植物根际促生菌(PGPR)对非宿主植物猫尾草和小黑麦生长的促生作用
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摘要
为了探究根际促生菌(PGPR)对非宿主植物[猫尾草(Uraria crinita)、小黑麦(×Triticale Wittmack)]生长的影响,本研究采用甘肃农业大学草业学院微生物实验室提供的10株具有固氮、溶磷、分泌激素和生防特性的PGPR菌株为供试菌株,筛选最佳菌种组合,并按一定比例混合制作微生物复合菌肥,在盆栽条件下,研究菌肥对非宿主植物小黑麦、猫尾草生长的影响。结果表明:1)通过试验筛选出的最佳菌种组合为NCRP2+PPRS3+G+ZKRP2;2)宿主植物菌肥(NF_1)处理对小黑麦地上生物量鲜重影响显著(P<0.05),施用非宿主植物菌肥(NF_2)后与施用NF_1相比,猫尾草茎粗增加33.74%,且差异显著(P<0.05);3)NF_2处理影响小黑麦根平均直径、根体积,与对照相比分别增加22.54%和125%,差异显著(P<0.05),NF2处理对根直径0.5~1.0和1.0~1.5 mm小黑麦根长影响显著(P<0.05)。PGPR菌肥对非宿主植物猫尾草和小黑麦生长具有明显的促生效果。
This study focused on several aspects of the different effects of compound microbial fertilizer containing Plant Growth-Promoting Rhizobacteria(PGPR)on the growth of non-host plants(Uraria crinita and ×Triticale Wittmack).Several excellent strains were screened from among 10 strains treated with PGPR provided by our laboratory(Gansu Agricultural University Microbiology Laboratory).A new fertilizer containing several typesof bacteria was prepared.To determine the effect of the bacterial fertilizer on the growth of the nonhost plants,potted testswere conducted.The results showed that:1)four excellent strains,NCRP2,PPRS3,G,and ZKRP2,were identified;2)theeffect of host plant bio-fertilizer(NF_1)on the fresh weight of aboveground triticale plants was significant(P<0.05).After applying non-host plant fertilizer(NF_2),compared with NF_1,the stem diameter of Timothy was significantly increased by 33.74%(P<0.05);3)NF_2 treatment increased the average root diameter and root volume of the triticales by 22.54% and 125%,respectively,compared with thecontrol CK(P<0.05).NF_2 treatment significantly affected root lengths of 0.5~1.0 mm triticales(P<0.05).The effect of NF_1 on the root lengths of 1.0~1.5 mm triticales was significant(P<0.05).Based on these results,we suggest that to prepare a new compound microbial fertilizer,the origin of the strain cannot be confined to the host plant rhizosphere,but rather other excellent PGPR strains should be considered.
This study focused on several aspects of the different effects of compound microbial fertilizer containing Plant Growth-Promoting Rhizobacteria(PGPR)on the growth of non-host plants(Uraria crinita and ×Triticale Wittmack).Several excellent strains were screened from among 10 strains treated with PGPR provided by our laboratory(Gansu Agricultural University Microbiology Laboratory).A new fertilizer containing several typesof bacteria was prepared.To determine the effect of the bacterial fertilizer on the growth of the nonhost plants,potted testswere conducted.The results showed that:1)four excellent strains,NCRP2,PPRS3,G,and ZKRP2,were identified;2)theeffect of host plant bio-fertilizer(NF_1)on the fresh weight of aboveground triticale plants was significant(P<0.05).After applying non-host plant fertilizer(NF_2),compared with NF_1,the stem diameter of Timothy was significantly increased by 33.74%(P<0.05);3)NF_2 treatment increased the average root diameter and root volume of the triticales by 22.54% and 125%,respectively,compared with thecontrol CK(P<0.05).NF_2 treatment significantly affected root lengths of 0.5~1.0 mm triticales(P<0.05).The effect of NF_1 on the root lengths of 1.0~1.5 mm triticales was significant(P<0.05).Based on these results,we suggest that to prepare a new compound microbial fertilizer,the origin of the strain cannot be confined to the host plant rhizosphere,but rather other excellent PGPR strains should be considered.
引文
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[18]赵宏魁,马真,张春辉,雷占兰,姚步青,周华坤.种植密度和施氮水平对燕麦生物量分配的影响.草业科学,2016,33(2):249-258.ZHAO H K,MA Z,ZHANG C H,LEI Z L,YAO B Q,ZHOU H K.The reproductive allocation of Avena sativa under different planting densities and nitrogen addition treatments.Pratacultural Science,2016,33(2):249-258.
[19]宋锐,林选栋,林丽果,宋浩然,刘慧霞.不同盐浓度下硅对高羊茅根系特征的影响.草业科学,2017,34(6):1188-1193.SONG R,LIN X D,LIN L G,SONG H R,LIU H X.Effect of silicon supply on root features of tall fescue in different salt concentrations.2017,34(6):1188-1193.
[20]秦士娇,李娟,王远宏,康芬芬,刘可,樊乐,常若葵.黄瓜PGPR菌株S160的鉴定及其对重金属铅的吸附研究.中国生物防治学报,2016,32(1):129-134.QING S J,LI J,WANG Y H,KANG F F,LIU K,FAN L,CHANG R K.Identification of PGPR strain S160on cucumber and its adsorption for lead ions.Chinese Journal of Biological Control,2016,32(1):129-134.
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[2]龙伟文,王平,冯新梅,胡正嘉,李阜棣.PGPR与AMF相互关系的研究进展.应用生态学报,2000,11(2):311-314.LONG W W,WANG P,FENG X M,HU Z J,LI G L.Research progress on PGPR/AMF interactions.Chinese Journal of Applied Ecology,2000,11(2):311-314.
[3]孟瑶,徐凤花,孟庆有,顾万荣.中国微生物肥料研究及应用进展.中国农学通报,2008,24(6):276-283.MENG Y,XU F H,MENG Q Y,GU W R.Current application status and prospect of microbiological fertilizer in China.Chinese Agricultural Science Bulletin,2008,24(6):276-283.
[4]韩华雯,姚拓,王国基,赵桂琴,张玉霞,马文文,马文彬.不同根际促生菌肥复合载体对燕麦产量的影响.草原与草坪,2013,33(4):39-44.HAN H W,YAO T,WANG G J,ZHAO G Q,ZHANG Y X,MA W W,MA W B.Effect of different bio-fertilixers on hay yield of oat.Grassland and Turf,2013,33(4):39-44.
[5]刘丹丹,李敏,刘润进.我国植物根围促生细菌研究进展.生态学杂志,2016,35(3):815-824.LIU D D,LI M,LIU R J.Recent advances in the study of plant growth-promoting rhizobacteria in China.Chinese Journal of Ecology,2016,35(3):815-824.
[6]胡江春,薛德林,马成新,王书锦.植物根际促生菌(PGPR)的研究与应用前景.应用生态学报,2004,15(10):1963-1966.HU J C,XUE D L,MA C X,WANG S J.Research advances in plant growth-promoting rhizobacteria and its application prospects.Chinese Journal of Applied Ecology,2004,15(10):1963-1966.
[7]陈晓斌,张炳欣,楼兵干,Ryder M H.运用生色基因标记黄瓜根围促生菌(PGPR)筛选菌株.微生物学报,2001(3):287-292.CHEN X B,ZHANG B X,LOU B G,RYDER M H.Introduction of the chromogenic gene to the plant growth-promoting rhizobacteria of cucumber.Acta Microbiologica Sinica,2001(3):287-292.
[8]KLOEPPER J W,RODRIGUEZ-UBANA G W,ZEHNDER B.Plant root bacterial interactions in biological control of soil borne diseases and potential extension to systemic and foliar diseases.Australasian Plant Pathology,1999.28(1):21-26.
[9]孙广正.微生物接种剂对油菜和西葫芦病害防治及其促生作用研究.兰州:甘肃农业大学硕士学位论文,2015.SUN G Z.Control effect of bio-inoculant to diseases on rapeseed and zucchini and its growth promoting capacity.Maste Thesis.Lanzhou:Gansu Agricultural University,2015.
[10]韩文星.PGPR菌肥研制及其对燕麦生长和品质影响的研究.兰州:甘肃农业大学硕士学位论文,2007.HAN W X.PGPR biofertilizers producing and effect on oat growth and quality development.Master Thesis.Lanzhou:Gansu Agricultural University,2007.
[11]康贻军,程洁,梅丽娟,殷士学.植物根际促生菌的筛选及鉴定.微生物学报,2010,50(7):853-861.KANG Y J,CHEN J,MEI L J,YING S X.Screening and identification of plant growth-promoting rhizobacteria.Acta Microbiologica Sinica,2010,50(7):853-861.
[12]刘青海,姚拓,马从,李显刚.6株溶磷菌和4株固氮菌混合培养条件的研究.草原与草坪,2011,31(6):1-6,13.LIU Q H,YAO T,MA C,LI X G.Cultural conditions for mixed culture of phosphate solubilizing and nitrogen-fixing bacteria.Grassland and Turf,2011,31(6):1-6,13.
[13]刘婷.高寒草甸优势植物根际促生菌资源评价及菌种鉴定.兰州:甘肃农业大学硕士学位论文,2016.LIU T.Evaluating and identifying of plant growth promoting rhizobacteria of dominant alpine meadows.Master Thesis.Lanzhou:Gansu Agricultural University,2016.
[14]任大明,张晓轩,董丹,刘伟成,刘德文.拮抗菌株Kc-t99的鉴定及其抑菌活性研究.生物技术通报,2011(4):153-157.REN D M,ZHANG X X,DONG D,LIU W C,LIU D W.Studies on taxonomy and microbiostatic activity of antagonistic strain Kc-t99.Biotechnology Bulletin,2011(4):153-157.
[15]张良,刘好宝,顾金刚,宋毓峰,董连红,苏玉龙,李世贵,雷强,伍仁军,殷英,屈健康,王勇.长柄木霉和泾阳链霉菌复配对烟苗生长及其抗病性的影响.应用生态学报,2013,24(10):2961-2969.ZHANG L,LIU H B,GU J G,SONG Y F,DONG L H,SU Y L,LI S G,LEI Q,WU R J,YING Y,QU J K,WANG Y.Effects of Trichoderma longbrachitum and Streptomyces jingyangensis combination on the growth and disease resistance of tobacco seedlings.Chinese Journal of Applied Ecology,2013,24(10):2961-2969.
[16]王永嘉,成慧,Eun J K,Nigel S,常生华,侯扶江.黄土高原高糖黑麦草的产量和品质预测.草业科学,2017,34(9):1863-1879.WANG Y J,CHENG H,EUN J K,NIGEL S,CHANG S H,HOU F J.Prediction of yield and quality of high sugar ryegrass inLoess plateau.Pratacultural Science,2017,34(9):1863-1879.
[17]李雪,田新会,杜文华.饲草型小黑麦苗期抗旱指标的筛选.草业科学,2017,34(3):539-546.LI X,TIAN X H,DU W H.Screening on the drought resistance index and conditions for Triticale Wittmack at the seedling stage.Pratacultural Science.2017,34(3):539-546.
[18]赵宏魁,马真,张春辉,雷占兰,姚步青,周华坤.种植密度和施氮水平对燕麦生物量分配的影响.草业科学,2016,33(2):249-258.ZHAO H K,MA Z,ZHANG C H,LEI Z L,YAO B Q,ZHOU H K.The reproductive allocation of Avena sativa under different planting densities and nitrogen addition treatments.Pratacultural Science,2016,33(2):249-258.
[19]宋锐,林选栋,林丽果,宋浩然,刘慧霞.不同盐浓度下硅对高羊茅根系特征的影响.草业科学,2017,34(6):1188-1193.SONG R,LIN X D,LIN L G,SONG H R,LIU H X.Effect of silicon supply on root features of tall fescue in different salt concentrations.2017,34(6):1188-1193.
[20]秦士娇,李娟,王远宏,康芬芬,刘可,樊乐,常若葵.黄瓜PGPR菌株S160的鉴定及其对重金属铅的吸附研究.中国生物防治学报,2016,32(1):129-134.QING S J,LI J,WANG Y H,KANG F F,LIU K,FAN L,CHANG R K.Identification of PGPR strain S160on cucumber and its adsorption for lead ions.Chinese Journal of Biological Control,2016,32(1):129-134.
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