RP-HPLC法同时测定平消片中3种成分
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摘要
目的建立RP-HPLC法同时测定平消片(枳壳、郁金、仙鹤草等)中3种成分的含有量。方法该药物甲醇提取液的分析采用Thermo C18色谱柱(5μm,4. 6 mm×250 mm);流动相乙腈-0. 01%磷酸,梯度洗脱;体积流量1. 0 mL/min;柱温30℃;检测波长283 nm。结果柚皮苷、橙皮苷、新橙皮苷分别在45. 0～734. 4μg/mL (r=0. 999 7)、2. 71～43. 36μg/mL (r=0. 999 7)、25. 02～400. 4μg/mL (r=0. 999 9)范围内线性关系良好,平均加样回收率分别为96. 33%(RSD=1. 8%)、97. 63%(RSD=1. 8%)、96. 79%(RSD=1. 8%)。结论该方法简便可靠,重复性好,可用于平消片的质量控制。
AIM To establish an RP-HPLC method for the simultaneous content determination of three constituents in Pingxiao Tablets(Aurantii Fructus,Curcumae Radix,Agrimoniae Herba,etc.). METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Thermo C18 column(5 μm,4. 6 mm ×250 mm),with the mobile phase comprising of acetonitrile-0. 01% phosphrous acid flowing at 1. 0 mL/min in a gradient elution manner,and the detection wavelength was set at 283 nm. RESULTS Naringin,hesperidin and neohespridin showed good linear relationships within the ranges of 45-734. 4 μg/mL(r = 0. 999 7),2. 71-43. 36 μg/mL(r = 0. 999 7) and 25. 02-400. 4 μg/mL(r = 0. 999 9),whose average recoveries were 96. 33%(RSD = 1. 8%),97. 63%(RSD = 1. 8%) and 96. 79%(RSD = 1. 8%),respectively. CONCLUSION This simple,reliable and reproducible method can be used for the quality control of Pingxiao Tablets.
AIM To establish an RP-HPLC method for the simultaneous content determination of three constituents in Pingxiao Tablets(Aurantii Fructus,Curcumae Radix,Agrimoniae Herba,etc.). METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Thermo C18 column(5 μm,4. 6 mm ×250 mm),with the mobile phase comprising of acetonitrile-0. 01% phosphrous acid flowing at 1. 0 mL/min in a gradient elution manner,and the detection wavelength was set at 283 nm. RESULTS Naringin,hesperidin and neohespridin showed good linear relationships within the ranges of 45-734. 4 μg/mL(r = 0. 999 7),2. 71-43. 36 μg/mL(r = 0. 999 7) and 25. 02-400. 4 μg/mL(r = 0. 999 9),whose average recoveries were 96. 33%(RSD = 1. 8%),97. 63%(RSD = 1. 8%) and 96. 79%(RSD = 1. 8%),respectively. CONCLUSION This simple,reliable and reproducible method can be used for the quality control of Pingxiao Tablets.
引文
[1]贾堃.中医癌瘤学[M].西安:陕西科学技术出版社,1996:64-69.
[2]高学忠,董树枫,周瑛.平消片为主组合方案治疗乳腺增生症临床分析[J].浙江中西医结合杂志,2004,14(2):87-88.
[3]王世兰.平消片治疗乳腺增生病108例[J].吉林中医药,2003,23(5):25.
[4]国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:246.
[5]舒尊鹏,胡书法,翟亚东,等.中药枳壳化学成分及药理作用研究[J].科技创新与应用,2012(17):8-9.
[6]章斌,金剑,金芝贵,等.枳壳的药理作用与临床应用进展[J].医药导报,2013,32(11):1462-1464.
[7]季忆,陈建真,陈建明.枳壳黄酮类成分的研究进展[J].中国中医药信息杂志,2010,17(11):105-107.
[8]郭增喜,李文庭,李兆奎.不同产地枳壳中柚皮苷和新橙皮苷的测定[J].中草药,2012,43(7):1347-1348.
[9]董玄,高文远,李凤阁,等. RP-HPLC法测定枳壳药材中柚皮苷、橙皮苷、新橙皮苷[J].中草药,2009,40(S1):283-284.
[10]国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:772-773.
[11]张军龙,濮存海,贾晓波,等.一种治疗乳腺增生的中药组合物及其制备方法和应用:中国,CN106039210A[P].2016-10-26.
[12]张军龙,濮存海,贾晓波,等.一种治疗乳腺增生的中药提取物及其制备方法和应用:中国,CN106039209A[P].2016-10-26.
[2]高学忠,董树枫,周瑛.平消片为主组合方案治疗乳腺增生症临床分析[J].浙江中西医结合杂志,2004,14(2):87-88.
[3]王世兰.平消片治疗乳腺增生病108例[J].吉林中医药,2003,23(5):25.
[4]国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:246.
[5]舒尊鹏,胡书法,翟亚东,等.中药枳壳化学成分及药理作用研究[J].科技创新与应用,2012(17):8-9.
[6]章斌,金剑,金芝贵,等.枳壳的药理作用与临床应用进展[J].医药导报,2013,32(11):1462-1464.
[7]季忆,陈建真,陈建明.枳壳黄酮类成分的研究进展[J].中国中医药信息杂志,2010,17(11):105-107.
[8]郭增喜,李文庭,李兆奎.不同产地枳壳中柚皮苷和新橙皮苷的测定[J].中草药,2012,43(7):1347-1348.
[9]董玄,高文远,李凤阁,等. RP-HPLC法测定枳壳药材中柚皮苷、橙皮苷、新橙皮苷[J].中草药,2009,40(S1):283-284.
[10]国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:772-773.
[11]张军龙,濮存海,贾晓波,等.一种治疗乳腺增生的中药组合物及其制备方法和应用:中国,CN106039210A[P].2016-10-26.
[12]张军龙,濮存海,贾晓波,等.一种治疗乳腺增生的中药提取物及其制备方法和应用:中国,CN106039209A[P].2016-10-26.