一种钇金属配合物的合成、表征及DNA性质的研究
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摘要
选用2,2'-联吡啶-4,4'-二甲酸配体与钇金属,在100℃水热条件反应获得一个新颖的钇配合物Y_2(H_2bpydc)_3·H_2O.利用元素分析、X-射线单晶衍射对新颖钇配合物结构进行结构表征,并通过荧光光谱法测定了钇配合物与鱼精DNA的结合能力.通过凝胶电泳技术分析化合物切割pBR322质粒DNA的能力.荧光光谱显示出该钇配合物有良好的DNA性质,而且凝胶电泳测定也证明复合物切割pBR322质粒DNA的有效切割能力.研究结果表明:该配位聚合物对靶向DNA有极强的结合能力,并对肿瘤细胞有极强的抑制活性,是一种潜在的新型无机抗肿瘤聚合物.
In this paper,a novel ruthenium complex Y_2(H_2bpydc)_3·H_2O was obtained by reacting 2,2'-bipyridyl-4,4'-dicarboxylic acid ligand with ruthenium metal under hydrothermal conditions at 100℃.The structure of the novel ruthenium complex was characterized by elemental analysis and X-ray single crystal diffraction. The binding ability of the ruthenium complex and fish sperm DNA was determined by fluorescence spectroscopy. The ability of the compound to cleave pBR322 plasmid DNA was analyzed by gel electrophoresis. Good DNA properties of the ruthenium complex were showed by fluorescence spectroscopy,a nd gel electrophoresis assays also demonstrated the ability of the complex to cleave pBR322 plasmid DNA. The results showed that the coordination polymer had strong binding ability to target DNA and strong inhibitory activity on tumor cells. It is a potential new inorganic antitumor polymer.
In this paper,a novel ruthenium complex Y_2(H_2bpydc)_3·H_2O was obtained by reacting 2,2'-bipyridyl-4,4'-dicarboxylic acid ligand with ruthenium metal under hydrothermal conditions at 100℃.The structure of the novel ruthenium complex was characterized by elemental analysis and X-ray single crystal diffraction. The binding ability of the ruthenium complex and fish sperm DNA was determined by fluorescence spectroscopy. The ability of the compound to cleave pBR322 plasmid DNA was analyzed by gel electrophoresis. Good DNA properties of the ruthenium complex were showed by fluorescence spectroscopy,a nd gel electrophoresis assays also demonstrated the ability of the complex to cleave pBR322 plasmid DNA. The results showed that the coordination polymer had strong binding ability to target DNA and strong inhibitory activity on tumor cells. It is a potential new inorganic antitumor polymer.
引文
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