桑叶生物碱对高脂饮食小鼠的抗氧化作用
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摘要
本文旨在研究桑叶生物碱对高脂饮食小鼠抗氧化能力的影响。以含桑叶生物碱剂量分别为20、40和80mg/kg灌胃喂饲高脂饮食小鼠4、8、12、16周,用试剂盒测定各组小鼠肝脏和血清中丙二醛(malondlaldehyde,MDA)等指标。结果表明,与高脂对照组小鼠相比,以桑叶生物碱剂量为80mg/kg灌胃小鼠16周,小鼠肝脏中MDA含量减少43.63%,血清中的减少50.91%;肝脏中总抗氧化能力(total antioxidant capability,T-AOC)、还原型谷胱甘肽(glutathione,GSH)含量分别增加2.36倍、2.69倍,血清中的分别增加2.25倍、1.59倍;肝脏中过氧化氢酶(catalase,CAT)、过氧化物酶(peroxidase,POD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)活性分别增加1.82倍、2.22倍、1.90倍、1.22倍,血清中的分别增加1.48倍、2.19倍、1.59倍、1.44倍。结论,桑叶生物碱对高脂饮食小鼠具有较强的抗氧化作用。
To study the antioxidant capacity of mulberry alkaloid in mice on high fat-diet, the mice on high fat-diet were fed with alkaloid at different concentrations(20, 40, 80 mg/kg) by gastric gavage for 4, 8, 12, 16 weeks respectively, the content of malondialdehyde(MDA) and other indicators in liver and serum of the mice on high fat-diet were assessed by kit. The results showed that in the mice fed with a dose of 80 mg/kg by gastric gavage for 16 weeks, the content of MDA in liver decreased by 43.63% and in serum decreased by 50.91%, compared with the hyperlipidemic control group. The contents of T-AOC and GSH in liver were increased by 2.36 and 2.69 times respectively, and the contents in serum increased by 2.25 and 1.59 times respectively. The activity of glutathione(GSH), catalase(CAT), peroxidase(POD), glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in liver increased by 1.82, 2.22, 1.90 and 1.22 times, and the activity in serum increased by 1.48, 2.19, 1.59 and 1.44 times, respectively. In conclusion, the alkaloids of mulberry leaves have strong antioxidant effects on high-fat diet mice.
To study the antioxidant capacity of mulberry alkaloid in mice on high fat-diet, the mice on high fat-diet were fed with alkaloid at different concentrations(20, 40, 80 mg/kg) by gastric gavage for 4, 8, 12, 16 weeks respectively, the content of malondialdehyde(MDA) and other indicators in liver and serum of the mice on high fat-diet were assessed by kit. The results showed that in the mice fed with a dose of 80 mg/kg by gastric gavage for 16 weeks, the content of MDA in liver decreased by 43.63% and in serum decreased by 50.91%, compared with the hyperlipidemic control group. The contents of T-AOC and GSH in liver were increased by 2.36 and 2.69 times respectively, and the contents in serum increased by 2.25 and 1.59 times respectively. The activity of glutathione(GSH), catalase(CAT), peroxidase(POD), glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in liver increased by 1.82, 2.22, 1.90 and 1.22 times, and the activity in serum increased by 1.48, 2.19, 1.59 and 1.44 times, respectively. In conclusion, the alkaloids of mulberry leaves have strong antioxidant effects on high-fat diet mice.
引文
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[3]Parelman M A,Storms D H,Kirschke C P,et al.Dietary strawberry powder reduces blood glucose concentrations in obese and lean C57BL/6 mice,and selectively lowers plasma c-reactive protein in lean mice[J].Br J Nutr,2012,108:1789-1799
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[8]Li Y G,Ji D F,Zhong S,et al.1-deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin induced diabetic mice[J].Scientific Reports,2013,3:1377
[9]王贺.新疆药桑叶生物碱的提取及不同活性成分降血糖作用的研究[D].阿拉尔:塔里木大学,2016WANG He.Study on extract technology of alkaloids and hypoglycemic effect on different active components from Morusnigra L.in Xinjiang province[D].Alaer:Tarim University,2016
[10]卫生部卫生监督局.保健食品检验与评论技术规范实施手册[M].北京:中科多媒体电子出版社,2003Health Supervision Bureau of the Ministry of Health.Health food inspection and review technical specification implementation manual[M].Beijing:Zhongke Multimedia Electronic Publishing House,2003
[11]黄勇,刘凡,廖森泰,等.采用大孔树脂与阳离子交换树脂分离纯化桑叶活性物质的效果分析[J].蚕业科学,2012,38(6):1079-1085HUANG Yong,LIU Fan,LIAO Sen-tai,et al.Analysis on separation and purification effect of active substances from mulberry leaves with macroporous resins and cation exchange resin[J].Science of Sericulture,2012,38(6):1079-1085
[12]Cho A S,Jeon S M,Kim M J,et al.Chlorogenic acid exhibits anti-obesity property and improves lipid metabolism in high-fat diet induced obese mice[J].Food Chem Toxicol,2010,48:937-943
[13]Furukawa S,Ujita T,Himabukur O M,et al.Increased oxidative stress in obesity and its impact on metabolic syndrome[J].J Clin Invest,2004,114:1752-1762
[14]李龙囡.抗氧化功能因子对高脂膳食小鼠脂代谢的调节作用及其机制研究[D].无锡:江南大学,2013LI Long-nan.Effect and mechanisms of antioxidant factors on liquid metabolism in high-fat diet-fed mice[D].Wuxi:Jiangnan University,2013
[15]綦文涛,王世霞,李笑蕊,等.荞麦粉对高脂血症小鼠血脂和肝脏抗氧化功能的调节作用[J].中国食品学报,2018,2:63-70QI Wen-tao,WANG Shi-xia,LI Xiao-rui,et al.Regulation function of buckwheat on blood lipid and liver anti-oxidation of hyperlipemia mice[J].Journal of Chinese Institute of Food Science and Technology,2018,2:63-70
[16]Hao J Y,Wan Y,Yao X H,et al.Effect of different planting areas on the chemical compositions and hypoglycemic and antioxidant activities of mulberry leaf extracts in southern China[J].Plos One,2018,26:1-15
[17]Valko M,Leibfritz D,Moncol J,et al.Free radicals and antioxidants in normal physiological functions and human disease[J].Int J Biochem Cell Biol,2007,39:44-84
[18]Meng Q,Shi D,Feng J,et al.Hypercholesterolemia up-regulates the expression of intermedin and its receptor componentsin the aorta of rats via inducing the oxidative stress[J].Annals of Clinical&Laboratory Science,2016,46(1):5-17
[19]Adegbola P,Aderibigbe I,Hammed W,et al.Antioxidant and anti-inflammatory medicinal plants have potential role in the treatment of cardiovascular disease:A review[J].American Journal of Cardiovascular Disease,2017,7(2):19-32
[20]高璐,王滢,饶胜其,等.葡萄籽原花青素提取物对衰老模型小鼠抗氧化作用[J].食品科学,2014,35(23):253-256GAO Lu,WANG Ying,RAO Sheng-qi,et al.Antioxidant activity of grape seed proanthocyanidin extract in aging mouse model[J].Food Science,2014,35(23):253-256
[21]刘富萍,张琦,谷楠,等.谷胱甘肽及丙二醛在炎症中表达的研究进展[J].医学美学美容旬刊,2015,24(6):1001-1002LIU Fu-ping,ZHANG Qi,GU Nan,et al.Advances in the expression of glutathione and malondialdehyde in inflammation[J].Medical Aesthetics and Beauty Magazine,2015,24(6):1001-1002
[22]Yang F,Zhang C,Zhuang Y,et al.Oxidative stress and cell apoptosis in caprine liver induced by molybdenum and cadmium in combination[J].Biol Trace Elem Res,2016,173(1):9-86
[23]王美菊,陶明煊,牛文颖,等.石榴叶多酚对急性酒精性肝损伤小鼠肾脏、心脏及免疫器官的抗氧化作用[J].食品科学,2016,37(1):208-213WANG Mei-ju,TAO Ming-xuan,NIU Wen-ying,et al.Antioxidant effects of purified polyphenols from leaves of Punicagranatum L.on kidney,heart and immune organs in mice with acute alcoholic liver injury[J].Food Science,2016,37(1):208-213
[24]刘稳,李杨,高培基,等.过氧化物酶研究进展[J].纤维素科学与技术,2000,2(9):52-64LIU Wen,LI Yang,GAO Pei-ji,et al.Research advance in peroxidases[J].Journal of Cellulose Science and Technology,2000,2(9):52-64
[25]董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技导报,2013,5(15):53-58DONG Liang,HE Yong-zhi,WANG Yuan-liang,et al.Research progress on application of superoxide dismutase(SOD)[J].Journal of Agricultural Science and Technology,2013,5(15):53-58
[26]Karakiulakis G,Roth M.Muscarinic receptors and their antagonistsin COPD:Anti-inflammatory and antiremodeling effects[J].Mediators Inflamm,2012,2012:1-9
[27]程光宇,刘俊,王峰,等.鸡腿菇子实体多糖对小鼠血液和肝脏的抗氧化作用[J].食品科学,2010,31(13):267-272CHENG Guang-yu,LIU Jun,WANG Feng,et al.Antioxidant effects of coprinus comatus polysaccharides in the blood and liver of normal and alloxan-induced diabetic mice[J].Food Science,2010,31(13):267-272
[28]Nordgren,Marcus,Fransen Marc,et al.Peroxisomal metabolism and oxidative stress[J].Biochimie,2014,98(3):56-64
[29]王富明,张亚敏,孙华,等.针刺对大鼠脑缺血再灌注损伤后不同时间点血清SOD和MDA表达的影响[J].针灸临床杂志,2015,31(2):62-65WANG Fu-ming,ZHANG Ya-min,SUN Hua,et al.Expression of SOD and MDA in the serum of rats subjected to cerebral ischemic-reperfusion treated by acupuncture[J].Journal of Clinical Acupuncture and Moxibustion,2015,31(2):62-65
[30]Chung S S,Kim M,Youn B S,et al.Glutathione peroxidase 3mediates the antioxidant effect of peroxisome proliferator-activated receptorγin human skeletal muscle cells[J].Molecular and Cellular Biology,2009,29(1):20-30
[31]Meigs J B,Larson M G,Fox C S,et al.Association of oxidative stress,insulin resistance,and diabetes risk phenotypes the framingham offspring study[J].Diabetes Care,2007,30(10):2529-2535
[32]张伟.青砖茶对实验大鼠的减肥和调节血脂作用及其机制研究[D].武汉:华中农业大学,2009ZHANG Wei.Studies on anti-obesity,blood liquid regulation effects and mechanisms of dark brick tea on rats[D].Wuhan:Huazhong Agriculture University,2009
[33]Hu X Q,Thakur K,Chen G H,et al.Metabolic effect of1-deoxynojirimycin from mulberry leaves on db/db diabetic mice using liquid chromatography-mass spectrometry based metabolomics[J].Journal of Agricultural and Food Chemistry,2017,65(23):4658-4667
[34]Takahata Y,Ohnishi K M,Furuta S,et al.Highly polymerized procyanidins in brown soybean seed coat with a high radical-scavenging activity[J].Journal of Agricultural and Food Chemistry,2001,49(12):5843-5847
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