survivin和Ki-67蛋白在喉癌患者中的表达情况及与临床特征的关系
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摘要
目的探讨存活蛋白(survivin)和Ki-67在喉癌患者中的表达情况及其与临床特征的关系。方法收集喉癌组织标本120例及癌旁组织标本50例,采用免疫组织化学法检测喉癌组织和癌旁组织中survivin和Ki-67蛋白的表达情况,分析喉癌组织中survivin和Ki-67蛋白表达与临床特征的关系。结果喉癌组织中survivin和Ki-67蛋白的阳性表达率均明显高于癌旁组织(P﹤0.01)。不同年龄、性别、分化程度的喉癌患者喉癌组织中survivin和Ki-67蛋白的阳性表达率比较,差异均无统计学意义(P﹥0.05)。TNM分期为Ⅲ～Ⅳ期、有淋巴结转移的喉癌患者喉癌组织中survivin和Ki-67蛋白的阳性表达率均明显高于TNM分期为Ⅰ～Ⅱ期、无淋巴结转移的患者,差异均有统计学意义(P﹤0.01)。结论 survivin和Ki-67蛋白在喉癌组织中高表达,并且与喉癌的发生发展关系密切。
Objective To investigate the expression of survivin and Ki-67 in laryngeal cancer patients and the relationship with clinical characteristics. Method The laryngeal cancer tissues were collected from 120 cases of laryngeal cancer, meanwhile the adjacent tissues surrounding the lesions were collected from 50 cases. Immunohistochemical assay was used to detect the expression of survivin and Ki-67 protein in the laryngeal cancer tissues and adjacent tissues, and the relationship between expression of survivin and Ki-67 protein and clinical characteristics was investigated. Result The positive expression rate of survivin and Ki-67 protein in the laryngeal cancer tissues was significantly higher than that in adjacent tissues(P<0.01). The positive expression rate of survivin and Ki-67 protein were independent of age, gender and degree of differentiation in laryngeal cancer patients(P>0.05), while the positive expression rate of survivin and Ki-67 in laryngeal cancer tissues with TNM stage Ⅱ-Ⅱ and with lymph node metastasis were significantly higher than those with TNM stage Ⅱ-Ⅱand without lymph node metastasis(P<0.01). Conclusion survivin and Ki-67 protein are highly expressed in laryngeal cancer tissues, and are closely related to the occurrence and development of laryngeal cancer.
Objective To investigate the expression of survivin and Ki-67 in laryngeal cancer patients and the relationship with clinical characteristics. Method The laryngeal cancer tissues were collected from 120 cases of laryngeal cancer, meanwhile the adjacent tissues surrounding the lesions were collected from 50 cases. Immunohistochemical assay was used to detect the expression of survivin and Ki-67 protein in the laryngeal cancer tissues and adjacent tissues, and the relationship between expression of survivin and Ki-67 protein and clinical characteristics was investigated. Result The positive expression rate of survivin and Ki-67 protein in the laryngeal cancer tissues was significantly higher than that in adjacent tissues(P<0.01). The positive expression rate of survivin and Ki-67 protein were independent of age, gender and degree of differentiation in laryngeal cancer patients(P>0.05), while the positive expression rate of survivin and Ki-67 in laryngeal cancer tissues with TNM stage Ⅱ-Ⅱ and with lymph node metastasis were significantly higher than those with TNM stage Ⅱ-Ⅱand without lymph node metastasis(P<0.01). Conclusion survivin and Ki-67 protein are highly expressed in laryngeal cancer tissues, and are closely related to the occurrence and development of laryngeal cancer.
引文
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[10] Mc Kenzie JA, Liu T, Goodson AG, et al. Survivin enhances motility of melanoma cells by supporting Akt activation andα5 integrin upregulation[J]. Cancer Res, 2010, 70(20):7927-7937.
[11]金爱红,周霞平,周凤珍. Snail及Survivin在卵巢癌中的表达与侵袭性相关性分析[J].重庆医学, 2016, 45(16):2244-2245.
[12]王娜,徐小艳,申洪,等. NEDD9、Ki67、P53联合检测对胃癌的早期诊断和恶性程度评估的价值[J].实用医学杂志, 2017, 33(12):1961-1965.
[13]杨光,赵佳佳,陈晏,等. Bax、Survivin及Ki67在喉癌中的表达及意义[J].临床和实验医学杂志, 2017, 16(14):1360-1363.
[14]王菊香,杨宝良,裴士庚,等. Survivin与p53及Ki67蛋白在喉癌组织中的表达及相关性[J].临床耳鼻咽喉头颈外科杂志, 2015, 28(17):1545-1548.
[2]白雪.喉癌根治术医院感染的预防性护理要点分析[J].医药前沿, 2016, 6(20):219-220.
[3] Wen LJ, Gao LF, Jin CS, et al. Small interfering RNA survivin and GRIM-19 co-expression salmonella plasmid inhibited the growth of laryngeal cancer cells in vitro and in vivo[J]. Int J Clin Exp Pathol, 2013, 6(10):2071-2081.
[4] Zhao H, Ren J, Zhuo X, et al. Prognostic significance of Survivin and CD44v6 in laryngeal cancer surgical margins[J]. J Cancer Res Clin Oncol, 2008, 134(10):1051-1058.
[5]胡慈浩,王文华,王全,等. Survivin和Aurora-b在喉癌组织中的表达及其临床意义[J].临床耳鼻咽喉头颈外科杂志, 2017, 30(10):737-743.
[6]陈文杰,王斌全,高伟,等.喉癌流行病学特征及影响因素分析[J].中国当代医药, 2015, 32(12):43-46.
[7]刘红兵,刘月辉,罗英,等.喉癌的手术方式选择及远期疗效的分析[J].中国癌症杂志, 2015, 25(2):145-149.
[8]马泓智,冯凌,李平栋,等.中晚期喉癌颈淋巴结转移规律相关因素分析[J].中华肿瘤防治杂志, 2015, 22(7):542-547.
[9] Altieri DC. Survivin-the inconvenient IAP[J]. Semin Cell Dev Biol, 2015, 39:91-96.
[10] Mc Kenzie JA, Liu T, Goodson AG, et al. Survivin enhances motility of melanoma cells by supporting Akt activation andα5 integrin upregulation[J]. Cancer Res, 2010, 70(20):7927-7937.
[11]金爱红,周霞平,周凤珍. Snail及Survivin在卵巢癌中的表达与侵袭性相关性分析[J].重庆医学, 2016, 45(16):2244-2245.
[12]王娜,徐小艳,申洪,等. NEDD9、Ki67、P53联合检测对胃癌的早期诊断和恶性程度评估的价值[J].实用医学杂志, 2017, 33(12):1961-1965.
[13]杨光,赵佳佳,陈晏,等. Bax、Survivin及Ki67在喉癌中的表达及意义[J].临床和实验医学杂志, 2017, 16(14):1360-1363.
[14]王菊香,杨宝良,裴士庚,等. Survivin与p53及Ki67蛋白在喉癌组织中的表达及相关性[J].临床耳鼻咽喉头颈外科杂志, 2015, 28(17):1545-1548.