Agricultural University,36(4):389-394(in Chinese)[付晓霞,徐伟,卢爱军,毕嘉瑞,苏贤,史树森.2014.大豆植株挥发物组分变化与大豆食心虫为害的关系.吉林农业大学学报,36(4):389-394]
Ge YF,Han JY,Zhou YM,Xu YM,Ding Y,Shi M,Guo CK,Wu G.2018.Silencing of mi R156 confers enhanced resistance to brown planthopper in rice.Planta,https://doi.org/10.1007/s00425-018-2942-2946
Gregg PC,Del Socorro AP,Hawes AJ,Binns MR.2016.Developing bisexual attract-and-kill for polyphagous insects:ecological rationale versus pragmatics.Journal of Chemical Ecology,42(7):666-675
Jin F,Ji BZ,Liu SW,Tian L,Gao J.2009.Deposition modes,components and functions of secretions associated with oviposition in insects.Acta Entomologica Sinica,52(9):1008-1016(in Chinese)[金凤,嵇保中,刘曙雯,田铃,高洁.2009.昆虫产卵分泌物的产生方式、成分及功能.昆虫学报,52(9):1008-1016]
Knolhoff LM,Heckel DG.2014.Behavioral assays for studies of host plant choice and adaptation in herbivorous insects.Annual Review of Entomology,59(1):263-278
Kostromytska OS,Rodriguez-Saona C,Alborn HT.2018.Role of plant volatiles in host plant recognition by Listronotus maculicollis,(Coleoptera:Curculionidae).Journal of Chemical Ecology,44(6):580-590
Li LC,Cao L,Li LZ,Liu X.2001.Investigating,prevention and cure the beetle damage in cold season lawn.Chinese Landscape Architecture,17(4):84-86(in Chinese)[李龙臣,曹磊,李连忠,刘晓.2001.四纹丽金龟对冷季型草坪的危害及防治.中国园林,17(4):84-86]
Lu JH,Liu SS,Shelton AM.2004.Laboratory evaluations of a wild crucifer Barbarea vulgaris as a management tool for the diamondback moth Plutella xylostella(Lepidoptera:Plutellidae).Bulletin of Entomological Research,94(6):509-516
Meiners T.2015.Chemical ecology and evolution of plant-insect interactions:a multitrophic perspective.Current Opinion in Insect Science,8:22-28
Nottingham SF,Hardie J,Dawson GW,Hick AJ,Pickett JA,Wadhams LJ,Woodcock CM.1991.Behavioral and electrophysiological responses of aphids to host and nonhost plant volatiles.Journal of Chemical Ecology,17(6):1231-1242
Shang XH.1979.Studies on the bionomics and control of Popillia quadriguttata F.Acta Entomologica Sinica,22(4):478-480(in Chinese)[商学惠.1979.四纹丽金龟发生规律和防治研究.昆虫学报,22(4):478-480]
Sun XL,Wang GC,Gao Y,Zhang XZ,Xin ZJ,Chen ZM.2014.Volatiles emitted from tea plants infested by Ectropis obliqua larvae are attractive to conspecific moths.Journal of Chemical Ecology,40(10):1080-1089
Thompson JN.1988.Evolutionary ecology of the relationship between oviposition preference and performance of offspring in phytophagous insects.Entomologia Experimentalis et Applicata,47(1):3-14
Wang W,Zhen WL.2006.Four kinds of agents prevent and control comparative test of golden beetle of plum orchard.Northern Horticulture,(6):167-168(in Chinese)[王文,甄伟玲.2006.四种药剂防治李园四纹丽金龟比较试验.北方园艺,(6):167-168]
Yan FM.2011.Chemical ecology(2nd edition).Beijing:Science Press,pp.237-399(in Chinese)[闫凤鸣.2011.化学生态学(第2版).北京:科学出版社,pp.237-399]