6种药用植物的2个组织的内生细菌多样性初步分析
|
摘要
为了解白英、巴天酸模、葎草、泽漆、商陆、野老鹳草6种植物不同采集地点不同器官中内生细菌的群落分布及其规律,分别提取植物根和叶的总DNA,应用PCR-DGGE技术对6种植物3处地点的根叶共36个样本的细菌群落进行了研究,根据DGGE图谱,对它们的菌落多样性进行了分析。结果表明:不同植物的内生细菌群落在种类和含量上均有差异;同种植物不同器官的内生细菌在种类上无明显差异,含量上有差异;不同地点的同种植物内生菌相似性较高,差别不明显。可见生长环境以及植物不同部位对于内生菌群落分布具有一定的影响。
To understand the community distribution and regularity of endophytic bacteria in different organs of different plants in Solanum lyratumThunb,Rumexpatientia Linn,Humulus scandens(Lour.)Merr,EuphorbiahelioscopiaL,PhytolaccaacinosaRoxb,Geranium carolinianum L.Total DNA of plant roots and leaves were extracted.PCRDGGE technique was used to study the bacterial community of 36 samples from roots and leaves of 6plants.The colony diversity was analyzed according to DGGE map.Result:The endophytic bacterial communities of different plants were different in species and content.There were no significant differences in endophytic bacteria in different organs of the same plant,and the content was different.The similarity of endophytic bacteria of the same plant in different sites was higher.The difference is not obvious.Conclusion:The growth environment and different parts of plants have certain effects on the distribution of endophytic communities.
To understand the community distribution and regularity of endophytic bacteria in different organs of different plants in Solanum lyratumThunb,Rumexpatientia Linn,Humulus scandens(Lour.)Merr,EuphorbiahelioscopiaL,PhytolaccaacinosaRoxb,Geranium carolinianum L.Total DNA of plant roots and leaves were extracted.PCRDGGE technique was used to study the bacterial community of 36 samples from roots and leaves of 6plants.The colony diversity was analyzed according to DGGE map.Result:The endophytic bacterial communities of different plants were different in species and content.There were no significant differences in endophytic bacteria in different organs of the same plant,and the content was different.The similarity of endophytic bacteria of the same plant in different sites was higher.The difference is not obvious.Conclusion:The growth environment and different parts of plants have certain effects on the distribution of endophytic communities.
引文
[1]张冬静.药用植物内生真菌的分离及其抑菌活性筛选[D].长春:吉林大学,2015.
[2]胡萌.植物内生细菌研究进展[J].山东农业大学学报(自然科学版),2008,39(1):148~151.
[3]邓雪萍,杨博.药用植物内生菌及其产生的活性成分研究现状[J].安徽农学通报(上半月刊),2010,16(1):72~74.
[4]王莉衡.植物内生菌的研究进展[J].化学与生物工程,2011,28(3):5~7,18.
[5]张秀娟,马悦.白英的药理作用研究进展[J].亚太传统医药,2008,4(1):54~56.
[6]朱晶晶,王峥涛,张朝凤,等.酸模属植物中化学成分及其药理活性研究进展[J].中草药,2008(3):450~454.
[7]尹海波,王颖,郑太坤,等.中国葎草属植物的研究进展[J].辽宁中医学院学报,2001,3(1):60~61.
[8]杨莉,陈海霞,高文远.泽漆化学成分及其体外抗肿瘤活性研究[J].天然产物研究与开发,2008,20(4):575~577.
[9]李一飞,姚广涛.商陆药理作用及毒性研究进展[J].中国实验方剂学杂志,2011(13):248~251.
[10]李继阳.野老鹳草抗乙肝病毒作用及化学组分研究[D].上海:复旦大学,2008.
[11]王志伟,纪燕玲,陈永敢.植物内生菌研究及其科学意义[J].微生物学通报,2015,42(2):349~363.
[12]王文斗,段英俊,那冬晨.植物总DNA提取方法的改进[J].安徽农业科学,2013,41(24):9913,9923.
[13]黄雅丽,马嫚,张韧,等.DNA提取方法对桑树内生菌多样性研究的影响[J].广州中医药大学学报,2015,32(4):729~734.
[14]Jasalavich C A,Ostrofsky A,Jellison J.Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA[J].Appl Environ Microb,2000,66(11):4725.
[15]彭三妹,王博林,路京,等.浙贝母中内生菌的多样性及组成分析[J].浙江中医药大学学报,2014,38(9):1091~1097,1102.
[16]张珣,李燕,刘晓,等.红地球葡萄健康叶片内生细菌多样性分析[J].植物病理学报,2017,47(1):107~116.
[17]Muyzer G,Waal ECD,Uitterlinden AG.Profiling complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for16SrRNA[J].Applied and Environmental Microbiology,1993(59):695~700.
[18]马悦欣.变性梯度凝胶电泳(DGGE)在微生物生态学中的应用[J].生态学报,2003,23(8):1561~1569.
[19]洪完国,江颖,彭韶军,等.蕲春县蕲艾产业发展现状及对策[J].中南民族大学学报(自然科学版),2017(4):64~66.
[2]胡萌.植物内生细菌研究进展[J].山东农业大学学报(自然科学版),2008,39(1):148~151.
[3]邓雪萍,杨博.药用植物内生菌及其产生的活性成分研究现状[J].安徽农学通报(上半月刊),2010,16(1):72~74.
[4]王莉衡.植物内生菌的研究进展[J].化学与生物工程,2011,28(3):5~7,18.
[5]张秀娟,马悦.白英的药理作用研究进展[J].亚太传统医药,2008,4(1):54~56.
[6]朱晶晶,王峥涛,张朝凤,等.酸模属植物中化学成分及其药理活性研究进展[J].中草药,2008(3):450~454.
[7]尹海波,王颖,郑太坤,等.中国葎草属植物的研究进展[J].辽宁中医学院学报,2001,3(1):60~61.
[8]杨莉,陈海霞,高文远.泽漆化学成分及其体外抗肿瘤活性研究[J].天然产物研究与开发,2008,20(4):575~577.
[9]李一飞,姚广涛.商陆药理作用及毒性研究进展[J].中国实验方剂学杂志,2011(13):248~251.
[10]李继阳.野老鹳草抗乙肝病毒作用及化学组分研究[D].上海:复旦大学,2008.
[11]王志伟,纪燕玲,陈永敢.植物内生菌研究及其科学意义[J].微生物学通报,2015,42(2):349~363.
[12]王文斗,段英俊,那冬晨.植物总DNA提取方法的改进[J].安徽农业科学,2013,41(24):9913,9923.
[13]黄雅丽,马嫚,张韧,等.DNA提取方法对桑树内生菌多样性研究的影响[J].广州中医药大学学报,2015,32(4):729~734.
[14]Jasalavich C A,Ostrofsky A,Jellison J.Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA[J].Appl Environ Microb,2000,66(11):4725.
[15]彭三妹,王博林,路京,等.浙贝母中内生菌的多样性及组成分析[J].浙江中医药大学学报,2014,38(9):1091~1097,1102.
[16]张珣,李燕,刘晓,等.红地球葡萄健康叶片内生细菌多样性分析[J].植物病理学报,2017,47(1):107~116.
[17]Muyzer G,Waal ECD,Uitterlinden AG.Profiling complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for16SrRNA[J].Applied and Environmental Microbiology,1993(59):695~700.
[18]马悦欣.变性梯度凝胶电泳(DGGE)在微生物生态学中的应用[J].生态学报,2003,23(8):1561~1569.
[19]洪完国,江颖,彭韶军,等.蕲春县蕲艾产业发展现状及对策[J].中南民族大学学报(自然科学版),2017(4):64~66.