比色法测定巴西人参中齐墩果酸型皂苷含量
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摘要
目的建立测定巴西人参中齐墩果酸型皂苷含量的比色法。方法以人参皂苷Ro和齐墩果酸作为对照品,以5%香草醛-冰醋酸-高氯酸作为显色体系,通过比色法测定皂苷的含量。结果皂苷比色法与苷元比色法均可用于巴西人参中皂苷含量的测定,齐墩果酸型皂苷含量分别为9.956%和3.232%(n=5)。结论苷元比色法简便、快速,重复性好,测定结果较皂苷比色法更合理、准确。
Objective To establish a colorimetry for the content determination of oleanolic acid-type saponins in Pfaffia paniculata.Methods With ginsenoside Ro and oleanolic acid as the reference substance,and 5% vanillin-glacial acetic acid-perchloric acid as the chromogenic system, the content of saponins was determined by colorimetry.Results Saponin colorimetry and aglycone colorimetry were both suitable for the content determination of saponins in Pfaffia paniculata.The content of oleanolic acid-type saponins obtained by the two methods were 9.956% and 3.232%(n = 5),respectively.Conclusion The aglycone colorimetry is simple,rapid and reproducible,and the results of determination are more reasonable and accurate than those of saponin colorimetry.
Objective To establish a colorimetry for the content determination of oleanolic acid-type saponins in Pfaffia paniculata.Methods With ginsenoside Ro and oleanolic acid as the reference substance,and 5% vanillin-glacial acetic acid-perchloric acid as the chromogenic system, the content of saponins was determined by colorimetry.Results Saponin colorimetry and aglycone colorimetry were both suitable for the content determination of saponins in Pfaffia paniculata.The content of oleanolic acid-type saponins obtained by the two methods were 9.956% and 3.232%(n = 5),respectively.Conclusion The aglycone colorimetry is simple,rapid and reproducible,and the results of determination are more reasonable and accurate than those of saponin colorimetry.
引文
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[11]袁丁,何毓敏,鲁科明,等.中药竹节参中总皂苷和齐墩果酸的含量测定[J].西北药学杂志,2008,23(5):278-280.
[12]李成刚,张永忠,迟玉节,等.齐墩果酸分光光度法测定大豆异黄酮粉中总皂苷的含量[J].东北农业大学学报,2008,39(7):13-16.
[13]师文添,于学雷,袁建,等.苷元比色法测定大豆总皂苷[J].食品科学,2009,30(2):211-214.
[14]陈旭,党晓芳,曹飒丽,等.三七中总皂苷含量测定的对照品筛选[J].中国实验方剂学,2013,19(7):66-68.
[15]凌征柱,蓝祖栽,马小军,等.巴西人参的引种栽培研究[J].国外医药:植物药分册,2007,22(5):204-205.
[2]WATANABE T,WATANABE M,WATANABE Y,et al.Effects of Oral Administration of Pfaffia paniculata(Brazilian Ginseng)on Incidence of Spontaneous Leukemua in AKR/J Mice[J].Cancer Detecttion and Prevention,2000,24(2):173-178.
[3]高辉,马小军,温学森,等.珐菲亚属植物化学成份和药理活性研究进展[J].中国中药杂志,2006,31(21):1749.
[4]曲正义,刘宏群,郑培和,等.齐墩果酸型皂苷药理活性研究[J].中成药,2012,34(6):1143-1147.
[5]王佳,郑培和,许世泉,等.人参、西洋参不同部位中齐墩果酸型皂苷含量的对比分析[J].特产研究,2015,37(2):23-29.
[6]白吉庆,王小平,盛艳,等.不同产地对九节菖蒲中总皂苷、齐墩果酸及腺苷含量的影响[J].中国药业,2012,21(11):9-10.
[7]高辉,马小军,温学森,等.巴西人参的化学成分研究[J].中草药,2009,40(4):522-525.
[8]刘伟锐,张霞,姜蕊,等.紫外可见分光光度法测定苣荬菜根部总三萜皂苷的含量[J].西北药学杂志,2014,29(6):554-557.
[9]桂双英,周亚球.比色法测定人参中人参总皂苷的含量[J].安徽中医学院学报,2003,22(4):51-52.
[10]孙乐,李春梅,郭莹莹,等.人参花中总皂苷的提取工艺优化[J].中国实验方剂学,2015,21(1):21-23.
[11]袁丁,何毓敏,鲁科明,等.中药竹节参中总皂苷和齐墩果酸的含量测定[J].西北药学杂志,2008,23(5):278-280.
[12]李成刚,张永忠,迟玉节,等.齐墩果酸分光光度法测定大豆异黄酮粉中总皂苷的含量[J].东北农业大学学报,2008,39(7):13-16.
[13]师文添,于学雷,袁建,等.苷元比色法测定大豆总皂苷[J].食品科学,2009,30(2):211-214.
[14]陈旭,党晓芳,曹飒丽,等.三七中总皂苷含量测定的对照品筛选[J].中国实验方剂学,2013,19(7):66-68.
[15]凌征柱,蓝祖栽,马小军,等.巴西人参的引种栽培研究[J].国外医药:植物药分册,2007,22(5):204-205.