一株拮抗稻瘟病内生链霉菌OsiSh-10的筛选与鉴定
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摘要
从湖南浏阳大围山种植的湘矮早7号水稻中,筛选到一株对水稻稻瘟病菌有显著抑制效果的水稻内生放线菌OsiSh-10菌株.由拮抗菌包覆种子和喷晒水稻叶面的实验表明将该菌喷晒于水稻叶面可以对叶瘟起到明显的控制效果,且喷一次的效果比喷两次的效果好,包覆种子反而使病情指数升高.根据形态特征、培养特征、生理生化、16s rRNA序列比对及进化树构建鉴定OsiSh-10菌株属于白色链霉菌(Streptomyces albus).OsiSh-10是一株具有稻瘟病生防潜力的菌株.
An endophytic actinomycete strain OsiSh-10 significantly against rice blast pathogens was successfully isolated from Xiangaizao 7rice(Oryza indica)of Liuyang Dawei Mountain in Hunan Province.The control effects of OsiSh-10 on leaf blast in field were investigated by coating seeds and spraying leaf of rice with it,which were as follows:leaf blast was controlled by spraying OsiSh-10 on leaf of rice,but coated seeds with OsiSh-10 unworked for leaf blast.According to its morphological features,culture characteristics,physiological and biochemical characteristics,16 srRNA gene sequence comparison,and phylogenetic tree construction analysis,OsiSh-10 strain was classified as Streptomyces albus.OsiSh-10 strain is a promising biocontrol actinomycete for rice blast.
An endophytic actinomycete strain OsiSh-10 significantly against rice blast pathogens was successfully isolated from Xiangaizao 7rice(Oryza indica)of Liuyang Dawei Mountain in Hunan Province.The control effects of OsiSh-10 on leaf blast in field were investigated by coating seeds and spraying leaf of rice with it,which were as follows:leaf blast was controlled by spraying OsiSh-10 on leaf of rice,but coated seeds with OsiSh-10 unworked for leaf blast.According to its morphological features,culture characteristics,physiological and biochemical characteristics,16 srRNA gene sequence comparison,and phylogenetic tree construction analysis,OsiSh-10 strain was classified as Streptomyces albus.OsiSh-10 strain is a promising biocontrol actinomycete for rice blast.
引文
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[2]KHUSH G S.What it will take to feed 5.0billion rice consumer in 2030[J].Plant Molecular Biology,2005,59(1):1-6.
[3]SCHEUERMANN K K,RAIMONDI J V,MARSCHALEK R,et al.Magnaporthe oryzae genetic diversity and its outcomes on the search for durable resistance[J].Agricultural and Biological Sciences,2012,3(30):331-356.
[4]温小红,谢明杰,姜健,等.水稻稻瘟病防治方法研究进展[J].中国农学通报,2013,29(3):190-195.WEN Xiao-hong,XIE Ming-jie,JIANG Jian,et al.Advances in research on control method of rice blast[J].Chinese Agricultural Science Bulletin,2013,29(3):190-195.(In Chinese)
[5]SHAN Hong-ying,ZHAO Ming-min,CHEN De-xin,et al.Biocontrol of rice blast by the phenaminom ethylacetic acid producer of Bacillus methylotrophicus strain BC79[J].Crop Protection,2013,44(2):29-37.
[6]闫梦红,蔡正求,韩继刚,等.植物内生细菌在防治植物病害中的应用研究[J].生物技术通报,2004(3):8-12.YAN Meng-hong,CAI Zheng-qiu,HAN Ji-gang,et al.The applied research of endophytic bacteria in biological control of plant disease[J].Biotechnology Bullietin,2004(3):8-12.(In Chinese)
[7]SUADA I K,CHUN K W,CHA J Y,et al.Ability of endophytic fungi isolated from rice to inhibit Pyricularia oryzaeinduced rice blast in indonesia[J].Journal of the Faculty of Agriculture Kyushu University,2012,57(1):51-53.
[8]YASUDA M,SHINOZAKI S,ISAEA T,et al.Effects of colonization of a bacterial endophyte,Azospirillum sp B510,on disease resistance in Rice[J].Bioscience,Biotechnology and Biochemistry,2009,73(12):2595-2599.
[9]YU Hong-sheng,ZHANG Lei,LIN Li,et al.Recent developments and future prospects of antimicrobial metabolites produced by endophytes[J].Microbiological Research,2010,165(6):437-449.
[10]SESSITSCH A,HARDOIM P,DORING J,et al.Functional characteristics of an endophyte community colonizing rice roots as revealed by metagenomic analysis[J].Molecular Plant-Microbe Interactions,2012,25(1):28-36.
[11]ZHU Bo,LIU He,TIAN Wen-xiao,et al.Genome sequence of Stenotrophomonas maltophilia RR-10,isolated as an endophyte from rice root[J].Journal of Bacteriology,2012,194(5):1280-1281.
[12]YUAN Zhi-lin,SU Zhen-zhu,MAO Li-jian,et al.Distinctive endophytic fungal assemblage in stems of wild rice(Oryza granulata)in China with special reference to two species of Muscodor(Xylariaceae)[J].The Journal of Microbiology,2011,49(1):15-23.
[13]MAHAPATRA S,BANERJEE D.Diversity and screening for antimicrobial activity of endophytic fungi from alstonia scholaris[J].Acta Microbiologica and Immunologica Hungarica,2010,57(3):215-223.
[14]SPENCE C,ALFF E,JOHNSON C,et al.Nature rice rhizospheric microbes suppress rice blast infection[J].BioMedCentral Plant Biology,2014,14:130-146.
[15]SAIKIA R,GOGOI D K,YADAV A,et al.Brevibacillus laterosporus strain BPM3,apotential biocontrol agent isolated from a natural hot water spring of Assam,India[J].Microbiological Research,2011,166(3):216-225.
[16]SU Zhen-zhu,MAO Li-juan,LI Na,et al.Evidence for biotrophic lifestyle and biocontrol potential of dark septate endophyte Harpophora oryzae to Rice Blast disease[J].Plos One,2013,8(4):e61332.
[17]蒋怡,唐蜀昆,张玉琴,等.放线菌产生的生物活性物质[J].微生物学通报,2007,34(1):188-190.JIANG Yi,TANG Shu-kun,ZHANG Yu-qin,et al.Biological active substance produced by actinomycetes[J].Microbiology Bulletin,2007,34(1):188-190.(In Chinese)
[18]KAEWKLA O,FRANCO C M.Rational approaches to improving the isolation of endophytic actinobacteria from australian native trees[J].Microbiology Ecology,2013,65(2):384-393.
[19]XIONG Xian-qiu,LIAO Hong-dong,MA Jiang-shan,et al.Isolation of a rice endophytic bacterium,Pantoea sp.Sd-1,with ligninolytic activity and characterization of its rice straw degradation ability[J].Letters in Applied Microbiology,2014,58(2):123-129.
[20]SHIRLING E B,GOTTLIEB D.Methods for characterization of Streptomyces species[J].International Journal of Systematic Bacteriology,1966,16(3):313-340.
[21]宋尚直.关于白色链霉菌的筛选与鉴定[J].微生物学通报,1979,5(1):49-52.SONG Shang-zhi.Screening and indentification of Streptomyces albus[J].Microbiology Bulletin,1979,5(1):49-52.(In Chinese)
[22]FELSENSTEIN J.Confidence limits on phylogenies:an approach using the bootstrap[J].Evolution,1985,39(4):783-791.