香叶挥发油成分分析及其对小鼠白色假丝酵母菌阴道病的影响

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英文篇名：Components of Chinese medicine geranium volatile oil and its effect on the biological membrane of Candida albicans 作者：张燕 ; 邢天容 ; 王艳秋 ; 吕敬媛 ; 宋继荣 ; 胡玉红 ; 杜佳秋 ; 吴英杰 ; 佟利波 ; 卢北燕 ; 冯晓娜 ; 杨光 ; 李佳琳 ; 张伟娜 ; 殷实 ; 王琳 英文作者：ZHANG Yan;XING Tianrong;WANG Yanqiu;LYU Jingyuan;SONG Jirong;HU Yuhong;DU Jiaqiu;WU Yingjie;TONG Libo;LU Beiyan;FENG Xiaona;YANG Guang;LI Jialin;ZHANG Weina;YIN Shi;WANG Lin;The First Affiliated Hospital of Jiamusi University; 关键词：香叶挥发油 ; 白念珠菌 ; 中药 ; 细菌生物膜 英文关键词：Volatile oil;;Candida albicans;;Chinese medicine;;Biofilm 中文刊名：ZGWS 英文刊名：Chinese Journal of Microecology 机构：佳木斯大学附属第一医院;佳木斯市中心医院; 出版日期：2019-06-15 出版单位：中国微生态学杂志 年：2019 期：v.31 基金：黑龙江省教育厅科学技术研究项目(12531719) 语种：中文; 页：ZGWS201906003 页数：5 CN：06 ISSN：21-1326/R 分类号：14-17+23

摘要

目的 研究香叶挥发油对白色假丝酵母生物膜形成的影响。方法 水蒸气蒸馏法提取香叶挥发油,GC-MS分析其成分,测定其对白色假丝酵母的MIC、MBC,激光共聚焦显微镜下观察白色假丝酵母生物膜的形成,制备小鼠白色假丝酵母菌阴道病模型,观察香叶油治疗效果。结果 香叶挥发油提取率为1.83%,分析出22个成分,主要成分为5-甲基-2-异丙烯基环己酮、3,7-二甲基-2-辛烯-1-醇、(顺)香叶醇、异香叶醇、香叶醇和甲酸香茅酯,MIC值为0.3625%,MBC值为0.6250%,激光共聚焦结果显示不同浓度香叶油均可显著抑制白色假丝酵母生物膜形成,动物实验结果显示香叶油可抑制小鼠阴道炎症改变。结论 香叶油用于治疗白色假丝酵母引起的阴道炎机制可能与其影响细菌生物膜形成有关。
        Objective To study the effects of volatile oil of geranium on the formation of Candida albicans biofilm. Methods The geranium oil was extracted with steam distillation. The components were analyzed with GC-MS. The MIC and MBC of geranium volatile oil to Candida were determined. The formation of Candida albicans biofilm was observed under laser confocal microscopy. Mouse models of Candida albicans-associated vaginitis were prepared to observe the therapeutic effect of geranium oil. Results The extraction rate of geranium oil was 1.83% and 22 components were analyzed. The main components were 5-methyl-2-isopropenylcyclohexanone, 3,7-dimethyl-2-octene-1-ol,(cis)geraniol, iso-geraniol, geraniol and citronellyl formate. The MIC value was 0.3625%, and MBC value was 0.6250%. Laser confocal results showed that different concentrations of geranium oil significantly inhibited the formation of Candida albicans biofilm. Animal experiments showed that geranium oil inhibited vaginal inflammation in mice. Conclusion Geranium oil can be used to treat vaginitis caused by Candida albicans. The mechanism may be related to its inhibitory effect on the formation of bacterial biofilm.

引文

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