合欢花饮片标准汤剂质量标准评价的建立
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摘要
目的:制备合欢花饮片标准汤剂,测定合欢花标准汤剂中槲皮苷的含量,并建立指纹图谱,以进行质量研究。方法:依照标准汤剂的制备要求,制备15批不同产地的合欢花饮片标准汤剂,以槲皮苷作为定量检测指标,计算转移率与出膏率,并建立其UPLC特征图谱分析方法。结果:通过对15批合欢花标准汤剂进行测定,槲皮苷转移率为72.33%～41.51%,出膏率为26.6%～23.4%;并用中药色谱指纹图谱相似度评价系统软件进行特征图谱分析,标定了其中5个共有峰,对15批合欢花饮片标准汤剂分别进行了相似度评价,其相似度均大于0.90。结论:合欢花饮片标准汤剂制备规范,测定方法精密度、稳定性和重复性良好,可为合欢花配方颗粒的质量控制提供参考。
Objective:To prepare Flos Albiziae standard decoction and determine the content of quercetin in standard decoction and establish chromatographic fingerprint for studying the quality standard decoction.Methods:Fifteen batches of Flos Albiziae standard decoctions were prepared according to the standardization method.With quercetin as the detection index,the transfer rate and extraction rate were calculated and a UPLC characteristic chromatograms analysis method was established.Results:According to the measurement of 15 batches of standard decoctions,the transfer rate ranged from 72.33%to41.51%and extract rate was at the range of 26.6% to 23.4%.Besides,the Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine TCM was used to analyze and compare the characteristic chromatograms,five peaks were calibrated.Moreover,the similarity of fifteen batches was over 0.90.Conclusion:Samples were prepared by a standard method.The method displayed a good precision stability and repeatability.Therefore,this study can provide a reference for the quality control of Flos Albiziae formula granules.
Objective:To prepare Flos Albiziae standard decoction and determine the content of quercetin in standard decoction and establish chromatographic fingerprint for studying the quality standard decoction.Methods:Fifteen batches of Flos Albiziae standard decoctions were prepared according to the standardization method.With quercetin as the detection index,the transfer rate and extraction rate were calculated and a UPLC characteristic chromatograms analysis method was established.Results:According to the measurement of 15 batches of standard decoctions,the transfer rate ranged from 72.33%to41.51%and extract rate was at the range of 26.6% to 23.4%.Besides,the Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine TCM was used to analyze and compare the characteristic chromatograms,five peaks were calibrated.Moreover,the similarity of fifteen batches was over 0.90.Conclusion:Samples were prepared by a standard method.The method displayed a good precision stability and repeatability.Therefore,this study can provide a reference for the quality control of Flos Albiziae formula granules.
引文
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[13] 彭丽华,赖智填,成金乐,等.基于HPLC指纹图谱的中药破壁饮片质量评价[J].中国现代中药,2016,18(9):1230-1232.
[2] 施学丽,郭超峰.合欢花的研究进展[J].中国民族医药杂志,2012,7(12):30-31.
[3] 康健,李作平,沈立茹.正交实验法优选合欢花提取工艺的研究[J].中成药,2007,29(6):902-904.
[4] 耿红梅.合欢花黄酮类化学成分的研究[J].衡水学院学报,2011,13(1):28-29.
[5] 田硕,苗明三.合欢花现代研究分析[J].中医学报,2014,29(6):859-861.
[6] 袁建梅,郭伟云,汪应灵.合欢花中总黄酮的提取工艺及对羟自由基清除作用的研究[J].中国食品添加剂,2012(1):87-91.
[7] 肖小河,鄢丹,马丽娜,等.中药现代化研究近十年概论[J].中国现代中药,2012,14(1):7-12.
[8] 乔彬,张琛,张建春.中药配方颗粒的应用现状及前景分析[J].甘肃科技,2017,3(21):127-130.
[9] 国家药典委员会.中药配方颗粒重量控制与标准制定技术要求(征求意见稿)[S].北京:中医药科技出版社,2016:1-14.
[10] 陈士林,刘安,李琦,等.中药饮片标准汤剂研究策略[J].中国中药杂志,2016,41(8):1367-1375.
[11] 李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药,2013,44(22):3095-3104.
[12] 方德敏.中药指纹图谱技术的研究及应用[J].中草药,2005,36(4):236-237.
[13] 彭丽华,赖智填,成金乐,等.基于HPLC指纹图谱的中药破壁饮片质量评价[J].中国现代中药,2016,18(9):1230-1232.