美洲大蠊中蛋白质和糖胺聚糖双指标的提取工艺研究
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摘要
目的:研究美洲大蠊中总蛋白质和糖胺聚糖的含量及提取工艺。方法:美洲大蠊通过水提酶解法提取,设计正交实验从料液比、超声时间和提取温度三方面考察,总蛋白质通过改良lowry法测定,糖胺聚糖通过阿利新蓝染色法测定,以蛋白质和糖胺聚糖的提取率为指标,筛选最佳提取工艺。结果:美洲大蠊中蛋白质及糖胺聚糖的最佳提取条件为:料液比1∶30、超声时间70 min及温度40℃,其中总蛋白的提取率为27.79%,糖胺聚糖的提取率2.31%。结论:通过美洲大蠊的双指标优化提取工艺,有利于发挥多成分功效,提高其药理研究效果。
Objective:To study the content and extraction technology of total protein and glycosaminoglycan in Periplaneta americana.Method: Periplaneta Americana was extracted by enzymatic hydrolysis with water extraction.Orthogonal experiments were designed to investigate the ratio of material to liquid,ultrasonic time and extraction temperature.Total protein was determined by modified Lowry method,and glycosaminoglycan was determined by alisine blue staining.The extraction rate of protein and glycosaminoglycan was used as index to screen the best extraction process.Result: The optimum extraction conditions of protein and glycosaminoglycan from Periplaneta Americana were as follows: ratio of material to liquid 1∶30,ultrasonic time 70 min and temperature 40 C.The extraction rate of total protein and glycosaminoglycan was 27.79% and 2.31%.Conclusion: To optimize the extraction process of Periplaneta Americana with two indicators is conducive to giving full play to the efficacy of multi-components and improving the pharmacological research effect of Periplaneta Americana.
Objective:To study the content and extraction technology of total protein and glycosaminoglycan in Periplaneta americana.Method: Periplaneta Americana was extracted by enzymatic hydrolysis with water extraction.Orthogonal experiments were designed to investigate the ratio of material to liquid,ultrasonic time and extraction temperature.Total protein was determined by modified Lowry method,and glycosaminoglycan was determined by alisine blue staining.The extraction rate of protein and glycosaminoglycan was used as index to screen the best extraction process.Result: The optimum extraction conditions of protein and glycosaminoglycan from Periplaneta Americana were as follows: ratio of material to liquid 1∶30,ultrasonic time 70 min and temperature 40 C.The extraction rate of total protein and glycosaminoglycan was 27.79% and 2.31%.Conclusion: To optimize the extraction process of Periplaneta Americana with two indicators is conducive to giving full play to the efficacy of multi-components and improving the pharmacological research effect of Periplaneta Americana.
引文
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[12]史磊,刘春雨,张盼盼,等.正交实验法优选发酵蜈蚣粉中水溶性总蛋白提取工艺及其抗肿瘤效果[J].山东中医杂志,2017,36(8):710-713.
[2]林建原,王杰,张盼盼.蛏子糖胺聚糖的提取及纯化[J].中国食品学报,2014,14(1):121-128.
[3]王彦多,刘春雨,吕世洁,等.正交实验法优化发酵蜈蚣粉中总黄酮提取工艺[J].食品工业科技,2017,38(7):183-186,192.
[4] 司金光,张涛,李凌宇,等.美洲大蠊的化学成分研究[J].中国药学杂志,2018,53(3):178-181.
[5]谭巧云,杨永寿,李开凤,等.美洲大蠊卵鞘中16种元素分析[J].大理大学学报,2016,1(4):22-25.
[6]袁东强,何钊,孙龙,等.美洲大蠊油脂的提取及性质分析[J].中国粮油学报,2016,31(3):84-90.
[7]焦方文,张盼盼.全蝎蛋白的超声提取工艺研究[J].山东中医杂志,2016,35(9):824-825.
[8]张鹭.昆虫表皮蛋白的研究进展及其应用前景[J].广东蚕业,2006,40(3):38-42.
[9]穆利霞,廖森泰,肖更生,等.昆虫蛋白的综合利用研究进展[J].现代食品科技,2011,27(12):1507-1512.
[10]潘怡欧.昆虫蛋白质提取工艺的比较研究[D].长春:吉林农业大学,2005.
[11]桑晓,韩海燕,王彦多,等.蜘蛛粉中糖胺聚糖提取工艺的优化[J].山东化工,2018,47(8):26-29.
[12]史磊,刘春雨,张盼盼,等.正交实验法优选发酵蜈蚣粉中水溶性总蛋白提取工艺及其抗肿瘤效果[J].山东中医杂志,2017,36(8):710-713.