内生白色链霉菌OsiSh-10对拟南芥根系结构的影响
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摘要
内生菌与宿主植物之间存在着复杂的相互作用。为了研究内生放线菌OsiSh-10对植物的影响,本研究构建了带荧光标记的OsiSh-10-GFP菌株,发现其主要定植于拟南芥的根部表皮细胞。将内生菌OsiSh-10与拟南芥进行共培养,发现该菌能抑制拟南芥初生根的生长并促进侧根形成。液质联用色谱分析结果显示,内生菌OsiSh-10能够分泌激动素。直接利用激动素处理与利用内生菌OsiSh-10处理的拟南芥根毛长度和密度有相似的表型,显示内生菌OsiSh-10可能通过产生激动素来影响拟南芥根系结构的形成,实验结果为研究内生放线菌与宿主植物的相互作用提供了一个新的思路。
Complicated interactions existed between endophytes and their plant hosts.In order to explore the effects of endophytic actinomyce OsiSh-10 on its host plant,we established a fluorescent labeled OsiSh-10-GFP strain and found that OsiSh-10-GFP was mainly located in the root epidermal cells of Arabidopsis thaliana.We co-cultured OsiSh-10 and Arabidopsis thaliana,and found that the OsiSh-10 could inhibit primary root growth and promote lateral root formation in Arabidopsis thaliana.The results of HPLC-MS showed that OsiSh-10 could secrete kinetin.The length and density of Arabidopsis thaliana root hair treated directly with kinetin and treated with OsiSh-10 had similar phenotypes,which hinted that OsiSh-10 might impact the formation of Arabidopsis thaliana root architecture through generating kinetin.The results of the study might provide a new way to study the interaction between endophytic antinomyces and their host plants.
Complicated interactions existed between endophytes and their plant hosts.In order to explore the effects of endophytic actinomyce OsiSh-10 on its host plant,we established a fluorescent labeled OsiSh-10-GFP strain and found that OsiSh-10-GFP was mainly located in the root epidermal cells of Arabidopsis thaliana.We co-cultured OsiSh-10 and Arabidopsis thaliana,and found that the OsiSh-10 could inhibit primary root growth and promote lateral root formation in Arabidopsis thaliana.The results of HPLC-MS showed that OsiSh-10 could secrete kinetin.The length and density of Arabidopsis thaliana root hair treated directly with kinetin and treated with OsiSh-10 had similar phenotypes,which hinted that OsiSh-10 might impact the formation of Arabidopsis thaliana root architecture through generating kinetin.The results of the study might provide a new way to study the interaction between endophytic antinomyces and their host plants.
引文
Andrade L.F.,De Souza G.L.,Nietsche S.,Xavier A.A.,Costa M.R.,Cardoso A.M.,Pereira M.C.,and Pereira D.F.,2014,Analysis of the abilities of endophytic bacteria associated with banana tree roots to promote plant growth,Journal of Microbiology,52(1):27-34
Bertrand A.,Prvost D.,Bigras F.J.,and Castonguay Y.,2007,Elevated atmospheric CO2and strain of rhizobium alter freezing tolerance and cold-induced molecular changes in alfalfa(Medicago sativa),Annals of Botany,99(2):275-284
Chebotar V.K.,Malfanova N.V.,Shcherbakov A.V.,Ahtemova G.A.,Borisov A.Y.,Lugtenberg B.,and Tihonovich I.A.,2015,Endophytic bacteria in microbial preparations that improve plant development(review),Prikl Biokhim Mikrobiol,51(3):283-289
Zheng J.S.,and Lv P.,2003,PCR technique and its practical methods,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),1(3):381-389(郑景生,吕蓓,2003,PCR技术及实用方法,分子植物育种,1(3):381-389)
Geddes B.A.,Ryu M.H.,Mus F.,Garcia Costas A.,Peters J.W.,Voigt C.A.,and Poole P.,2015,Use of plant colonizing bacteria as chassis for transfer of N-2-fixation to cereals,Curr Opin Biotechnol,32:216-222
Ghodhbane-Gtari F.,Nouioui I.,Salem K.,Ktari A.,MonteroCalasanz Mdel C.,Tisa LS.,Klenk HP.,and Gtari M.,2014,Nocardia casuarinae sp.nov.,an actinobacterial endophyte isolated from root nodules of Casuarina glauca,Antonie Van Leeuwenhoek,105(6):1099-1106
Hallmann J.,Quadt Hallmann A.,Mahaffee W.F.,and Kloepper J.W.,1997,Bacterial endophytes in agricultural crops,Canadian Journal of Microbiology,43(10):895-914
Kothari S.K.,Marschner H.,and George E.,2010,Effect of VAmycorrhizal fungi and rhizosphere microorganisms on root and shoot morphology growth and water relations in maize,New Phytologist,116(2):303-311
Lee Y.C.,Johnson J.M.,Chien C.T.,Sun C.,Cai D.,Lou B.,Oelmller R.,and Yeh KW.,2011,Growth promotion of chinese cabbage and arabidopsis by Piriformospora indica is not stimulated by mycelium-synthesized auxin,Mol Plant Microbe Interact,24(4):421-431
Liao H.,Yuan S.,Yang Y.,Liu X.,Xu T.,Hu X.,Zeng X.,Zhang X.,Liu Y.,and Zhu Y.,2015,Screening and indentification of an endophytic streptomyces to antagonize rice blast,Journal of Hunan University Nutural Science,42:80-87
Liao H.,Tang R.,Zhang X.,Luan S.,and Yu F.,2017,FERONI-A receptor kinase at the crossroad of hormone signaling and stress responses,Plant Cell Physiol,58(7):1143-1150
Masachis S.,Segorbe D.,TurrD.,Leon-Ruiz M.,Frst U.,El Ghalid M.,Leonard G.,L佼pez-Berges M.S.,Richards T.A.,Felix G.,and Di Pietro A.,2016,A fungal pathogen secretes plant alkalinizing peptides to increase infection,Nat Microbiol,1(6):16043
Palaniyandi S.A.,Yang S.H.,Zhang L.X.,and Suh J.W.,2013,Effects of actinobacteria on plant disease suppression and growth promotion,Appl.Microbiol.Biotechnol.97(22):9621-9636
Passari A.K.,Chandr P.,Zothanpuia.,Mishra V.K.,Leo V.V.,Gupta V.K.,Kumar B.,and Singh B.P.,2016,Detection of biosynthetic gene and phytohormone production by endophytic actinobacteria associated with Solanum lycopersicum and their plant growth-promoting effect,Res Microbiol.,167(8):692-705
Perrine-Walker F.M.,Gartner E.,Hocart C.H.,Becker A.,and Rolfe B.G.,2007,Rhizobium-initiated rice growth inhibition caused by nitric oxide accumulation,Mol.Plant Microbe Interact,20(3):283-292
Pohjanen J.,Koskimaki J.J.,Sutela S.,Ardanov P.,Suorsa M.,Niemi K.,Sarjala T.,Hggman H.,and PirttilA.M.,2014,Interaction with ectomycorrhizal fungi and endophytic Methylobacterium affects nutrient uptake and growth of pine seedlings in vitro,Tree Physiol,34(9):993-1005
Rashada E.M.,Fathya H.M.,El-Zayata A.S.,and Eighonaimy A.M.,2015,Isolation and characterization of multifunctional Streptomyces species with antimicrobial,nematicidal and phytohormone activities frommarine environments in Egypt,Microbiol.Res.,175:34-47
Rani Debi B.,Taketa S.,and Ichii M.,2005,Cytokinin inhibits lateral root initiation but stimulates lateral root elongation in rice(Oryza sativa),J.Plant Physiol.,162(5):507-515
Shi C.L.,Park H.B.,Lee J.S.,Ryu S.,and Ryu C.M.,2010,Inhi bition of primary roots and stimulation of lateral root development in Arabidopsis thaliana by the rhizobacterium Serratia marcescens 90-166 is through both auxin-dependent and independent signaling pathways,Molecules and Cell,29:251-258
Sun J.,Kelemen G.H.,Fernandezabalos J.M.,and Bibb M.J.,1999,Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2),Microbiology,145(9):2221-2227
Tkacz A.,and Poole P.,2015,Role of root microbiota in plant productivity,J.Exp.Bot.,66(8):2167-2175
Thynne E.,Saur I.M.L.,Simbaqueba J.,Ogilvie H.A.,Gonzalez-Cendales Y.,Mead O.,Taranto A.,Catanzariti A.M.,Mc Donald M.C.,Schwessinger B.,Jones D.A.,Rathjen J.P.,and Solomon P.S.,2017,Fungal phytopathogens encode functional homologues of plant rapid alkalinization factor(RALF)peptides,Mol Plant Pathol,18(6):811-824
Verma V.C.,Singh S.K.,and Prakash S.,2011,Bio-control and plant growth promotion potential of siderophore producing endophytic Streptomyces from Azadirachta indica A.Juss,J.Basic Microbiol,51(5):550-556
Wang J.,Zhang Y.,Li Y.,Wang X.,Nan W.,Hu Y.,Zhang H.,Zhao C.,Wang F.,Li P.,Shi H.,and Bi Y.,2015,Endophytic microbes Bacillus sp.LZR216-regulated root development is dependent on polar auxin transport in Arabidopsis seedlings,Plant Cell Rep.,34(6):1075-1087
Zhou Y.,Zhang C.,Lin J.,Yang Y.,Peng Y.,Tang D.,Zhao X.,Zhu Y.,and Liu X.,2015,Over-expression of a glutamate dehydrogenase gene,Mg GDH,from Magnaporthe grisea confers tolerance to dehydration stress in transgenic rice,Planta,241(3):727-740
Bertrand A.,Prvost D.,Bigras F.J.,and Castonguay Y.,2007,Elevated atmospheric CO2and strain of rhizobium alter freezing tolerance and cold-induced molecular changes in alfalfa(Medicago sativa),Annals of Botany,99(2):275-284
Chebotar V.K.,Malfanova N.V.,Shcherbakov A.V.,Ahtemova G.A.,Borisov A.Y.,Lugtenberg B.,and Tihonovich I.A.,2015,Endophytic bacteria in microbial preparations that improve plant development(review),Prikl Biokhim Mikrobiol,51(3):283-289
Zheng J.S.,and Lv P.,2003,PCR technique and its practical methods,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),1(3):381-389(郑景生,吕蓓,2003,PCR技术及实用方法,分子植物育种,1(3):381-389)
Geddes B.A.,Ryu M.H.,Mus F.,Garcia Costas A.,Peters J.W.,Voigt C.A.,and Poole P.,2015,Use of plant colonizing bacteria as chassis for transfer of N-2-fixation to cereals,Curr Opin Biotechnol,32:216-222
Ghodhbane-Gtari F.,Nouioui I.,Salem K.,Ktari A.,MonteroCalasanz Mdel C.,Tisa LS.,Klenk HP.,and Gtari M.,2014,Nocardia casuarinae sp.nov.,an actinobacterial endophyte isolated from root nodules of Casuarina glauca,Antonie Van Leeuwenhoek,105(6):1099-1106
Hallmann J.,Quadt Hallmann A.,Mahaffee W.F.,and Kloepper J.W.,1997,Bacterial endophytes in agricultural crops,Canadian Journal of Microbiology,43(10):895-914
Kothari S.K.,Marschner H.,and George E.,2010,Effect of VAmycorrhizal fungi and rhizosphere microorganisms on root and shoot morphology growth and water relations in maize,New Phytologist,116(2):303-311
Lee Y.C.,Johnson J.M.,Chien C.T.,Sun C.,Cai D.,Lou B.,Oelmller R.,and Yeh KW.,2011,Growth promotion of chinese cabbage and arabidopsis by Piriformospora indica is not stimulated by mycelium-synthesized auxin,Mol Plant Microbe Interact,24(4):421-431
Liao H.,Yuan S.,Yang Y.,Liu X.,Xu T.,Hu X.,Zeng X.,Zhang X.,Liu Y.,and Zhu Y.,2015,Screening and indentification of an endophytic streptomyces to antagonize rice blast,Journal of Hunan University Nutural Science,42:80-87
Liao H.,Tang R.,Zhang X.,Luan S.,and Yu F.,2017,FERONI-A receptor kinase at the crossroad of hormone signaling and stress responses,Plant Cell Physiol,58(7):1143-1150
Masachis S.,Segorbe D.,TurrD.,Leon-Ruiz M.,Frst U.,El Ghalid M.,Leonard G.,L佼pez-Berges M.S.,Richards T.A.,Felix G.,and Di Pietro A.,2016,A fungal pathogen secretes plant alkalinizing peptides to increase infection,Nat Microbiol,1(6):16043
Palaniyandi S.A.,Yang S.H.,Zhang L.X.,and Suh J.W.,2013,Effects of actinobacteria on plant disease suppression and growth promotion,Appl.Microbiol.Biotechnol.97(22):9621-9636
Passari A.K.,Chandr P.,Zothanpuia.,Mishra V.K.,Leo V.V.,Gupta V.K.,Kumar B.,and Singh B.P.,2016,Detection of biosynthetic gene and phytohormone production by endophytic actinobacteria associated with Solanum lycopersicum and their plant growth-promoting effect,Res Microbiol.,167(8):692-705
Perrine-Walker F.M.,Gartner E.,Hocart C.H.,Becker A.,and Rolfe B.G.,2007,Rhizobium-initiated rice growth inhibition caused by nitric oxide accumulation,Mol.Plant Microbe Interact,20(3):283-292
Pohjanen J.,Koskimaki J.J.,Sutela S.,Ardanov P.,Suorsa M.,Niemi K.,Sarjala T.,Hggman H.,and PirttilA.M.,2014,Interaction with ectomycorrhizal fungi and endophytic Methylobacterium affects nutrient uptake and growth of pine seedlings in vitro,Tree Physiol,34(9):993-1005
Rashada E.M.,Fathya H.M.,El-Zayata A.S.,and Eighonaimy A.M.,2015,Isolation and characterization of multifunctional Streptomyces species with antimicrobial,nematicidal and phytohormone activities frommarine environments in Egypt,Microbiol.Res.,175:34-47
Rani Debi B.,Taketa S.,and Ichii M.,2005,Cytokinin inhibits lateral root initiation but stimulates lateral root elongation in rice(Oryza sativa),J.Plant Physiol.,162(5):507-515
Shi C.L.,Park H.B.,Lee J.S.,Ryu S.,and Ryu C.M.,2010,Inhi bition of primary roots and stimulation of lateral root development in Arabidopsis thaliana by the rhizobacterium Serratia marcescens 90-166 is through both auxin-dependent and independent signaling pathways,Molecules and Cell,29:251-258
Sun J.,Kelemen G.H.,Fernandezabalos J.M.,and Bibb M.J.,1999,Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2),Microbiology,145(9):2221-2227
Tkacz A.,and Poole P.,2015,Role of root microbiota in plant productivity,J.Exp.Bot.,66(8):2167-2175
Thynne E.,Saur I.M.L.,Simbaqueba J.,Ogilvie H.A.,Gonzalez-Cendales Y.,Mead O.,Taranto A.,Catanzariti A.M.,Mc Donald M.C.,Schwessinger B.,Jones D.A.,Rathjen J.P.,and Solomon P.S.,2017,Fungal phytopathogens encode functional homologues of plant rapid alkalinization factor(RALF)peptides,Mol Plant Pathol,18(6):811-824
Verma V.C.,Singh S.K.,and Prakash S.,2011,Bio-control and plant growth promotion potential of siderophore producing endophytic Streptomyces from Azadirachta indica A.Juss,J.Basic Microbiol,51(5):550-556
Wang J.,Zhang Y.,Li Y.,Wang X.,Nan W.,Hu Y.,Zhang H.,Zhao C.,Wang F.,Li P.,Shi H.,and Bi Y.,2015,Endophytic microbes Bacillus sp.LZR216-regulated root development is dependent on polar auxin transport in Arabidopsis seedlings,Plant Cell Rep.,34(6):1075-1087
Zhou Y.,Zhang C.,Lin J.,Yang Y.,Peng Y.,Tang D.,Zhao X.,Zhu Y.,and Liu X.,2015,Over-expression of a glutamate dehydrogenase gene,Mg GDH,from Magnaporthe grisea confers tolerance to dehydration stress in transgenic rice,Planta,241(3):727-740