高效液相色谱-四极杆飞行时间质谱联用仪测定小鼠钩吻素子中毒模型的研究
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摘要
目的通过动物急性钩吻素子染毒实验,建立一种测定小鼠钩吻素子的高效液相色谱-四极杆飞行时间质谱联用方法。方法小鼠经钩吻浸泡药酒染毒后取材,其血液、胃液、尿液通过1%甲酸乙腈沉淀蛋白后,进行上机分析。采用5 mmol/L乙酸铵-0.1%甲酸(A)和乙腈(B)作为流动相进行梯度洗脱,质谱正离子化信号采用母离子模式和子离子模式对钩吻素子进行监测。结果该实验结果显示1 h取材的动物样本,经过分析,胃液与尿液的差异性更大,且胃内容物钩吻素子含量较高。结论应急采样中,优先提供胃液物进行检验,其次到尿液。
Objective Through the animal acute leucin poisoning experiment, to establish a method for the detection of koumine in animals by high performance liquid chromatography-quadrupole time of flight mass spectrometer(HPLC-QTOF-MS). Methods After mice were poisoned with medicinal liquor, the protein in blood,gastric juice and urine of the mice were precipitated through 1% acetonitrile, and then analyzed by HPLC-QTOF-MS.Ammonium acetate-0.1% formic acid(A) and acetonitrile(B) were used as mobile phases for gradient elution.Koumine were monitored by mass spectrometry electrospray ionization(positive) using the mother ion model and daughter ion model. Results The results showed that the difference between gastric juice and urine was greater, and the koumine content of gastric contents was higher. Conclusion In emergency sampling, the first priority is to provide gastric juice for testing, followed by urine.
Objective Through the animal acute leucin poisoning experiment, to establish a method for the detection of koumine in animals by high performance liquid chromatography-quadrupole time of flight mass spectrometer(HPLC-QTOF-MS). Methods After mice were poisoned with medicinal liquor, the protein in blood,gastric juice and urine of the mice were precipitated through 1% acetonitrile, and then analyzed by HPLC-QTOF-MS.Ammonium acetate-0.1% formic acid(A) and acetonitrile(B) were used as mobile phases for gradient elution.Koumine were monitored by mass spectrometry electrospray ionization(positive) using the mother ion model and daughter ion model. Results The results showed that the difference between gastric juice and urine was greater, and the koumine content of gastric contents was higher. Conclusion In emergency sampling, the first priority is to provide gastric juice for testing, followed by urine.
引文
[1]许豪然,廖华军,王河山,等.钩吻的研究进展[J].中国民族民间医,2016,25(10):37-38.Xu HR,Liao HJ,Wang HS,et al.Advances in the study of Gukisheng[J].Chin Folk Med,2016,25(10):37-38.
[2]张志阳,伍勇,孙志良.钩吻药效学的研究进展[J].中国兽医杂志,2018,54(4):57-60.Zhang ZY,Wu Y,Sun ZL.Advances in pharmacodynamics of Gelsemia hookosa[J].Chin J Vet Med,2018,54(4):57-60.
[3]穆琼,于洪婕,韦卫琴,等.断肠草中毒临床救治体会[J].贵阳医学院学报,2012,37(2):206-210.Mu Q,Yu HJ,Wei WQ,et al.Experience in clinical treatment of enterotoxicosis[J].J Guiyang Med Coll,2012,37(2):206-210.
[4]黄科勇,苏泽球.钩吻中毒24例症状分析[J].刑事技术,1980,(3):12.Huang KY,Su ZQ.Symptom analysis of 24 cases of hook poisoning[J].Crimin Technol,1980,(3):12.
[5]冯时茵,栗建明,侯惠婵,等.钩吻生物碱成分的薄层色谱研究[J].中药新药与临床药理,2013,24(1):77-80.Feng SY,Li JM,Hou HC,et al.TLC study on alkaloids Gelsemia hookosa[J].New Chin Med Clin Pharm,2013,24(1):77-80.
[6]裴茂清,陈鸿义.钩吻生物碱的气相色谱分析方法[J].广东公安科技,1999,(1):42-44.Pei MQ,Chen HY.Gas chromatographic analysis of alkaloids from Gelsemia hookosa[J].Guangdong Publ Secur Sci Technol,1999,(1):42-44.
[7]丘宏强,程昱,刘茂柏,等.人血液中钩吻素甲和钩吻素子HPLC-UV测定[J].中草药,2016,47(13):2324-2327.Qiu HQ,Cheng Y,Liu MB,et al.Determination of gelatin A and gelatin Zi in human plasma by HPLC-UV[J].Chin Tradit Herb Drug,2016,47(13):2324-2327.
[8]蔡玉刚,张帆.气相色谱-质谱联用仪测定生物检材中钩吻生物碱[J].低碳世界,2016,(25):267-268.Cai YG,Zhang F.Gas chromatography-mass spectrometry for the determination of alkaloids in biological samples[J].Low Carbon World,2016,(25):267-268.
[9]陆亦在.断肠草中毒的GC-MS-MS检测方法[J].医学检验与临床,2013,(24):84-86.Lu YZ.GC-MS method for detection of enterotoxicity[J].Med Lab Clin,2013,(24):84-86.
[10]李雪,皮子凤,邢俊鹏,等.超高效液相色谱-质谱结合主成分分析方法研究制川乌配伍前后的肠内菌代谢差异[J].分析化学,2014,42(11):1646-1650.Li X,Pi ZF,Xing JP,et al.Ultra-high performance liquid chromatography-mass spectrometry combined with principal component analysis was used to study the metabolic differences of intestinal bacteria before and after compatibility of Radix Aconiti Preparata[J].Anal Chem,2014,42(11):1646-1650.
[11]曾茂茂,李洋,何志勇,等.液相色谱-质谱联用法结合主成分分析考察食品中前体物质对杂环胺生成的影响[J].分析化学,2014,42(1):71-76.Zeng MM,Li Y,He ZY,et al.The effect of precursors in food on the formation of heterocyclic amines was investigated by LC-MS combined with principal component analysis[J].Anal Chem,2014,42(1):71-76.
[12]Huang JT,Zhou Z,Gao ZH,et al.Aerodynamic multiobjective integrated optimization based on principal component analysis[J].Chin J Aeronaut,2014,35(9):2461-2471.
[13]Lv HB,Qian M,Li CH,et al.The principa component analysis on yielding and agronomic traits of hybrid rice of Liangyou 21111[J].Agric Sci Technol,2017,18(3):483-486.
[14]聂许妹,麦伟丽,黄翠连.断肠草中毒的院前急救与护理[J].基层医学论坛,2014,18(21):2876-2877.Nie XM,Mai WL,Huang CL.Pre-hospital first aid and nursing for intestinal grass poisoning[J].Prim Med Forum,2014,18(21):2876-2877.
[15]温金星.断肠草中毒18例临床分析[J].基层医学论坛,2013,17(28):3764-3765.Wen JX.Clinical analysis of 18 cases of enterotoxicity[J].Prim Med Forum,2013,17(28):3764-3765.
[16]钟延旭,梁玉裕,刘展华,等.一起农民工误食断肠草泡制酒引起中毒死亡事件调查分析[J].应用预防医学,2016,22(2):141-142.Zhong YX,Liang YY,Liu ZH,et al.Investigation and analysis of a fatal poisoning event caused by peasant workers'miseating of broken intestine straw to make wine[J].Appl Prev Med,2016,22(2):141-142.
[2]张志阳,伍勇,孙志良.钩吻药效学的研究进展[J].中国兽医杂志,2018,54(4):57-60.Zhang ZY,Wu Y,Sun ZL.Advances in pharmacodynamics of Gelsemia hookosa[J].Chin J Vet Med,2018,54(4):57-60.
[3]穆琼,于洪婕,韦卫琴,等.断肠草中毒临床救治体会[J].贵阳医学院学报,2012,37(2):206-210.Mu Q,Yu HJ,Wei WQ,et al.Experience in clinical treatment of enterotoxicosis[J].J Guiyang Med Coll,2012,37(2):206-210.
[4]黄科勇,苏泽球.钩吻中毒24例症状分析[J].刑事技术,1980,(3):12.Huang KY,Su ZQ.Symptom analysis of 24 cases of hook poisoning[J].Crimin Technol,1980,(3):12.
[5]冯时茵,栗建明,侯惠婵,等.钩吻生物碱成分的薄层色谱研究[J].中药新药与临床药理,2013,24(1):77-80.Feng SY,Li JM,Hou HC,et al.TLC study on alkaloids Gelsemia hookosa[J].New Chin Med Clin Pharm,2013,24(1):77-80.
[6]裴茂清,陈鸿义.钩吻生物碱的气相色谱分析方法[J].广东公安科技,1999,(1):42-44.Pei MQ,Chen HY.Gas chromatographic analysis of alkaloids from Gelsemia hookosa[J].Guangdong Publ Secur Sci Technol,1999,(1):42-44.
[7]丘宏强,程昱,刘茂柏,等.人血液中钩吻素甲和钩吻素子HPLC-UV测定[J].中草药,2016,47(13):2324-2327.Qiu HQ,Cheng Y,Liu MB,et al.Determination of gelatin A and gelatin Zi in human plasma by HPLC-UV[J].Chin Tradit Herb Drug,2016,47(13):2324-2327.
[8]蔡玉刚,张帆.气相色谱-质谱联用仪测定生物检材中钩吻生物碱[J].低碳世界,2016,(25):267-268.Cai YG,Zhang F.Gas chromatography-mass spectrometry for the determination of alkaloids in biological samples[J].Low Carbon World,2016,(25):267-268.
[9]陆亦在.断肠草中毒的GC-MS-MS检测方法[J].医学检验与临床,2013,(24):84-86.Lu YZ.GC-MS method for detection of enterotoxicity[J].Med Lab Clin,2013,(24):84-86.
[10]李雪,皮子凤,邢俊鹏,等.超高效液相色谱-质谱结合主成分分析方法研究制川乌配伍前后的肠内菌代谢差异[J].分析化学,2014,42(11):1646-1650.Li X,Pi ZF,Xing JP,et al.Ultra-high performance liquid chromatography-mass spectrometry combined with principal component analysis was used to study the metabolic differences of intestinal bacteria before and after compatibility of Radix Aconiti Preparata[J].Anal Chem,2014,42(11):1646-1650.
[11]曾茂茂,李洋,何志勇,等.液相色谱-质谱联用法结合主成分分析考察食品中前体物质对杂环胺生成的影响[J].分析化学,2014,42(1):71-76.Zeng MM,Li Y,He ZY,et al.The effect of precursors in food on the formation of heterocyclic amines was investigated by LC-MS combined with principal component analysis[J].Anal Chem,2014,42(1):71-76.
[12]Huang JT,Zhou Z,Gao ZH,et al.Aerodynamic multiobjective integrated optimization based on principal component analysis[J].Chin J Aeronaut,2014,35(9):2461-2471.
[13]Lv HB,Qian M,Li CH,et al.The principa component analysis on yielding and agronomic traits of hybrid rice of Liangyou 21111[J].Agric Sci Technol,2017,18(3):483-486.
[14]聂许妹,麦伟丽,黄翠连.断肠草中毒的院前急救与护理[J].基层医学论坛,2014,18(21):2876-2877.Nie XM,Mai WL,Huang CL.Pre-hospital first aid and nursing for intestinal grass poisoning[J].Prim Med Forum,2014,18(21):2876-2877.
[15]温金星.断肠草中毒18例临床分析[J].基层医学论坛,2013,17(28):3764-3765.Wen JX.Clinical analysis of 18 cases of enterotoxicity[J].Prim Med Forum,2013,17(28):3764-3765.
[16]钟延旭,梁玉裕,刘展华,等.一起农民工误食断肠草泡制酒引起中毒死亡事件调查分析[J].应用预防医学,2016,22(2):141-142.Zhong YX,Liang YY,Liu ZH,et al.Investigation and analysis of a fatal poisoning event caused by peasant workers'miseating of broken intestine straw to make wine[J].Appl Prev Med,2016,22(2):141-142.