大豆种质资源对灰斑病抗性遗传变异研究
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摘要
本研究旨在为培育高抗灰斑病品种挖掘优良种质资源。以1164份大豆资源(国内915份、国外145份和野生104份)为试验材料,在人工接种大豆灰斑病病菌条件下鉴定材料抗病性。1164份供试材料灰斑病病情指数平均为52.2%,遗传变异系数为23.9%;64.9%的品种具有灰斑病抗性;抗性最高的是‘承豆6号’,病情指数为10%;国外品种资源群体对灰斑病抗性高于野生和国内的;国内13个省份的供试材料中黑龙江群体材料对灰斑病具有较高的抗性,平均病情指数为52.0%;在黑龙江品种资源的7个主要类型中‘,垦农号’系列群体表现对灰斑病最高的抗性,93.8%品种对灰斑病具有不同程度的抗病性;其次是‘垦鉴豆号’系列和‘绥农号’系列,抗病性品种占比分别为88.2%和85.7%;同时筛选出10份对灰斑病高抗的黑龙江品种,分别为‘垦农19号’‘、垦农17号’‘、黑农43号’‘、黑农47号’‘、垦鉴豆4号’‘、垦鉴豆33’‘、绥农11号’‘、绥农12号’‘、绥农15号’和‘东农43号’。大豆种质资源灰斑病的抗病性保留着丰富的遗传变异;10个高抗品种可以作为培育抗灰斑病品种的资源材料。
The objective is to excavate excellent germplasm resources for cultivating high resistance to Cercospora sojina. 1164 accessions of soybean germplasm resources(915 domestic, 145 overseas and 104 wild)were used as materials, and the resistance was identified by inoculating Cercospora sojina pathogen artificially.The average disease index of the 1164 accessions to Cercospora sojina was 52.2%, and the coefficient of genetic variation was 23.9%. 64.9% of all the varieties had the resistance to Cercospora sojina.‘Chengduo 6' had the strongest resistance to Cercospora sojina, and the disease index was 10%. The resistance of foreign overseas varieties to Cercospora sojina was higher than that of wild and domestic germplasm resources.Among the test materials from 13 provinces, the average disease index of Heilongjiang varieties to Cercospora sojina was 52.0%, which was relatively high.‘Kennong'series had the highest resistance to Cercospora sojina in 7 main types of soybean varieties in Heilongjiang, 93.8% varieties of‘Kennong'series showed different resistance to Cercospora sojina, followed by‘Kenjiandou'series and‘Suinong'series, the varieties with resistance accounted for 88.2% and 85.7%, respectively. 10 varieties with high resistance to Cercospora sojina in Heilongjiang were selected, which were‘Kennong 19',‘Kennong 17',‘Heinong 43',‘Heinong 47',‘Kenjiandou 4',‘Kenjiandou 33',‘Suinong 11',‘Suinong 12',‘Suinong 15' and ‘Dongnong 43'. The resistance of soybean germplasm resources to Cercospora sojina retained abundant genetic variation, and 10 varieties could be used as germplasm resources to cultivate varieties with resistance to Cercospora sojina.
The objective is to excavate excellent germplasm resources for cultivating high resistance to Cercospora sojina. 1164 accessions of soybean germplasm resources(915 domestic, 145 overseas and 104 wild)were used as materials, and the resistance was identified by inoculating Cercospora sojina pathogen artificially.The average disease index of the 1164 accessions to Cercospora sojina was 52.2%, and the coefficient of genetic variation was 23.9%. 64.9% of all the varieties had the resistance to Cercospora sojina.‘Chengduo 6' had the strongest resistance to Cercospora sojina, and the disease index was 10%. The resistance of foreign overseas varieties to Cercospora sojina was higher than that of wild and domestic germplasm resources.Among the test materials from 13 provinces, the average disease index of Heilongjiang varieties to Cercospora sojina was 52.0%, which was relatively high.‘Kennong'series had the highest resistance to Cercospora sojina in 7 main types of soybean varieties in Heilongjiang, 93.8% varieties of‘Kennong'series showed different resistance to Cercospora sojina, followed by‘Kenjiandou'series and‘Suinong'series, the varieties with resistance accounted for 88.2% and 85.7%, respectively. 10 varieties with high resistance to Cercospora sojina in Heilongjiang were selected, which were‘Kennong 19',‘Kennong 17',‘Heinong 43',‘Heinong 47',‘Kenjiandou 4',‘Kenjiandou 33',‘Suinong 11',‘Suinong 12',‘Suinong 15' and ‘Dongnong 43'. The resistance of soybean germplasm resources to Cercospora sojina retained abundant genetic variation, and 10 varieties could be used as germplasm resources to cultivate varieties with resistance to Cercospora sojina.
引文
[1]廖林.大豆灰斑病研究概况及展望[J].中国农学通报,1992,8(1):6-9.
[2]马淑梅.黑龙江省大豆灰斑病研究现状[J].植物保护,1992,18(4):34-36.
[3]任有科,于宝泉.大豆灰斑病研究进展[J].现代农业科技,2010(3):164-165,169.
[4]韩新华,许艳丽,李春杰,等.大豆灰斑病的发生与综合防治[J].大豆科技,2007(3):14-16.
[5]马淑梅.大豆种质资源对灰斑病抗性鉴定评价[J].植物遗传资源学报,2011,12(5):820-824.
[6]朱振东,霍云龙,王晓鸣,等.大豆疫霉根腐病抗源筛选[J].植物遗传资源学报,2006,7(1):24-30.
[7]马淑梅,李宝英.绥化地区大豆灰斑病菌生理小种消长变化的研究[J].大豆科学,1994,13(4):281-285.
[8]朱希敏,王利财,邹桂珍.大豆品种资源抗病毒病、灰斑病和霜霉病鉴定[J].大豆科学,1988,7(3):223-229.
[9]吴秀红,文景芝,马淑梅,等.黑龙江省大豆新品系抗灰斑病情况分析[J].大豆科学,2004,23(2):114-117.
[10]齐宁.大豆品种资源对灰斑病抗性鉴定结果初报[J].黑龙江农业科学,1987(5):25-27.
[11]曹越平,杨庆凯.大豆抗灰斑病的抗性与抗病遗传育种研究的回顾[J].大豆科学,2002,4(21):285-289.
[12]方中达.植病研究方法(第三版)[M].北京:中国农业出版社,1998.
[13]马淑梅.大豆种质资源对灰斑病抗性评价和广谱抗源鉴定[J].中国农学通报,2011,27(17):260-264.
[14]王德亮,胡炳志.中国大豆灰斑病研究现状与抗病育种进展[J].大豆科技,1995(3):14-15.
[15]郭泰,刘忠堂,胡喜平,等.国外大豆种质资源的引入、研究和利用[J].作物杂志,2005(1):62-64.
[16]齐宁,郭泰.美国大豆种质资源在抗灰斑病育种中的利用[J].作物品种资源,1995(4):38-39.
[17]刘忠堂.解决我省东部地区大豆灰斑病的途径[J].黑龙江农业科技,1985(1):20-22.
[18]郭泰,刘忠堂,齐宁,等.大豆抗灰斑病新种质的创新和利用[J].大豆科技,1997(2):18.
[19]马淑梅,李宝英,高学文,等.黑龙江省即将推广的大豆新品种(系)对灰斑病抗病性鉴定[J].大豆科学,1993,12(2):154-158.
[20]陈庆山.大豆灰斑病种质资源遗传多样性的RAPD和SSR分析[D].哈尔滨:东北农业大学,2001.
[21]张丽娟,杨庆凯.大豆抗灰斑病菌多个生理小种资源的筛选[J].大豆科学,1997,16(1):38-41.
[22]马淑梅,韩新华,邵红涛.大豆主要病害多抗性资源筛选鉴定[J].中国农学通报,2014,30(27):58-65.
[23]顾鑫,丁俊杰.大豆灰斑病的研究现状[J].中国农学通报,2010,26(9):303-306.
[2]马淑梅.黑龙江省大豆灰斑病研究现状[J].植物保护,1992,18(4):34-36.
[3]任有科,于宝泉.大豆灰斑病研究进展[J].现代农业科技,2010(3):164-165,169.
[4]韩新华,许艳丽,李春杰,等.大豆灰斑病的发生与综合防治[J].大豆科技,2007(3):14-16.
[5]马淑梅.大豆种质资源对灰斑病抗性鉴定评价[J].植物遗传资源学报,2011,12(5):820-824.
[6]朱振东,霍云龙,王晓鸣,等.大豆疫霉根腐病抗源筛选[J].植物遗传资源学报,2006,7(1):24-30.
[7]马淑梅,李宝英.绥化地区大豆灰斑病菌生理小种消长变化的研究[J].大豆科学,1994,13(4):281-285.
[8]朱希敏,王利财,邹桂珍.大豆品种资源抗病毒病、灰斑病和霜霉病鉴定[J].大豆科学,1988,7(3):223-229.
[9]吴秀红,文景芝,马淑梅,等.黑龙江省大豆新品系抗灰斑病情况分析[J].大豆科学,2004,23(2):114-117.
[10]齐宁.大豆品种资源对灰斑病抗性鉴定结果初报[J].黑龙江农业科学,1987(5):25-27.
[11]曹越平,杨庆凯.大豆抗灰斑病的抗性与抗病遗传育种研究的回顾[J].大豆科学,2002,4(21):285-289.
[12]方中达.植病研究方法(第三版)[M].北京:中国农业出版社,1998.
[13]马淑梅.大豆种质资源对灰斑病抗性评价和广谱抗源鉴定[J].中国农学通报,2011,27(17):260-264.
[14]王德亮,胡炳志.中国大豆灰斑病研究现状与抗病育种进展[J].大豆科技,1995(3):14-15.
[15]郭泰,刘忠堂,胡喜平,等.国外大豆种质资源的引入、研究和利用[J].作物杂志,2005(1):62-64.
[16]齐宁,郭泰.美国大豆种质资源在抗灰斑病育种中的利用[J].作物品种资源,1995(4):38-39.
[17]刘忠堂.解决我省东部地区大豆灰斑病的途径[J].黑龙江农业科技,1985(1):20-22.
[18]郭泰,刘忠堂,齐宁,等.大豆抗灰斑病新种质的创新和利用[J].大豆科技,1997(2):18.
[19]马淑梅,李宝英,高学文,等.黑龙江省即将推广的大豆新品种(系)对灰斑病抗病性鉴定[J].大豆科学,1993,12(2):154-158.
[20]陈庆山.大豆灰斑病种质资源遗传多样性的RAPD和SSR分析[D].哈尔滨:东北农业大学,2001.
[21]张丽娟,杨庆凯.大豆抗灰斑病菌多个生理小种资源的筛选[J].大豆科学,1997,16(1):38-41.
[22]马淑梅,韩新华,邵红涛.大豆主要病害多抗性资源筛选鉴定[J].中国农学通报,2014,30(27):58-65.
[23]顾鑫,丁俊杰.大豆灰斑病的研究现状[J].中国农学通报,2010,26(9):303-306.